Recombinant Anti-GALR2 antibody [EPR24328A-120] (ab271019)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GALR2 antibody [EPR24328A-120] (ab271019)](https://www.abcam.com/ps/products/271/ab271019/Images/ab271019-1-anti-galr2-antibody-epr24328120-immunohistochemistry-rat-dorsal-root-ganglia.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GALR2 antibody [EPR24328A-120] (ab271019)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR24328A-120] to GALR2
- Suitable for: IHC-Fr, IHC-P, ICC/IF
- Reacts with: Rat
Related conjugates and formulations
Overview
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Product name
Anti-GALR2 antibody [EPR24328A-120]
See all GALR2 primary antibodies -
Description
Rabbit monoclonal [EPR24328A-120] to GALR2 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-Fr, IHC-P, ICC/IFmore details
Unsuitable for: Flow Cyt,IP or WB -
Species reactivity
Reacts with: Rat -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- IHC-P: Rat dorsal root ganglia, Rat cerebrum tissue. IHC-Fr: Rat hypothalamus, Rat cerebral cortex tissue. ICC/IF: Rat primary neuron cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59.94% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR24328A-120 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab271019 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-Fr |
1/100.
Perform heat mediated antigen retrieval with sodium citrate buffer at pH 6.0 before commencing with IHC staining protocol. |
|
| IHC-P |
1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
|
|
| ICC/IF |
1/100.
|
| Notes |
|---|
|
IHC-Fr
1/100. Perform heat mediated antigen retrieval with sodium citrate buffer at pH 6.0 before commencing with IHC staining protocol. |
|
IHC-P
1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
|
ICC/IF
1/100. |
Target
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Function
Receptor for the hormone galanin and for GALP. The activity of this receptor is mediated by G proteins that activate the phospholipase C/protein kinase C pathway (via G(q)) and that inhibit adenylyl cyclase (via G(i)). -
Tissue specificity
Expressed abundantly within the central nervous system in both hypothalamus and hippocampus. In peripheral tissues, the strongest expression was observed in heart, kidney, liver, and small intestine. -
Sequence similarities
Belongs to the G-protein coupled receptor 1 family. -
Cellular localization
Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 29234 Rat
- SwissProt: O08726 Rat
- Unigene: 9789 Rat
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Alternative names
- GAL2-R antibody
- GAL2R antibody
- Galanin receptor 2 antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GALR2 antibody [EPR24328A-120] (ab271019)
Immunohistochemical analysis of paraffin-embedded Rat dorsal root ganglia tissue labelling GALR2 with ab271019 at 1/2000 (0.259 ug/ml) dilution followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer). Cytoplasmic staining in rat dorsal root ganglia.The section was incubated with ab271019 at 4oC overnight. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer).
Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0)
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GALR2 antibody [EPR24328A-120] (ab271019)](https://www.abcam.com/ps/products/271/ab271019/Images/ab271019-2-anti-galr2-antibody-epr24328120-immunohistochemistry-rat-cerebrum.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GALR2 antibody [EPR24328A-120] (ab271019)Immunohistochemical analysis of paraffin-embedded Rat cerebrum tissue labelling GALR2 with ab271019 at 1/2000 (0.259 ug/ml) dilution followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer). Cytoplasmic staining in rat cerebrum. The section was incubated with ab271019 at 4oC overnight. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer).
Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0)
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![Immunohistochemistry (Frozen sections) - Anti-GALR2 antibody [EPR24328A-120] (ab271019)](https://www.abcam.com/ps/products/271/ab271019/Images/ab271019-4-anti-galr2-antibody-epr24328120-immunohistochemistry-frozen-rat-hypothalamus.jpg)
Immunohistochemistry (Frozen sections) - Anti-GALR2 antibody [EPR24328A-120] (ab271019)Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Rat hypothalamus tissue labeling GALR2 with ab271019 at 1/100 (5.17 ug/ml) dilution followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (Green). Cytoplasmic staining on rat hypothalamus is observed. The nuclear counterstain was DAPI (Blue).
Secondary antibody control: Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution.
Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).
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![Immunohistochemistry (Frozen sections) - Anti-GALR2 antibody [EPR24328A-120] (ab271019)](https://www.abcam.com/ps/products/271/ab271019/Images/ab271019-5-anti-galr2-antibody-epr24328120-immunohistochemistry-frozen-rat-cerebral-cortex.jpg)
Immunohistochemistry (Frozen sections) - Anti-GALR2 antibody [EPR24328A-120] (ab271019)Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Rat cerebral cortex tissue labeling GALR2 with ab271019 at 1/100 (5.17 ug/ml) dilution followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (Green). Cytoplasmic staining on rat cerebral cortex is observed. The nuclear counterstain was DAPI (Blue).
Secondary antibody control: Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution.
Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).
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![Immunocytochemistry/ Immunofluorescence - Anti-GALR2 antibody [EPR24328A-120] (ab271019)](https://www.abcam.com/ps/products/271/ab271019/Images/ab271019-3-anti-galr2-antibody-epr24328120-immunocytochemistry-rat-primary-neuron.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-GALR2 antibody [EPR24328A-120] (ab271019)Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized rat primary neuron cells labelling GALR2 with ab271019 at 1/100 (5.17 ug/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2 ug/ml dilution (Green) Confocal image showing cytoplasmic staining in rat primary neuron. Confocal scanning Z step was set as 0.3 µm followed by image processing with maximum Z projection. is observed. ab11267 Anti-MAP2 mouse monoclonal antibody was used to counterstain tubulin at 1/500 4ug/ml dilution (Red). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/ml) dilution.
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![Anti-GALR2 antibody [EPR24328A-120] (ab271019)](https://www.abcam.com/ps/products/271/ab271019/Images/ab271019-6-Benefits-of-Recombinant-antibodies.jpg)
Anti-GALR2 antibody [EPR24328A-120] (ab271019)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (0)
ab271019 has not yet been referenced specifically in any publications.