Recombinant Anti-Gelsolin antibody [EPR1942] (ab109014)
![Western blot - Anti-Gelsolin antibody [EPR1942] (ab109014)](https://www.abcam.com/ps/products/109/ab109014/Images/ab109014-469563-anti-gelsolin-antibody-epr1942-western-blot-wildtype-hela-gsn-knockout-hela-human.jpg "Western blot - Anti-Gelsolin antibody [EPR1942] (ab109014)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR1942] to Gelsolin
- Suitable for: WB, IHC-P
- Knockout validated
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-Gelsolin antibody [EPR1942]
See all Gelsolin primary antibodies -
Description
Rabbit monoclonal [EPR1942] to Gelsolin -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details
Unsuitable for: Flow Cyt (Intra),ICC/IF or IP -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- MCF-7, HL-60, A431, and HeLa cell lysates; Human kidney tissue; Human lung tissue; HeLa cells
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR1942 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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KO cell lines
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KO cell lysates
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab109014 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/20000 - 1/50000. Predicted molecular weight: 86 kDa.
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| IHC-P |
1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
| Notes |
|---|
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WB
1/20000 - 1/50000. Predicted molecular weight: 86 kDa. |
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IHC-P
1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
Calcium-regulated, actin-modulating protein that binds to the plus (or barbed) ends of actin monomers or filaments, preventing monomer exchange (end-blocking or capping). It can promote the assembly of monomers into filaments (nucleation) as well as sever filaments already formed. Plays a role in ciliogenesis. -
Tissue specificity
Phagocytic cells, platelets, fibroblasts, nonmuscle cells, smooth and skeletal muscle cells. -
Involvement in disease
Defects in GSN are the cause of amyloidosis type 5 (AMYL5) [MIM:105120]; also known as familial amyloidosis Finnish type. AMYL5 is a hereditary generalized amyloidosis due to gelsolin amyloid deposition. It is typically characterized by cranial neuropathy and lattice corneal dystrophy. Most patients have modest involvement of internal organs, but severe systemic disease can develop in some individuals causing peripheral polyneuropathy, amyloid cardiomyopathy, and nephrotic syndrome leading to renal failure. -
Sequence similarities
Belongs to the villin/gelsolin family.
Contains 6 gelsolin-like repeats. -
Post-translational
modificationsPhosphorylation on Tyr-86, Tyr-409, Tyr-465, Tyr-603 and Tyr-651 in vitro is induced in presence of phospholipids. -
Cellular localization
Cytoplasm > cytoskeleton and Secreted. - Information by UniProt
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Database links
- Entrez Gene: 2934 Human
- Entrez Gene: 227753 Mouse
- Omim: 137350 Human
- SwissProt: P06396 Human
- SwissProt: P13020 Mouse
- Unigene: 522373 Human
- Unigene: 21109 Mouse
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Alternative names
- Actin depolymerizing factor antibody
- Actin-depolymerizing factor antibody
- ADF antibody
see all
Images
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Western blot - Anti-Gelsolin antibody [EPR1942] (ab109014)All lanes : Anti-Gelsolin antibody [EPR1942] (ab109014) at 1/20000 dilution
Lane 1 : Wild-type HeLa cell lysate
Lane 2 : GSN knockout HeLa cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 86 kDaab109014 was shown to react with GSN in wild-type HeLa cells in Western blot with loss of signal observed in GSN knockout cell line ab265201 (GSN knockout cell lysate ab257204). Wild-type HeLa and GSN knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 5% milk in TBST for 1 hr before incubation with ab109014 overnight at 4 °C at a 1/20000 dilution. Blots were incubated with goat anti-rabbit HRP secondary antibodies at 1/5000 before imaging. These data were provided by YCharOS Inc., an open science company with the mission of characterizing commercially available antibody reagents for all human proteins. Abcam and YCharOS are working together to help address the reproducibility crisis by enabling the life science community to better evaluate commercially available antibodies.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Gelsolin antibody [EPR1942] (ab109014)](https://www.abcam.com/ps/products/109/ab109014/Images/ab109014-121583-anti-gelsolin-antibody-epr1942-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Gelsolin antibody [EPR1942] (ab109014)Immunohistochemical analysis of Gelsolin expression in paraffin-embedded human kidney tissue using ab109014 at 1/250 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Western blot - Anti-Gelsolin antibody [EPR1942] (ab109014)](https://www.abcam.com/ps/products/109/ab109014/Images/ab109014-260342-anti-gelsolin-antibody-epr1942-western-blot.jpg)
Western blot - Anti-Gelsolin antibody [EPR1942] (ab109014)All lanes : Anti-Gelsolin antibody [EPR1942] (ab109014) at 1/20000 dilution
Lane 1 : THP-1 whole cell lysate
Lane 2 : THP-1 cultured medium
Lane 3 : MCF-7 whole cell lysate
Lane 4 : Human Plasma
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 86 kDa
Observed band size: 81,86 kDa why is the actual band size different from the predicted?
Exposure time: 10 secondsBlocking buffer 5% NFDM/TBST
Diluting buffer 5% NFDM/TBSTThe secretory isoform of gelsolin is at 86kDa. The cytoplasmic isoforms of gelsolin are at 81kDa.
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![Western blot - Anti-Gelsolin antibody [EPR1942] (ab109014)](https://www.abcam.com/ps/products/109/ab109014/Images/ab109014-121584-anti-gelsolin-antibody-epr1942-western-blot.jpg)
Western blot - Anti-Gelsolin antibody [EPR1942] (ab109014)All lanes : Anti-Gelsolin antibody [EPR1942] (ab109014) at 1/50000 dilution
Lane 1 : MCF7 cell lysate
Lane 2 : HL60 cell lysate
Lane 3 : A431 cell lysate
Lane 4 : HeLa cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 86 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Gelsolin antibody [EPR1942] (ab109014)](https://www.abcam.com/ps/products/109/ab109014/Images/ab109014-121582-anti-gelsolin-antibody-epr1942-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Gelsolin antibody [EPR1942] (ab109014)Immunohistochemical analysis of Gelsolin expression in paraffin-embedded Human lung tissue using ab109014 at 1/250 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Anti-Gelsolin antibody [EPR1942] (ab109014)](https://www.abcam.com/ps/products/109/ab109014/Images/ab109014-7-benefits-of-recombinant-antibodies.png)
Anti-Gelsolin antibody [EPR1942] (ab109014)
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (7)
ab109014 has been referenced in 7 publications.
- Bhopale VM et al. Plasma gelsolin modulates the production and fate of IL-1ß-containing microparticles following high-pressure exposure and decompression. J Appl Physiol (1985) 130:1604-1613 (2021). PubMed: 33764168
- Oggero S et al. Extracellular vesicles from monocyte/platelet aggregates modulate human atherosclerotic plaque reactivity. J Extracell Vesicles 10:12084 (2021). PubMed: 33936566
- Zhang J et al. Gelsolin inhibits malignant phenotype of glioblastoma and is regulated by miR-654-5p and miR-450b-5p. Cancer Sci 111:2413-2422 (2020). PubMed: 32324311
- Stock K et al. Overexpression and Tyr421-phosphorylation of cortactin is induced by three-dimensional spheroid culturing and contributes to migration and invasion of pancreatic ductal adenocarcinoma (PDAC) cells. Cancer Cell Int 19:77 (2019). PubMed: 30976201
- Lu H et al. iTRAQ plasma proteomics analysis for candidate biomarkers of type 2 incipient diabetic nephropathy. Clin Proteomics 16:33 (2019). PubMed: 31384238
- Minghui Z et al. Analysis of Differentially Expressed Proteins Involved in Autoimmune Cirrhosis and Normal Serum by iTRAQ Proteomics. Proteomics Clin Appl N/A:e1700153 (2018). PubMed: 29999587
- Mazur AJ et al. Analysis of gelsolin expression pattern in developing chicken embryo reveals high GSN expression level in tissues of neural crest origin. Brain Struct Funct N/A:N/A (2014). WB ; Chicken . PubMed: 25352156