Anti-GFAP antibody [ZRF-1] (ab154474)
![Western blot - Anti-GFAP antibody [ZRF-1] (ab154474)](https://www.abcam.com/ps/products/154/ab154474/Images/ab154474-160654-anti-gfap-antibody-zrf-1-western-blot.jpg "Western blot - Anti-GFAP antibody [ZRF-1] (ab154474)")
Key features and details
- Mouse monoclonal [ZRF-1] to GFAP
- Suitable for: WB, IP
- Reacts with: Human, Zebrafish
- Isotype: IgG1
Overview
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Product name
Anti-GFAP antibody [ZRF-1]
See all GFAP primary antibodies -
Description
Mouse monoclonal [ZRF-1] to GFAP -
Host species
Mouse -
Tested applications
Suitable for: WB, IPmore details -
Species reactivity
Reacts with: Human, Zebrafish
Does not react with: Mouse, Rat -
Immunogen
Tissue, cells or virus. This information is considered to be commercially sensitive.
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Positive control
- Human brain and Zebrafish brain homogenates.
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General notes
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Product was previously marketed under the MitoSciences sub-brand.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.5
Preservative: 0.02% Sodium azide
Constituent: 99% HEPES buffered saline -
Concentration information loading... -
Purification notes
Near homogeneity as judged by SDS-PAGE. ab154474 was produced in vitro using hybridomas grown in serum-free medium, and then concentrated by chemical fractionation. -
Clonality
Monoclonal -
Clone number
ZRF-1 -
Isotype
IgG1 -
Light chain type
lambda -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab154474 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
Use a concentration of 0.5 µg/ml. Predicted molecular weight: 51 kDa.
|
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| IP |
Use at an assay dependent concentration.
|
| Notes |
|---|
|
WB
Use a concentration of 0.5 µg/ml. Predicted molecular weight: 51 kDa. |
|
IP
Use at an assay dependent concentration. |
Target
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Function
GFAP, a class-III intermediate filament, is a cell-specific marker that, during the development of the central nervous system, distinguishes astrocytes from other glial cells. -
Tissue specificity
Expressed in cells lacking fibronectin. -
Involvement in disease
Defects in GFAP are a cause of Alexander disease (ALEXD) [MIM:203450]. Alexander disease is a rare disorder of the central nervous system. It is a progressive leukoencephalopathy whose hallmark is the widespread accumulation of Rosenthal fibers which are cytoplasmic inclusions in astrocytes. The most common form affects infants and young children, and is characterized by progressive failure of central myelination, usually leading to death usually within the first decade. Infants with Alexander disease develop a leukoencephalopathy with macrocephaly, seizures, and psychomotor retardation. Patients with juvenile or adult forms typically experience ataxia, bulbar signs and spasticity, and a more slowly progressive course. -
Sequence similarities
Belongs to the intermediate filament family. -
Post-translational
modificationsPhosphorylated by PKN1. -
Cellular localization
Cytoplasm. Associated with intermediate filaments. - Information by UniProt
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Database links
- Entrez Gene: 2670 Human
- Entrez Gene: 30646 Zebrafish
- Omim: 137780 Human
- SwissProt: P14136 Human
- SwissProt: Q58EE9 Zebrafish
- Unigene: 514227 Human
- Unigene: 32667 Zebrafish
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Alternative names
- wu:fb34h11 antibody
- ALXDRD antibody
- cb345 antibody
see all
Images
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Western blot - Anti-GFAP antibody [ZRF-1] (ab154474)All lanes : Anti-GFAP antibody [ZRF-1] (ab154474) at 0.5 µg/ml
Lane 1 : Human Brain Homogenate lysate
Lane 2 : Zebrafish Brain Homogenate lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Mouse IgG - AP at 1/3000 dilution
Predicted band size: 51 kDa -
![Immunoprecipitation - Anti-GFAP antibody [ZRF-1] (ab154474)](https://www.abcam.com/ps/products/154/ab154474/Images/ab154474-160653-anti-gfap-antibody-zrf-1-immunoprecipitation.jpg)
Immunoprecipitation - Anti-GFAP antibody [ZRF-1] (ab154474)Immunoprecipitation Western Blot for ab154474.
All lanes: ab154474 at 0.5 µg/ml.
Lane 1: Zebrafish Brain Homogenate lysate 10 µg
Lane 2: GFAP immunoprecipitated from 1 mg Zebrafish Brain Homogenate lysate 25µl.
Secondary: Goat polyclonal to Mouse IgG - AP at 1/3000 dilution.
Protocols
Datasheets and documents
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Datasheet download
References (5)
ab154474 has been referenced in 5 publications.
- Ceriani R & Whitlock KE Gonadotropin Releasing Hormone (Gnrh) Triggers Neurogenesis in the Hypothalamus of Adult Zebrafish. Int J Mol Sci 22:N/A (2021). PubMed: 34072957
- Demirci Y et al. Comparative Transcriptome Analysis of the Regenerating Zebrafish Telencephalon Unravels a Resource With Key Pathways During Two Early Stages and Activation of Wnt/ß-Catenin Signaling at the Early Wound Healing Stage. Front Cell Dev Biol 8:584604 (2020). PubMed: 33163496
- McCallum S et al. Enteric glia as a source of neural progenitors in adult zebrafish. Elife 9:N/A (2020). PubMed: 32851974
- Luo JJ et al. CRISPR/Cas9-based genome engineering of zebrafish using a seamless integration strategy. FASEB J 32:5132-5142 (2018). PubMed: 29812974
- Yang Q et al. Serotonin Activated Hepatic Stellate Cells Contribute to Sex Disparity in Hepatocellular Carcinoma. Cell Mol Gastroenterol Hepatol 3:484-499 (2017). IF ; Zebrafish . PubMed: 28462385
