Anti-GCDH/GCD antibody [3E9BA3BF5] (ab112998)
![Immunocytochemistry/ Immunofluorescence - Anti-GCDH/GCD antibody [3E9BA3BF5] (ab112998)](https://www.abcam.com/ps/products/112/ab112998/Images/GCDH-Primary-antibodies-ab112998-1.jpg "Immunocytochemistry/ Immunofluorescence - Anti-GCDH/GCD antibody [3E9BA3BF5] (ab112998)")
Key features and details
- Mouse monoclonal [3E9BA3BF5] to GCDH/GCD
- Suitable for: ICC/IF, Flow Cyt
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-GCDH/GCD antibody [3E9BA3BF5]
See all GCDH/GCD primary antibodies -
Description
Mouse monoclonal [3E9BA3BF5] to GCDH/GCD -
Host species
Mouse -
Tested applications
Suitable for: ICC/IF, Flow Cytmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant full length protein. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Fibroblast, HeLa, HepG2, and Rat Cardiomyocyte cells.
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General notes
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Product was previously marketed under the MitoSciences sub-brand.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. -
Storage buffer
pH: 7.5
Preservative: 0.02% Sodium azide
Constituent: 99.98% HEPES buffered saline -
Concentration information loading... -
Purity
Proprietary Purification -
Purification notes
ab112998 was produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation. Monoclonal purity was near homogeneity as judged by SDS-PAGE (>95%). -
Clonality
Monoclonal -
Clone number
3E9BA3BF5 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab112998 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF |
Use a concentration of 1 µg/ml.
|
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| Flow Cyt |
Use a concentration of 1 µg/ml.
0.1% Triton X-100 permeabilization recommended. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
| Notes |
|---|
|
ICC/IF
Use a concentration of 1 µg/ml. |
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Flow Cyt
Use a concentration of 1 µg/ml. 0.1% Triton X-100 permeabilization recommended. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
Target
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Function
Catalyzes the oxidative decarboxylation of glutaryl-CoA to crotonyl-CoA and CO(2) in the degradative pathway of L-lysine, L-hydroxylysine, and L-tryptophan metabolism. It uses electron transfer flavoprotein as its electron acceptor. Isoform Short is inactive. -
Tissue specificity
Isoform 1 and isoform 2 are expressed in fibroblasts and liver. -
Pathway
Amino-acid metabolism; lysine degradation.
Amino-acid metabolism; tryptophan metabolism. -
Involvement in disease
Defects in GCDH are the cause of glutaric aciduria type 1 (GA1) [MIM:231670]. GA1 is an autosomal recessive metabolic disorder characterized by progressive dystonia and athetosis due to gliosis and neuronal loss in the basal ganglia. -
Sequence similarities
Belongs to the acyl-CoA dehydrogenase family. -
Cellular localization
Mitochondrion matrix. - Information by UniProt
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Database links
- Entrez Gene: 2639 Human
- Omim: 608801 Human
- SwissProt: Q92947 Human
- Unigene: 532699 Human
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Alternative names
- ACAD5 antibody
- EC 1.3.99.7 antibody
- GCD antibody
see all
Images
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Immunocytochemistry/ Immunofluorescence - Anti-GCDH/GCD antibody [3E9BA3BF5] (ab112998)
ab112998 at 1 µg/ml staining GCDH/GCD in fibroblast cells by immunocytochemistry (4% paraformaldehyde fixed and 0.1% Triton X-100 permeabilized) followed by Alexa Fluor® 594 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1 hour (red). Nuclei were stained with DAPI.
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![Flow Cytometry - Anti-GCDH/GCD antibody [3E9BA3BF5] (ab112998)](https://www.abcam.com/ps/products/112/ab112998/Images/GCDH-Primary-antibodies-ab112998-2.jpg)
Flow Cytometry - Anti-GCDH/GCD antibody [3E9BA3BF5] (ab112998)ab112998 at 1ug/ml staining GCDH/GCD in HeLa cells by Flow Cytometry (blue). Isotype control antibody (red).
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab112998 has not yet been referenced specifically in any publications.