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Anti-GDF15 antibody [7C12.B3.F2] (ab106112)

Reviews (1) Submit a question References (1)
Immunocytochemistry/ Immunofluorescence - Anti-GDF15 antibody [7C12.B3.F2] (ab106112)
  • Western blot - Anti-GDF15 antibody [7C12.B3.F2] (ab106112)

Key features and details

  • Mouse monoclonal [7C12.B3.F2] to GDF15
  • Suitable for: WB, ICC/IF
  • Reacts with: Human
  • Isotype: unknown

Overview

  • Product name

    Anti-GDF15 antibody [7C12.B3.F2]
    See all GDF15 primary antibodies

  • Description

    Mouse monoclonal [7C12.B3.F2] to GDF15

  • Host species

    Mouse

  • Specificity

    ab106112 specifically reacts with an H variant sequence of GDF15 protein.

  • Tested applications

    Suitable for: WB, ICC/IFmore details

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Chimpanzee, Macaque monkey

  • Immunogen

    A synthetic peptide corresponding to a region near the amino terminal end of Human GDF15 protein. A residue of cysteine was added to facilitate coupling to KLH.

  • Positive control

    • Pichia pastoris (yeast) whole cell lysate expressing recombinant GDF15 H variant with SUMO expression tag.

  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab106112 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/1000. Predicted molecular weight: 34 kDa.
ICC/IF (1)
1/1000.
Notes
WB
1/1000. Predicted molecular weight: 34 kDa.
ICC/IF
1/1000.

Target

  • Tissue specificity

    Highly expressed in placenta, with lower levels in prostate and colon and some expression in kidney.

  • Sequence similarities

    Belongs to the TGF-beta family.

  • Cellular localization

    Secreted.
  • Information by UniProt

  • Database links

    • Alternative names

      • GDF 15 antibody
      • GDF-15 antibody
      • Gdf15 antibody
      • GDF15_HUMAN antibody
      • Growth differentiation factor 15 antibody
      • Growth/differentiation factor 15 antibody
      • Macrophage inhibitory cytokine 1 antibody
      • MIC 1 antibody
      • Mic-1 antibody
      • MIC1 antibody
      • NAG 1 antibody
      • NAG-1 antibody
      • NAG1 antibody
      • NRG 1 antibody
      • NRG-1 antibody
      • NRG1 antibody
      • NSAID (nonsteroidal anti inflammatory drug) activated protein 1 antibody
      • NSAID antibody
      • NSAID regulated protein 1 antibody
      • NSAID-activated gene 1 protein antibody
      • NSAID-regulated gene 1 protein antibody
      • PDF antibody
      • PLAB antibody
      • Placental bone morphogenetic protein antibody
      • Placental bone morphogenic protein antibody
      • Placental TGF beta antibody
      • Placental TGF-beta antibody
      • Prostate differentiation factor antibody
      • PTGF beta antibody
      • PTGFB antibody
      see all

    Images

    • Immunocytochemistry/ Immunofluorescence - Anti-GDF15 antibody [7C12.B3.F2] (ab106112)
      Immunocytochemistry/ Immunofluorescence - Anti-GDF15 antibody [7C12.B3.F2] (ab106112)This image is courtesy of Dr. Ruma Raha-Chowdhury (Cambridge University, United Kingdom)

      ICC/IF image of GDF15 staining on human blood film using ab106112 (1:1000). The cells were fixed using formaldehyde and permeabilized using 0.1% TritonX in 0.1% PBS. The cells were then blocked using 10% donkey serum for 1 hour at 24°C. ab106112 was diluted to 1:1000 and incubated with cells for 4 hours at 24°C. The secondary antibody used was donkey polyclonal to mouse IgG conjugated to Alexa Fluor 568.

    • Western blot - Anti-GDF15 antibody [7C12.B3.F2] (ab106112)
      Western blot - Anti-GDF15 antibody [7C12.B3.F2] (ab106112)
      All lanes : Anti-GDF15 antibody [7C12.B3.F2] (ab106112) at 1/1000 dilution

      Lane 1 : Pichia pastoris (yeast) whole cell lysate expressing recombinant GDF15 H variant with SUMO expression tag
      Lane 2 : Pichia pastoris (yeast) whole cell lysate expressing recombinant GDF15 D variant with SUMO expression tag
      Lane 3 : Pichia pastoris (yeast) whole cell lysate expressing recombinant GDF15 H variant
      Lane 4 : Pichia pastoris (yeast) whole cell lysate expressing recombinant GDF15 D variant

      Secondary
      All lanes : Peroxidase conjugated Gt-a-Mouse IgG at 1/40000 dilution

      Performed under reducing conditions.

      Predicted band size: 34 kDa

    References (1)

    Publishing research using ab106112? Please let us know so that we can cite the reference in this datasheet.

    ab106112 has been referenced in 1 publication.

    • Addison R  et al. Therapeutic wavelengths of ultraviolet B radiation activate apoptotic, circadian rhythm, redox signalling and key canonical pathways in psoriatic epidermis. Redox Biol 41:101924 (2021). PubMed: 33812333

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    Immunocytochemistry/ Immunofluorescence abreview for Anti-GDF15 antibody [7C12.B3.F2]

     Excellent
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 24°C
    Sample
    Human Cell (Human blood film)
    Specification
    Human blood film
    Permeabilization
    Yes - 0.1% TritonX in 0.1% PBS
    Fixative
    Formaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Dr. Ruma Raha-Chowdhury

    Verified customer

    Submitted Sep 20 2013

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