Recombinant Anti-GDF11 + GDF8/Myostatin antibody [EPR4567(2)] (ab124721)
![Western blot - Anti-GDF11 + GDF8/Myostatin antibody [EPR4567(2)] (ab124721)](https://www.abcam.com/ps/products/124/ab124721/Images/ab124721-505679-anti-gdf11-gdf8-myostatin-antibody-epr4567-2-western-blot-human-mouse-rat-tissues.jpg "Western blot - Anti-GDF11 + GDF8/Myostatin antibody [EPR4567(2)] (ab124721)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR4567(2)] to GDF11 + GDF8/Myostatin
- Suitable for: Flow Cyt (Intra), ICC/IF, WB, IHC-P
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-GDF11 + GDF8/Myostatin antibody [EPR4567(2)]
See all GDF11 + GDF8/Myostatin primary antibodies -
Description
Rabbit monoclonal [EPR4567(2)] to GDF11 + GDF8/Myostatin -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt (Intra), ICC/IF, WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide within Human GDF11 + GDF8/Myostatin aa 350 to the C-terminus. The exact sequence is proprietary.
Database link: O95390 -
Positive control
- WB: HeLa, HEK293 and C6 cell lysate and human heart tissue lysate. C2C12 cell lysate. IHC-P: Human colon adenocarcinoma and cerebral cortex tissues. ICC/IF: A549 cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. -
Dissociation constant (KD)
KD = 6.40 x 10 -11 M Learn more about KD -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 0.05% BSA, 59% PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR4567(2) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Positive Controls
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab124721 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt (Intra) |
Use at an assay dependent concentration.
Purified format. |
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| ICC/IF |
1/250.
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| WB | (1) |
1/1000. Detects a band of approximately 45 kDa (predicted molecular weight: 45 kDa).
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| IHC-P | (4) |
1/1000 - 1/2500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
See IHC antigen retrieval protocols. For unpurified use at 1/500 - 1/1000. |
| Notes |
|---|
|
Flow Cyt (Intra)
Use at an assay dependent concentration. Purified format. |
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ICC/IF
1/250. |
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WB
1/1000. Detects a band of approximately 45 kDa (predicted molecular weight: 45 kDa). |
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IHC-P
1/1000 - 1/2500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. See IHC antigen retrieval protocols. For unpurified use at 1/500 - 1/1000. |
Target
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Relevance
O95390: Function - Secreted signal that acts globally to specify positional identity along the anterior/posterior axis during development. Play critical roles in patterning both mesodermal and neural tissues and in establishing the skeletal pattern. O14793: Function - Acts specifically as a negative regulator of skeletal muscle growth. -
Database links
- Entrez Gene: 10220 Human
- Entrez Gene: 2660 Human
- Entrez Gene: 14561 Mouse
- Entrez Gene: 17700 Mouse
- Entrez Gene: 29152 Rat
- Entrez Gene: 29454 Rat
- SwissProt: O14793 Human
- SwissProt: O95390 Human
see all -
Alternative names
- BMP-11 antibody
- BMP11 antibody
- Bone morphogenetic protein 11 antibody
see all
Images
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Western blot - Anti-GDF11 + GDF8/Myostatin antibody [EPR4567(2)] (ab124721)All lanes : Anti-GDF11 + GDF8/Myostatin antibody [EPR4567(2)] (ab124721) at 1/1000 dilution
Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate
Lane 2 : HEK-293 (Human embryonic kidney epithelial cell) whole cell lysate
Lane 3 : C2C12 (Mouse myoblasts myoblast) whole cell lysate
Lane 4 : C6 (Rat glial tumor glial cell) whole cell lysate
Lane 5 : Rat liver lysate
Lane 6 : Rat heart lysate
Lane 7 : Rat kidney lysate
Lane 8 : Mouse brain lysate
Lane 9 : Mouse liver lysate
Lane 10 : Human liver lysate
Lane 11 : Human skeletal muscle lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Predicted band size: 45 kDa
Observed band size: 45 kDa
Exposure time: 180 secondsBlocking and diluting buffer: 5% NFDM/TBST
This antibody is unsuitable to detect tissue samples.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GDF11 + GDF8/Myostatin antibody [EPR4567(2)] (ab124721)](https://www.abcam.com/ps/products/124/ab124721/Images/ab124721-138535-anti-bmp11-gdf8-myostatin-antibody-epr45672-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GDF11 + GDF8/Myostatin antibody [EPR4567(2)] (ab124721)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colon adenocarcinoma tissue labelling BMP11 + GDF8/Myostatin with unpurified ab124721 at 1/500 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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![Flow Cytometry (Intracellular) - Anti-GDF11 + GDF8/Myostatin antibody [EPR4567(2)] (ab124721)](https://www.abcam.com/ps/products/124/ab124721/Images/ab124721-271162-anti-bmp11-gdf8-myostatin-antibody-epr45672-flow-cytometry.jpg)
Flow Cytometry (Intracellular) - Anti-GDF11 + GDF8/Myostatin antibody [EPR4567(2)] (ab124721)ab124721 staining GDF11 + GDF8/Myostatin in the human cell line HeLa (human cervix adenocarcinoma) by intracellular flow cytometry. Cells were fixed with 4% paraformaldehyde, permabilised with 90% methanol and the sample was incubated with the primary antibody at a dilution of 1/250. A goat anti rabbit IgG (Alexa Fluor® 488) at a dilution of 1/2000 was used as the secondary antibody.
Isoytype control: Rabbit monoclonal IgG (Black)
Unlabelled control: Cell without incubation with primary antibody and secondary antibody (Blue)
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GDF11 + GDF8/Myostatin antibody [EPR4567(2)] (ab124721)](https://www.abcam.com/ps/products/124/ab124721/Images/ab124721-240333-anti-bmp11-gdf8-myostatin-antibody-epr45672-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GDF11 + GDF8/Myostatin antibody [EPR4567(2)] (ab124721)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human cerebral cortex tissue labelling BMP11 + GDF8/Myostatin with purified ab124721 at 1/2500. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. A prediluted HRP-polymer conjugated anti-rabbit IgG was used as the secondary antibody. Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.
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![Immunocytochemistry/ Immunofluorescence - Anti-GDF11 + GDF8/Myostatin antibody [EPR4567(2)] (ab124721)](https://www.abcam.com/ps/products/124/ab124721/Images/ab124721-240334-anti-bmp11-gdf8-myostatin-antibody-epr45672-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-GDF11 + GDF8/Myostatin antibody [EPR4567(2)] (ab124721)Immunocytochemistry/Immunofluorescence analysis of A549 cells labelling BMP11 + GDF8/Myostatin with purified ab124721 at 1/250. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% triton X-100. ab150078, an Alexa Fluor® 555-conjugated goat anti-rabbit IgG (1/500) was used as the secondary antibody. DAPI (blue) was used as the nuclear counterstain.
Control: primary antibody (1/250) and secondary antibody, ab150113, an Alexa Fluor® 488-conjugated goat anti-mouse IgG (1/500).
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![OI-RD Scanning - Anti-GDF11 + GDF8/Myostatin antibody [EPR4567(2)] (ab124721)](https://www.abcam.com/ps/products/124/ab124721/Images/ab124721-138534-anti-bmp11-gdf8-myostatin-antibody-epr45672.jpg)
OI-RD Scanning - Anti-GDF11 + GDF8/Myostatin antibody [EPR4567(2)] (ab124721) -
![Anti-GDF11 + GDF8/Myostatin antibody [EPR4567(2)] (ab124721)](https://www.abcam.com/ps/products/124/ab124721/Images/ab124721-11-benefits-of-recombinant-antibodies.png)
Anti-GDF11 + GDF8/Myostatin antibody [EPR4567(2)] (ab124721)
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (30)
ab124721 has been referenced in 30 publications.
- Kim GT et al. PLAG alleviates cisplatin-induced cachexia in lung cancer implanted mice. Transl Oncol 20:101398 (2022). PubMed: 35339890
- Ge L et al. Myostatin site-directed mutation and simultaneous PPAR? site-directed knockin in bovine genome. J Cell Physiol 236:2592-2605 (2021). PubMed: 32841375
- Liu L et al. Formononetin ameliorates muscle atrophy by regulating myostatin-mediated PI3K/Akt/FoxO3a pathway and satellite cell function in chronic kidney disease. J Cell Mol Med 25:1493-1506 (2021). PubMed: 33405354
- Sun L et al. Molecular and Metabolic Mechanism of Low-Intensity Pulsed Ultrasound Improving Muscle Atrophy in Hindlimb Unloading Rats. Int J Mol Sci 22:N/A (2021). PubMed: 34829990
- Lin J et al. The GDF11 Promotes Nerve Regeneration After Sciatic Nerve Injury in Adult Rats by Promoting Axon Growth and Inhibiting Neuronal Apoptosis. Front Bioeng Biotechnol 9:803052 (2021). PubMed: 35059389