Recombinant Anti-GFAP antibody [ASTRO/1974R] (ab270270)
![Flow Cytometry - Anti-GFAP antibody [ASTRO/1974R] (ab270270)](https://www.abcam.com/ps/products/270/ab270270/Images/ab270270-381153-anti-gfap-antibody-astro1974r-flow-t98g-cells.JPG "Flow Cytometry - Anti-GFAP antibody [ASTRO/1974R] (ab270270)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [ASTRO/1974R] to GFAP
- Suitable for: Flow Cyt, IHC-P
- Reacts with: Human
Related conjugates and formulations
Overview
-
Product name
Anti-GFAP antibody [ASTRO/1974R]
See all GFAP primary antibodies -
Description
Rabbit monoclonal [ASTRO/1974R] to GFAP -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Rabbit, Chicken, Cow, Pig
-
Immunogen
Recombinant full length protein corresponding to Human GFAP.
Database link: P14136 -
Positive control
- IHC-P: Human cerebellum tissue. Flow cyt: T98G cells.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.05% Sodium azide
Constituents: PBS, 0.05% BSA -
Concentration information loading... -
Purity
Protein A/G purified -
Purification notes
Purified from Bioreactor Concentrate by Protein A/G. -
Clonality
Monoclonal -
Clone number
ASTRO/1974R -
Isotype
IgG -
Research areas
Associated products
-
Alternative Versions
-
Compatible Secondaries
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab270270 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt |
Use 1-2µl for 106 cells.
|
|
| IHC-P |
Use a concentration of 1 - 2 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
|
| Notes |
|---|
|
Flow Cyt
Use 1-2µl for 106 cells. |
|
IHC-P
Use a concentration of 1 - 2 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Target
-
Function
GFAP, a class-III intermediate filament, is a cell-specific marker that, during the development of the central nervous system, distinguishes astrocytes from other glial cells. -
Tissue specificity
Expressed in cells lacking fibronectin. -
Involvement in disease
Defects in GFAP are a cause of Alexander disease (ALEXD) [MIM:203450]. Alexander disease is a rare disorder of the central nervous system. It is a progressive leukoencephalopathy whose hallmark is the widespread accumulation of Rosenthal fibers which are cytoplasmic inclusions in astrocytes. The most common form affects infants and young children, and is characterized by progressive failure of central myelination, usually leading to death usually within the first decade. Infants with Alexander disease develop a leukoencephalopathy with macrocephaly, seizures, and psychomotor retardation. Patients with juvenile or adult forms typically experience ataxia, bulbar signs and spasticity, and a more slowly progressive course. -
Sequence similarities
Belongs to the intermediate filament family. -
Post-translational
modificationsPhosphorylated by PKN1. -
Cellular localization
Cytoplasm. Associated with intermediate filaments. - Information by UniProt
-
Database links
- Entrez Gene: 281189 Cow
- Entrez Gene: 2670 Human
- Entrez Gene: 14580 Mouse
- Entrez Gene: 396562 Pig
- Entrez Gene: 100352532 Rabbit
- Entrez Gene: 24387 Rat
- Omim: 137780 Human
- SwissProt: Q28115 Cow
see all -
Alternative names
- wu:fb34h11 antibody
- ALXDRD antibody
- cb345 antibody
see all
Images
-
Flow Cytometry - Anti-GFAP antibody [ASTRO/1974R] (ab270270)
Flow cytometric analysis of T98G cells labeling GFAP (Blue) using ab270270 at 2 μg/106 cells, followed by Goat anti-Rabbit IgG-CF488 (Blue); Isotype Control (Red).
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GFAP antibody [ASTRO/1974R] (ab270270)](https://www.abcam.com/ps/products/270/ab270270/Images/ab270270-381150-anti-gfap-antibody-astro1974r-ihc-human-cerebellum.JPG)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GFAP antibody [ASTRO/1974R] (ab270270)Formalin-fixed, paraffin-embedded human cerebellum tissue stained for GFAP using ab270270 at 2 μg/ml in imunnohistochemical analysis.
-
![Anti-GFAP antibody [ASTRO/1974R] (ab270270)](https://www.abcam.com/ps/products/270/ab270270/Images/ab270270-3-benefits-of-recombinant-antibodies.png)
Anti-GFAP antibody [ASTRO/1974R] (ab270270)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
-
Datasheet download
References (0)
ab270270 has not yet been referenced specifically in any publications.
