Anti-GDF8 / Myostatin antibody (ab203076)
Key features and details
- Rabbit polyclonal to GDF8 / Myostatin
- Suitable for: WB
- Reacts with: Mouse, Rat
- Isotype: IgG
Overview
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Product name
Anti-GDF8 / Myostatin antibody
See all GDF8 / Myostatin primary antibodies -
Description
Rabbit polyclonal to GDF8 / Myostatin -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Mouse, Rat -
Immunogen
Synthetic peptide within Human GDF8/ Myostatin aa 300 to the C-terminus conjugated to keyhole limpet haemocyanin. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: O14793 -
Positive control
- WB: Mouse muscle lysate, Rat muscle lysate, Rat tongue lysate, Mouse skeletal muscle lysate
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol (glycerin, glycerine), 1% BSA, 48.97% TBS, 1X
Aqueous buffered solution. -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab203076 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/100 - 1/1000. Predicted molecular weight: 43 kDa.
|
| Notes |
|---|
|
WB
1/100 - 1/1000. Predicted molecular weight: 43 kDa. |
Target
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Function
Acts specifically as a negative regulator of skeletal muscle growth. -
Sequence similarities
Belongs to the TGF-beta family. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 17700 Mouse
- Entrez Gene: 29152 Rat
- SwissProt: O08689 Mouse
- SwissProt: O35312 Rat
- Unigene: 3514 Mouse
- Unigene: 44460 Rat
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Alternative names
- GDF 8 antibody
- GDF-8 antibody
- GDF8 antibody
see all
Images
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Western blot - Anti-GDF8 / Myostatin antibody (ab203076)All lanes : Anti-GDF8 / Myostatin antibody (ab203076) at 1/1000 dilution
Lane 1 : Mouse muscle lysate
Lane 2 : Rat muscle lysate
Lane 3 : Rat tongue lysate
Secondary
All lanes : Conjugated secondary at 1/20000 dilution
Predicted band size: 43 kDa -
Western blot - Anti-GDF8 / Myostatin antibody (ab203076)Anti-GDF8 / Myostatin antibody (ab203076) at 1/200 dilution (overnight at 4°C) + Mouse skeletal muscle lysate
Secondary
Goat Anti-Rabbit IgG Antibody (H+L), HRP Conjugated (90 min at 37°C) at 1/3000 dilution
Predicted band size: 43 kDa
Observed band size: 43 kDa
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (15)
ab203076 has been referenced in 15 publications.
- Chi MY et al. Silibinin Alleviates Muscle Atrophy Caused by Oxidative Stress Induced by Cisplatin through ERK/FoxO and JNK/FoxO Pathways. Oxid Med Cell Longev 2022:5694223 (2022). PubMed: 35096269
- Wang R et al. Resveratrol ameliorates muscle atrophy in chronic kidney disease via the axis of SIRT1/FoxO1. Phytother Res 36:3265-3275 (2022). PubMed: 35606908
- Dos Santos M et al. Vitamin D supplementation modulates autophagy in the pristane-induced lupus model. Adv Rheumatol 62:27 (2022). PubMed: 35869515
- Zhang L et al. Myostatin/HIF2α-Mediated Ferroptosis is Involved in Skeletal Muscle Dysfunction in Chronic Obstructive Pulmonary Disease. Int J Chron Obstruct Pulmon Dis 17:2383-2399 (2022). PubMed: 36185172
- Khin PP et al. Dulaglutide improves muscle function by attenuating inflammation through OPA-1-TLR-9 signaling in aged mice. Aging (Albany NY) 13:21962-21974 (2021). PubMed: 34537761