Recombinant Anti-GATA2 antibody [EPR2822(2)] (ab109241)
![Immunoprecipitation - Anti-GATA2 antibody [EPR2822(2)] (ab109241)](https://www.abcam.com/ps/products/109/ab109241/Images/ab109241-6-anti-gata2-antibody-epr28222-immunoprecipitation-k-5621.jpg "Immunoprecipitation - Anti-GATA2 antibody [EPR2822(2)] (ab109241)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR2822(2)] to GATA2
- Suitable for: ChIP, WB, IP
- Knockout validated
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-GATA2 antibody [EPR2822(2)]
See all GATA2 primary antibodies -
Description
Rabbit monoclonal [EPR2822(2)] to GATA2 -
Host species
Rabbit -
Tested applications
Suitable for: ChIP, WB, IPmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: bEnd.3, PC-12, HEK-293, and K562 whole cell lysates. Mouse placenta lysate; IP: K-562 whole cell lysate.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR2822(2) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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KO cell lines
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KO cell lysates
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab109241 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ChIP |
Use at an assay dependent concentration.
|
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| WB |
1/1000. Predicted molecular weight: 51 kDa.
For unpurified use at 1/1000 - 1/10000. |
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| IP |
1/30.
For unpurified use at 1/10 - 1/100. |
| Notes |
|---|
|
ChIP
Use at an assay dependent concentration. |
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WB
1/1000. Predicted molecular weight: 51 kDa. For unpurified use at 1/1000 - 1/10000. |
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IP
1/30. For unpurified use at 1/10 - 1/100. |
Target
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Function
Transcriptional activator which regulates endothelin-1 gene expression in endothelial cells. Binds to the consensus sequence 5'-AGATAG-3'. -
Tissue specificity
Endothelial cells. -
Sequence similarities
Contains 2 GATA-type zinc fingers. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 2624 Human
- Entrez Gene: 14461 Mouse
- Entrez Gene: 25159 Rat
- Omim: 137295 Human
- SwissProt: P23769 Human
- SwissProt: O09100 Mouse
- SwissProt: Q924Y4 Rat
- Unigene: 367725 Human
see all -
Alternative names
- DCML antibody
- Endothelial transcription factor GATA 2 antibody
- Endothelial transcription factor GATA-2 antibody
see all
Images
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Immunoprecipitation - Anti-GATA2 antibody [EPR2822(2)] (ab109241)GATA2 was immunoprecipitated from 0.35 mg K-562 (Human chronic myelogenous leukemia lymphoblast) cell lysate 10 µg with ab109241 at 1/30 dilution (2µg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab109241 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.
Lane 1: K-562 (Human chronic myelogenous leukemia lymphoblast) cell lysate 10 µg
Lane 2: ab109241 IP in K-562 cell lysate
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab109241 in K562 cell lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 8 seconds
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![Western blot - Anti-GATA2 antibody [EPR2822(2)] (ab109241)](https://www.abcam.com/ps/products/109/ab109241/Images/ab109241-353566-antigata2antibodyepr28222westernblotknockouthek293gata2humanlysates.jpg)
Western blot - Anti-GATA2 antibody [EPR2822(2)] (ab109241)All lanes : Anti-GATA2 antibody [EPR2822(2)] (ab109241) at 1 µg/ml
Lane 1 : Wild-type HEK-293 whole cell lysate
Lane 2 : GATA2 knockout HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 51 kDaLanes 1 - 2: Merged signal (red and green). Green - ab109241 observed at 50 kDa. Red - loading control, ab8245, observed at 37 kDa.
ab109241 was shown to recognize GATA2 in wild-type HEK-293 cells as signal was lost at the expected MW in GATA2 knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and GATA2 knockout samples were subjected to SDS-PAGE. The membrane was blocked with 3% Milk. Ab109241 and ab8245 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1 μg/ml and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
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![Immunoprecipitation - Anti-GATA2 antibody [EPR2822(2)] (ab109241)](https://www.abcam.com/ps/products/109/ab109241/Images/ab109241-4-anti-gata2-antibody-epr2822-2-immunoprecipitation-k562-human.jpg)
Immunoprecipitation - Anti-GATA2 antibody [EPR2822(2)] (ab109241)Purified ab109241 at 1/30 dilution (2 µg) immunoprecipitating GATA2 in K-562 whole cell lysate.
Lane 1 (input): K-562 (Human chronic myelogenous leukemia lymphoblast) whole cell lysate 10 µg
Lane 2 (+): ab109241 + K-562 whole cell lysate.
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab109241 in K-562 whole cell lysate.
VeriBlot for IP Detection Reagent (HRP) (ab131366) (1/1000 dilution) was used for Western blotting.
Blocking Buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM/TBST.
Observed band size: 51 kDa -
![Western blot - Anti-GATA2 antibody [EPR2822(2)] (ab109241)](https://www.abcam.com/ps/products/109/ab109241/Images/ab109241-396912-anti-gata2-antibody-epr2822-2-westernblot-mouse-rat.jpg)
Western blot - Anti-GATA2 antibody [EPR2822(2)] (ab109241)All lanes : Anti-GATA2 antibody [EPR2822(2)] (ab109241) at 1/1000 dilution (purified)
Lane 1 : bEnd.3 (Mouse brain endothelioma) whole cell lysate
Lane 2 : Mouse placenta lysate
Lane 3 : PC-12 (Rat adrenal gland pheochromocytoma) whole cell lysate
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 51 kDa
Observed band size: 51 kDa -
![Western blot - Anti-GATA2 antibody [EPR2822(2)] (ab109241)](https://www.abcam.com/ps/products/109/ab109241/Images/ab109241-302820-anti-gata2-antibody-epr28222-western-blot.jpg)
Western blot - Anti-GATA2 antibody [EPR2822(2)] (ab109241)All lanes : Anti-GATA2 antibody [EPR2822(2)] (ab109241) at 1/1000 dilution
Lane 1 : K-562 (Human chronic myelogenous leukemia lymphoblast) whole cell lysate prepared using 1% SDS hot lysis method
Lane 2 : K-562 (Human chronic myelogenous leukemia lymphoblast) whole cell lysate prepared using RIPA lysis method
Lane 3 : U-937 (Human histiocytic lymphoma monocyte) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/2000 dilution
Predicted band size: 51 kDa
Exposure time: 3 minutesBlocking and diluting buffer: 5% NFDM/TBST.
The different result in K-562 is due to the lysates preparation method.
For Lysate preparation protocol, please refer to the protocol book in the protocol section and/or here (downloadable copy).
The expression profile observed in U-937 is consistent with the literature (PMID: 19212333).
Negative control: U-937 (PMID: 19212333) -
![Anti-GATA2 antibody [EPR2822(2)] (ab109241)](https://www.abcam.com/ps/products/109/ab109241/Images/ab109241-7-benefits-of-recombinant-antibodies.png)
Anti-GATA2 antibody [EPR2822(2)] (ab109241)
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (8)
ab109241 has been referenced in 8 publications.
- Milano-Foster J et al. Regulation of human trophoblast syncytialization by histone demethylase LSD1. J Biol Chem 294:17301-17313 (2019). PubMed: 31591264
- Bergiers I et al. Single-cell transcriptomics reveals a new dynamical function of transcription factors during embryonic hematopoiesis. Elife 7:N/A (2018). PubMed: 29555020
- Zhang Z et al. O-GlcNAc homeostasis contributes to cell fate decisions during hematopoiesis. J Biol Chem N/A:N/A (2018). PubMed: 30523150
- Wang K et al. Correction of Anemia in Chronic Kidney Disease With Angelica sinensis Polysaccharide via Restoring EPO Production and Improving Iron Availability. Front Pharmacol 9:803 (2018). PubMed: 30108502
- Fan J et al. A novel role of CKIP-1 in promoting megakaryocytic differentiation. Oncotarget 8:30138-30150 (2017). PubMed: 28404913
- Qiu C et al. The critical role of SENP1-mediated GATA2 deSUMOylation in promoting endothelial activation in graft arteriosclerosis. Nat Commun 8:15426 (2017). WB ; Mouse, Human . PubMed: 28569748
- Lu W et al. Calf Spleen Extractive Injection protects mice against cyclophosphamide-induced hematopoietic injury through G-CSF-mediated JAK2/STAT3 signaling. Sci Rep 7:8402 (2017). PubMed: 28827748
- Nakajima T et al. Regulation of GATA-binding protein 2 levels via ubiquitin-dependent degradation by Fbw7: involvement of cyclin B-cyclin-dependent kinase 1-mediated phosphorylation of THR176 in GATA-binding protein 2. J Biol Chem 290:10368-81 (2015). PubMed: 25670854