Recombinant Anti-Gastrin antibody [SP318] (ab223501)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Gastrin antibody [SP318] (ab223501)](https://www.abcam.com/ps/products/223/ab223501/Images/ab223501-2-AntiGastrin-antibody-SP318-IHC-human-stomach.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Gastrin antibody [SP318] (ab223501)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [SP318] to Gastrin
- Suitable for: IHC-P
- Reacts with: Human
Overview
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Product name
Anti-Gastrin antibody [SP318]
See all Gastrin primary antibodies -
Description
Rabbit monoclonal [SP318] to Gastrin -
Host species
Rabbit -
Tested applications
Suitable for: IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human Gastrin (internal sequence). The exact sequence is proprietary.
Database link: P01350 -
Positive control
- IHC-P: Human stomach tissue.
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General notes
This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.60
Preservative: 0.1% Sodium azide
Constituents: PBS, 1% BSA -
Concentration information loading... -
Purity
Protein A/G purified -
Clonality
Monoclonal -
Clone number
SP318 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab223501 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P |
1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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| Notes |
|---|
|
IHC-P
1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
Target
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Function
Gastrin stimulates the stomach mucosa to produce and secrete hydrochloric acid and the pancreas to secrete its digestive enzymes. It also stimulates smooth muscle contraction and increases blood circulation and water secretion in the stomach and intestine. -
Sequence similarities
Belongs to the gastrin/cholecystokinin family. -
Post-translational
modificationsTwo different processing pathways probably exist in antral G-cells. In the dominant pathway progastrin is cleaved at three sites resulting in two major bioactive gastrins, gastrin-34 and gastrin-17. In the putative alternative pathway, progastrin may be processed only at the most C-terminal dibasic site resulting in the synthesis of gastrin-71.
Sulfation enhances proteolytic processing, and blocks peptide degradation. Levels of sulfation differ between proteolytically-cleaved gastrins. Thus, gastrin-6 is almost 73% sulfated, whereas the larger gastrins are less than 50% sulfated. Sulfation levels are also tissue-specific. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 2520 Human
- Omim: 137250 Human
- SwissProt: P01350 Human
- Unigene: 2681 Human
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Alternative names
- Component II antibody
- G14 antibody
- G17 antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Gastrin antibody [SP318] (ab223501)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human stomach tissue sections labeling Gastrin with ab223501 at 1/100 dilution (1.08 μg/ml). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 10 mins. Goat Anti-Rabbit & Mouse IgG (HRP) was used as the secondary antibody. Hematoxylin was used as a counterstain. Sporadic positive staining on the human stomach, performed on a Leica Biosystems BOND™ RX instrument.
The section was incubated with ab223501 for 10 mins at room temperature. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Gastrin antibody [SP318] (ab223501)](https://www.abcam.com/ps/products/223/ab223501/Images/ab223501-283009-anti-gastrin-antibody-sp318-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Gastrin antibody [SP318] (ab223501)Paraffin-embedded human stomach tissue stained for Gastrin using ab223501 at 1/100 dilution in immunohistochemical analysis.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab223501 has not yet been referenced specifically in any publications.