Anti-GABA A Receptor beta 3/GABRB3 antibody [N87/25] (ab98968)
Key features and details
- Mouse monoclonal [N87/25] to GABA A Receptor beta 3/GABRB3
- Suitable for: IHC-Fr, ICC/IF, IHC-P, Flow Cyt, WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG1
Overview
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Product name
Anti-GABA A Receptor beta 3/GABRB3 antibody [N87/25]
See all GABA A Receptor beta 3/GABRB3 primary antibodies -
Description
Mouse monoclonal [N87/25] to GABA A Receptor beta 3/GABRB3 -
Host species
Mouse -
Specificity
Does not cross react with GABA A Receptor Beta 2 or Beta 1. -
Tested applications
Suitable for: IHC-Fr, ICC/IF, IHC-P, Flow Cyt, WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human
Predicted to work with: Cow, Orangutan -
Immunogen
Fusion protein corresponding to Mouse GABA A Receptor beta 3/GABRB3 aa 350-450.
Database link: P63080 -
Positive control
- WB: Rat brain lysate. Flow Cyt: SH-SY5Y cells. IHC-P: Mouse brain tissue.
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General notes
The clone number has been updated from S87-25 to N87/25, both clone numbers name the same antibody clone.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
N87/25 -
Isotype
IgG1 -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab98968 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-Fr |
1/1000.
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ICC/IF |
Use at an assay dependent concentration.
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IHC-P |
Use at an assay dependent concentration.
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Flow Cyt |
Use 1µg for 106 cells.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
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WB |
1/1000. Predicted molecular weight: 55 kDa.
Sufficient for detection of GABA A Receptor beta 3/GABRB3 in 10µg of rat brain lysate by colorimetric immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody. |
Notes |
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IHC-Fr
1/1000. |
ICC/IF
Use at an assay dependent concentration. |
IHC-P
Use at an assay dependent concentration. |
Flow Cyt
Use 1µg for 106 cells. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
WB
1/1000. Predicted molecular weight: 55 kDa. Sufficient for detection of GABA A Receptor beta 3/GABRB3 in 10µg of rat brain lysate by colorimetric immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody. |
Target
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Function
Component of the heteropentameric receptor for GABA, the major inhibitory neurotransmitter in the vertebrate brain. Functions also as histamine receptor and mediates cellular responses to histamine. Functions as receptor for diazepines and various anesthetics, such as pentobarbital; these are bound at a separate allosteric effector binding site. Functions as ligand-gated chloride channel. -
Involvement in disease
Epilepsy, childhood absence 5 -
Sequence similarities
Belongs to the ligand-gated ion channel (TC 1.A.9) family. Gamma-aminobutyric acid receptor (TC 1.A.9.5) subfamily. GABRB3 sub-subfamily. -
Cellular localization
Cell junction, synapse, postsynaptic cell membrane. Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 2562 Human
- Entrez Gene: 14402 Mouse
- Entrez Gene: 24922 Rat
- Omim: 137192 Human
- SwissProt: P28472 Human
- SwissProt: P63080 Mouse
- SwissProt: P63079 Rat
- Unigene: 302352 Human
see all -
Alternative names
- ECA5 antibody
- GABA alpha receptor beta-2 subunit antibody
- GABA(A) receptor subunit beta-3 antibody
see all
Images
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Anti-GABA A Receptor beta 3/GABRB3 antibody [N87/25] (ab98968) at 1/1000 dilution + Rat brain membrane lysate at 15 µg
Secondary
Sheep Anti-Mouse IgG: HRP
Predicted band size: 55 kDaBlock: 1.5% BSA for 30 minutes at RT.
Incubation with primary antibody for 2 hours at RT.
Incubation with secondary antibody for 1 hour at RT.
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Immunohistochemistry (Frozen sections) - Anti-GABA A Receptor beta 3/GABRB3 antibody [N87/25] (ab98968)
ab98968 staining GABA A Receptor beta 3/GABRB3 in mouse brain tissue sections by Immunohistochemistry (IHC-Fr - frozen sections).
Tissue was fixed in 10% formalin for 12-24 hours at RT. Primary Antibody: ab98968 at 1:1000 for 1 hour at RT. Secondary Antibody: HRP/DAB Detection System: Biotinylated Goat Anti-Mouse, Streptavidin Peroxidase, DAB Chromogen (brown) for 30 minutes at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 250-500 µl for 5 minutes at RT.
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Overlay histogram showing SH-SY5Y (Human neuroblastoma cell line from bone marrow) cells stained with ab98968 (red line).
The cells were fixed with 4% paraformaldehyde (10 minutes) and then permeabilized with 0.1% PBS-Tween for 20 minutes. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab98968, 1µg/1x106 cells) for 30 minutes at 22ºC.
The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 minutes at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions.
Acquisition of >5,000 events was performed. This antibody gave a positive signal in SH-SY5Y cells fixed with 80% methanol (5 minutes)/permeabilized with 0.1% PBS-Tween for 20 minutes used under the same conditions.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (14)
ab98968 has been referenced in 14 publications.
- Menzikov SA et al. Physiological Role of ATPase for GABAA Receptor Resensitization. Int J Mol Sci 23:N/A (2022). PubMed: 35628132
- Chu MC et al. Generational synaptic functions of GABAA receptor β3 subunit deteriorations in an animal model of social deficit. J Biomed Sci 29:51 (2022). PubMed: 35821032
- Liu Q et al. Anesthetic Propofol Promotes Tumor Metastasis in Lungs via GABAA R-Dependent TRIM21 Modulation of Src Expression. Adv Sci (Weinh) 8:e2102079 (2021). PubMed: 34263559
- Farmer GE et al. AT1a-dependent GABAA inhibition in the MnPO following chronic intermittent hypoxia. Am J Physiol Regul Integr Comp Physiol 321:R469-R481 (2021). PubMed: 34189959
- Victor AK et al. Molecular Changes in Prader-Willi Syndrome Neurons Reveals Clues About Increased Autism Susceptibility. Front Mol Neurosci 14:747855 (2021). PubMed: 34776864