Anti-GBA antibody [2E2] (ab55080)

Thank you for contacting us and sorry for the delay in getting back to you.

We are not able to provide SDS documents in Italian but the English version can now be accessed from the datasheet of ab55080, or you will find it attached to this email.

I hope this has been of help If you have any further requests, please do not hesitate to contact us again.

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This antibody does not have any hazardous material in it so MSDS will not make any sense. For antibodies where hazardous material is used to preserve the solution the MSDS can be downloaded from online datasheet.

I hope this information will be helpful. Should you have any question please do not hesitate to contact me.

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We unfortunately do not provide MSDS in Italian language. We have facility to provide MSDS in Chinese, Japanese, English, Spanish, French and German languages but not Italian.

I hope this information is nevertheless helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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Thank you for your response.

A lysate ofPeptostreptococcus magnus,purified Protein L protein or recombinant Protein Lwould all be ideal positive controls for use withab63506.

Fora positive control to use with our anti GBA antibody, ab55080. We have data on our website which shows the antibody to work withMCF-7 cell lysate and withHN10 cells. I would recommend one of those lysates for a positive control.

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When choosing a loading control it is best practice to choose housekeeping genes which will be present in your sample type. For example, in whole cell preparations beta Actin or GAPDH will be appropriate while in nuclear or mitochondrial fractions use Lamin B1 or COXIV respectively.

These controls are excellent when you sample is eukaryotic. However, as Protein L will be from bacteria these housekeeping proteins may not be present. I have found however that GAPDH may be found in E.coli.

I would recommend therefore that if your sample types are whole cell/membrane or cytoplasmic and are either bacterial (in the case of the Protein L antibody ab63506) or eukaryotic (as for use with anti GBA antibody ab55080) that you use our anti-GAPDH antibodies.

We do have one anti-GAPDH product which is guaranteed to work with E.coli, Ab85760. Further information about this product may be found at the following page:

https://www.abcam.com/GAPDH-antibody-HRP-ab85760.html


I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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Thank you for contacting us. I am sorry to hear that ab55080 is not providing satisfactory results in WB.  Having reviewed this case, I would like to offer some suggestions to help optimize your results.  I would also appreciate if you can confirm some further details: 1.)  When you say that the detection was done with AP, did it involve ECL reagents or was the detection colorometric?  We find ECL to be much more sensitive than colorometric detection, and we use ECL for our in house WB validation.  2.)  It may help to use a lower percentage of milk in your antibody diluent.  I typically recommend 0.1 - 1 % milk in the antibody diluent because higher concentrations can prevent the antibody from binding effectively to the protein of interest.  3.)  What secondary antibody was used and at what dilution?  For weak signal, it can sometimes help to use more secondary antibody.  Should the suggestions not improve the results, please do let me know. In the event that a product is not functioning in the species and applications cited on the product datasheet (and the problem has been reported within 6 months of purchase), we would be pleased to provide a free of charge replacement, credit note, or refund. I hope this information is helpful, and I thank you for your cooperation.  

Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products. I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement with the order number 949227. To check the status of the order please contact our Customer Service team and reference this number. Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know. I wish you the best of luck with your research. PS: As discussed please try adding 50% Glycerol or 3% BSA before freezing these antibodies.