Recombinant Anti-GATA4 antibody [EPR26718-103] (ab307823)

Product name

Anti-GATA4 antibody [EPR26718-103]
See all GATA4 primary antibodies
Description

Rabbit monoclonal [EPR26718-103] to GATA4
Host species

Rabbit
Specificity

Unsuitable:

WB: Human

IHC-FR: Mouse
Tested applications

Suitable for: Flow Cyt (Intra), IP, IHC-P, WB, ICC/IFmore details
Unsuitable for: IHC-Fr
Species reactivity

Reacts with: Mouse, Rat, Human
Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: Mouse testis, Mouse P7 ovary, Mouse skeletal muscle, Rat P7 testis, Rat P7 ovary, and Rat skeletal muscle lysates. IHC-P: Human testis, Mouse testis, Rat testis, Human skeletal muscle, Mouse skeletal muscle, and Rat skeletal muscle tissues. ICC: Huh7 cells Flow Cyt: HeLa cell IP: Mouse P7 testis cell.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR26718-103
Isotype

IgG
Research areas

Alternative Versions
- Anti-GATA4 antibody [EPR26718-103] - BSA and Azide free (ab307824)
Isotype control
- Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
Related Products

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab307823 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application	Abreviews	Notes
Flow Cyt (Intra)		1/500.
IP		1/30.
IHC-P		1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB		1/1000. Detects a band of approximately 56 kDa.
ICC/IF		1/50.

Notes
Flow Cyt (Intra) 1/500.
IP 1/30.
IHC-P 1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB 1/1000. Detects a band of approximately 56 kDa.
ICC/IF 1/50.

Application notes

Is unsuitable for IHC-Fr.

Function

Transcriptional activator that binds to the consensus sequence 5'-AGATAG-3' and plays a key role in cardiac development (PubMed:24000169). Involved in bone morphogenetic protein (BMP)-mediated induction of cardiac-specific gene expression (By similarity). Binds to BMP response element (BMPRE) DNA sequences within cardiac activating regions (By similarity). Acts as a transcriptional activator of ANF in cooperation with NKX2-5 (By similarity). Promotes cardiac myocyte enlargement (PubMed:20081228). Required during testicular development (PubMed:21220346). May play a role in sphingolipid signaling by regulating the expression of sphingosine-1-phosphate degrading enzyme, spingosine-1-phosphate lyase (PubMed:15734735).
Involvement in disease

Atrial septal defect 2
Ventricular septal defect 1
Tetralogy of Fallot
Atrioventricular septal defect 4
Testicular anomalies with or without congenital heart disease
GATA4 mutations can predispose to dilated cardiomyopathy (CMD), a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Patients are at risk of premature death.
Sequence similarities

Contains 2 GATA-type zinc fingers.
Post-translational
modifications

Methylation at Lys-300 attenuates transcriptional activity.
Cellular localization

Nucleus.
Target information above from: UniProt accession P43694 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
Database links
- Entrez Gene: 2626 Human
- Entrez Gene: 14463 Mouse
- Entrez Gene: 54254 Rat
- Omim: 600576 Human
- SwissProt: P43694 Human
- SwissProt: Q08369 Mouse
- SwissProt: P46152 Rat
- Unigene: 243987 Human
- Unigene: 247669 Mouse
- Unigene: 26251 Rat
see all
Alternative names
- ASD2 antibody
- GATA 4 antibody
- GATA binding protein 4 antibody
- GATA-binding factor 4 antibody
- gata4 antibody
- GATA4_HUMAN antibody
- MGC126629 antibody
- Transcription factor GATA 4 antibody
- Transcription factor GATA-4 antibody
- Transcription factor GATA4 antibody
- VSD1 antibody
see all

Flow Cytometry (Intracellular) - Anti-GATA4 antibody [EPR26718-103] (ab307823)

Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized HeLa (human cervical adenocarcinoma epithelial cell, Left) / Huh7(human hepatocellular carcinoma epithelial cell, Right) cells labelling GATA4 with ab307823 at 1/500 dilution (0.1ug) (Red) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/5000 dilution was used as the secondary antibody.
Immunoprecipitation - Anti-GATA4 antibody [EPR26718-103] (ab307823)

GATA4 was immunoprecipitated from 0.35 mg Mouse P7 testis tissue lysate with ab307823 at 1/30 dilution (2μg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab307823 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution.

Lane 1: Mouse P7 testis tissue lysate 10 μg

Lane 2: ab307823 IP in Mouse P7 testis tissue lysate

Lane 3:Rabbit monoclonal IgG (ab172730) instead of ab307823 in mouse P7 testis tissue lysate

Blocking and dilution buffer and concentration: 5% NFDM/TBST.

Exposure time: 81 seconds
Immunocytochemistry/ Immunofluorescence - Anti-GATA4 antibody [EPR26718-103] (ab307823)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized Huh7 (human hepatocellular carcinoma epithelial cell) cells labelling GATA4 with ab307823 at 1/50 (9.82 μg/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2 μg/ml dilution (Green). Confocal image showing nuclear staining in Huh7 cell line. Negative control: HeLa (PMID: 24696446).Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8). is observed. ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5μg/ml dilution (Red). The Nuclear counterstain was DAPI (Blue).

Secondary antibody only control: Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 μg/ml dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GATA4 antibody [EPR26718-103] (ab307823)

Immunohistochemical analysis of paraffin-embedded Rat skeletal muscle tissue labeling GATA4 with ab307823 at 1/4000 (0.123 μg/ml) followed by a LeicaDS9800 (Bond™ Polymer Refine Detection) at Ready to use dilution. Negative control: No staining on rat skeletal muscle. The section was incubated with ab307823 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is LeicaDS9800 (Bond™ Polymer Refine Detection) at Ready to use dilution.

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GATA4 antibody [EPR26718-103] (ab307823)

Immunohistochemical analysis of paraffin-embedded Mouse skeletal muscle tissue labeling GATA4 with ab307823 at 1/4000 (0.123 μg/ml) followed by a LeicaDS9800 (Bond™ Polymer Refine Detection) at Ready to use dilution. Negative control: No staining on mouse skeletal muscle. The section was incubated with ab307823 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is LeicaDS9800 (Bond™ Polymer Refine Detection) at Ready to use dilution.

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GATA4 antibody [EPR26718-103] (ab307823)

Immunohistochemical analysis of paraffin-embedded Human skeletal muscle tissue labeling GATA4 with ab307823 at 1/500 (0.982 μg/ml) followed by a LeicaDS9800 (Bond™ Polymer Refine Detection) at Ready to use dilution. Negative control: No staining on human skeletal muscle. The section was incubated with ab307823 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is LeicaDS9800 (Bond™ Polymer Refine Detection) at Ready to use dilution.

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GATA4 antibody [EPR26718-103] (ab307823)

Immunohistochemical analysis of paraffin-embedded Rat testis tissue labeling GATA4 with ab307823 at 1/4000 (0.123 μg/ml) followed by a LeicaDS9800 (Bond™ Polymer Refine Detection) at Ready to use dilution. Positive staining on rat testis. The section was incubated with ab307823 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is LeicaDS9800 (Bond™ Polymer Refine Detection) at Ready to use dilution.

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GATA4 antibody [EPR26718-103] (ab307823)

Immunohistochemical analysis of paraffin-embedded (A) Mouse stomach (tissue labeling GATA4 with ab307823 at 1/4000 (0.123 μg/ml) followed by a LeicaDS9800 (Bond™ Polymer Refine Detection) at Ready to use dilution. Positive staining on (A) mouse stomach (PMID: 34111600). No staining on (B) mouse colon (PMID: 18405344). The section was incubated with ab307823 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is LeicaDS9800 (Bond™ Polymer Refine Detection) at Ready to use dilution.

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GATA4 antibody [EPR26718-103] (ab307823)

Immunohistochemical analysis of paraffin-embedded Mouse testis tissue labeling GATA4 with ab307823 at 1/4000 (0.123 μg/ml) followed by a LeicaDS9800 (Bond™ Polymer Refine Detection) at Ready to use dilution. Positive staining on mouse testis (PMID: 30793514). The section was incubated with ab307823 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is LeicaDS9800 (Bond™ Polymer Refine Detection) at Ready to use dilution.

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GATA4 antibody [EPR26718-103] (ab307823)

Immunohistochemical analysis of paraffin-embedded Human testis tissue labeling GATA4 with ab307823 at 1/500 (0.982 μg /ml) followed by a LeicaDS9800 (Bond™ Polymer Refine Detection) at Ready to use dilution. Positive staining on human testis. The section was incubated with ab307823 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is LeicaDS9800 (Bond™ Polymer Refine Detection) at Ready to use dilution.

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Western blot - Anti-GATA4 antibody [EPR26718-103] (ab307823)

All lanes : Anti-GATA4 antibody [EPR26718-103] (ab307823) at 1/1000 dilution

Lane 1 : Mouse testis tissue lysate 20 µg with NFDM/TBST
Lane 2 : Mouse P7 ovary tissue lysate 20 µg with NFDM/TBST
Lane 3 : Mouse skeletal muscle tissue lysate 20 µg with NFDM/TBST
Lane 4 : Rat P7 testis tissue lysate 20 µg with NFDM/TBST
Lane 5 : Rat P7 ovary tissue lysate 20 µg with NFDM/TBST
Lane 6 : Rat skeletal muscle tissue lysate 20 µg with NFDM/TBST

Blocking peptides at 5 % per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051))

Negative control: skeletal muscle (PMID:8455608).
The identity of the lower MW band at approximately 35 kDa is unknown.
In Western blot, anti-GAPDH antibody (ab181602) loading control staining at 1/200,000 dilution.
Anti-GATA4 antibody [EPR26718-103] (ab307823)

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

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Key features and details

Related conjugates and formulations

Overview

Product name

Description

Host species

Specificity

Tested applications

Species reactivity

Immunogen

Positive control

General notes

Properties

Form

Storage instructions

Storage buffer

Purity

Clonality

Clone number

Isotype

Research areas

Associated products

Alternative Versions

Isotype control

Related Products

Applications

The Abpromise guarantee

Target

Function

Involvement in disease

Sequence similarities

Post-translationalmodifications

Cellular localization

Database links

Alternative names

Images

Protocols

Datasheets and documents

SDS download

Datasheet download

Certificate of Compliance

References (0)

Customer reviews and Q&As

Post-translational
modifications