Anti-GATAD2A antibody (ab87663)
Key features and details
- Rabbit polyclonal to GATAD2A
- Suitable for: ICC/IF, WB, IP, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
- Long-term and scalable supply – powered by recombinant technology for fast production
- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-GATAD2A antibody
See all GATAD2A primary antibodies -
Description
Rabbit polyclonal to GATAD2A -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, WB, IP, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Dog, Turkey, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Platypus
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Immunogen
Synthetic peptide within Human GATAD2A aa 550 to the C-terminus. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements. (AAO31797.1)
Database link: Q86YP4 -
Positive control
- WB: HeLa and HEK-293T whole cell lysate. IP: HeLa whole cell lysate. IHC-P: Human breast carcinoma tissue. Mouse renal cell carcinoma tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab87663 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | (1) |
Use at an assay dependent concentration.
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| WB | (1) |
1/2000 - 1/10000. Predicted molecular weight: 68 kDa.
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| IP |
Use at 2-10 µg/mg of lysate.
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| IHC-P | (1) |
1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| Notes |
|---|
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ICC/IF
Use at an assay dependent concentration. |
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WB
1/2000 - 1/10000. Predicted molecular weight: 68 kDa. |
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IP
Use at 2-10 µg/mg of lysate. |
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IHC-P
1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
Transcriptional repressor. -
Tissue specificity
Ubiquitous, both in fetal and adult tissues. -
Sequence similarities
Contains 1 GATA-type zinc finger. -
Cellular localization
Nucleus speckle. - Information by UniProt
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Database links
- Entrez Gene: 508384 Cow
- Entrez Gene: 54815 Human
- Entrez Gene: 234366 Mouse
- Entrez Gene: 290669 Rat
- SwissProt: Q86YP4 Human
- SwissProt: Q8CHY6 Mouse
- Unigene: 118964 Human
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Alternative names
- FLJ20085 antibody
- FLJ21017 antibody
- GATA zinc finger domain containing 2A antibody
see all
Images
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Western blot - Anti-GATAD2A antibody (ab87663)All lanes : Anti-GATAD2A antibody (ab87663) at 0.1 µg/ml
Lane 1 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lysates/proteins at 50 µg per lane.
Predicted band size: 68 kDa
Exposure time: 3 minutesLysates prepared using NETN lysis buffer.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GATAD2A antibody (ab87663)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling GATAD2A with ab87663 at 1/1000 (1µg/ml). Detection: DAB.
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Immunoprecipitation - Anti-GATAD2A antibody (ab87663)Detection of human GATAD2A by western blot of immunoprecipitates.
Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from Hela cells prepared using NETN lysis buffer.
Antibodies: ab87663 used for IP at 6 µg per reaction. For blotting immunoprecipitated GATAD2A, ab87663 was used at 1 µg/ml. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GATAD2A antibody (ab87663)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse renal cell carcinoma tissue labelling GATAD2A with ab87663 at 1/1000 (1µg/ml). Detection: DAB.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (6)
ab87663 has been referenced in 6 publications.
- Pantier R et al. SALL4 controls cell fate in response to DNA base composition. Mol Cell 81:845-858.e8 (2021). PubMed: 33406384
- Yu X et al. Disruption of the MBD2-NuRD complex but not MBD3-NuRD induces high level HbF expression in human adult erythroid cells. Haematologica 104:2361-2371 (2019). PubMed: 31004025
- Salamun SG et al. The Epstein-Barr Virus BMRF1 Protein Activates Transcription and Inhibits the DNA Damage Response by Binding NuRD. J Virol 93:N/A (2019). PubMed: 31462557
- Kim MY et al. Mbd2-CP2c loop drives adult-type globin gene expression and definitive erythropoiesis. Nucleic Acids Res 46:4933-4949 (2018). PubMed: 29547954
- Witwicki RM et al. TRPS1 Is a Lineage-Specific Transcriptional Dependency in Breast Cancer. Cell Rep 25:1255-1267.e5 (2018). PubMed: 30380416
- Stephen TL et al. SATB1 Expression Governs Epigenetic Repression of PD-1 in Tumor-Reactive T Cells. Immunity 46:51-64 (2017). PubMed: 28099864