Anti-GFAP antibody (ab53554)
Key features and details
- Goat polyclonal to GFAP
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
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- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-GFAP antibody
See all GFAP primary antibodies -
Description
Goat polyclonal to GFAP -
Host species
Goat -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human
Predicted to work with: Dog
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Immunogen
Synthetic peptide corresponding to Human GFAP aa 400 to the C-terminus (C terminal) (Cysteine residue).
Database link: P14136-1 -
Positive control
- WB: Human cerebellum and cerebral cortex lysates. Mouse and rat brain lysates.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, 0.5% Tris -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab53554 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | (3) |
Use a concentration of 0.001 - 0.1 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 50 kDa).
A 1 hour primary incubation is recommended for this product. We recommend blocking in milk (3% milk in TBST) for 1 hour at room temperature. |
| Notes |
|---|
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WB
Use a concentration of 0.001 - 0.1 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 50 kDa). A 1 hour primary incubation is recommended for this product. We recommend blocking in milk (3% milk in TBST) for 1 hour at room temperature. |
Target
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Function
GFAP, a class-III intermediate filament, is a cell-specific marker that, during the development of the central nervous system, distinguishes astrocytes from other glial cells. -
Tissue specificity
Expressed in cells lacking fibronectin. -
Involvement in disease
Defects in GFAP are a cause of Alexander disease (ALEXD) [MIM:203450]. Alexander disease is a rare disorder of the central nervous system. It is a progressive leukoencephalopathy whose hallmark is the widespread accumulation of Rosenthal fibers which are cytoplasmic inclusions in astrocytes. The most common form affects infants and young children, and is characterized by progressive failure of central myelination, usually leading to death usually within the first decade. Infants with Alexander disease develop a leukoencephalopathy with macrocephaly, seizures, and psychomotor retardation. Patients with juvenile or adult forms typically experience ataxia, bulbar signs and spasticity, and a more slowly progressive course. -
Sequence similarities
Belongs to the intermediate filament family. -
Post-translational
modificationsPhosphorylated by PKN1. -
Cellular localization
Cytoplasm. Associated with intermediate filaments. - Information by UniProt
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Database links
- Entrez Gene: 480495 Dog
- Entrez Gene: 2670 Human
- Entrez Gene: 14580 Mouse
- Entrez Gene: 24387 Rat
- Omim: 137780 Human
- SwissProt: P14136 Human
- SwissProt: P03995 Mouse
- SwissProt: P47819 Rat
see all -
Alternative names
- wu:fb34h11 antibody
- ALXDRD antibody
- cb345 antibody
see all
Images
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Western blot - Anti-GFAP antibody (ab53554)All lanes : Anti-GFAP antibody (ab53554) at 0.001 µg/ml
Lane 1 : Human cerebellum tissue lysate (in RIPA buffer)
Lane 2 : Human cerebral cortex tissue lysate (in RIPA buffer)
Lysates/proteins at 35 µg per lane.
Predicted band size: 50 kDaPrimary incubation 1 hour at room temperature. Detected by chemiluminescence.
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Western blot - Anti-GFAP antibody (ab53554)Lane 1 : Anti-GFAP antibody (ab53554) at 0.003 µg/ml
Lane 2 : Anti-GFAP antibody (ab53554) at 0.01 µg/ml
Lane 1 : Mouse brain tissue lysate (in RIPA buffer)
Lane 2 : Rat brain tissue lysate (in RIPA buffer)
Lysates/proteins at 35 µg per lane.
Predicted band size: 50 kDaPrimary incubation 1 hour at room temperature. Detected by chemiluminescence.
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Western blot - Anti-GFAP antibody (ab53554)This image was courtesy of an annonymous AbreviewAll lanes : Anti-GFAP antibody (ab53554) at 1/1000 dilution
All lanes : Human Tissue lysate - other (Frontal Cortex)
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : Donkey anti-goat IRDye 800CW conjugated Li-Cor IRdye
Predicted band size: 50 kDa
Observed band size: 40-50 kDa why is the actual band size different from the predicted?
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (320)
ab53554 has been referenced in 320 publications.
- Su Y et al. Astrocyte endfoot formation controls the termination of oligodendrocyte precursor cell perivascular migration during development. Neuron 111:190-201.e8 (2023). PubMed: 36384142
- Ding F et al. Astrocytes exhibit diverse Ca2+ changes at subcellular domains during brain aging. Front Aging Neurosci 14:1029533 (2022). PubMed: 36389078
- Nsiah NY & Inman DM Destabilizing COXIV in Müller Glia Increases Retinal Glycolysis and Alters Scotopic Electroretinogram. Cells 11:N/A (2022). PubMed: 36497016
- Pan AL et al. Dual-Specificity Protein Phosphatase 4 (DUSP4) Overexpression Improves Learning Behavior Selectively in Female 5xFAD Mice, and Reduces β-Amyloid Load in Males and Females. Cells 11:N/A (2022). PubMed: 36497141
- Kong C et al. Enhanced delivery of a low dose of aducanumab via FUS in 5×FAD mice, an AD model. Transl Neurodegener 11:57 (2022). PubMed: 36575534