Anti-GAPDH antibody - Loading Control (ab37168)
Key features and details
- Rabbit polyclonal to GAPDH - Loading Control
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-GAPDH antibody - Loading Control
See all GAPDH primary antibodies -
Description
Rabbit polyclonal to GAPDH - Loading Control -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide corresponding to Human GAPDH aa 60-110 (N terminal). Synthetic peptide, corresponding to 16 N terminal amino acids of Human GAPDH
Database link: P04406 -
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at +4°C. -
Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
GAPDH Antibody is affinity chromatography purified via peptide column. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab37168 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
Use a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 36 kDa (predicted molecular weight: 36 kDa).
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| IHC-P |
Use a concentration of 10 µg/ml.
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| ICC/IF |
Use a concentration of 0.1 - 1 µg/ml.
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| Notes |
|---|
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WB
Use a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 36 kDa (predicted molecular weight: 36 kDa). |
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IHC-P
Use a concentration of 10 µg/ml. |
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ICC/IF
Use a concentration of 0.1 - 1 µg/ml. |
Target
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Function
Has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively. Participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis. Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S-nitrosylation of nuclear target proteins such as SIRT1, HDAC2 and PRKDC (By similarity). Glyceraldehyde-3-phosphate dehydrogenase is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate. -
Pathway
Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 1/5. -
Sequence similarities
Belongs to the glyceraldehyde-3-phosphate dehydrogenase family. -
Post-translational
modificationsS-nitrosylation of Cys-152 leads to interaction with SIAH1, followed by translocation to the nucleus.
ISGylated. -
Cellular localization
Cytoplasm > cytosol. Nucleus. Cytoplasm > perinuclear region. Membrane. Translocates to the nucleus following S-nitrosylation and interaction with SIAH1, which contains a nuclear localization signal (By similarity). Postnuclear and Perinuclear regions. - Information by UniProt
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Database links
- Entrez Gene: 2597 Human
- Omim: 138400 Human
- SwissProt: P04406 Human
- Unigene: 544577 Human
- Unigene: 592355 Human
- Unigene: 598320 Human
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Alternative names
- 38 kDa BFA-dependent ADP-ribosylation substrate antibody
- aging associated gene 9 protein antibody
- Aging-associated gene 9 protein antibody
see all
Images
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Western blot - Anti-GAPDH antibody - Loading Control (ab37168)Lane 1 : Anti-GAPDH antibody - Loading Control (ab37168) at 0.125 µg/ml
Lane 2 : Anti-GAPDH antibody - Loading Control (ab37168) at 0.25 µg/ml
Lane 3 : Anti-GAPDH antibody - Loading Control (ab37168) at 0.5 µg/ml
All lanes : HeLa cell lysate with GAPDH peptide
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : anti-rabbit IgG HRP at 1/10000 dilution
Predicted band size: 36 kDa
Observed band size: 36 kDa -
Immunocytochemistry/ Immunofluorescence - Anti-GAPDH antibody - Loading Control (ab37168)ICC/IF image of ab37168 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab37168, 1mg/ml) overnight at +4øC. The secondary antibody (green) was anti-rabbit Alexa Fluor® 488 (ab150077) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43æM.
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Immunocytochemistry/ Immunofluorescence - Anti-GAPDH antibody - Loading Control (ab37168)ICC/IF image of ab37168 stained Hela cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab37168, 1µg/ml) overnight at +4°C. The secondary antibody (green) was anti-Rabbit DyLight® 488 pre-adsorbed (ab96899) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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Immunocytochemistry/ Immunofluorescence - Anti-GAPDH antibody - Loading Control (ab37168)Immunofluorescence of GAPDH in HeLa cells using ab37168 at 10 ug/ml.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GAPDH antibody - Loading Control (ab37168)ab37168 at 10µg/ml staining GAPDH in human liver tissue by IHC
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (445)
ab37168 has been referenced in 445 publications.
- Li C et al. MicroRNA‑194‑5p attenuates hypoxia/reoxygenation‑induced apoptosis in H9C2 cardiomyocytes by inhibiting the over‑activation of RAC1 protein. Mol Med Rep 27:N/A (2023). PubMed: 36562344
- Jiang S et al. Human bone marrow mesenchymal stem cells-derived exosomes attenuated prostate cancer progression via the miR-99b-5p/IGF1R axis. Bioengineered 13:2004-2016 (2022). PubMed: 35030978
- Zhang W et al. Tbx18 promoted the conversion of human-induced pluripotent stem cell-derived cardiomyocytes into sinoatrial node-like pacemaker cells. Cell Biol Int 46:403-414 (2022). PubMed: 34882885
- Shalomov B et al. A revised mechanism of action of hyperaldosteronism-linked mutations in cytosolic domains of GIRK4 (KCNJ5). J Physiol 600:1419-1437 (2022). PubMed: 34957562
- Bao W et al. Gout-associated uric acid crystals induce tophi ulcerations and impair wound healing in a novel gouty ulcer model. Wound Repair Regen 30:132-139 (2022). PubMed: 34665905