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Anti-GABA A Receptor beta 3/GABRB3 antibody [N87/25] (ab98968)

Submit a review Q&A (2)References (14)
Western blot - Anti-GABA A Receptor beta 3/GABRB3 antibody [N87/25] (ab98968)
  • Immunohistochemistry (Frozen sections) - Anti-GABA A Receptor beta 3/GABRB3 antibody [N87/25] (ab98968)
  • Flow Cytometry - Anti-GABA A Receptor beta 3/GABRB3 antibody [N87/25] (ab98968)

Key features and details

  • Mouse monoclonal [N87/25] to GABA A Receptor beta 3/GABRB3
  • Suitable for: IHC-Fr, ICC/IF, IHC-P, Flow Cyt, WB
  • Reacts with: Mouse, Rat, Human
  • Isotype: IgG1

Overview

  • Product name

    Anti-GABA A Receptor beta 3/GABRB3 antibody [N87/25]
    See all GABA A Receptor beta 3/GABRB3 primary antibodies

  • Description

    Mouse monoclonal [N87/25] to GABA A Receptor beta 3/GABRB3

  • Host species

    Mouse

  • Specificity

    Does not cross react with GABA A Receptor Beta 2 or Beta 1.

  • Tested applications

    Suitable for: IHC-Fr, ICC/IF, IHC-P, Flow Cyt, WBmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human
    Predicted to work with: Cow, Orangutan

  • Immunogen

    Fusion protein corresponding to Mouse GABA A Receptor beta 3/GABRB3 aa 350-450.
    Database link: P63080

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • Positive control

    • WB: Rat brain lysate. Flow Cyt: SH-SY5Y cells. IHC-P: Mouse brain tissue.

  • General notes

    The clone number has been updated from S87-25 to N87/25, both clone numbers name the same antibody clone.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab98968 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr
1/1000.
ICC/IF
Use at an assay dependent concentration.
IHC-P
Use at an assay dependent concentration.
Flow Cyt
Use 1µg for 106 cells.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
WB
1/1000. Predicted molecular weight: 55 kDa.

Sufficient for detection of GABA A Receptor beta 3/GABRB3 in 10µg of rat brain lysate by colorimetric immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.
Notes
IHC-Fr
1/1000.
ICC/IF
Use at an assay dependent concentration.
IHC-P
Use at an assay dependent concentration.
Flow Cyt
Use 1µg for 106 cells.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
WB
1/1000. Predicted molecular weight: 55 kDa.

Sufficient for detection of GABA A Receptor beta 3/GABRB3 in 10µg of rat brain lysate by colorimetric immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.

Target

  • Function

    Component of the heteropentameric receptor for GABA, the major inhibitory neurotransmitter in the vertebrate brain. Functions also as histamine receptor and mediates cellular responses to histamine. Functions as receptor for diazepines and various anesthetics, such as pentobarbital; these are bound at a separate allosteric effector binding site. Functions as ligand-gated chloride channel.

  • Involvement in disease

    Epilepsy, childhood absence 5

  • Sequence similarities

    Belongs to the ligand-gated ion channel (TC 1.A.9) family. Gamma-aminobutyric acid receptor (TC 1.A.9.5) subfamily. GABRB3 sub-subfamily.

  • Cellular localization

    Cell junction, synapse, postsynaptic cell membrane. Cell membrane.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • ECA5 antibody
    • GABA alpha receptor beta-2 subunit antibody
    • GABA(A) receptor subunit beta-3 antibody
    • GABAA receptor beta 3 subunit antibody
    • GABAA receptor subunit beta 3 antibody
    • GABR B3 antibody
    • Gabrb3 antibody
    • Gamma aminobutyric acid (GABA) A receptor beta 3 antibody
    • Gamma aminobutyric acid receptor subunit beta 3 antibody
    • Gamma-aminobutyric acid receptor subunit beta-3 antibody
    • GBRB3_HUMAN antibody
    • MGC9051 antibody
    see all

Images

  • Western blot - Anti-GABA A Receptor beta 3/GABRB3 antibody [N87/25] (ab98968)
    Western blot - Anti-GABA A Receptor beta 3/GABRB3 antibody [N87/25] (ab98968)
    Anti-GABA A Receptor beta 3/GABRB3 antibody [N87/25] (ab98968) at 1/1000 dilution + Rat brain membrane lysate at 15 µg

    Secondary
    Sheep Anti-Mouse IgG: HRP

    Predicted band size: 55 kDa



    Block: 1.5% BSA for 30 minutes at RT.

    Incubation with primary antibody for 2 hours at RT.

    Incubation with secondary antibody for 1 hour at RT.

  • Immunohistochemistry (Frozen sections) - Anti-GABA A Receptor beta 3/GABRB3 antibody [N87/25] (ab98968)
    Immunohistochemistry (Frozen sections) - Anti-GABA A Receptor beta 3/GABRB3 antibody [N87/25] (ab98968)

    ab98968 staining GABA A Receptor beta 3/GABRB3 in mouse brain tissue sections by Immunohistochemistry (IHC-Fr - frozen sections).

    Tissue was fixed in 10% formalin for 12-24 hours at RT. Primary Antibody: ab98968 at 1:1000 for 1 hour at RT. Secondary Antibody: HRP/DAB Detection System: Biotinylated Goat Anti-Mouse, Streptavidin Peroxidase, DAB Chromogen (brown) for 30 minutes at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 250-500 µl for 5 minutes at RT.

  • Flow Cytometry - Anti-GABA A Receptor beta 3/GABRB3 antibody [N87/25] (ab98968)
    Flow Cytometry - Anti-GABA A Receptor beta 3/GABRB3 antibody [N87/25] (ab98968)

    Overlay histogram showing SH-SY5Y (Human neuroblastoma cell line from bone marrow) cells stained with ab98968 (red line).

    The cells were fixed with 4% paraformaldehyde (10 minutes) and then permeabilized with 0.1% PBS-Tween for 20 minutes. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab98968, 1µg/1x106 cells) for 30 minutes at 22ºC.

    The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 minutes at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions.

    Acquisition of >5,000 events was performed. This antibody gave a positive signal in SH-SY5Y cells fixed with 80% methanol (5 minutes)/permeabilized with 0.1% PBS-Tween for 20 minutes used under the same conditions.

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

References (14)

Publishing research using ab98968? Please let us know so that we can cite the reference in this datasheet.

ab98968 has been referenced in 14 publications.

  • Menzikov SA  et al. Physiological Role of ATPase for GABAA Receptor Resensitization. Int J Mol Sci 23:N/A (2022). PubMed: 35628132
  • Chu MC  et al. Generational synaptic functions of GABAA receptor β3 subunit deteriorations in an animal model of social deficit. J Biomed Sci 29:51 (2022). PubMed: 35821032
  • Liu Q  et al. Anesthetic Propofol Promotes Tumor Metastasis in Lungs via GABAA R-Dependent TRIM21 Modulation of Src Expression. Adv Sci (Weinh) 8:e2102079 (2021). PubMed: 34263559
  • Farmer GE  et al. AT1a-dependent GABAA inhibition in the MnPO following chronic intermittent hypoxia. Am J Physiol Regul Integr Comp Physiol 321:R469-R481 (2021). PubMed: 34189959
  • Victor AK  et al. Molecular Changes in Prader-Willi Syndrome Neurons Reveals Clues About Increased Autism Susceptibility. Front Mol Neurosci 14:747855 (2021). PubMed: 34776864
View all Publications for this product

Customer reviews and Q&As

Show All Reviews Q&A
Submit a review Submit a question

1-2 of 2 Abreviews or Q&A

Question

I was wondering if you could look at my western blot and tell me which band is the band for gabaAbeta 3. The predicted MW is 55 kDa. There are 2 bands close to 50kDa.

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Answer

According to uniprot (link below) there are four isoforms of GabaA Beta3, all of which ab98968 is capable of detecting. Isoforms 1 and 2 are ˜54 kDa, isoform 3 is 47 kDa, and isoform 4 is 45 kDa. Since GabaA Beta3 is glycosylated, I would suspect the stronger, higher band is isoform 1/2 and the lower more faint band corresponds to isoforms 3 or 4.

http://www.uniprot.org/uniprot/P28472

Read More

Question

Dear ,
Thank you very much for your response. I understand your position.
As I want to study a lot of paraffin slides, I would like to know if I can get a small aliquot of the ab98968 antibody that it seems suitable for IHC-P before purchasing it just to be sure that it works.
Best regards.

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Answer

Thank you very much for your comprehension, it’s really appreciated.
Unfortunately, because we carry over 80,000 products, it isn't feasible for us to keep small sample sizes of our products, and therefore I am not able to offer a test sample of ab98968.
However, in this case, ab98968 is guaranteed to work in IHC-P in human, rat and mouse.
If you had any problem using this antibody, please contact us. It is covered by our Abpromise® guarantee, which ensures that you can trust our products, and they should work in the tested species and applications stated on the datasheet, or we will offer a replacement, credit, or refund, if reported within 6 months of purchase.
To read about our Abpromise policy: https://www.abcam.com/index.html?pageconfig=abpromise
I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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