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    products/primary-antibodies/gap43-antibody-ep890y-neuronal-marker-ab75810.pdf

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RecombinantRabMAb

Recombinant Anti-GAP43 antibody [EP890Y] - Neuronal Marker (ab75810)

  • Datasheet
  • SDS
Reviews (8) Submit a question References (54)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GAP43 antibody [EP890Y] - Neuronal Marker (ab75810)
  • Western blot - Anti-GAP43 antibody [EP890Y] - Neuronal Marker (ab75810)
  • Immunocytochemistry/ Immunofluorescence - Anti-GAP43 antibody [EP890Y] - Neuronal Marker (ab75810)
  • Immunoprecipitation - Anti-GAP43 antibody [EP890Y] - Neuronal Marker (ab75810)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GAP43 antibody [EP890Y] - Neuronal Marker (ab75810)
  • Flow Cytometry (Intracellular) - Anti-GAP43 antibody [EP890Y] - Neuronal Marker (ab75810)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GAP43 antibody [EP890Y] - Neuronal Marker (ab75810)
  • Western blot - Anti-GAP43 antibody [EP890Y] - Neuronal Marker (ab75810)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GAP43 antibody [EP890Y] - Neuronal Marker (ab75810)
  • Flow Cytometry (Intracellular) - Anti-GAP43 antibody [EP890Y] - Neuronal Marker (ab75810)
  • Immunocytochemistry/ Immunofluorescence - Anti-GAP43 antibody [EP890Y] - Neuronal Marker (ab75810)
  • Anti-GAP43 antibody [EP890Y] - Neuronal Marker (ab75810)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP890Y] to GAP43 - Neuronal Marker
  • Suitable for: Flow Cyt (Intra), ICC/IF, WB, IP, IHC-P
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Alexa Fluor® 488 Alexa Fluor® 647 Carrier Free HRP PE

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Recombinant Human GAP43 protein (ab114188)
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Anti-MAP2 antibody [EPR19691] (ab183830)
Conjugation
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DyLight® 633 Conjugation Kit (Fast) - Lightning-Link® (ab201802)

View more associated products

Overview

  • Product name

    Anti-GAP43 antibody [EP890Y] - Neuronal Marker
    See all GAP43 primary antibodies
  • Description

    Rabbit monoclonal [EP890Y] to GAP43 - Neuronal Marker
  • Host species

    Rabbit
  • Tested applications

    Suitable for: Flow Cyt (Intra), ICC/IF, WB, IP, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: SH-SY5Y, Human brain, Mouse brain, Rat brain, and PC-12 lysates. IHC-P: human cerebrum, mouse cerebrum, and rat cerebrum tissues. ICC/IF: Neuro-2a cells. Flow Cyt (intra): SH-SY5Y cells. IP: SH-SY5Y cells.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EP890Y
  • Isotype

    IgG
  • Research areas

    • Neuroscience
    • Cell Type Marker
    • Neuron marker
    • Growth Cone
    • Neuroscience
    • Cell Type Marker
    • Neuron marker
    • Synapse marker
    • Neuroscience
    • Neurology process
    • Growth and Development
    • Axonal Guidance Proteins
    • Neuroscience
    • Development

Associated products

  • Alternative Versions

    • Alexa Fluor® 488 Anti-GAP43 antibody [EP890Y] - Neuronal Marker (ab196324)
    • HRP Anti-GAP43 antibody [EP890Y] - Neuronal Marker (ab196325)
    • Alexa Fluor® 647 Anti-GAP43 antibody [EP890Y] - Neuronal Marker (ab196540)
    • PE Anti-GAP43 antibody [EP890Y] - Neuronal Marker (ab208745)
    • Anti-GAP43 antibody [EP890Y] - BSA and Azide free (ab219582)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Goat Anti-Rabbit IgG H&L (DyLight® 488) preadsorbed (ab96899)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Recombinant Protein

    • Recombinant Human GAP43 protein (ab114188)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab75810 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt (Intra)
1/20.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

ICC/IF (1)
1/160.

For unpurified use at 1/500.

 

WB (2)
1/1000. Detects a band of approximately 48 kDa (predicted molecular weight: 25 kDa).

For unpurified use at 1/100000 - 1/200000.

The expression of GAP43 is undetectable in undifferentiated PC-12 cells in Western Blot (Ref: PMID: 2139463, PMID: 15969743)

IP
1/20 - 1/50.
IHC-P (5)
1/3000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

See IHC antigen retrieval protocols.

For unpurified use at 1/500.

Notes
Flow Cyt (Intra)
1/20.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

ICC/IF
1/160.

For unpurified use at 1/500.

 

WB
1/1000. Detects a band of approximately 48 kDa (predicted molecular weight: 25 kDa).

For unpurified use at 1/100000 - 1/200000.

The expression of GAP43 is undetectable in undifferentiated PC-12 cells in Western Blot (Ref: PMID: 2139463, PMID: 15969743)

IP
1/20 - 1/50.
IHC-P
1/3000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

See IHC antigen retrieval protocols.

For unpurified use at 1/500.

Target

  • Function

    This protein is associated with nerve growth. It is a major component of the motile "growth cones" that form the tips of elongating axons.
  • Sequence similarities

    Belongs to the neuromodulin family.
    Contains 1 IQ domain.
  • Post-translational
    modifications

    Phosphorylation of this protein by a protein kinase C is specifically correlated with certain forms of synaptic plasticity.
  • Cellular localization

    Cell membrane. Cell projection > growth cone membrane. Cell junction > synapse. Cytoplasmic surface of growth cone and synaptic plasma membranes.
  • Target information above from: UniProt accession P17677 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 2596 Human
    • Entrez Gene: 14432 Mouse
    • Entrez Gene: 29423 Rat
    • GenBank: NP_002036 Human
    • Omim: 162060 Human
    • SwissProt: P17677 Human
    • SwissProt: P06837 Mouse
    • SwissProt: P07936 Rat
    • Unigene: 134974 Human
    • Unigene: 1222 Mouse
    • Unigene: 10928 Rat
    see all
  • Alternative names

    • Axonal membrane protein GAP 43 antibody
    • Axonal membrane protein GAP-43 antibody
    • B 50 antibody
    • Calmodulin binding protein P 57 antibody
    • F1 antibody
    • GAP 43 antibody
    • GAP43 antibody
    • Growth Associated Protein 43 antibody
    • Growth-associated protein 43 antibody
    • Nerve Growth Related Peptide antibody
    • Nerve growth related peptide GAP43 antibody
    • NEUM_HUMAN antibody
    • Neural phosphoprotein B 50 antibody
    • Neural phosphoprotein B-50 antibody
    • Neuromodulin antibody
    • Neuron growth associated protein 43 antibody
    • PP46 antibody
    • Protein F1 antibody
    • QtrA-11580 antibody
    • QtrA-13071 antibody
    see all

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GAP43 antibody [EP890Y] - Neuronal Marker (ab75810)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GAP43 antibody [EP890Y] - Neuronal Marker (ab75810)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat cerebrum tissue sections labeling GAP43 with Purified ab75810 at 1:3000 dilution (0.07 µg/ml). Heat mediated antigen retrieval using Bond© Epitope Retrieval Solution 2 (pH 9.0). Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used for detection. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

  • Western blot - Anti-GAP43 antibody [EP890Y] - Neuronal Marker (ab75810)
    Western blot - Anti-GAP43 antibody [EP890Y] - Neuronal Marker (ab75810)
    All lanes : Anti-GAP43 antibody [EP890Y] - Neuronal Marker (ab75810) (Purified)

    Lane 1 : Human brain lysates
    Lane 2 : Mouse brain lysates
    Lane 4 : PC-12 (Rat adrenal gland pheochromocytoma) whole cell lysates

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 25 kDa
    Observed band size: 48 kDa why is the actual band size different from the predicted?



    This antibody fails to detect GAP43 in PC-12 cells which is positve as described in PMID: 21695168 

  • Immunocytochemistry/ Immunofluorescence - Anti-GAP43 antibody [EP890Y] - Neuronal Marker (ab75810)
    Immunocytochemistry/ Immunofluorescence - Anti-GAP43 antibody [EP890Y] - Neuronal Marker (ab75810)
    Immunocytochemistry/ Immunofluorescence analysis of Neuro-2a (Mouse neuroblastoma neuroblast) cells labeling GAP43 with Purified ab75810 at 1:160 dilution (1.4 µg/ml). Cells were fixed in 100% Methanol and permeabilized with None. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
  • Immunoprecipitation - Anti-GAP43 antibody [EP890Y] - Neuronal Marker (ab75810)
    Immunoprecipitation - Anti-GAP43 antibody [EP890Y] - Neuronal Marker (ab75810)
    ab75810 (Purified) at 1:20 dilution (1 µg) immunoprecipitating GAP43 in SH-SY5Y whole cell lysate.
    Lane 1 (input): SH-SY5Y (Human neuroblastoma epithelial cell) whole cell lysate 10 µg
    Lane 2 (+): ab75810 & SH-SY5Y whole cell lysate
    Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab75810 in SH-SY5Y whole cell lysate
    For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1:1000 dilution.
    Blocking and diluting buffer: 5% NFDM/TBST.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GAP43 antibody [EP890Y] - Neuronal Marker (ab75810)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GAP43 antibody [EP890Y] - Neuronal Marker (ab75810)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse cerebrum tissue sections labeling GAP43 with Purified ab75810 at 1:3000 dilution (0.07 µg/ml). Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0). Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used for detection. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
  • Flow Cytometry (Intracellular) - Anti-GAP43 antibody [EP890Y] - Neuronal Marker (ab75810)
    Flow Cytometry (Intracellular) - Anti-GAP43 antibody [EP890Y] - Neuronal Marker (ab75810)

    Intracellular Flow Cytometry analysis of SH-SY5Y (Human neuroblastoma epithelial cell) cells labeling GAP43 with Purified ab75810 at 1/20 dilution (10 µg/ml) (Red). Cells were fixed with 80% Methanol and permeabilised with 0.1% Tween-20. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).

     

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GAP43 antibody [EP890Y] - Neuronal Marker (ab75810)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GAP43 antibody [EP890Y] - Neuronal Marker (ab75810)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human cerebrum tissue sections labeling GAP43 with Purified ab75810 at 1:3000 dilution (0.07 µg/ml). Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0). Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used for detection. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
  • Western blot - Anti-GAP43 antibody [EP890Y] - Neuronal Marker (ab75810)
    Western blot - Anti-GAP43 antibody [EP890Y] - Neuronal Marker (ab75810)
    Anti-GAP43 antibody [EP890Y] - Neuronal Marker (ab75810) at 1/50000 dilution (Purified) + SH-SY5Y (Human neuroblastoma epithelial cell) whole cell lysates at 15 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 25 kDa
    Observed band size: 48 kDa why is the actual band size different from the predicted?

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GAP43 antibody [EP890Y] - Neuronal Marker (ab75810)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GAP43 antibody [EP890Y] - Neuronal Marker (ab75810)This image is a courtesy of anonymous Abreview.

    ab75810 (unpurified) staining GAP43 in Mouse ear tissue sections by Immunohistochemistry (Formalin/ PFA-fixed paraffin-embedded tissue sections).  The sections were formaldehyde fixed, subjected to heat mediated antigen retrieval at pH 6 and blocked for 10 minutes at 25C. The primary antibody was diluted 1/500 and incubated with the sample for 1 hour at 25°C. An HRP polymer anti-rabbit IgG system was used undiluted, as the secondary antibody.

    See Abreview

  • Flow Cytometry (Intracellular) - Anti-GAP43 antibody [EP890Y] - Neuronal Marker (ab75810)
    Flow Cytometry (Intracellular) - Anti-GAP43 antibody [EP890Y] - Neuronal Marker (ab75810)

    Overlay histogram showing SH-SY5Y cells stained with ab75810 (unpurified) (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab75810, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in SH-SY5Y cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions. 

     

     

  • Immunocytochemistry/ Immunofluorescence - Anti-GAP43 antibody [EP890Y] - Neuronal Marker (ab75810)
    Immunocytochemistry/ Immunofluorescence - Anti-GAP43 antibody [EP890Y] - Neuronal Marker (ab75810)

    ICC/IF image of ab75810 (unpurified) stained SKNSH cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab75810, 1/50 dilution) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG(H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Anti-GAP43 antibody [EP890Y] - Neuronal Marker (ab75810)
    Anti-GAP43 antibody [EP890Y] - Neuronal Marker (ab75810)

Protocols

  • Flow cytometry protocols
  • Immunoprecipitation protocols
  • Immunohistochemistry protocols
  • Immunocytochemistry & immunofluorescence protocols
  • Western blot protocols

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (54)

Publishing research using ab75810? Please let us know so that we can cite the reference in this datasheet.

ab75810 has been referenced in 54 publications.

  • Feng S  et al. Effects of combination treatment with transcranial magnetic stimulation and bone marrow mesenchymal stem cell transplantation or Raf inhibition on spinal cord injury in rats. Mol Med Rep 23:N/A (2021). PubMed: 33649786
  • Zhu S  et al. AAV2-mediated and hypoxia response element-directed expression of bFGF in neural stem cells showed therapeutic effects on spinal cord injury in rats. Cell Death Dis 12:274 (2021). PubMed: 33723238
  • Ferdoushi A  et al. Tumor innervation and clinical outcome in pancreatic cancer. Sci Rep 11:7390 (2021). PubMed: 33795769
  • Nazir FH  et al. Molecular forms of neurogranin in cerebrospinal fluid. J Neurochem 157:816-833 (2021). PubMed: 33249594
  • Kim HN  et al. A Western diet impairs CNS energy homeostasis and recovery after spinal cord injury: Link to astrocyte metabolism. Neurobiol Dis 141:104934 (2020). PubMed: 32376475
View all Publications for this product

Customer reviews and Q&As

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1-8 of 8 Abreviews or Q&A

Western blot abreview for Anti-GAP43 antibody [EP890Y] - Neuronal Marker

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - other (Brain tumor)
Gel Running Conditions
Reduced Denaturing (4-12%)
Loading amount
10 µg
Specification
Brain tumor
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: RT°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

DR. Ivano Mocavini

Verified customer

Submitted Feb 05 2021

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-GAP43 antibody [EP890Y] - Neuronal Marker

Good
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (brain (cortex))
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Tris/EDTA pH 9.0
Permeabilization
No
Specification
brain (cortex)
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Fixative
Paraformaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Nov 02 2020

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-GAP43 antibody [EP890Y] - Neuronal Marker

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (brain (cortex))
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Tris/EDTA pH 9.0
Permeabilization
No
Specification
brain (cortex)
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Fixative
Paraformaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Nov 02 2020

Immunocytochemistry/ Immunofluorescence abreview for Anti-GAP43 antibody [EP890Y] - Neuronal Marker

Excellent
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Monodelphis domestica Cell (Cortex)
Permeabilization
Yes - 0,1% Triton X-100 in PBS
Specification
Cortex
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 0.5% · Temperature: 22°C
Fixative
Paraformaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Jelena Ban

Verified customer

Submitted May 29 2019

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-GAP43 antibody [EP890Y] - Neuronal Marker

Good
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rat Tissue sections (Ear)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: pH 6.0 Citrate 70C 2hr
Permeabilization
No
Specification
Ear
Blocking step
PB ab64226 as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: RT°C
Fixative
Formaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Dec 04 2017

Western blot abreview for Anti-GAP43 antibody [EP890Y] - Neuronal Marker

Good
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (SH-SY5Y)
Gel Running Conditions
Reduced Denaturing
Loading amount
25 µg
Specification
SH-SY5Y
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Feb 09 2015

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-GAP43 antibody [EP890Y] - Neuronal Marker

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rat Tissue sections (DRG)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: sodium citrate ph6.0
Permeabilization
No
Specification
DRG
Blocking step
Serum as blocking agent for 10 minute(s) · Concentration: 5% · Temperature: 37°C
Fixative
Paraformaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Mr. YONGGANG ZHAO

Verified customer

Submitted Mar 16 2012

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-GAP43 antibody [EP890Y] - Neuronal Marker

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Mouse ear)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Dako K8005 Citrate pH6
Specification
Mouse ear
Blocking step
DEEB DAKO S2003 as blocking agent for 10 minute(s) · Concentration: 100% · Temperature: RT°C
Fixative
Formaldehyde
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The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

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Verified customer

Submitted May 14 2010

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