Recombinant Anti-GBP1 antibody [EPR8285] (ab131255)
![Western blot - Anti-GBP1 antibody [EPR8285] (ab131255)](https://www.abcam.com/ps/products/131/ab131255/Images/ab131255-397688-anti-gbp1-antibody-epr8285-western-blot-human-knockout-lysate.jpg "Western blot - Anti-GBP1 antibody [EPR8285] (ab131255)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR8285] to GBP1
- Suitable for: WB, IHC-P, ICC/IF
- Knockout validated
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-GBP1 antibody [EPR8285]
See all GBP1 primary antibodies -
Description
Rabbit monoclonal [EPR8285] to GBP1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details
Unsuitable for: Flow Cyt or IP -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human GBP1 aa 550-650. The exact sequence is proprietary.
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Positive control
- WB: A549 and HeLa (with and without treatment with IFN gamma) cell lysates. IHC-P: Human spleen tissue. ICC/IF: IFN gamma induced HeLa cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Dissociation constant (KD)
KD = 2.00 x 10 -12 M Learn more about KD -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR8285 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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KO cell lines
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KO cell lysates
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab131255 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/1000 - 1/10000. Detects a band of approximately 68 kDa (predicted molecular weight: 68 kDa).
|
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| IHC-P |
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| ICC/IF |
1/100 - 1/250.
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| Notes |
|---|
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WB
1/1000 - 1/10000. Detects a band of approximately 68 kDa (predicted molecular weight: 68 kDa). |
|
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
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ICC/IF
1/100 - 1/250. |
Target
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Function
Binds GTP, GDP and GMP. -
Sequence similarities
Belongs to the GBP family. -
Cellular localization
Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 2633 Human
- Omim: 600411 Human
- SwissProt: P32455 Human
- Unigene: 62661 Human
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Alternative names
- GBP 1 antibody
- GBP-1 antibody
- GBP1 antibody
see all
Images
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Western blot - Anti-GBP1 antibody [EPR8285] (ab131255)All lanes : Anti-GBP1 antibody [EPR8285] (ab131255) at 1/1000 dilution
Lane 1 : Wild-type A549 cell lysate
Lane 2 : GBP1 knockout A549 cell lysate
Lane 3 : HeLa treated with 10ng/ml IFN-? for 24 hours, whole cell lysate
Lane 4 : Untreated HeLa cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution
Predicted band size: 68 kDa
Observed band size: 68 kDaLanes 1-4: Merged signal (red and green). Green - ab131255 observed at 68 kDa. Red - loading control ab8245 observed at 36 kDa.
ab131255 Anti-GBP1 antibody [EPR8285] was shown to specifically react with GBP1 in wild-type A549 cells. Loss of signal was observed when knockout cell line ab267202 (knockout cell lysate ab257960) was used. Wild-type and GBP1 knockout samples were subjected to SDS-PAGE. ab131255 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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![Immunocytochemistry/ Immunofluorescence - Anti-GBP1 antibody [EPR8285] (ab131255)](https://www.abcam.com/ps/products/131/ab131255/Images/ab131255-145583-anti-gbp1-antibody-epr8285-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-GBP1 antibody [EPR8285] (ab131255)Immunofluorescent analysis of IFN-gamma induced HeLa cells labelling GBP1 with ab131255 antibody at 1/100 dilution. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GBP1 antibody [EPR8285] (ab131255)](https://www.abcam.com/ps/products/131/ab131255/Images/ab131255-145582-anti-gbp1-antibody-epr8285-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GBP1 antibody [EPR8285] (ab131255)Immunohistochemical analysis of paraffin embedded Human spleen tissue labelling GBP1 with ab131255 antibody at 1/50 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Western blot - Anti-GBP1 antibody [EPR8285] (ab131255)](https://www.abcam.com/ps/products/131/ab131255/Images/ab131255-145584-anti-gbp1-antibody-epr8285-western-blot.jpg)
Western blot - Anti-GBP1 antibody [EPR8285] (ab131255)All lanes : Anti-GBP1 antibody [EPR8285] (ab131255) at 1/1000 dilution
Lane 1 : HeLa cell lysate, treated with IFN gamma
Lane 2 : HeLa cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled Goat anti Rabbit IgG at 1/2000 dilution
Predicted band size: 68 kDa -
![OI-RD Scanning - Anti-GBP1 antibody [EPR8285] (ab131255)](https://www.abcam.com/ps/products/131/ab131255/Images/ab131255-145581-anti-gbp1-antibody-epr8285.jpg)
OI-RD Scanning - Anti-GBP1 antibody [EPR8285] (ab131255) -
![Anti-GBP1 antibody [EPR8285] (ab131255)](https://www.abcam.com/ps/products/131/ab131255/Images/ab131255-6-benefits-of-recombinant-antibodies.png)
Anti-GBP1 antibody [EPR8285] (ab131255)
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (6)
ab131255 has been referenced in 6 publications.
- Kutsch M et al. Direct binding of polymeric GBP1 to LPS disrupts bacterial cell envelope functions. EMBO J 39:e104926 (2020). PubMed: 32510692
- Wandel MP et al. Guanylate-binding proteins convert cytosolic bacteria into caspase-4 signaling platforms. Nat Immunol 21:880-891 (2020). PubMed: 32541830
- Zhao J et al. Oncogenic Role of Guanylate Binding Protein 1 in Human Prostate Cancer. Front Oncol 9:1494 (2019). PubMed: 31998647
- Halu A et al. Context-enriched interactome powered by proteomics helps the identification of novel regulators of macrophage activation. Elife 7:N/A (2018). PubMed: 30303482
- Piro AS et al. Detection of CytosolicShigella flexnerivia a C-Terminal Triple-Arginine Motif of GBP1 Inhibits Actin-Based Motility. MBio 8:N/A (2017). PubMed: 29233899
- Zou Z et al. Guanylate-Binding Protein 1 Inhibits Nuclear Delivery of Kaposi's Sarcoma-Associated Herpesvirus Virions by Disrupting Formation of Actin Filament. J Virol 91:N/A (2017). PubMed: 28592529