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RecombinantRabMAb

Recombinant Anti-GATA3 (phospho S308) antibody [EPR18118] (ab186371)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GATA3 (phospho S308) antibody [EPR18118] (ab186371)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GATA3 (phospho S308) antibody [EPR18118] (ab186371)
  • Dot Blot - Anti-GATA3 (phospho S308) antibody [EPR18118] (ab186371)
  • Western blot - Anti-GATA3 (phospho S308) antibody [EPR18118] (ab186371)
  • Western blot - Anti-GATA3 (phospho S308) antibody [EPR18118] (ab186371)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GATA3 (phospho S308) antibody [EPR18118] (ab186371)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GATA3 (phospho S308) antibody [EPR18118] (ab186371)
  • Immunoprecipitation - Anti-GATA3 (phospho S308) antibody [EPR18118] (ab186371)
  • Dot Blot - Anti-GATA3 (phospho S308) antibody [EPR18118] (ab186371)
  • Anti-GATA3 (phospho S308) antibody [EPR18118] (ab186371)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR18118] to GATA3 (phospho S308)
  • Suitable for: WB, IHC-P, Dot blot, IP
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Carrier Free

Overview

  • Product name

    Anti-GATA3 (phospho S308) antibody [EPR18118]
    See all GATA3 primary antibodies

  • Description

    Rabbit monoclonal [EPR18118] to GATA3 (phospho S308)

  • Host species

    Rabbit

  • Specificity

    The immunogen sequence for ab186371 is identical to the sequence around S340 of GATA2 (UniProt P23769); however the phosphorylation of GATA2 on S340 has not been reported.

  • Tested applications

    Suitable for: WB, IHC-P, Dot blot, IPmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Jurkat whole cell lysate and EL4 cell lysate treated with 1mM 8-Bromo-cAMP for 6 hours. IHC-P: Human placenta tissue and Human transitional cell carcinoma tissues; Mouse and rat stomach tissue. IP: Jurkat whole cell lysate treated with 1mM 8-Bromo-cAMP for 6 hours.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab186371 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/10000. Detects a band of approximately 48 kDa (predicted molecular weight: 48 kDa).
IHC-P (1)
1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Dot blot
1/1000.
IP
1/70.
Notes
WB
1/10000. Detects a band of approximately 48 kDa (predicted molecular weight: 48 kDa).
IHC-P
1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Dot blot
1/1000.
IP
1/70.

Target

  • Function

    Transcriptional activator which binds to the enhancer of the T-cell receptor alpha and delta genes. Binds to the consensus sequence 5'-AGATAG-3'.

  • Tissue specificity

    T-cells and endothelial cells.

  • Involvement in disease

    Defects in GATA3 are the cause of hypoparathyroidism with sensorineural deafness and renal dysplasia (HDR) [MIM:146255]; also known as Barakat syndrome.

  • Sequence similarities

    Contains 2 GATA-type zinc fingers.

  • Cellular localization

    Nucleus.
  • Information by UniProt

  • Database links

    • Form

      There are 2 isoforms produced by alternative splicing.

    • Alternative names

      • GATA 3 antibody
      • GATA binding factor 3 antibody
      • GATA binding protein 3 antibody
      • GATA-binding factor 3 antibody
      • Gata3 antibody
      • GATA3_HUMAN antibody
      • HDR antibody
      • HDRS antibody
      • MGC2346 antibody
      • MGC5199 antibody
      • MGC5445 antibody
      • Trans acting T cell specific transcription factor GATA 3 antibody
      • Trans-acting T-cell-specific transcription factor GATA-3 antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GATA3 (phospho S308) antibody [EPR18118] (ab186371)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GATA3 (phospho S308) antibody [EPR18118] (ab186371)

      Immunohistochemical analysis of paraffin-embedded Rat stomach tissue labeling GATA3 (phospho S308) with ab186371 at 1/50 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). The section was counterstained with Hematoxylin. Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101)
      Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

      Nuclear staining on rat stomach without Lambda Protein Phosphatase treatment (image A). No signal was detected when tissues were treated with Lambda Protein Phosphatase treatment (image B).
      The section was incubated with ab186371 for 30 mins at room temperature.
      The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GATA3 (phospho S308) antibody [EPR18118] (ab186371)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GATA3 (phospho S308) antibody [EPR18118] (ab186371)

      Immunohistochemical analysis of paraffin-embedded Mouse stomach tissue labeling GATA3 (phospho S308) with ab186371 at 1/50 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). The section was counterstained with Hematoxylin. Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101)
      Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

      Nuclear staining on mouse stomach without Lambda Protein Phosphatase treatment (image A). No signal was detected when tissues were treated with Lambda Protein Phosphatase treatment (image B).
      The section was incubated with ab186371 for 30 mins at room temperature.
      The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

    • Dot Blot - Anti-GATA3 (phospho S308) antibody [EPR18118] (ab186371)
      Dot Blot - Anti-GATA3 (phospho S308) antibody [EPR18118] (ab186371)

      Dot blot analysis of GATA3 (phospho S308) using ab186371 at 1:1000 (1.121 μg/ml) followed by a Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated antibody (ab97051) at 1:100000 dilution.

      Lane 1: Human GATA3 (phospho Ser316) peptide
      Lane 2: Human GATA3 (phospho Thr315) peptide
      Lane 3: Human GATA3 (phospho Ser308) peptide

      Blocking and diluting buffer and concentration: 5% NFDM/TBST.

      Exposure time: 180 seconds

    • Western blot - Anti-GATA3 (phospho S308) antibody [EPR18118] (ab186371)
      Western blot - Anti-GATA3 (phospho S308) antibody [EPR18118] (ab186371)
      All lanes : Anti-GATA3 (phospho S308) antibody [EPR18118] (ab186371) at 1/1000 dilution

      Lane 1 : Untreated EL4 (mouse lymphoma T lymphocyte) whole cell lysate
      Lane 2 : EL4 treated with 0.5 mM 8-Bromo-cAMP for 6 hours whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

      Predicted band size: 48 kDa
      Observed band size: 48 kDa


      Exposure time: 70 seconds


      Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    • Western blot - Anti-GATA3 (phospho S308) antibody [EPR18118] (ab186371)
      Western blot - Anti-GATA3 (phospho S308) antibody [EPR18118] (ab186371)
      All lanes : Anti-GATA3 (phospho S308) antibody [EPR18118] (ab186371) at 1/10000 dilution

      Lane 1 : Untreated Jurkat (Human T cell leukemia cells from peripheral blood) whole cell lysates
      Lane 2 : Jurkat whole cell lysates treated with 1mM 8-Bromo-cAMP for 6 hours

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

      Predicted band size: 48 kDa
      Observed band size: 48 kDa


      Exposure time: 15 seconds


      Blocking/dilution buffer: 5% NFDM/TBST.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GATA3 (phospho S308) antibody [EPR18118] (ab186371)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GATA3 (phospho S308) antibody [EPR18118] (ab186371)

      Immunohistochemical analysis of paraffin-embedded Human transitional cell carcinoma tissue labeling GATA3 (phospho S308) with ab186371 at 1/100 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Nucleus staining on tumor cells of Human transitional cell carcinoma of bladder is observed. Counter stained with Hematoxylin.

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

      Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GATA3 (phospho S308) antibody [EPR18118] (ab186371)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GATA3 (phospho S308) antibody [EPR18118] (ab186371)

      Immunohistochemical analysis of paraffin-embedded Human placenta tissue labeling GATA3 (phospho S308) with ab186371 at 1/100 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Nucleus staining on trophoblast cells of Human placenta is observed. Counter stained with Hematoxylin.

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

      Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    • Immunoprecipitation - Anti-GATA3 (phospho S308) antibody [EPR18118] (ab186371)
      Immunoprecipitation - Anti-GATA3 (phospho S308) antibody [EPR18118] (ab186371)

      GATA3 (phospho S308) was immunoprecipitated from 1mg of Jurkat (Human T cell leukemia cells from peripheral blood) cells treated with 1mM 8-Bromo-cAMP for 6 hours with ab186371 at 1/70 dilution. Western blot was performed from the immunoprecipitate using ab186371 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1/1500 dilution.

      Lane 1: Jurkat whole cell lysate treated with 1mM 8-Bromo-cAMP for 6 hours, 10 µg (Input).

      Lane 2: ab186371 IP in Jurkat whole cell lysate treated with 1mM 8-Bromo-cAMP for 6 hours.

      Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab186371 in Jurkat whole cell lysate treated with 1mM 8-Bromo-cAMP for 6 hours.
      Blocking and dilution buffer and concentration: 5% NFDM/TBST.

      Exposure time: 10 seconds.

    • Dot Blot - Anti-GATA3 (phospho S308) antibody [EPR18118] (ab186371)
      Dot Blot - Anti-GATA3 (phospho S308) antibody [EPR18118] (ab186371)

      Dot blot analysis of GATA3 (phospho S308) peptide (Lane 1) and non-phospho peptide (Lane 2) labeled using ab186371 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated secondary antibody at 1/1000 dilution.

      Blocking/Dilution buffer: 5% NFDM/TBST..

      Exposure time: 3 minutes.

    • Anti-GATA3 (phospho S308) antibody [EPR18118] (ab186371)
      Anti-GATA3 (phospho S308) antibody [EPR18118] (ab186371)

    References (1)

    Publishing research using ab186371? Please let us know so that we can cite the reference in this datasheet.

    ab186371 has been referenced in 1 publication.

    • Sereesongsaeng N  et al. Cathepsin V suppresses GATA3 protein expression in luminal A breast cancer. Breast Cancer Res 22:139 (2020). PubMed: 33298139

    Customer reviews and Q&As

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    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-GATA3 (phospho S308) antibody [EPR18118]

     Excellent
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (breast tumor)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: citrate ph6
    Permeabilization
    No
    Specification
    breast tumor
    Blocking step
    BSA as blocking agent for 30 minute(s) · Concentration: 2.5% · Temperature: 24°C
    Fixative
    Paraformaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    DR. Cecilia Proietti

    Verified customer

    Submitted Oct 06 2016

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