Anti-GAD65 + GAD67 antibody (ab49832)
Key features and details
- Rabbit polyclonal to GAD65 + GAD67
- Suitable for: IHC-P
- Reacts with: Rat
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
- Long-term and scalable supply – powered by recombinant technology for fast production
- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-GAD65 + GAD67 antibody
See all GAD65 + GAD67 primary antibodies -
Description
Rabbit polyclonal to GAD65 + GAD67 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-Pmore details -
Species reactivity
Reacts with: Rat -
Immunogen
Synthetic peptide:
K-DIDFLIEEIERLGQDL
conjugated to KLH by a Glutaraldehyde linker, corresponding to C terminal amino acids 579-594 (with N-terminally added lysine) of Human GAD67. The sequence is identical in Human GAD 65 (amino acids 570-585). -
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
Storage buffer
pH: 7.40
Preservative: 0.097% Sodium azide
Constituent: 0.0268% PBS -
Concentration information loading... -
Purity
Ion Exchange Chromatography -
Purification notes
Whole antiserum is fractionated and then further purified by ion-exchange chromatography to provide the IgG fraction of antiserum that is essentially free of other Rabbit serum proteins. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab49832 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | (2) |
1/1000 - 1/2000.
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| Notes |
|---|
|
IHC-P
1/1000 - 1/2000. |
Target
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Relevance
This gene encodes one of several forms of glutamic acid decarboxylase, identified as a major autoantigen in insulin-dependent diabetes. The enzyme encoded is responsible for catalyzing the production of gamma aminobutyric acid from L glutamic acid. A pathogenic role for this enzyme has been identified in the human pancreas since it has been identified as an autoantibody and an autoreactive T cell target in insulin dependent diabetes. This gene may also play a role in the stiff man syndrome. -
Cellular localization
Cytoplasm; cytosol. Associated to cytoplasmic vesicles. In neurons, cytosolic leaflet of Golgi membranes and presynaptic clusters. -
Database links
- Entrez Gene: 24379 Rat
- Entrez Gene: 24380 Rat
- SwissProt: P18088 Rat
- SwissProt: Q05683 Rat
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Alternative names
- 65 kDa glutamic acid decarboxylase antibody
- 67 kDa glutamic acid decarboxylase antibody
- GAD 65 antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GAD65 + GAD67 antibody (ab49832)
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Rat pancreatics sections labelling GAD65 & GAD67 with ab49832 at a concentration of 1/1000. The secondary used was a biotin peroxidase.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GAD65 + GAD67 antibody (ab49832)This image is courtesy of an anonymous Abreviewab49832 staining human pancreas tissue sections by IHC-P. Sections were formaldehyde fixed and subjected to heat mediated antigen retreival in 10mM citrate buffer prior to incubation with ab49832, diluted 1/2000, for 1 hour at 25°C. An Alexa Fluor® 546 conjugated goat anti-rabbit antibody, diluted 1/200, was used as the secondary.
Datasheets and documents
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SDS download
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Datasheet download
References (29)
ab49832 has been referenced in 29 publications.
- Giehrl-Schwab J et al. Parkinson's disease motor symptoms rescue by CRISPRa-reprogramming astrocytes into GABAergic neurons. EMBO Mol Med 14:e14797 (2022). PubMed: 35373464
- Favrais G et al. Partial protective effects of melatonin on developing brain in a rat model of chorioamnionitis. Sci Rep 11:22167 (2021). PubMed: 34773065
- Grippo RM et al. Dopamine Signaling in the Suprachiasmatic Nucleus Enables Weight Gain Associated with Hedonic Feeding. Curr Biol 30:196-208.e8 (2020). PubMed: 31902720
- Chang W et al. PTH hypersecretion triggered by a GABAB1 and Ca2+-sensing receptor heterocomplex in hyperparathyroidism. Nat Metab 2:243-255 (2020). PubMed: 32694772
- Meyer G et al. Cajal-Retzius neurons are required for the development of the human hippocampal fissure. J Anat 235:569-589 (2019). PubMed: 30861578