Recombinant Anti-GABARAPL2/GATE-16 antibody [EP4808] (ab126607)
![Western blot - Anti-GABARAPL2/GATE-16 antibody [EP4808] (ab126607)](https://www.abcam.com/ps/products/126/ab126607/Images/ab126607-259089-anti-gabarapl2-gate-16-antibody-ep4808-western-blot.jpg "Western blot - Anti-GABARAPL2/GATE-16 antibody [EP4808] (ab126607)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP4808] to GABARAPL2/GATE-16
- Suitable for: WB
- Knockout validated
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-GABARAPL2/GATE-16 antibody [EP4808]
See all GABARAPL2/GATE-16 primary antibodies -
Description
Rabbit monoclonal [EP4808] to GABARAPL2/GATE-16 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details
Unsuitable for: Flow Cyt,ICC/IF,IHC-P or IP -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat
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Immunogen
Synthetic peptide within Human GABARAPL2/GATE-16 aa 50-150. The exact sequence is proprietary.
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Positive control
- HeLa, Human fetal brain, Human heart and Human spleen lysates.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. -
Dissociation constant (KD)
KD = 6.50 x 10 -11 M Learn more about KD -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 0.05% BSA, 40% Glycerol (glycerin, glycerine), 59% PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EP4808 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab126607 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | (4) |
1/1000 - 1/10000. Detects a band of approximately 14 kDa (predicted molecular weight: 14 kDa).
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| Notes |
|---|
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WB
1/1000 - 1/10000. Detects a band of approximately 14 kDa (predicted molecular weight: 14 kDa). |
Target
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Function
Involved in intra-Golgi traffic. Modulates intra-Golgi transport through coupling between NSF activity and SNAREs activation. It first stimulates the ATPase activity of NSF which in turn stimulates the association with GOSR1. -
Tissue specificity
Ubiquitous. Expressed at high levels in the brain, heart, prostate, ovary, spleen and skeletal muscle. Expressed at very low levels in lung, thymus and small intestine. -
Sequence similarities
Belongs to the MAP1 LC3 family. -
Post-translational
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR. -
Cellular localization
Golgi apparatus. - Information by UniProt
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Database links
- Entrez Gene: 11345 Human
- Entrez Gene: 93739 Mouse
- Entrez Gene: 100359937 Rat
- Entrez Gene: 64670 Rat
- Omim: 607452 Human
- SwissProt: P60520 Human
- SwissProt: P60521 Mouse
- SwissProt: P60522 Rat
see all -
Alternative names
- ATG8 antibody
- ATG8C antibody
- FLC3A antibody
see all
Images
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Western blot - Anti-GABARAPL2/GATE-16 antibody [EP4808] (ab126607)
Lane 1: Wild-type HAP1 cell lysate (20 µg)
Lane 2: GABARAPL2 / GATE-16 knockout HAP1 cell lysate (20 µg)
Lane 3: HeLa cell lysate (20 µg)
Lane 4: Human tonsil lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab126607 observed at 15 kDa. Red - loading control, ab8245, observed at 37 kDa.ab126607 was shown to specifically react with GABARAPL2 / GATE-16 when GABARAPL2 / GATE-16 knockout samples were used. Wild-type and GABARAPL2 / GATE-16 knockout samples were subjected to SDS-PAGE. ab126607 and ab8245 (loading control to GAPDH) were diluted 1/1000 and 1/10 000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10 000 dilution for 1 h at room temperature before imaging.
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![Western blot - Anti-GABARAPL2/GATE-16 antibody [EP4808] (ab126607)](https://www.abcam.com/ps/products/126/ab126607/Images/ab126607-140577-anti-gabarapl2-gate-16-antibody-ep4808-western-blot.jpg)
Western blot - Anti-GABARAPL2/GATE-16 antibody [EP4808] (ab126607)All lanes : Anti-GABARAPL2/GATE-16 antibody [EP4808] (ab126607) at 1/1000 dilution
Lane 1 : Human fetal brain tissue lysate
Lane 2 : Human heart tissue lysate
Lane 3 : HeLa cell lysate
Lane 4 : Human spleen tissue lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-Rabbit HRP at 1/2000 dilution
Predicted band size: 14 kDa
Observed band size: 14 kDa -
![OI-RD Scanning - Anti-GABARAPL2/GATE-16 antibody [EP4808] (ab126607)](https://www.abcam.com/ps/products/126/ab126607/Images/ab126607-140576-anti-gabarapl2-gate-16-antibody-ep4808.jpg)
OI-RD Scanning - Anti-GABARAPL2/GATE-16 antibody [EP4808] (ab126607) -
![Anti-GABARAPL2/GATE-16 antibody [EP4808] (ab126607)](https://www.abcam.com/ps/products/126/ab126607/Images/ab126607-4-benefits-of-recombinant-antibodies.png)
Anti-GABARAPL2/GATE-16 antibody [EP4808] (ab126607)
Datasheets and documents
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SDS download
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Datasheet download
References (5)
ab126607 has been referenced in 5 publications.
- Abdelmoaty MM et al. Monocyte biomarkers define sargramostim treatment outcomes for Parkinson's disease. Clin Transl Med 12:e958 (2022). PubMed: 35802825
- Agrotis A et al. Redundancy of human ATG4 protease isoforms in autophagy and LC3/GABARAP processing revealed in cells. Autophagy 15:976-997 (2019). PubMed: 30661429
- Holdgaard SG et al. Selective autophagy maintains centrosome integrity and accurate mitosis by turnover of centriolar satellites. Nat Commun 10:4176 (2019). PubMed: 31519908
- Chino H et al. Intrinsically Disordered Protein TEX264 Mediates ER-phagy. Mol Cell 74:909-921.e6 (2019). PubMed: 31006538
- Habisov S et al. Structural and Functional Analysis of a Novel Interaction Motif within UFM1-activating Enzyme 5 (UBA5) Required for Binding to Ubiquitin-like Proteins and Ufmylation. J Biol Chem 291:9025-41 (2016). PubMed: 26929408