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RecombinantRabMAb

Recombinant Anti-GABA A Receptor gamma 2/GABRG2 antibody [EPR25325] (ab288564)

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Western blot - Anti-GABA A Receptor gamma 2/GABRG2 antibody [EPR25325-5] (ab288564)
  • Western blot - Anti-GABA A Receptor gamma 2/GABRG2 antibody [EPR25325-5] (ab288564)
  • Immunocytochemistry/ Immunofluorescence - Anti-GABA A Receptor gamma 2/GABRG2 antibody [EPR25325] (ab288564)
  • Immunocytochemistry/ Immunofluorescence - Anti-GABA A Receptor gamma 2/GABRG2 antibody [EPR25325] (ab288564)
  • Flow Cytometry (Intracellular) - Anti-GABA A Receptor gamma 2/GABRG2 antibody [EPR25325-5] (ab288564)
  • Immunoprecipitation - Anti-GABA A Receptor gamma 2/GABRG2 antibody [EPR25325-5] (ab288564)
  • Anti-GABA A Receptor gamma 2/GABRG2 antibody [EPR25325] (ab288564)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR25325-5] to GABA A Receptor gamma 2/GABRG2
  • Suitable for: Flow Cyt (Intra), WB, ICC/IF, IP
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Carrier Free

Overview

  • Product name

    Anti-GABA A Receptor gamma 2/GABRG2 antibody [EPR25325]
    See all GABA A Receptor gamma 2/GABRG2 primary antibodies

  • Description

    Rabbit monoclonal [EPR25325-5] to GABA A Receptor gamma 2/GABRG2

  • Host species

    Rabbit

  • Specificity

    Flow Cyt application does not react with Mouse species.

  • Tested applications

    Suitable for: Flow Cyt (Intra), WB, ICC/IF, IPmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Rat brain, Rat cerebral cortex, Mouse brain, Mouse cerebellum, Neuro-2a, SH-SY5Y, 293T and A-172 lysates. ICC: SH-SY5Y and Neuro-2a cells. Flow Cyt: 293T cells. IP: Mouse brain tissue lysate.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab288564 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt (Intra)
1/50.

FC application does not react with Mouse species.
WB
1/1000. Predicted molecular weight: 54 kDa.
ICC/IF
1/500.
IP
1/30.
Notes
Flow Cyt (Intra)
1/50.

FC application does not react with Mouse species.
WB
1/1000. Predicted molecular weight: 54 kDa.
ICC/IF
1/500.
IP
1/30.

Target

  • Function

    GABA, the major inhibitory neurotransmitter in the vertebrate brain, mediates neuronal inhibition by binding to the GABA/benzodiazepine receptor and opening an integral chloride channel.

  • Involvement in disease

    Defects in GABRG2 are the cause of childhood absence epilepsy type 2 (ECA2) [MIM:607681]. ECA2 is a subtype of idiopathic generalized epilepsy (IGE) characterized by an onset at age 6-7 years, frequent absence seizures (several per day) and bilateral, synchronous, symmetric 3-Hz spike waves on EEG. During adolescence, tonic-clonic and myoclonic seizures develop. Some individuals manifest ECA2 occurring in combination with febrile convulsions.
    Defects in GABRG2 are the cause of familial febrile convulsions type 8 (FEB8) [MIM:611277]. A febrile convulsion is defined as a seizure event in infancy or childhood, usually occurring between 6 months and 6 years of age, associated with fever but without any evidence of intracranial infection or defined pathologic or traumatic cause. Febrile convulsions affect 5-12% of infants and children up to 6 years of age. There is epidemiological evidence that febrile seizures are associated with subsequent afebrile and unprovoked seizures in 2% to 7% of patients.
    Defects in GABRG2 are the cause of generalized epilepsy with febrile seizures plus type 3 (GEFS+3) [MIM:604233]. Generalized epilepsy with febrile seizures-plus refers to a rare autosomal dominant, familial condition with incomplete penetrance and large intrafamilial variability. Patients display febrile seizures persisting sometimes beyond the age of 6 years and/or a variety of afebrile seizure types. GEFS+ is a disease combining febrile seizures, generalized seizures often precipitated by fever at age 6 years or more, and partial seizures, with a variable degree of severity.
    Defects in GABRG2 are a cause of severe myoclonic epilepsy in infancy (SMEI) [MIM:607208]; also called Dravet syndrome. SMEI is a rare disorder characterized by generalized tonic, clonic, and tonic-clonic seizures that are initially induced by fever and begin during the first year of life. Later, patients also manifest other seizure types, including absence, myoclonic, and simple and complex partial seizures. Psychomotor development delay is observed around the second year of life. SMEI is considered to be the most severe phenotype within the spectrum of generalized epilepsies with febrile seizures-plus.

  • Sequence similarities

    Belongs to the ligand-gated ion channel (TC 1.A.9) family. Gamma-aminobutyric acid receptor (TC 1.A.9.5) subfamily. GABRG2 sub-subfamily.

  • Post-translational
    modifications

    Palmitoylated by ZDHHC3/GODZ; which may affect presynaptic clustering and/or cell surface stability.

  • Cellular localization

    Cell junction > synapse > postsynaptic cell membrane. Cell membrane.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • CAE 2 antibody
    • CAE2 antibody
    • ECA 2 antibody
    • ECA2 antibody
    • GABA(A) receptor gamma 2 antibody
    • GABA(A) receptor subunit gamma 2 antibody
    • GABA(A) receptor subunit gamma-2 antibody
    • GABRG 2 antibody
    • GABRG2 antibody
    • Gamma aminobutyric acid (GABA) A receptor gamma 2 antibody
    • Gamma aminobutyric acid A receptor gamma 2 antibody
    • Gamma aminobutyric acid receptor gamma 2 subunit antibody
    • Gamma-aminobutyric acid receptor subunit gamma-2 antibody
    • GBRG2_HUMAN antibody
    • GEFSP 3 antibody
    • GEFSP3 antibody
    see all

Images

  • Western blot - Anti-GABA A Receptor gamma 2/GABRG2 antibody [EPR25325-5] (ab288564)
    Western blot - Anti-GABA A Receptor gamma 2/GABRG2 antibody [EPR25325-5] (ab288564)
    All lanes : Anti-GABA A Receptor gamma 2/GABRG2 antibody [EPR25325] (ab288564) at 1/1000 dilution

    Lane 1 : SH-SY5Y (human neuroblastoma epithelial cell), whole cell lysate
    Lane 2 : HEK-293T (Human embryonic kidney epithelial cell), whole cell lysate
    Lane 3 : A-172 (human brain glioblastoma), whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100000 dilution

    Predicted band size: 54 kDa



    Blocking and diluting buffer and concentration: 5% NFDM/TBST

    Negative control: 293T (PMID: 27864268).

     Exposure time: 147 seconds

     

  • Western blot - Anti-GABA A Receptor gamma 2/GABRG2 antibody [EPR25325-5] (ab288564)
    Western blot - Anti-GABA A Receptor gamma 2/GABRG2 antibody [EPR25325-5] (ab288564)
    All lanes : Anti-GABA A Receptor gamma 2/GABRG2 antibody [EPR25325] (ab288564) at 1/1000 dilution

    Lane 1 : Rat brain tissue lysate
    Lane 2 : Rat cerebral cortex tissue lysate
    Lane 3 : Mouse brain tissue lysate
    Lane 4 : Mouse cerebellum tissue lysate
    Lane 5 : Neuro-2a (mouse neuroblastoma neuroblast), whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100000 dilution

    Predicted band size: 54 kDa



    Blocking and diluting buffer and concentration: 5% NFDM/TBST

    The blot of Lane 3&5 was developed using a higher sensitivity ECL substrate.

     Exposure time: Lane 1, 2 &4: 147 seconds Lane 3 & 5: 3 minutes

     

  • Immunocytochemistry/ Immunofluorescence - Anti-GABA A Receptor gamma 2/GABRG2 antibody [EPR25325] (ab288564)
    Immunocytochemistry/ Immunofluorescence - Anti-GABA A Receptor gamma 2/GABRG2 antibody [EPR25325] (ab288564)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized SH-SY5Y cells labelling GABA A Receptor gamma 2/GABRG2 with ab288564 at 1/500 dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 dilution (Green). Confocal image showing membranous staining in SH-SY5Y cell line.Negative control: 293T (PMID: 27864268） is observed. ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution.

  • Immunocytochemistry/ Immunofluorescence - Anti-GABA A Receptor gamma 2/GABRG2 antibody [EPR25325] (ab288564)
    Immunocytochemistry/ Immunofluorescence - Anti-GABA A Receptor gamma 2/GABRG2 antibody [EPR25325] (ab288564)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized Neuro-2a cells labelling GABA A Receptor gamma 2/GABRG2 with ab288564 at 1/500 dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 dilution (Green). Confocal image showing membranous staining in Neuro-2a cell line. is observed. ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution.

  • Flow Cytometry (Intracellular) - Anti-GABA A Receptor gamma 2/GABRG2 antibody [EPR25325-5] (ab288564)
    Flow Cytometry (Intracellular) - Anti-GABA A Receptor gamma 2/GABRG2 antibody [EPR25325-5] (ab288564)

    Flow cytometric analysis of 293T (Human embryonic kidney epithelial cell, Left) / SH-SY5Y (Human neuroblastoma epithelial cell, Right) cells labelling GABA A Receptor gamma 2/GABRG2 with ab288564 at 1/50 dilution (1ug)/ Left and Right. A Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/2000 dilution was used as the secondary antibody. Negative control: 293T (PMID: 27864268). Gated on viable cells.

  • Immunoprecipitation - Anti-GABA A Receptor gamma 2/GABRG2 antibody [EPR25325-5] (ab288564)
    Immunoprecipitation - Anti-GABA A Receptor gamma 2/GABRG2 antibody [EPR25325-5] (ab288564)

    GABA A Receptor gamma 2/GABRG2 was immunoprecipitated from 0.35 mg Mouse brain tissue lysate 10ug with ab288564 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab288564 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution.

    Lane 1: Mouse brain tissue lysate 10ug

    Lane 2: ab288564 IP in Mouse brain tissue lysate

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab288564 in Mouse brain tissue lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 101 seconds

    Observed MW(KDa) 50

  • Anti-GABA A Receptor gamma 2/GABRG2 antibody [EPR25325] (ab288564)
    Anti-GABA A Receptor gamma 2/GABRG2 antibody [EPR25325] (ab288564)

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

References (0)

Publishing research using ab288564? Please let us know so that we can cite the reference in this datasheet.

ab288564 has not yet been referenced specifically in any publications.

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