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    products/primary-antibodies/gfap-antibody-2a5-ab4648.pdf

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Neuroscience Cell Adhesion Proteins Cytoskeletal Proteins Intermediate Filaments
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Anti-GFAP antibody [2A5] (ab4648)

  • Datasheet
  • SDS
Reviews (14)Q&A (3)References (51)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GFAP antibody [2A5] (ab4648)
  • Immunohistochemistry - Free Floating - Anti-GFAP antibody [2A5] (ab4648)
  • Western blot - Anti-GFAP antibody [2A5] (ab4648)
  • Immunocytochemistry/ Immunofluorescence - Anti-GFAP antibody [2A5] (ab4648)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GFAP antibody [2A5] (ab4648)
  • Immunocytochemistry/ Immunofluorescence - Anti-GFAP antibody [2A5] (ab4648)
  • Immunocytochemistry/ Immunofluorescence - Anti-GFAP antibody [2A5] (ab4648)
  • Immunocytochemistry/ Immunofluorescence - Anti-GFAP antibody [2A5] (ab4648)

Key features and details

  • Mouse monoclonal [2A5] to GFAP
  • Suitable for: IHC-FrFl, WB, IHC-P, ICC/IF
  • Reacts with: Mouse, Rat, Human, Pig
  • Isotype: IgG1

Get better batch-to-batch reproducibility with a recombinant antibody

Product image
Anti-GFAP antibody [EPR1034Y] (ab68428)
  • Research with confidence – consistent and reproducible results with every batch
  • Long-term and scalable supply – powered by recombinant technology for fast production
  • Success from the first experiment – confirmed specificity through extensive validation
  • Ethical standards compliant – production is animal-free

Overview

  • Product name

    Anti-GFAP antibody [2A5]
    See all GFAP primary antibodies
  • Description

    Mouse monoclonal [2A5] to GFAP
  • Host species

    Mouse
  • Tested applications

    Suitable for: IHC-FrFl, WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human, Pig
  • Immunogen

    Full length native protein (purified) corresponding to Pig GFAP. A preparation of purified pig spinal cord GFAP.

  • Positive control

    • IHC-P: Human brain tissue, human cerebellum tissue. IHC-FreeFloat: Rat cerebellum tissue: WB: Pig brain tissue lysate; Rat spinal cord tissue lysate; Mouse spinal cord tissue lysate. ICC: Rat neuron/glia cells.
  • General notes

    This clone gives a much stronger signal in pig, and human samples than in rodents. For rodent (mouse, rat ,et al.) samples use ab68428.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer

    Preservative: 0.065% Sodium azide
    Constituent: Tissue culture supernatant

    Concentrated Tissue Culture Supernatant
  • Concentration information loading...
  • Purity

    Tissue culture supernatant
  • Purification notes

    Antibody is supplied as Integra CL-350 flask material, which is concentrated tissue culture supernatant.
  • Clonality

    Monoclonal
  • Clone number

    2A5
  • Isotype

    IgG1
  • Light chain type

    kappa
  • Research areas

    • Neuroscience
    • Cell Adhesion Proteins
    • Cytoskeletal Proteins
    • Intermediate Filaments
    • Neuroscience
    • Cell Type Marker
    • Glia marker
    • Astrocyte marker
    • Signal Transduction
    • Cytoskeleton / ECM
    • Cytoskeleton
    • Intermediate Filaments
    • Class II
    • GFAP
    • Tags & Cell Markers
    • Cell Type Markers
    • Neuroscience Markers
    • Glial
    • Stem Cells
    • Neural Stem Cells
    • Glial Restricted Lineage
    • Astrocyte
    • Neuroscience
    • Development

Associated products

  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
  • Isotype control

    • Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)
  • Recombinant Protein

    • Recombinant Human GFAP protein (ab114149)
    • Recombinant Human GFAP protein (ab151370)
  • Related Products

    • Human GFAP Matched Antibody Pair Kit (ab222279)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab4648 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-FrFl
1/500.
WB (1)
1/2000. Predicted molecular weight: 49 kDa.
IHC-P (2)
1/500.
ICC/IF (5)
1/10 - 1/50.
Notes
IHC-FrFl
1/500.
WB
1/2000. Predicted molecular weight: 49 kDa.
IHC-P
1/500.
ICC/IF
1/10 - 1/50.

Target

  • Function

    GFAP, a class-III intermediate filament, is a cell-specific marker that, during the development of the central nervous system, distinguishes astrocytes from other glial cells.
  • Tissue specificity

    Expressed in cells lacking fibronectin.
  • Involvement in disease

    Defects in GFAP are a cause of Alexander disease (ALEXD) [MIM:203450]. Alexander disease is a rare disorder of the central nervous system. It is a progressive leukoencephalopathy whose hallmark is the widespread accumulation of Rosenthal fibers which are cytoplasmic inclusions in astrocytes. The most common form affects infants and young children, and is characterized by progressive failure of central myelination, usually leading to death usually within the first decade. Infants with Alexander disease develop a leukoencephalopathy with macrocephaly, seizures, and psychomotor retardation. Patients with juvenile or adult forms typically experience ataxia, bulbar signs and spasticity, and a more slowly progressive course.
  • Sequence similarities

    Belongs to the intermediate filament family.
  • Post-translational
    modifications

    Phosphorylated by PKN1.
  • Cellular localization

    Cytoplasm. Associated with intermediate filaments.
  • Target information above from: UniProt accession P14136 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 2670 Human
    • Entrez Gene: 14580 Mouse
    • Entrez Gene: 396562 Pig
    • Entrez Gene: 24387 Rat
    • Omim: 137780 Human
    • SwissProt: P14136 Human
    • SwissProt: P03995 Mouse
    • SwissProt: P47819 Rat
    • Unigene: 514227 Human
    • Unigene: 1239 Mouse
    • Unigene: 91512 Rat
    see all
  • Alternative names

    • wu:fb34h11 antibody
    • ALXDRD antibody
    • cb345 antibody
    • etID36982.3 antibody
    • FLJ42474 antibody
    • FLJ45472 antibody
    • GFAP antibody
    • GFAP_HUMAN antibody
    • gfapl antibody
    • Glial fibrillary acidic protein antibody
    • Intermediate filament protein antibody
    • wu:fk42c12 antibody
    • xx:af506734 antibody
    • zgc:110485 antibody
    see all

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GFAP antibody [2A5] (ab4648)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GFAP antibody [2A5] (ab4648)

    Immunohistochemistry analysis of paraffin-embedded human cerebellum tissue sections labelling GFAP with ab4648 at 1/500 dilution (1 mg/mL). Sections were stained with ab4648 using the HRP/DAB staining. Sections were counterstained with hematoxylin/eosin. Antigen retrieval was heat mediated using Antigen retrieval buffer (100X citrate buffer) (pH 6.0) (ab93678) for 15 minutes.
    To the left is a region of cerebellar molecular layer containing the prominent cytoskeletal fibers of Bergmann glia which are strongly positive for GFAP. The middle shows a region of the granular layer and to the right is white matter, both of which contain GFAP positive astrocytes.The immunostaining was performed with the Vector ImmPress rat adsorbed horse anti-mouse IgG detection kit.

  • Immunohistochemistry - Free Floating - Anti-GFAP antibody [2A5] (ab4648)
    Immunohistochemistry - Free Floating - Anti-GFAP antibody [2A5] (ab4648)

    Immunofluorescence analysis of rat cerebellum tissue labeling GFAP with ab4648 at 1/500 dilution (Red), Costained with parvalbumin antibody at 1/2,000 dilution (Green). The blue is DAPI staining of nuclear DNA. Following transcardial perfusion of rat with 4% paraformaldehyde, brain was post fixed for 24 hours, and free-floating 45μM sections were stained with above antibodies. ab4648 stains the processes of Bergmann glia and astrocytes. The Pvalb antibody labels perikarya and dendrites of Purkinje cells and interneurons in the molecular layer of the cerebellum. The staining on rodent tissues is specific but not as robust as on human material.

     

  • Western blot - Anti-GFAP antibody [2A5] (ab4648)
    Western blot - Anti-GFAP antibody [2A5] (ab4648)
    Lanes 2-8 : Anti-GFAP antibody [2A5] (ab4648) at 1/2000 dilution

    Lane 1 : MW markers
    Lane 2 : Rat brain tissue lysate
    Lane 3 : Rat spinal cord tissue lysate
    Lane 4 : Mouse brain tissue lysate
    Lane 5 : Mouse spinal cord tissue lysate
    Lane 6 : Pig brain tissue lysate
    Lane 7 : Recombinant rat GFAP protein
    Lane 8 : Recombinant human GFAP protein

    Predicted band size: 49 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-GFAP antibody [2A5] (ab4648)
    Immunocytochemistry/ Immunofluorescence - Anti-GFAP antibody [2A5] (ab4648)

    Rat neuron/glia cultures stained with mouse monoclonal to GFAP ab 4648 (red).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GFAP antibody [2A5] (ab4648)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GFAP antibody [2A5] (ab4648)
    Ab4648 staining human substantia nigra. Staining is localised to the cytoplasm.
    Left panel: with primary antibody diluted 1:4000. Right panel: isotype control.
    Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 AR buffer citrate pH 6.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.
  • Immunocytochemistry/ Immunofluorescence - Anti-GFAP antibody [2A5] (ab4648)
    Immunocytochemistry/ Immunofluorescence - Anti-GFAP antibody [2A5] (ab4648)
    Rat cortical neurons and glia in mixed tissue culture stained with Chicken polyclonal to MAP2 - ab5392 (green) at 1/30000 and Mouse monoclonal to GFAP - ab4648 (red) at 1/100. Nuclei of all cells are stained with Hoechst dye (blue). Picture taken with a Zeiss 20X objective and documented with a Digital SPOT camera.
  • Immunocytochemistry/ Immunofluorescence - Anti-GFAP antibody [2A5] (ab4648)
    Immunocytochemistry/ Immunofluorescence - Anti-GFAP antibody [2A5] (ab4648)
    Rat neuron/glia cultures stained with mouse monoclonal to GFAP ab4648 (red).
  • Immunocytochemistry/ Immunofluorescence - Anti-GFAP antibody [2A5] (ab4648)
    Immunocytochemistry/ Immunofluorescence - Anti-GFAP antibody [2A5] (ab4648)
    Rat neuron/glia cultures stained with mouse monoclonal to GFAP ab4648 (green).

Protocols

  • Recommended ICC protocol with ab4648
  • Protocol Booklet

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (51)

Publishing research using ab4648? Please let us know so that we can cite the reference in this datasheet.

ab4648 has been referenced in 51 publications.

  • Qin L  et al. TRAIL Mediates Neuronal Death in AUD: A Link between Neuroinflammation and Neurodegeneration. Int J Mol Sci 22:N/A (2021). PubMed: 33806288
  • Ahmad F  et al. Lentivirus vector-mediated genetic manipulation of oncogenic pathways induces tumor formation in rabbit brain. Mol Med Rep 23:N/A (2021). PubMed: 33846766
  • Lind L  et al. CD8+?T cells in the central nervous system of mice with herpes simplex infection are highly activated and express high levels of CCR5 and CXCR3. J Neurovirol 27:145-153 (2021). PubMed: 33492607
  • Liu X  et al. IL-9-triggered lncRNA Gm13568 regulates Notch1 in astrocytes through interaction with CBP/P300: contribute to the pathogenesis of experimental autoimmune encephalomyelitis. J Neuroinflammation 18:108 (2021). PubMed: 33971906
  • Vetreno RP  et al. Increased Toll-like Receptor-MyD88-NF?B-Proinflammatory neuroimmune signaling in the orbitofrontal cortex of humans with alcohol use disorder. Alcohol Clin Exp Res 45:1747-1761 (2021). PubMed: 34415075
View all Publications for this product

Customer reviews and Q&As

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1-5 of 5 Abreviews or Q&A

Immunocytochemistry/ Immunofluorescence abreview for Anti-GFAP antibody [2A5]

Good
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Rat Cell (Cortical)
Permeabilization
Yes - 1% TritonX-100
Specification
Cortical
Blocking step
BSA as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Fixative
Paraformaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Apr 15 2022

Immunocytochemistry/ Immunofluorescence abreview for Anti-GFAP antibody [2A5]

Good
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Neuron stem cell)
Permeabilization
Yes - 1% TritonX-100
Specification
Neuron stem cell
Blocking step
BSA as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 25°C
Fixative
Paraformaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Apr 15 2022

Immunocytochemistry/ Immunofluorescence abreview for Anti-GFAP antibody [2A5]

Excellent
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (mouse neuronal culture)
Permeabilization
No
Specification
mouse neuronal culture
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Fixative
Methanol
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Jan 17 2022

Immunocytochemistry/ Immunofluorescence abreview for Anti-GFAP antibody [2A5]

Poor
Abreviews
Abreviews
Application
Immunocytochemistry/ Immunofluorescence
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: RTP°C
Sample
Mouse Cell (Mouse primary astrocyte)
Specification
Mouse primary astrocyte
Permeabilization
Yes - 0.1% Triton X-100
Fixative
Paraformaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Dr. Craig Beall

Verified customer

Submitted Sep 15 2014

Immunocytochemistry/ Immunofluorescence abreview for Anti-GFAP antibody [2A5] - Astrocyte Marker

Excellent
Abreviews
Abreviews
Application
Immunocytochemistry/ Immunofluorescence
Sample
Rat Cell (Embryonic rat cerebral cortex)
Specification
Embryonic rat cerebral cortex
Fixative
Paraformaldehyde
Permeabilization
Yes - Ice cold methanol
Blocking step
Serum as blocking agent for 15 minute(s) · Concentration: 5% · Temperature: 25°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Dec 26 2012

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