Recombinant Anti-GABA Transporter 1 / GAT 1 antibody [EPR12998] (ab177483)
![Western blot - Anti-GABA Transporter 1 / GAT 1 antibody [EPR12998] (ab177483)](https://www.abcam.com/ps/products/177/ab177483/Images/ab177483-341537-ab177483.jpg "Western blot - Anti-GABA Transporter 1 / GAT 1 antibody [EPR12998] (ab177483)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR12998] to GABA Transporter 1 / GAT 1
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-GABA Transporter 1 / GAT 1 antibody [EPR12998]
See all GABA Transporter 1 / GAT 1 primary antibodies -
Description
Rabbit monoclonal [EPR12998] to GABA Transporter 1 / GAT 1 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details
Unsuitable for: ICC/IF,IHC-P or IP -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide within Human GABA Transporter 1/ GAT 1 aa 1-100 (Cysteine residue). The exact sequence is proprietary.
Database link: P30531 -
Positive control
- Fetal brain lysate.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.5% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR12998 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab177483 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/1000 - 1/10000. Predicted molecular weight: 67 kDa.
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| Notes |
|---|
|
WB
1/1000 - 1/10000. Predicted molecular weight: 67 kDa. |
Target
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Function
Terminates the action of GABA by its high affinity sodium-dependent reuptake into presynaptic terminals. -
Sequence similarities
Belongs to the sodium:neurotransmitter symporter (SNF) (TC 2.A.22) family. SLC6A1 subfamily. -
Domain
The PDZ domain-binding motif is involved in the interaction with MPP5. -
Cellular localization
Cell membrane. Membrane. Localized at the plasma membrane and in a subset of intracellular vesicles. Localized at the presynaptic terminals of interneurons. - Information by UniProt
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Database links
- Entrez Gene: 6529 Human
- Entrez Gene: 232333 Mouse
- Entrez Gene: 79212 Rat
- Omim: 137165 Human
- SwissProt: P30531 Human
- SwissProt: P31648 Mouse
- SwissProt: P23978 Rat
- Unigene: 443874 Human
see all -
Alternative names
- GABATHG antibody
- GABATR antibody
- GABT 1 antibody
see all
Images
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Western blot - Anti-GABA Transporter 1 / GAT 1 antibody [EPR12998] (ab177483)All lanes : Anti-GABA Transporter 1 / GAT 1 antibody [EPR12998] (ab177483) at 1/10000 dilution
Lane 1 : Human brain lysates unboiled
Lane 2 : Human brain lysates boiled
Lane 3 : Mouse brain unboiled
Lane 4 : Mouse brain boiled
Lane 5 : Rat brain unboiled
Lane 6 : Rat brain boiled
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 67 kDa
Observed band size: 67 kDaBlocking and dilution buffer: 5% NFDM/TBST.
Exposure time:
Lane 1 to 2: 20 seconds
Lane 3 to 6: 8 secondsWe recommend not to boil the samples after lysis to get desired WB results.
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![Western blot - Anti-GABA Transporter 1 / GAT 1 antibody [EPR12998] (ab177483)](https://www.abcam.com/ps/products/177/ab177483/Images/ab177483-205382-anti-gaba-transporter-1-gat-1-antibody-epr12998-western-blot.jpg)
Western blot - Anti-GABA Transporter 1 / GAT 1 antibody [EPR12998] (ab177483)Anti-GABA Transporter 1 / GAT 1 antibody [EPR12998] (ab177483) at 1/1000 dilution + Fetal brain lysate at 10 µg
Predicted band size: 67 kDa -
![Anti-GABA Transporter 1 / GAT 1 antibody [EPR12998] (ab177483)](https://www.abcam.com/ps/products/177/ab177483/Images/ab177483-3-benefits-of-recombinant-antibodies.png)
Anti-GABA Transporter 1 / GAT 1 antibody [EPR12998] (ab177483)
Datasheets and documents
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SDS download
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Datasheet download
References (4)
ab177483 has been referenced in 4 publications.
- Jin L et al. Astrocytic SARM1 promotes neuroinflammation and axonal demyelination in experimental autoimmune encephalomyelitis through inhibiting GDNF signaling. Cell Death Dis 13:759 (2022). PubMed: 36055989
- Cai HY et al. Adjusting vascular permeability, leukocyte infiltration, and microglial cell activation to rescue dopaminergic neurons in rodent models of Parkinson's disease. NPJ Parkinsons Dis 7:91 (2021). PubMed: 34625569
- Ren W et al. Slc6a13 deficiency promotes Th17 responses during intestinal bacterial infection. Mucosal Immunol N/A:N/A (2018). PubMed: 30523310
- Maolakuerban N et al. MiR-200c-3p inhibits cell migration and invasion of clear cell renal cell carcinoma via regulating SLC6A1. Cancer Biol Ther 19:282-291 (2018). PubMed: 29394133