Recombinant Anti-GDF15 antibody [EPR19939] (ab206414)
![Western blot - Anti-GDF15 antibody [EPR19939] (ab206414)](https://www.abcam.com/ps/products/206/ab206414/Images/ab206414-267029-anti-gdf15-antibody-epr19939-western-blot.jpg "Western blot - Anti-GDF15 antibody [EPR19939] (ab206414)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR19939] to GDF15
- Suitable for: IHC-P, WB, IP, ELISA
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-GDF15 antibody [EPR19939]
See all GDF15 primary antibodies -
Description
Rabbit monoclonal [EPR19939] to GDF15 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WB, IP, ELISAmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: HepG2 and LNCaP whole cell lysates; human prostate cancer and placenta lysates; untreated HT1080 and treated with 200ng/ml Phorbol-12-myristate-13-acetate (TPA) for 24 hours whole cell lysates. IHC-P: Human placenta and prostate hyperplasia tissues. IP: HepG2 whole cell lysate.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR19939 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab206414 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P |
1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
IHC tests show positive staining only on human placenta and prostate hyperplasia tissues, other tissues tested were negative. |
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| WB |
1/1000. Detects a band of approximately 35, 12.5 kDa (predicted molecular weight: 34 kDa).
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| IP |
1/30.
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| ELISA |
Use at an assay dependent concentration.
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| Notes |
|---|
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IHC-P
1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. IHC tests show positive staining only on human placenta and prostate hyperplasia tissues, other tissues tested were negative. |
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WB
1/1000. Detects a band of approximately 35, 12.5 kDa (predicted molecular weight: 34 kDa). |
|
IP
1/30. |
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ELISA
Use at an assay dependent concentration. |
Target
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Tissue specificity
Highly expressed in placenta, with lower levels in prostate and colon and some expression in kidney. -
Sequence similarities
Belongs to the TGF-beta family. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 9518 Human
- Omim: 605312 Human
- SwissProt: Q99988 Human
- Unigene: 616962 Human
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Alternative names
- GDF 15 antibody
- GDF-15 antibody
- Gdf15 antibody
see all
Images
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Western blot - Anti-GDF15 antibody [EPR19939] (ab206414)All lanes : Anti-GDF15 antibody [EPR19939] (ab206414) at 1/1000 dilution
Lane 1 : HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate
Lane 2 : LNCaP (Human prostate cancer cell line) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution
Predicted band size: 34 kDa
Observed band size: 12.5,35 kDa why is the actual band size different from the predicted?Blocking/Dilution buffer: 5% NFDM/TBST.
Exposure time: Lane 1: 3 minutes; Lane 2: 10 seconds.
The molecular weight observed is consistent with the literature (PMID: 20884666).
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GDF15 antibody [EPR19939] (ab206414)](https://www.abcam.com/ps/products/206/ab206414/Images/ab206414-267024-anti-gdf15-antibody-epr19939-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GDF15 antibody [EPR19939] (ab206414)Immunohistochemical analysis of paraffin-embedded human placenta tissue labeling GDF15 with ab206414 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
Cytoplasmic staining on human placenta is observed (PMID: 9593718). Positive staining on human placenta and prostate hyperplasia, other tissues tested were negative.
Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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![Western blot - Anti-GDF15 antibody [EPR19939] (ab206414)](https://www.abcam.com/ps/products/206/ab206414/Images/ab206414-267028-anti-gdf15-antibody-epr19939-western-blot.jpg)
Western blot - Anti-GDF15 antibody [EPR19939] (ab206414)All lanes : Anti-GDF15 antibody [EPR19939] (ab206414) at 1/1000 dilution
Lane 1 : Human prostate cancer lysate
Lane 2 : Human placenta lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution
Predicted band size: 34 kDa
Observed band size: 12.5 kDa why is the actual band size different from the predicted?Blocking/Dilution buffer: 5% NFDM/TBST.
Exposure time: Lane 1: 10 seconds; Lane 2: 3 minutes.
The molecular weight observed is consistent with the literature (PMID: 20884666).
-
![Western blot - Anti-GDF15 antibody [EPR19939] (ab206414)](https://www.abcam.com/ps/products/206/ab206414/Images/ab206414-267026-anti-gdf15-antibody-epr19939-western-blot.jpg)
Western blot - Anti-GDF15 antibody [EPR19939] (ab206414)All lanes : Anti-GDF15 antibody [EPR19939] (ab206414) at 1/1000 dilution
Lane 1 : Untreated HT1080 (Human fibrosarcoma cell line) whole cell lysate
Lane 2 : HT1080 (Human fibrosarcoma cell line) whole cell lysate treated with 200ng/ml Phorbol-12-myristate-13-acetate (TPA) for 24 hours
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 34 kDa
Observed band size: 35 kDa why is the actual band size different from the predicted?
Exposure time: 3 secondsBlocking/Dilution buffer: 5% NFDM/TBST.
The expression level of GDF15 can be increased by TPA treatment (PMID: 15757899).
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GDF15 antibody [EPR19939] (ab206414)](https://www.abcam.com/ps/products/206/ab206414/Images/ab206414-267023-anti-gdf15-antibody-epr19939-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GDF15 antibody [EPR19939] (ab206414)Immunohistochemical analysis of paraffin-embedded human prostate hyperplasia tissue labeling GDF15 with ab206414 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
Cytoplasmic staining on human prostate hyperplasia is observed (PMID: 9593718). Positive staining on human placenta and prostate hyperplasia, other tissues tested were negative.
Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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![Immunoprecipitation - Anti-GDF15 antibody [EPR19939] (ab206414)](https://www.abcam.com/ps/products/206/ab206414/Images/ab206414-267022-anti-gdf15-antibody-epr19939-immunoprecipitation.jpg)
Immunoprecipitation - Anti-GDF15 antibody [EPR19939] (ab206414)GDF15 was immunoprecipitated from 0.35 mg of HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate with ab206414 at 1/30 dilution.
Western blot was performed from the immunoprecipitate using ab206414 at 1/1000 dilution.
VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution.
Lane 1: HepG2 whole cell lysate 10µg (Input).
Lane 2: ab206414 IP in HepG2 whole cell lysate.
Lane 3: Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) instead of ab206414 in HepG2 whole cell lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 10 seconds.
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![ELISA - Anti-GDF15 antibody [EPR19939] (ab206414)](https://www.abcam.com/ps/products/206/ab206414/Images/ab206414-400194-anti-gdf15-antibody-epr19939-elisa.jpg)
ELISA - Anti-GDF15 antibody [EPR19939] (ab206414)ELISA analysis of ab206414 binding functionally active mature GDF15 (ab50077) at 1 µg/mL.
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![Western blot - Anti-GDF15 antibody [EPR19939] (ab206414)](https://www.abcam.com/ps/products/206/ab206414/Images/ab206414-267025-anti-gdf15-antibody-epr19939-western-blot.jpg)
Western blot - Anti-GDF15 antibody [EPR19939] (ab206414)All lanes : Anti-GDF15 antibody [EPR19939] (ab206414) at 1/1000 dilution
Lane 1 : Untreated HT1080 (Human fibrosarcoma cell line) whole cell lysate
Lane 2 : HT1080 (Human fibrosarcoma cell line) whole cell lysate treated with 200ng/ml Phorbol-12-myristate-13-acetate (TPA) for 24 hours
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 34 kDa
Observed band size: 35 kDa why is the actual band size different from the predicted?
Exposure time: 1 minuteBlocking/Dilution buffer: 5% NFDM/TBST.
Both pro and mature form can be increased by TPA treatment.
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![Anti-GDF15 antibody [EPR19939] (ab206414)](https://www.abcam.com/ps/products/206/ab206414/Images/ab206414-10-benefits-of-recombinant-antibodies.png)
Anti-GDF15 antibody [EPR19939] (ab206414)
Protocols
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (7)
ab206414 has been referenced in 7 publications.
- Yang S et al. Early pregnancy human decidua gamma/delta T cells exhibit tissue resident and specific functional characteristics. Mol Hum Reprod 28:N/A (2022). PubMed: 35758607
- Wang R et al. N-glycosylation of GDF15 abolishes its inhibitory effect on EGFR in AR inhibitor-resistant prostate cancer cells. Cell Death Dis 13:626 (2022). PubMed: 35853851
- Gao Y et al. Growth differentiation factor-15 promotes immune escape of ovarian cancer via targeting CD44 in dendritic cells. Exp Cell Res 402:112522 (2021). PubMed: 33771482
- Shao Y et al. An Original Ferroptosis-Related Gene Signature Effectively Predicts the Prognosis and Clinical Status for Colorectal Cancer Patients. Front Oncol 11:711776 (2021). PubMed: 34249766
- Duan L et al. The role of GDF15 in bone metastasis of lung adenocarcinoma cells. Oncol Rep 41:2379-2388 (2019). PubMed: 30816507
- Zhang J et al. K-RasG12V/Y40C-PI3K/AKT pathway regulates H1.4S35ph through PKA to promote the occurrence and development of osteosarcoma cancer. Artif Cells Nanomed Biotechnol 47:2048-2057 (2019). PubMed: 31126199
- Jiang G et al. IL-17A and GDF15 are able to induce epithelial-mesenchymal transition of lung epithelial cells in response to cigarette smoke. Exp Ther Med 16:12-20 (2018). PubMed: 29977354