Anti-GAL4 antibody [15-6E10A7] (ab135397)
![Western blot - Anti-GAL4 antibody [15-6E10A7] (ab135397)](https://www.abcam.com/ps/products/135/ab135397/Images/ab135397-149613-anti-gal4-antibody-15-6e10a7-western-blot.jpg "Western blot - Anti-GAL4 antibody [15-6E10A7] (ab135397)")
Key features and details
- Mouse monoclonal [15-6E10A7] to GAL4
- Suitable for: WB
- Reacts with: Saccharomyces cerevisiae
- Isotype: IgG1
Overview
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Product name
Anti-GAL4 antibody [15-6E10A7]
See all GAL4 primary antibodies -
Description
Mouse monoclonal [15-6E10A7] to GAL4 -
Host species
Mouse -
Specificity
This antibody binds specifically to the DNA binding domain of the yeast GAL4 protein.
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Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Saccharomyces cerevisiae -
Immunogen
Recombinant fragment. This information is considered to be commercially sensitive.
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General notes
The GAL4 DNA-BD monoclonal antibody binds specifically to the DNA-binding domain of the yeast GAL4 protein. This antibody is useful for verifying the expression and correct molecular weight of bait fusion proteins containing GAL4 DNA BD as the fusion partner in yeast two hybrid studies. The binding domain is typically used in a two-hybrid experiment as the fusion partner for the protein of interest. When the fusion protein of interest cannot activate transcription on its own, it can be used as bait to screen a library of cDNA clones that are fused to an activation domain. GAL4 is a positive regulator for the gene expression of the galactose-induced genes such as GAL1, GAL2, GAL7, GAL10, and MEL1 which encode for the enzymes used to convert galactose to glucose. It recognizes a 17 base pair sequence in (5'-CGGRNNRCYNYNCNCCG-3') the upstream activating sequence (UAS-G) of these genes. Subunit structure: Binds DNA as a homodimer. Interacts directly with the mediator subunits GAL11/MED15 and SRB4/MED17. Domain: The 9aaTAD motif (residues 862 to 870) is a transactivation domain present in a large number of yeast and animal transcription factors. Post-translational modification: Association between GAL11 and GAL4 may serve to expedite phosphorylation of GAL4.
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Product was previously marketed under the MitoSciences sub-brand.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7.5
Preservative: 0.02% Sodium azide -
Concentration information loading... -
Purity
Ammonium Sulphate Precipitation -
Primary antibody notes
The GAL4 DNA-BD monoclonal antibody binds specifically to the DNA-binding domain of the yeast GAL4 protein. This antibody is useful for verifying the expression and correct molecular weight of bait fusion proteins containing GAL4 DNA BD as the fusion partner in yeast two hybrid studies. The binding domain is typically used in a two-hybrid experiment as the fusion partner for the protein of interest. When the fusion protein of interest cannot activate transcription on its own, it can be used as bait to screen a library of cDNA clones that are fused to an activation domain. GAL4 is a positive regulator for the gene expression of the galactose-induced genes such as GAL1, GAL2, GAL7, GAL10, and MEL1 which encode for the enzymes used to convert galactose to glucose. It recognizes a 17 base pair sequence in (5'-CGGRNNRCYNYNCNCCG-3') the upstream activating sequence (UAS-G) of these genes. Subunit structure: Binds DNA as a homodimer. Interacts directly with the mediator subunits GAL11/MED15 and SRB4/MED17. Domain: The 9aaTAD motif (residues 862 to 870) is a transactivation domain present in a large number of yeast and animal transcription factors. Post-translational modification: Association between GAL11 and GAL4 may serve to expedite phosphorylation of GAL4. -
Clonality
Monoclonal -
Clone number
15-6E10A7 -
Isotype
IgG1 -
Research areas
Associated products
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Compatible Secondaries
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Corresponding Antibody
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab135397 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
Use a concentration of 1 µg/ml. Predicted molecular weight: 99 kDa.
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| Notes |
|---|
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WB
Use a concentration of 1 µg/ml. Predicted molecular weight: 99 kDa. |
Target
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Relevance
Function: This protein is a positive regulator for the gene expression of the galactose-induced genes such as GAL1, GAL2, GAL7, GAL10, and MEL1 which encode for the enzymes used to convert galactose to glucose. It recognizes a 17 base pair sequence in (5'-CGGRNNRCYNYNCNCCG-3') the upstream activating sequence (UAS-G) of these genes. Subunit structure: Binds DNA as a homodimer. Interacts directly with the mediator subunits GAL11/MED15 and SRB4/MED17. Domain: The 9aaTAD motif (residues 862 to 870) is a transactivation domain present in a large number of yeast and animal transcription factors. Post-translational modification: Association between GAL11 and GAL4 may serve to expedite phosphorylation of GAL4. -
Cellular localization
Nuclear -
Database links
- Entrez Gene: 855828 Saccharomyces cerevisiae
- SwissProt: P04386 Saccharomyces cerevisiae
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Alternative names
- Gal4p antibody
- GAL81 antibody
- Regulatory protein GAL4 antibody
Images
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Western blot - Anti-GAL4 antibody [15-6E10A7] (ab135397)All lanes : Anti-GAL4 antibody [15-6E10A7] (ab135397) at 1 µg/ml
Lane 1 : Whole cell yeast lysate control from untransformed SEY6210 pep4-3 strain (a kind gift from Prof. Tom Stevens, University of Oregon).
Lane 2 : Whole cell yeast lysate from SEY6210 pep4-3 strain transformed with pJK22-pGBDU plasmid containing GAL4 DNA Binding domain fused to the VPS60 Gene (a kind gift from Prof. Tom Stevens, University of Oregon).
Lane 3 : Whole cell yeast lysate from SEY6210 pep4-3 strain transformed with pJk23-pGAD plasmid containing GAL4 Active Domain fused to the VPS60 Gene (a kind gift from Prof. Tom Stevens, University of Oregon).
Secondary
All lanes : Anti-Mouse IgG (H&L) Goat Polyclonal
Performed under reducing conditions.
Predicted band size: 99 kDa
Additional bands at: 56 kDa (possible isoform), 56 kDa (possible Fusion protein)
Exposure time: 3 minutes
Datasheets and documents
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Datasheet download
References (3)
ab135397 has been referenced in 3 publications.
- Zhai K et al. RRM Transcription Factors Interact with NLRs and Regulate Broad-Spectrum Blast Resistance in Rice. Mol Cell 74:996-1009.e7 (2019). PubMed: 30975460
- Rother S et al. NF-?B-repressing factor phosphorylation regulates transcription elongation via its interactions with 5'?3' exoribonuclease 2 and negative elongation factor. FASEB J 30:174-85 (2016). WB . PubMed: 26340924
- Geng S et al. Protein Interaction between Ameloblastin and Proteasome Subunit a Type 3 Can Facilitate Redistribution of Ameloblastin Domains within Forming Enamel. J Biol Chem 290:20661-73 (2015). PubMed: 26070558
