Recombinant Anti-GABA A Receptor alpha 1 antibody [EPR5401(2)] (ab151573)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR5401(2)] to GABA A Receptor alpha 1
- Suitable for: WB, IHC-P
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-GABA A Receptor alpha 1 antibody [EPR5401(2)]
See all GABA A Receptor alpha 1 primary antibodies -
Description
Rabbit monoclonal [EPR5401(2)] to GABA A Receptor alpha 1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human GABA A Receptor alpha 1 aa 1-100. The exact sequence is proprietary.
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Positive control
- WB: Human fetal brain, Human lung and Human cerebellum lysates; Human brain prepared in 1%SDS Hot lysis method. IHC-P: Human brain, colon, kidney, Ovarian carcinoma, spleen, cerebrum and glioma tissues.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR5401(2) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab151573 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000 - 1/10000. Detects a band of approximately 52 kDa (predicted molecular weight: 52 kDa).
We recommend to try 1% SDS Hot lysis prepare method to get desired WB results. For Lysate preparation protocol, please refer to the protocol book in the protocol section and/or here (downloadable copy). |
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IHC-P |
1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
For unpurified use 1/50 - 1/100 |
Notes |
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WB
1/1000 - 1/10000. Detects a band of approximately 52 kDa (predicted molecular weight: 52 kDa). We recommend to try 1% SDS Hot lysis prepare method to get desired WB results. For Lysate preparation protocol, please refer to the protocol book in the protocol section and/or here (downloadable copy). |
IHC-P
1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. For unpurified use 1/50 - 1/100 |
Target
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Function
GABA, the major inhibitory neurotransmitter in the vertebrate brain, mediates neuronal inhibition by binding to the GABA/benzodiazepine receptor and opening an integral chloride channel. -
Involvement in disease
Defects in GABRA1 are the cause of childhood absence epilepsy type 4 (ECA4) [MIM:611136]. A subtype of idiopathic generalized epilepsy characterized by onset at age 6-7 years, frequent absence seizures (several per day) and bilateral, synchronous, symmetric 3-Hz spike waves on EEG. During adolescence, tonic-clonic and myoclonic seizures may develop. Absence seizures may either remit or persist into adulthood.
Defects in GABRA1 are the cause of juvenile myoclonic epilepsy type 5 (EJM5) [MIM:611136]. A subtype of idiopathic generalized epilepsy. Patients have afebrile seizures only, with onset in adolescence (rather than in childhood) and myoclonic jerks which usually occur after awakening and are triggered by sleep deprivation and fatigue. -
Sequence similarities
Belongs to the ligand-gated ion channel (TC 1.A.9) family. Gamma-aminobutyric acid receptor (TC 1.A.9.5) subfamily. GABRA1 sub-subfamily. -
Cellular localization
Cell junction > synapse > postsynaptic cell membrane. Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 2554 Human
- Omim: 137160 Human
- SwissProt: P14867 Human
- Unigene: 175934 Human
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Alternative names
- ECA4 antibody
- EIEE19 antibody
- EJM antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABA A Receptor alpha 1 antibody [EPR5401(2)] (ab151573)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human cerebrum tissue sections labeling GABA A Receptor alpha 1 with Purified ab151573 at 1:1000 dilution (0.7 µg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0)ImmunoHistoProbe one step HRP Polymer (ready to use)was used as the secondary antibody.Negative control:PBS instead of the primary antibody.Hematoxylinwas used as a counterstain
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All lanes : Anti-GABA A Receptor alpha 1 antibody [EPR5401(2)] (ab151573) at 1/1000 dilution
Lane 1 : Human brain prepared in RIPA lysis method
Lane 2 : Human brain prepared in 1%SDS Hot lysis method
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Developed using the ECL technique.
Predicted band size: 52 kDa
Observed band size: 52 kDa
Exposure time: 40 secondsBlocking/Diluting buffer and concentration: 5% NFDM/TBST
We recommend to use 1%SDS Hot lysis prepare method to get desired Western Blot results.
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All lanes : Anti-GABA A Receptor alpha 1 antibody [EPR5401(2)] (ab151573) at 0.7 µg/ml (purified)
Lane 1 : Human brain lysates
Lane 2 : Human lung lysates - negative control
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Predicted band size: 52 kDaBlocking and diluting buffer: 5% NFDM/TBST.
Please note: Lysates prepared in 1%SDS Hot lysis method.According to the paper (PMID: 29467616), brain is positive and lung is negative tissue.
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All lanes : Anti-GABA A Receptor alpha 1 antibody [EPR5401(2)] (ab151573) at 1/1000 dilution (unpurified)
Lane 1 : Human fetal brain lysate
Lane 2 : Human cerebellum lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 52 kDaPlease note: Lysates prepared in 1%SDS Hot lysis method.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABA A Receptor alpha 1 antibody [EPR5401(2)] (ab151573)
Immunohistochemical analysis of paraffin-embedded Human brain tissue labeling GABA A Receptor alpha 1 with unpurified ab151573 at 1/50 dilution.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABA A Receptor alpha 1 antibody [EPR5401(2)] (ab151573)
Immunohistochemical analysis of paraffin-embedded Human glioma tissue labeling GABA A Receptor alpha 1 with unpurified ab151573 at 1/50 dilution.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABA A Receptor alpha 1 antibody [EPR5401(2)] (ab151573)
Immunohistochemical analysis of paraffin embedded normal Human colon tissue using unpurified ab151573 showing -ve staining.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABA A Receptor alpha 1 antibody [EPR5401(2)] (ab151573)
Immunohistochemical analysis of paraffin embedded normal Human kidney tissue using unpurified ab151573 showing -ve staining.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABA A Receptor alpha 1 antibody [EPR5401(2)] (ab151573)
Immunohistochemical analysis of paraffin embedded Human Ovarian carcinoma tissue using unpurified ab151573 showing -ve staining.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABA A Receptor alpha 1 antibody [EPR5401(2)] (ab151573)
Immunohistochemical analysis of paraffin embedded normal Human spleen tissue using unpurified ab151573 showing -ve staining.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab151573 has been referenced in 1 publication.
- Tabassum S et al. Jujuboside A prevents sleep loss-induced disturbance of hippocampal neuronal excitability and memory impairment in young APP/PS1 mice. Sci Rep 9:4512 (2019). PubMed: 30872728