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RecombinantRabMAb

Recombinant Anti-GABA A Receptor alpha 1 antibody [EPR5401(2)] (ab151573)

Submit a review Submit a question References (1)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABA A Receptor alpha 1 antibody [EPR5401(2)] (ab151573)
  • Western blot - Anti-GABA A Receptor alpha 1 antibody [EPR5401(2)] (ab151573)
  • Western blot - Anti-GABA A Receptor alpha 1 antibody [EPR5401(2)] (ab151573)
  • Western blot - Anti-GABA A Receptor alpha 1 antibody [EPR5401(2)] (ab151573)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABA A Receptor alpha 1 antibody [EPR5401(2)] (ab151573)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABA A Receptor alpha 1 antibody [EPR5401(2)] (ab151573)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABA A Receptor alpha 1 antibody [EPR5401(2)] (ab151573)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABA A Receptor alpha 1 antibody [EPR5401(2)] (ab151573)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABA A Receptor alpha 1 antibody [EPR5401(2)] (ab151573)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABA A Receptor alpha 1 antibody [EPR5401(2)] (ab151573)
  • Anti-GABA A Receptor alpha 1 antibody [EPR5401(2)] (ab151573)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR5401(2)] to GABA A Receptor alpha 1
  • Suitable for: WB, IHC-P
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

Overview

  • Product name

    Anti-GABA A Receptor alpha 1 antibody [EPR5401(2)]
    See all GABA A Receptor alpha 1 primary antibodies

  • Description

    Rabbit monoclonal [EPR5401(2)] to GABA A Receptor alpha 1

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-Pmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide within Human GABA A Receptor alpha 1 aa 1-100. The exact sequence is proprietary.

  • Positive control

    • WB: Human fetal brain, Human lung and Human cerebellum lysates; Human brain prepared in 1%SDS Hot lysis method. IHC-P: Human brain, colon, kidney, Ovarian carcinoma, spleen, cerebrum and glioma tissues.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab151573 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/1000 - 1/10000. Detects a band of approximately 52 kDa (predicted molecular weight: 52 kDa).

We recommend to try 1% SDS Hot lysis prepare method to get desired WB results.

For Lysate preparation protocol, please refer to the protocol book in the protocol section and/or here (downloadable copy).
IHC-P
1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

For unpurified use 1/50 - 1/100

See IHC antigen retrieval protocols.
Notes
WB
1/1000 - 1/10000. Detects a band of approximately 52 kDa (predicted molecular weight: 52 kDa).

We recommend to try 1% SDS Hot lysis prepare method to get desired WB results.

For Lysate preparation protocol, please refer to the protocol book in the protocol section and/or here (downloadable copy).
IHC-P
1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

For unpurified use 1/50 - 1/100

See IHC antigen retrieval protocols.

Target

  • Function

    GABA, the major inhibitory neurotransmitter in the vertebrate brain, mediates neuronal inhibition by binding to the GABA/benzodiazepine receptor and opening an integral chloride channel.

  • Involvement in disease

    Defects in GABRA1 are the cause of childhood absence epilepsy type 4 (ECA4) [MIM:611136]. A subtype of idiopathic generalized epilepsy characterized by onset at age 6-7 years, frequent absence seizures (several per day) and bilateral, synchronous, symmetric 3-Hz spike waves on EEG. During adolescence, tonic-clonic and myoclonic seizures may develop. Absence seizures may either remit or persist into adulthood.
    Defects in GABRA1 are the cause of juvenile myoclonic epilepsy type 5 (EJM5) [MIM:611136]. A subtype of idiopathic generalized epilepsy. Patients have afebrile seizures only, with onset in adolescence (rather than in childhood) and myoclonic jerks which usually occur after awakening and are triggered by sleep deprivation and fatigue.

  • Sequence similarities

    Belongs to the ligand-gated ion channel (TC 1.A.9) family. Gamma-aminobutyric acid receptor (TC 1.A.9.5) subfamily. GABRA1 sub-subfamily.

  • Cellular localization

    Cell junction > synapse > postsynaptic cell membrane. Cell membrane.
  • Information by UniProt

  • Database links

    • Alternative names

      • ECA4 antibody
      • EIEE19 antibody
      • EJM antibody
      • EJM5 antibody
      • Gaba receptor alpha 1 polypeptide antibody
      • GABA(A) receptor antibody
      • GABA(A) receptor subunit alpha 1 antibody
      • GABA(A) receptor subunit alpha-1 antibody
      • GABA(A) receptor, alpha 1 antibody
      • GABRA 1 antibody
      • GABRA1 antibody
      • Gamma aminobutyric acid (GABA) A receptor alpha 1 antibody
      • Gamma aminobutyric acid A receptor alpha 1 antibody
      • Gamma aminobutyric acid receptor subunit alpha 1 antibody
      • Gamma aminobutyric acid type A receptor alpha1 subunit antibody
      • Gamma-aminobutyric acid receptor subunit alpha-1 antibody
      • GBRA1_HUMAN antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABA A Receptor alpha 1 antibody [EPR5401(2)] (ab151573)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABA A Receptor alpha 1 antibody [EPR5401(2)] (ab151573)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human cerebrum tissue sections labeling GABA A Receptor alpha 1 with Purified ab151573 at 1:1000 dilution (0.7 µg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0)ImmunoHistoProbe one step HRP Polymer (ready to use)was used as the secondary antibody.Negative control:PBS instead of the primary antibody.Hematoxylinwas used as a counterstain
    • Western blot - Anti-GABA A Receptor alpha 1 antibody [EPR5401(2)] (ab151573)
      Western blot - Anti-GABA A Receptor alpha 1 antibody [EPR5401(2)] (ab151573)
      All lanes : Anti-GABA A Receptor alpha 1 antibody [EPR5401(2)] (ab151573) at 1/1000 dilution

      Lane 1 : Human brain prepared in RIPA lysis method
      Lane 2 : Human brain prepared in 1%SDS Hot lysis method

      Lysates/proteins at 15 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

      Developed using the ECL technique.

      Predicted band size: 52 kDa
      Observed band size: 52 kDa


      Exposure time: 40 seconds


      Blocking/Diluting buffer and concentration: 5% NFDM/TBST

      We recommend to use 1%SDS Hot lysis prepare method to get desired Western Blot results.

    • Western blot - Anti-GABA A Receptor alpha 1 antibody [EPR5401(2)] (ab151573)
      Western blot - Anti-GABA A Receptor alpha 1 antibody [EPR5401(2)] (ab151573)
      All lanes : Anti-GABA A Receptor alpha 1 antibody [EPR5401(2)] (ab151573) at 0.7 µg/ml (purified)

      Lane 1 : Human brain lysates
      Lane 2 : Human lung lysates - negative control

      Lysates/proteins at 15 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

      Predicted band size: 52 kDa



      Blocking and diluting buffer: 5% NFDM/TBST.


      Please note: Lysates prepared in 1%SDS Hot lysis method.

      According to the paper (PMID: 29467616), brain is positive and lung is negative tissue.

    • Western blot - Anti-GABA A Receptor alpha 1 antibody [EPR5401(2)] (ab151573)
      Western blot - Anti-GABA A Receptor alpha 1 antibody [EPR5401(2)] (ab151573)
      All lanes : Anti-GABA A Receptor alpha 1 antibody [EPR5401(2)] (ab151573) at 1/1000 dilution (unpurified)

      Lane 1 : Human fetal brain lysate
      Lane 2 : Human cerebellum lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size: 52 kDa



      Please note: Lysates prepared in 1%SDS Hot lysis method.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABA A Receptor alpha 1 antibody [EPR5401(2)] (ab151573)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABA A Receptor alpha 1 antibody [EPR5401(2)] (ab151573)

      Immunohistochemical analysis of paraffin-embedded Human brain tissue labeling GABA A Receptor alpha 1 with unpurified ab151573 at 1/50 dilution.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABA A Receptor alpha 1 antibody [EPR5401(2)] (ab151573)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABA A Receptor alpha 1 antibody [EPR5401(2)] (ab151573)

      Immunohistochemical analysis of paraffin-embedded Human glioma tissue labeling GABA A Receptor alpha 1 with unpurified ab151573 at 1/50 dilution.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABA A Receptor alpha 1 antibody [EPR5401(2)] (ab151573)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABA A Receptor alpha 1 antibody [EPR5401(2)] (ab151573)

      Immunohistochemical analysis of paraffin embedded normal Human colon tissue using unpurified ab151573 showing -ve staining.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABA A Receptor alpha 1 antibody [EPR5401(2)] (ab151573)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABA A Receptor alpha 1 antibody [EPR5401(2)] (ab151573)

      Immunohistochemical analysis of paraffin embedded normal Human kidney tissue using unpurified ab151573 showing -ve staining.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABA A Receptor alpha 1 antibody [EPR5401(2)] (ab151573)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABA A Receptor alpha 1 antibody [EPR5401(2)] (ab151573)

      Immunohistochemical analysis of paraffin embedded Human Ovarian carcinoma tissue using unpurified ab151573 showing -ve staining.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABA A Receptor alpha 1 antibody [EPR5401(2)] (ab151573)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABA A Receptor alpha 1 antibody [EPR5401(2)] (ab151573)

      Immunohistochemical analysis of paraffin embedded normal Human spleen tissue using unpurified ab151573 showing -ve staining.

    • Anti-GABA A Receptor alpha 1 antibody [EPR5401(2)] (ab151573)
      Anti-GABA A Receptor alpha 1 antibody [EPR5401(2)] (ab151573)

    References (1)

    Publishing research using ab151573? Please let us know so that we can cite the reference in this datasheet.

    ab151573 has been referenced in 1 publication.

    • Tabassum S  et al. Jujuboside A prevents sleep loss-induced disturbance of hippocampal neuronal excitability and memory impairment in young APP/PS1 mice. Sci Rep 9:4512 (2019). PubMed: 30872728

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