Recombinant Anti-GABARAPL2/GATE-16 antibody [EP4808] (ab126607)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP4808] to GABARAPL2/GATE-16
- Suitable for: WB
- Knockout validated
- Reacts with: Human
Related conjugates and formulations
Overview
-
Product name
Anti-GABARAPL2/GATE-16 antibody [EP4808]
See all GABARAPL2/GATE-16 primary antibodies -
Description
Rabbit monoclonal [EP4808] to GABARAPL2/GATE-16 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details
Unsuitable for: Flow Cyt,ICC/IF,IHC-P or IP -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Synthetic peptide within Human GABARAPL2/GATE-16 aa 50-150. The exact sequence is proprietary.
-
Positive control
- HeLa, Human fetal brain, Human heart and Human spleen lysates.
-
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. -
Dissociation constant (KD)
KD = 6.50 x 10 -11 M Learn more about KD -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 0.05% BSA, 40% Glycerol (glycerin, glycerine), 59% PBS -
Concentration information loading...
-
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EP4808 -
Isotype
IgG -
Research areas
Associated products
-
Alternative Versions
-
Isotype control
-
Positive Controls
-
Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab126607 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB | (4) |
1/1000 - 1/10000. Detects a band of approximately 14 kDa (predicted molecular weight: 14 kDa).
|
Notes |
---|
WB
1/1000 - 1/10000. Detects a band of approximately 14 kDa (predicted molecular weight: 14 kDa). |
Target
-
Function
Involved in intra-Golgi traffic. Modulates intra-Golgi transport through coupling between NSF activity and SNAREs activation. It first stimulates the ATPase activity of NSF which in turn stimulates the association with GOSR1. -
Tissue specificity
Ubiquitous. Expressed at high levels in the brain, heart, prostate, ovary, spleen and skeletal muscle. Expressed at very low levels in lung, thymus and small intestine. -
Sequence similarities
Belongs to the MAP1 LC3 family. -
Post-translational
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR. -
Cellular localization
Golgi apparatus. - Information by UniProt
-
Database links
- Entrez Gene: 11345 Human
- Entrez Gene: 93739 Mouse
- Entrez Gene: 100359937 Rat
- Entrez Gene: 64670 Rat
- Omim: 607452 Human
- SwissProt: P60520 Human
- SwissProt: P60521 Mouse
- SwissProt: P60522 Rat
see all -
Alternative names
- ATG8 antibody
- ATG8C antibody
- FLC3A antibody
see all
Images
-
Lane 1: Wild-type HAP1 cell lysate (20 µg)
Lane 2: GABARAPL2 / GATE-16 knockout HAP1 cell lysate (20 µg)
Lane 3: HeLa cell lysate (20 µg)
Lane 4: Human tonsil lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab126607 observed at 15 kDa. Red - loading control, ab8245, observed at 37 kDa.ab126607 was shown to specifically react with GABARAPL2 / GATE-16 when GABARAPL2 / GATE-16 knockout samples were used. Wild-type and GABARAPL2 / GATE-16 knockout samples were subjected to SDS-PAGE. ab126607 and ab8245 (loading control to GAPDH) were diluted 1/1000 and 1/10 000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10 000 dilution for 1 h at room temperature before imaging.
-
All lanes : Anti-GABARAPL2/GATE-16 antibody [EP4808] (ab126607) at 1/1000 dilution
Lane 1 : Human fetal brain tissue lysate
Lane 2 : Human heart tissue lysate
Lane 3 : HeLa cell lysate
Lane 4 : Human spleen tissue lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-Rabbit HRP at 1/2000 dilution
Predicted band size: 14 kDa
Observed band size: 14 kDa
Datasheets and documents
-
SDS download
-
Datasheet download
References (5)
ab126607 has been referenced in 5 publications.
- Abdelmoaty MM et al. Monocyte biomarkers define sargramostim treatment outcomes for Parkinson's disease. Clin Transl Med 12:e958 (2022). PubMed: 35802825
- Agrotis A et al. Redundancy of human ATG4 protease isoforms in autophagy and LC3/GABARAP processing revealed in cells. Autophagy 15:976-997 (2019). PubMed: 30661429
- Holdgaard SG et al. Selective autophagy maintains centrosome integrity and accurate mitosis by turnover of centriolar satellites. Nat Commun 10:4176 (2019). PubMed: 31519908
- Chino H et al. Intrinsically Disordered Protein TEX264 Mediates ER-phagy. Mol Cell 74:909-921.e6 (2019). PubMed: 31006538
- Habisov S et al. Structural and Functional Analysis of a Novel Interaction Motif within UFM1-activating Enzyme 5 (UBA5) Required for Binding to Ubiquitin-like Proteins and Ufmylation. J Biol Chem 291:9025-41 (2016). PubMed: 26929408