Anti-GAPDH antibody [3E8AD9] - Loading Control (ab110305)
![Western blot - Anti-GAPDH antibody [3E8AD9] - Loading Control (ab110305)](https://www.abcam.com/ps/products/110/ab110305/Images/ab110305-123569-anti-gapdh-antibody-3e8ad9-loading-control-western-blot.gif "Western blot - Anti-GAPDH antibody [3E8AD9] - Loading Control (ab110305)")
Key features and details
- Mouse monoclonal [3E8AD9] to GAPDH - Loading Control
- Suitable for: ICC/IF, WB, Flow Cyt, IP
- Reacts with: Human
- Isotype: IgG2b
Related conjugates and formulations
Overview
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Product name
Anti-GAPDH antibody [3E8AD9] - Loading Control
See all GAPDH primary antibodies -
Description
Mouse monoclonal [3E8AD9] to GAPDH - Loading Control -
Host species
Mouse -
Tested applications
Suitable for: ICC/IF, WB, Flow Cyt, IPmore details -
Species reactivity
Reacts with: Human -
Immunogen
Full length protein. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: HepG2 cells IP: HepG2 cells and Human liver mitichondria Flow Cyt: HL-60 cells ICC/IF: Human HDFn cells
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General notes
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Product was previously marketed under the MitoSciences sub-brand.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.5
Preservative: 0.02% Sodium azide
Constituent: HEPES buffered saline -
Concentration information loading... -
Purification notes
ab110305 was produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation. Purity: >95% by SDS-PAGE. -
Clonality
Monoclonal -
Clone number
3E8AD9 -
Isotype
IgG2b -
Light chain type
kappa -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab110305 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF |
Use a concentration of 0.5 µg/ml.
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| WB |
Use a concentration of 0.125 µg/ml. Predicted molecular weight: 36 kDa.
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| Flow Cyt |
Use a concentration of 1 µg/ml.
ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody. |
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| IP |
Use at an assay dependent concentration.
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| Notes |
|---|
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ICC/IF
Use a concentration of 0.5 µg/ml. |
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WB
Use a concentration of 0.125 µg/ml. Predicted molecular weight: 36 kDa. |
|
Flow Cyt
Use a concentration of 1 µg/ml. ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody. |
|
IP
Use at an assay dependent concentration. |
Target
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Function
Has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively. Participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis. Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S-nitrosylation of nuclear target proteins such as SIRT1, HDAC2 and PRKDC (By similarity). Glyceraldehyde-3-phosphate dehydrogenase is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate. -
Pathway
Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 1/5. -
Sequence similarities
Belongs to the glyceraldehyde-3-phosphate dehydrogenase family. -
Post-translational
modificationsS-nitrosylation of Cys-152 leads to interaction with SIAH1, followed by translocation to the nucleus.
ISGylated. -
Cellular localization
Cytoplasm > cytosol. Nucleus. Cytoplasm > perinuclear region. Membrane. Translocates to the nucleus following S-nitrosylation and interaction with SIAH1, which contains a nuclear localization signal (By similarity). Postnuclear and Perinuclear regions. - Information by UniProt
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Database links
- Entrez Gene: 2597 Human
- Omim: 138400 Human
- SwissProt: P04406 Human
- Unigene: 544577 Human
- Unigene: 592355 Human
- Unigene: 598320 Human
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Alternative names
- 38 kDa BFA-dependent ADP-ribosylation substrate antibody
- aging associated gene 9 protein antibody
- Aging-associated gene 9 protein antibody
see all
Images
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Western blot - Anti-GAPDH antibody [3E8AD9] - Loading Control (ab110305)Anti-GAPDH antibody [3E8AD9] - Loading Control (ab110305) at 0.125 µg/ml + HepG2 cell lysate
Predicted band size: 36 kDa -
![Immunocytochemistry/ Immunofluorescence - Anti-GAPDH antibody [3E8AD9] - Loading Control (ab110305)](https://www.abcam.com/ps/products/110/ab110305/Images/ab110305-123568-anti-gapdh-antibody-3e8ad9-loading-control-immunofluorescence.gif)
Immunocytochemistry/ Immunofluorescence - Anti-GAPDH antibody [3E8AD9] - Loading Control (ab110305)Immunocytochemistry analysis using ab110305 at 0.5 µg/ml to stain GAPDH in HDFn cells (paraformaldehyde fixed and Triton X-100 permeabilized). Target locates primarily in the cytosol. -
![Immunoprecipitation - Anti-GAPDH antibody [3E8AD9] - Loading Control (ab110305)](https://www.abcam.com/ps/products/110/ab110305/Images/ab110305-123567-anti-gapdh-antibody-3e8ad9-loading-control-immunoprecipitation.gif)
Immunoprecipitation - Anti-GAPDH antibody [3E8AD9] - Loading Control (ab110305)Detection of ab110305 by Western Blot of Immunprecipitate.
Staining of GAPDH in HepG2 cells (lane 1) and Humman Liver Mitochondira (lane 2), immunoprecipitated using ab110305. -
![Flow Cytometry - Anti-GAPDH antibody [3E8AD9] - Loading Control (ab110305)](https://www.abcam.com/ps/products/110/ab110305/Images/ab110305-123566-anti-gapdh-antibody-3e8ad9-loading-control-flow-cytometry.gif)
Flow Cytometry - Anti-GAPDH antibody [3E8AD9] - Loading Control (ab110305)Flow cytometric analysis of GAPDH in HL60 cells (blue), using ab110305 at 1 µg/ml. Isotype control antibody (red).
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (9)
ab110305 has been referenced in 9 publications.
- Cui Y et al. miRNA-193a-3p Regulates the AKT2 Pathway to Inhibit the Growth and Promote the Apoptosis of Glioma Cells by Targeting ALKBH5. Front Oncol 11:600451 (2021). PubMed: 33968717
- Jin S et al. 5-Azacitidine Induces NOXA to Prime AML Cells for Venetoclax-Mediated Apoptosis. Clin Cancer Res 26:3371-3383 (2020). PubMed: 32054729
- Men L et al. microRNA-577 inhibits cell proliferation and invasion in non-small cell lung cancer by directly targeting homeobox A1. Mol Med Rep 19:1875-1882 (2019). PubMed: 30628697
- Zhao Z et al. MicroRNA-655 inhibits cell proliferation and invasion in epithelial ovarian cancer by directly targeting vascular endothelial growth factor. Mol Med Rep 18:1878-1884 (2018). PubMed: 29845206
- Li M et al. DNA damage-induced cell death relies on SLFN11-dependent cleavage of distinct type II tRNAs. Nat Struct Mol Biol 25:1047-1058 (2018). PubMed: 30374083
- Sun LL et al. Transplantation of betatrophin-expressing adipose-derived mesenchymal stem cells induces ß-cell proliferation in diabetic mice. Int J Mol Med 39:936-948 (2017). WB ; Human . PubMed: 28290605
- Parplys AC et al. NUCKS1 is a novel RAD51AP1 paralog important for homologous recombination and genome stability. Nucleic Acids Res 43:9817-34 (2015). WB ; Human . PubMed: 26323318
- Lin X et al. Silencing of Livin inhibits tumorigenesis and metastasis via VEGF and MMPs pathway in lung cancer. Int J Oncol 47:657-67 (2015). PubMed: 26094984
- Whelan RS et al. Bax regulates primary necrosis through mitochondrial dynamics. Proc Natl Acad Sci U S A 109:6566-71 (2012). PubMed: 22493254