Anti-GFAP antibody [2A5] (ab4648)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GFAP antibody [2A5] (ab4648)](https://www.abcam.com/ps/products/4/ab4648/Images/ab4648-487864-anti-gfap-antibody-2A5-immunohistochemistry-human.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GFAP antibody [2A5] (ab4648)")
Key features and details
- Mouse monoclonal [2A5] to GFAP
- Suitable for: IHC-FrFl, WB, IHC-P, ICC/IF
- Reacts with: Mouse, Rat, Human, Pig
- Isotype: IgG1
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
- Long-term and scalable supply – powered by recombinant technology for fast production
- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
-
Product name
Anti-GFAP antibody [2A5]
See all GFAP primary antibodies -
Description
Mouse monoclonal [2A5] to GFAP -
Host species
Mouse -
Tested applications
Suitable for: IHC-FrFl, WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Rat, Human, Pig -
Immunogen
Full length native protein (purified) corresponding to Pig GFAP. A preparation of purified pig spinal cord GFAP.
-
Positive control
- IHC-P: Human brain tissue, human cerebellum tissue. IHC-FreeFloat: Rat cerebellum tissue: WB: Pig brain tissue lysate; Rat spinal cord tissue lysate; Mouse spinal cord tissue lysate. ICC: Rat neuron/glia cells.
-
General notes
This clone gives a much stronger signal in pig, and human samples than in rodents. For rodent (mouse, rat ,et al.) samples use ab68428.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
Preservative: 0.065% Sodium azide
Constituent: Tissue culture supernatant
Concentrated Tissue Culture Supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Purification notes
Antibody is supplied as Integra CL-350 flask material, which is concentrated tissue culture supernatant. -
Clonality
Monoclonal -
Clone number
2A5 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
-
Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab4648 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-FrFl |
1/500.
|
|
| WB | (1) |
1/2000. Predicted molecular weight: 49 kDa.
|
| IHC-P | (2) |
1/500.
|
| ICC/IF | (5) |
1/10 - 1/50.
|
| Notes |
|---|
|
IHC-FrFl
1/500. |
|
WB
1/2000. Predicted molecular weight: 49 kDa. |
|
IHC-P
1/500. |
|
ICC/IF
1/10 - 1/50. |
Target
-
Function
GFAP, a class-III intermediate filament, is a cell-specific marker that, during the development of the central nervous system, distinguishes astrocytes from other glial cells. -
Tissue specificity
Expressed in cells lacking fibronectin. -
Involvement in disease
Defects in GFAP are a cause of Alexander disease (ALEXD) [MIM:203450]. Alexander disease is a rare disorder of the central nervous system. It is a progressive leukoencephalopathy whose hallmark is the widespread accumulation of Rosenthal fibers which are cytoplasmic inclusions in astrocytes. The most common form affects infants and young children, and is characterized by progressive failure of central myelination, usually leading to death usually within the first decade. Infants with Alexander disease develop a leukoencephalopathy with macrocephaly, seizures, and psychomotor retardation. Patients with juvenile or adult forms typically experience ataxia, bulbar signs and spasticity, and a more slowly progressive course. -
Sequence similarities
Belongs to the intermediate filament family. -
Post-translational
modificationsPhosphorylated by PKN1. -
Cellular localization
Cytoplasm. Associated with intermediate filaments. - Information by UniProt
-
Database links
- Entrez Gene: 2670 Human
- Entrez Gene: 14580 Mouse
- Entrez Gene: 396562 Pig
- Entrez Gene: 24387 Rat
- Omim: 137780 Human
- SwissProt: P14136 Human
- SwissProt: P03995 Mouse
- SwissProt: P47819 Rat
see all -
Alternative names
- wu:fb34h11 antibody
- ALXDRD antibody
- cb345 antibody
see all
Images
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GFAP antibody [2A5] (ab4648)
Immunohistochemistry analysis of paraffin-embedded human cerebellum tissue sections labelling GFAP with ab4648 at 1/500 dilution (1 mg/mL). Sections were stained with ab4648 using the HRP/DAB staining. Sections were counterstained with hematoxylin/eosin. Antigen retrieval was heat mediated using Antigen retrieval buffer (100X citrate buffer) (pH 6.0) (ab93678) for 15 minutes.
To the left is a region of cerebellar molecular layer containing the prominent cytoskeletal fibers of Bergmann glia which are strongly positive for GFAP. The middle shows a region of the granular layer and to the right is white matter, both of which contain GFAP positive astrocytes.The immunostaining was performed with the Vector ImmPress rat adsorbed horse anti-mouse IgG detection kit. -
![Immunohistochemistry - Free Floating - Anti-GFAP antibody [2A5] (ab4648)](https://www.abcam.com/ps/products/4/ab4648/Images/ab4648-402154-anti-gfap-antibody-if-rat-cerebellum-tissue.JPG)
Immunohistochemistry - Free Floating - Anti-GFAP antibody [2A5] (ab4648)Immunofluorescence analysis of rat cerebellum tissue labeling GFAP with ab4648 at 1/500 dilution (Red), Costained with parvalbumin antibody at 1/2,000 dilution (Green). The blue is DAPI staining of nuclear DNA. Following transcardial perfusion of rat with 4% paraformaldehyde, brain was post fixed for 24 hours, and free-floating 45μM sections were stained with above antibodies. ab4648 stains the processes of Bergmann glia and astrocytes. The Pvalb antibody labels perikarya and dendrites of Purkinje cells and interneurons in the molecular layer of the cerebellum. The staining on rodent tissues is specific but not as robust as on human material.
-
![Western blot - Anti-GFAP antibody [2A5] (ab4648)](https://www.abcam.com/ps/products/4/ab4648/Images/ab4648-402153-anti-gfap-antibody-wb-rat-and-mouse-samples.JPG)
Western blot - Anti-GFAP antibody [2A5] (ab4648)Lanes 2-8 : Anti-GFAP antibody [2A5] (ab4648) at 1/2000 dilution
Lane 1 : MW markers
Lane 2 : Rat brain tissue lysate
Lane 3 : Rat spinal cord tissue lysate
Lane 4 : Mouse brain tissue lysate
Lane 5 : Mouse spinal cord tissue lysate
Lane 6 : Pig brain tissue lysate
Lane 7 : Recombinant rat GFAP protein
Lane 8 : Recombinant human GFAP protein
Predicted band size: 49 kDa -
![Immunocytochemistry/ Immunofluorescence - Anti-GFAP antibody [2A5] (ab4648)](https://www.abcam.com/ps/products/4/ab4648/Images/ab4648-9395-anti-gfap-antibody-2a5-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-GFAP antibody [2A5] (ab4648)Rat neuron/glia cultures stained with mouse monoclonal to GFAP ab 4648 (red).
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GFAP antibody [2A5] (ab4648)](https://www.abcam.com/ps/products/4/ab4648/Images/ab4648-9393-anti-gfap-antibody-2a5-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GFAP antibody [2A5] (ab4648)Ab4648 staining human substantia nigra. Staining is localised to the cytoplasm.
Left panel: with primary antibody diluted 1:4000. Right panel: isotype control.
Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 AR buffer citrate pH 6.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required. -
![Immunocytochemistry/ Immunofluorescence - Anti-GFAP antibody [2A5] (ab4648)](https://www.abcam.com/ps/products/4/ab4648/Images/ab4648-9397-anti-gfap-antibody-2a5-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-GFAP antibody [2A5] (ab4648)Rat cortical neurons and glia in mixed tissue culture stained with Chicken polyclonal to MAP2 - ab5392 (green) at 1/30000 and Mouse monoclonal to GFAP - ab4648 (red) at 1/100. Nuclei of all cells are stained with Hoechst dye (blue). Picture taken with a Zeiss 20X objective and documented with a Digital SPOT camera. -
![Immunocytochemistry/ Immunofluorescence - Anti-GFAP antibody [2A5] (ab4648)](https://www.abcam.com/ps/products/4/ab4648/Images/ab4648-9396-anti-gfap-antibody-2a5-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-GFAP antibody [2A5] (ab4648)Rat neuron/glia cultures stained with mouse monoclonal to GFAP ab4648 (red). -
![Immunocytochemistry/ Immunofluorescence - Anti-GFAP antibody [2A5] (ab4648)](https://www.abcam.com/ps/products/4/ab4648/Images/ab4648-9394-anti-gfap-antibody-2a5-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-GFAP antibody [2A5] (ab4648)Rat neuron/glia cultures stained with mouse monoclonal to GFAP ab4648 (green).
Protocols
Datasheets and documents
-
SDS download
-
Datasheet download
References (69)
ab4648 has been referenced in 69 publications.
- Li W et al. 4,5-Dimethoxycanthin-6-one is a novel LSD1 inhibitor that inhibits proliferation of glioblastoma cells and induces apoptosis and pyroptosis. Cancer Cell Int 22:32 (2022). PubMed: 35042538
- Milky B et al. Long-term adherence of human brain cells in vitro is enhanced by charged amine-based plasma polymer coatings. Stem Cell Reports 17:489-506 (2022). PubMed: 35180396
- Xu T et al. Multiple therapeutic effects of human neural stem cells derived from induced pluripotent stem cells in a rat model of post-traumatic syringomyelia. EBioMedicine 77:103882 (2022). PubMed: 35182996
- Anastasaki C et al. Generation of human induced pluripotent stem cell-derived cerebral organoids for cellular and molecular characterization. STAR Protoc 3:101173 (2022). PubMed: 35199037
- Xie LL et al. Melatonin alleviates traumatic brain injury-induced anxiety-like behaviors in rats: Roles of the protein kinase A/cAMP-response element binding signaling pathway. Exp Ther Med 23:248 (2022). PubMed: 35261620