Anti-GADD45G/CR6 antibody (ab196774)
Key features and details
- Rabbit polyclonal to GADD45G/CR6
- Suitable for: ICC/IF, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-GADD45G/CR6 antibody -
Description
Rabbit polyclonal to GADD45G/CR6 -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse
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Immunogen
Synthetic peptide corresponding to Human GADD45G/CR6 (internal sequence).
Database link: O95257 -
Positive control
- A549 cells, Human lung carcinoma tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, 49% PBS
PBS is without Mg2+ and Ca2+. -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab196774 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab196774 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF |
1/100 - 1/500.
|
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| IHC-P | (1) |
1/50 - 1/100.
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| Notes |
|---|
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ICC/IF
1/100 - 1/500. |
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IHC-P
1/50 - 1/100. |
Target
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Function
Involved in the regulation of growth and apoptosis. Mediates activation of stress-responsive MTK1/MEKK4 MAPKKK. -
Sequence similarities
Belongs to the GADD45 family. - Information by UniProt
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Database links
- Entrez Gene: 10912 Human
- Entrez Gene: 23882 Mouse
- Omim: 604949 Human
- SwissProt: O95257 Human
- SwissProt: Q9Z111 Mouse
- Unigene: 9701 Human
- Unigene: 281298 Mouse
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Alternative names
- CR6 antibody
- Cytokine responsive protein CR6 antibody
- Cytokine-responsive protein CR6 antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GADD45G/CR6 antibody (ab196774)
Immunohistochemical analysis of paraffin-embedded Human lung carcinoma tissue labeling GADD45G/CR6 with ab196774 at 1/50 dilution. The image on the right is treated with synthesized peptide.
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Immunocytochemistry/ Immunofluorescence - Anti-GADD45G/CR6 antibody (ab196774)Immunofluorescent analysis of A549 cells labelling GADD45G/CR6 with ab196774 at a 1/00 dilution. The image on the right is treated with synthesized peptide.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (4)
ab196774 has been referenced in 4 publications.
- Janisiak J et al. Protein Arginine Methyltransferase (PRMT) Inhibitors-AMI-1 and SAH Are Effective in Attenuating Rhabdomyosarcoma Growth and Proliferation in Cell Cultures. Int J Mol Sci 22:N/A (2021). PubMed: 34360791
- Zhou L et al. GADD45a Promotes Active DNA Demethylation of the MMP-9 Promoter via Base Excision Repair Pathway in AGEs-Treated Keratinocytes and in Diabetic Male Rat Skin. Endocrinology 159:1172-1186 (2018). PubMed: 29244109
- Sarmento-Cabral A et al. Metformin Reduces Prostate Tumor Growth, in a Diet-Dependent Manner, by Modulating Multiple Signaling Pathways. Mol Cancer Res 15:862-874 (2017). PubMed: 28385910
- Su X et al. IL-27 attenuates airway inflammation in a mouse asthma model via the STAT1 and GADD45?/p38 MAPK pathways. J Transl Med 14:283 (2016). WB ; Mouse, Human . PubMed: 27687913