Recombinant Anti-GDF 9 antibody [EPR22495-129] (ab254323)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GDF 9 antibody [EPR22495-129] (ab254323)](https://www.abcam.com/ps/products/254/ab254323/Images/ab254323-2-anti-gdf9-antibody-epr22495-129-immunohistochemistry-ovary-human.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GDF 9 antibody [EPR22495-129] (ab254323)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR22495-129] to GDF 9
- Suitable for: IHC-P, IHC-Fr
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-GDF 9 antibody [EPR22495-129]
See all GDF 9 primary antibodies -
Description
Rabbit monoclonal [EPR22495-129] to GDF 9 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, IHC-Frmore details
Unsuitable for: Flow Cyt,ICC/IF,IP or WB -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- IHC-P: Human, mouse and rat ovary tissue. IHC-Fr: Mouse ovary tissue.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 0.05% BSA, 40% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR22495-129 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Immunohistochemistry kits
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab254323 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | (1) |
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Use at 1/500 dilution for human, 1/2000 for mouse and rat. |
| IHC-Fr |
1/50.
Weak reactivity in rat.Heat mediated antigen retrieval using sodium citrate buffer (10 mM citrate pH 6.0 and 0.05% Tween-20). |
| Notes |
|---|
|
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. Use at 1/500 dilution for human, 1/2000 for mouse and rat. |
|
IHC-Fr
1/50. Weak reactivity in rat.Heat mediated antigen retrieval using sodium citrate buffer (10 mM citrate pH 6.0 and 0.05% Tween-20). |
Target
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Relevance
GDF 9 is a member of the bone morphogenetic protein (BMP) family and the TGF-beta superfamily. This group of proteins is characterized by a polybasic proteolytic processing site which is cleaved to produce a mature protein containing seven conserved cysteine residues. The members of this family are regulators of cell growth and differentiation in both embryonic and adult tissues. Growth factors synthesized by ovarian somatic cells directly affect oocyte growth and function. GDF 9 is expressed in oocytes and is thought to be required for ovarian folliculogenesis. -
Cellular localization
Secreted -
Database links
- Entrez Gene: 2661 Human
- Entrez Gene: 14566 Mouse
- Entrez Gene: 59304 Rat
- Omim: 601918 Human
- SwissProt: O60383 Human
- SwissProt: Q07105 Mouse
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Alternative names
- GDF9 antibody
- Growth differentiation factor 9 antibody
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GDF 9 antibody [EPR22495-129] (ab254323)
Immunohistochemical analysis of paraffin-embedded human ovary tissue labeling GDF 9 with ab254323 at 1/500 dilution, followed by ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on human ovarian follicles (PMID: 21401961). The section was incubated with ab254323 for 30 mins at RT. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.
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![Immunohistochemistry (Frozen sections) - Anti-GDF 9 antibody [EPR22495-129] (ab254323)](https://www.abcam.com/ps/products/254/ab254323/Images/ab254323-1-anti-gdf9-antibody-epr22495-129-immunohistochemistry-frozen-ovary-mouse.jpg)
Immunohistochemistry (Frozen sections) - Anti-GDF 9 antibody [EPR22495-129] (ab254323)Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen mouse ovary tissue labeling GDF 9 with ab254323 at 1/50 dilution (green), followed by ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at a 1/1,000 dilution. Positive staining on follicles of mouse ovary (PMID: 21401961) is observed. Counterstained with DAPI (blue).
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab150077 AlexaFluor®488 Goat anti-Rabbit used at a 1/1,000 dilution.
Heat mediated antigen retrieval using sodium citrate buffer (10 mM citrate pH 6.0 and 0.05% Tween-20).
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GDF 9 antibody [EPR22495-129] (ab254323)](https://www.abcam.com/ps/products/254/ab254323/Images/ab254323-3-anti-gdf9-antibody-epr22495-129-immunohistochemistry-ovary-mouse.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GDF 9 antibody [EPR22495-129] (ab254323)Immunohistochemical analysis of paraffin-embedded mouse ovary tissue labeling GDF 9 with ab254323 at 1/2000 dilution, followed by ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on mouse ovarian follicles (PMID: 21401961). The section was incubated with ab254323 for 30 mins at RT. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GDF 9 antibody [EPR22495-129] (ab254323)](https://www.abcam.com/ps/products/254/ab254323/Images/ab254323-4-anti-gdf9-antibody-epr22495-129-immunohistochemistry-ovary-rat.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GDF 9 antibody [EPR22495-129] (ab254323)Immunohistochemical analysis of paraffin-embedded rat ovary tissue labeling GDF 9 with ab254323 at 1/2000 dilution, followed by ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on rat ovarian follicles (PMID: 21401961). The section was incubated with ab254323 for 30 mins at RT. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GDF 9 antibody [EPR22495-129] (ab254323)](https://www.abcam.com/ps/products/254/ab254323/Images/ab254323-5-anti-gdf9-antibody-epr22495-129-immunohistochemistry-spleen-mouse.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GDF 9 antibody [EPR22495-129] (ab254323)Immunohistochemical analysis of paraffin-embedded mouse spleen tissue labeling GDF 9 with ab254323 at 1/2000 dilution, followed by ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Negative control: No staining on mouse spleen (PMID: 22732078). The section was incubated with ab254323 for 30 mins at RT. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.
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![Anti-GDF 9 antibody [EPR22495-129] (ab254323)](https://www.abcam.com/ps/products/254/ab254323/Images/ab254323-6-benefits-of-recombinant-antibodies.png)
Anti-GDF 9 antibody [EPR22495-129] (ab254323)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (0)
ab254323 has not yet been referenced specifically in any publications.