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    products/primary-antibodies/gfp-antibody-ab290.pdf

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Tags & Cell Markers Fusion / Marker Proteins GFP
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Anti-GFP antibody (ab290)

  • Datasheet
  • SDS
Reviews (178)Q&A (90)References (2676)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GFP antibody (ab290)
  • Immunohistochemistry - Free Floating - Anti-GFP antibody (ab290)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GFP antibody (ab290)
  • Immunocytochemistry - Anti-GFP antibody (ab290)
  • Immunoprecipitation - Anti-GFP antibody (ab290)
  • Immunocytochemistry - Anti-GFP antibody (ab290)
  • Immunoprecipitation - Anti-GFP antibody (ab290)
  • Immunocytochemistry - Anti-GFP antibody (ab290)
  • Western blot - Anti-GFP antibody (ab290)
  • Western blot - Anti-GFP antibody (ab290)
  • Western blot - Anti-GFP antibody (ab290)

Key features and details

  • Rabbit polyclonal to GFP
  • Suitable for: ELISA, IHC-Fr, ICC, IHC-P, IP, WB, IHC-FoFr, IHC-FrFl, Electron Microscopy
  • Reacts with: Species independent
  • Isotype: IgG

Get better batch-to-batch reproducibility with a recombinant antibody

Product image
Anti-GFP antibody [EPR14104] (ab183734)
  • Research with confidence – consistent and reproducible results with every batch
  • Long-term and scalable supply – powered by recombinant technology for fast production
  • Success from the first experiment – confirmed specificity through extensive validation
  • Ethical standards compliant – production is animal-free

Overview

  • Product name

    Anti-GFP antibody
    See all GFP primary antibodies
  • Description

    Rabbit polyclonal to GFP
  • Host species

    Rabbit
  • Specificity

    Anti-GFP antibody (ab290) is a highly versatile antibody that gives a stronger signal than other anti-GFP antibodies available. On Western blot the antibody detects the GFP fraction from cell extracts expressing recombinant GFP fusion proteins and has also been shown to be useful on mouse sections fixed with formalin. In Immunocytochemistry, the antibody gives a very good signal on recombinant YES-GFP chimeras expressed in COS cells (McCabe et al. 1999 and figure below). It is routinely used in Immunoprecipitation (IP) and IP-Western protocols and has been used successfully in HRP Immunohistochemistry at 1:200 on whole-mount mouse embryos.

    GFP antibody is reactive against all variants of Aequorea victoria GFP such as S65T-GFP, RS-GFP, YFP, CFP, RFP and EGFP.

  • Tested applications

    Suitable for: ELISA, IHC-Fr, ICC, IHC-P, IP, WB, IHC-FoFr, IHC-FrFl, Electron Microscopymore details
  • Species reactivity

    Reacts with: Species independent
  • Immunogen

    Recombinant full length protein corresponding to GFP. Green fluorescent protein (GFP) from Aequorea victoria.
    Database link: P42212

  • Positive control

    • The Recombinant A. victoria GFP protein (ab84191), any other purified recombinant GFP, any cell line confirmed to overexpress GFP. ICC: NIH3T3, U2OS and glandular stomach cells. IHC: Mouse brain and dog heart tissue. WB: Sample: COS7 and LNCaP whole cell lysate - transfected with GFP-Eml4.
  • General notes

    The total IgG concentration has been determined to be 5 mg/mL. The specific IgG concentration is unknown. This product should be kept refrigerated at all times whilst in short term storage. Using sterilised equipment will reduce the risk of bacterial contamination. 

    Anti-GFP antibody (ab6556) is the purified version of this antibody (see Related Products).

     

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.05% Sodium azide
    Constituent: 1.25% Sodium chloride
  • Concentration information loading...
  • Purity

    Whole antiserum
  • Purification notes

    This antibody is provided as whole antiserum. It is not possible to determine the exact antibody concentration, since whole serum contains many other host serum proteins besides the antibody of interest.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Tags & Cell Markers
    • Fusion / Marker Proteins
    • GFP

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 555) (ab150078)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Goat Anti-Rabbit IgG H&L (FITC) (ab6717)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Recombinant Protein

    • Recombinant A. victoria GFP protein (ab84191)
  • Related Products

    • GFP ELISA Kit (ab171581)
    • Anti-GFP antibody (ab6556)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab290 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA
Use at an assay dependent concentration.
IHC-Fr (8)
Use at an assay dependent concentration.

Reported to work at dilutions up to 1/3000. Use secondary antibody Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab15077). 

ICC (2)
1/200 - 1/1000.

We recommend Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150081) secondary antibody.

IHC-P (24)
1/500 - 1/1000. Perform heat mediated antigen retrieval via the microwave method before commencing with IHC staining protocol.
IP (24)
Use at an assay dependent concentration. Use at 1µl per 10cm tissue culture dish (use 10µl protein A agarose CL4B to precipitate the immune complex).
WB (69)
1/1000 - 1/2500.

It is recommended to use 12.5% SDS-PAGE and to transfer to PVDF membrane. Use 1x Blotto (or 3% BSA in PBS) for diluting and blocking. Use PBS in 3x 5min washing steps throughout the immunolabelling. Probe with ab290 at 1:1000 - 1:5000 dilution and use Goat Anti-Rabbit IgG H&L (HRP) (ab205718) at 1:5000 dilution with ECL detection method. ab290 has been reported to work at 1:50,000 and dilutions around this range should be tested if high background is seen. Both incubation steps should be for 1hr at 22°C.

IHC-FoFr (5)
1/200 - 1/500.
IHC-FrFl (2)
Use at an assay dependent concentration.
Electron Microscopy
1/1000 - 1/4000.
Notes
ELISA
Use at an assay dependent concentration.
IHC-Fr
Use at an assay dependent concentration.

Reported to work at dilutions up to 1/3000. Use secondary antibody Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab15077). 

ICC
1/200 - 1/1000.

We recommend Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150081) secondary antibody.

IHC-P
1/500 - 1/1000. Perform heat mediated antigen retrieval via the microwave method before commencing with IHC staining protocol.
IP
Use at an assay dependent concentration. Use at 1µl per 10cm tissue culture dish (use 10µl protein A agarose CL4B to precipitate the immune complex).
WB
1/1000 - 1/2500.

It is recommended to use 12.5% SDS-PAGE and to transfer to PVDF membrane. Use 1x Blotto (or 3% BSA in PBS) for diluting and blocking. Use PBS in 3x 5min washing steps throughout the immunolabelling. Probe with ab290 at 1:1000 - 1:5000 dilution and use Goat Anti-Rabbit IgG H&L (HRP) (ab205718) at 1:5000 dilution with ECL detection method. ab290 has been reported to work at 1:50,000 and dilutions around this range should be tested if high background is seen. Both incubation steps should be for 1hr at 22°C.

IHC-FoFr
1/200 - 1/500.
IHC-FrFl
Use at an assay dependent concentration.
Electron Microscopy
1/1000 - 1/4000.

Target

  • Relevance

    Function: Energy-transfer acceptor. Its role is to transduce the blue chemiluminescence of the protein aequorin into green fluorescent light by energy transfer. Fluoresces in vivo upon receiving energy from the Ca2+ -activated photoprotein aequorin.

    Subunit structure: Monomer.

    Tissue specificity: Photocytes.

    Post-translational modification: Contains a chromophore consisting of modified amino acid residues. The chromophore is formed by autocatalytic backbone condensation between Ser-65 and Gly-67, and oxidation of Tyr-66 to didehydrotyrosine. Maturation of the chromophore requires nothing other than molecular oxygen.

    Biotechnological use: Green fluorescent protein has been engineered to produce a vast number of variously colored mutants, fusion proteins, and biosensors. Fluorescent proteins and its mutated allelic forms, blue, cyan and yellow have become a useful and ubiquitous tool for making chimeric proteins, where they function as a fluorescent protein tag. Typically they tolerate N- and C-terminal fusion to a broad variety of proteins. They have been expressed in most known cell types and are used as a noninvasive fluorescent marker in living cells and organisms. They enable a wide range of applications where they have functioned as a cell lineage tracer, reporter of gene expression, or as a measure of protein-protein interactions. Can also be used as a molecular thermometer, allowing accurate temperature measurements in fluids. The measurement process relies on the detection of the blinking of GFP using fluorescence correlation spectroscopy.

    Sequence similarities: Belongs to the GFP family.

    Biophysicochemical properties: Absorption: Abs(max)=395 nm
    Exhibits a smaller absorbance peak at 470 nm. The fluorescence emission spectrum peaks at 509 nm with a shoulder at 540 nm.
  • Alternative names

    • GFP antibody
    • Green fluorescent protein antibody

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GFP antibody (ab290)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GFP antibody (ab290)Image from Yang C et al., PLoS One. 2013;8(11):e79615. Fig 2.; doi:10.1371/journal.pone.0079615. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/

    Bone marrow-derived infiltrating cells in the stromal tissue of gastric intraepithelial tumor traced by GFP direct fluorescence.

    (A) Normal tissues of the glandular stomach of a regular GFP(−) control mouse. (B) Normal tissues of the glandular stomach of a GFP(+) transgenic control mouse; (C, E, D, F) An induced gastric intraepithelial neoplasia (GIN) in a bone marrow transplanted mouse. GFP(+) BMDCs tracked with direct fluorescence localized in the GIN stromal tissue are shown in C and E. The same GIN lesion slide stained by H&E after the fluorescence observation are shown in D and F. DAPI (A–C and E) and hematoxylin (D and F) are used to visualize nuclei, respectively. Locations of the images C and D in the images E and F, and the image E in the image F are marked in the corresponding color. The gastric glands and stromal cells are also labeled.

  • Immunohistochemistry - Free Floating - Anti-GFP antibody (ab290)
    Immunohistochemistry - Free Floating - Anti-GFP antibody (ab290)This image is courtesy of an Abreview submitted by Judith Kranz

    Immunohistochemistry (Free Floating) analysis of mouse brain tissue sections labelling GFP with ab290. Tissue was fixed with 4% PFA, frozen 30 µm sections were blocked for 1 hour at room temperature with 10% normal goat serum + donkey anti-mouse IgG Fab fragments (0.1 mg/ml). Sections were incubated with the primary antibody at a dilution of 1/1000 in TBS + 0.25% Triton-X for 16 hours at 4°C. A Cy2®-conjugated donkey anti-rabbit IgG (H+L) at a dilution of 1/200 was used as the secondary antibody.

    Image shows anti-NeuN (red), DAPI (blue), and anti-GFP staining of GFP-cre (green, yellow with NeuN colocalization).

    See Abreview

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GFP antibody (ab290)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GFP antibody (ab290)This image is courtesy of an anonymous Abreview

    ab290 staining dog hearts (Adv-GFP injection) tissue sections by IHC-P.  Sections were PFA fixed and subjected to heat mediated antigen retrieval in citric acid (Ph6.0, 0.05% Tween20) prior to blocking with 10% serum for 30 mins at 37°C.  The primary antibody was diluted 1/1000 in PBS and incubated with the sample for 1 hour at 25°C.  A HRP conjugated secondary like  Goat Anti-Rabbit IgG H&L (HRP) (ab205718) was used.

    See Abreview

  • Immunocytochemistry - Anti-GFP antibody (ab290)
    Immunocytochemistry - Anti-GFP antibody (ab290)

    Immunofluorescence images showing similar localization of Yes-GFP (first 10 aa's of Yes PTK fused to the N-terminus of GFP) to full length Yes PTK. A: Distribution of Yes detected using mouse anti-Yes Ab followed by Texas Red-conjugated anti-mouse Ab. B: Chimeric GFP's detected using rabbit anti-GFP Ab (Abcam ab290) followed by FITC-conjugated anti-rabbit Ab.

    Image kindly provided by L.G. Berthiaume. Taken from J. McCabe and L.G. Berthiaume, Functional Roles for Fatty Acylated Amino-terminal Domains in Subcellular Localization, Molecular Biology of the Cell 10:3771-3786, 1999

  • Immunoprecipitation - Anti-GFP antibody (ab290)
    Immunoprecipitation - Anti-GFP antibody (ab290)This image is courtesy of an Abreview submitted by William Hung

    ab290 immunoprecipitating GFP in HEK293 nuclear lysate expressing GFP. 20µg of lysate was incubated with primary antibody (1 µg/mg lysate) and matrix (Protein G) for 16 hours at 4°C in AFC low salt buffer. For western blotting ab290 (1/5000) was used to confirm successful immunoprecipation.

    Lane 1: HEK293 nuclear lysate expressing GFP input.
    Lane 2: IP of HEK293 nuclear lysate expressing GFP.
    Lane 3: Cells with no GFP.

    See Abreview

  • Immunocytochemistry - Anti-GFP antibody (ab290)
    Immunocytochemistry - Anti-GFP antibody (ab290)

    ab290 staining GFP in GFP-transfected NIH3T3 cells. The cells were fixed with 4% formaldehyde (10min) and then blocked in 1% BSA / 0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated with ab290 at 1/200 dilution overnight at +4°C followed by incubation with Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150081), for 1 hour, at 1μg/ml.

    Under identical experimental conditions, when compared to the basal level of GFP expression in transfected NIH3T3 cells, the cells upon which ab290 was applied gave a stronger signal in the 488 channel, indicating that ab290 is binding to GFP and therefore eliciting signal amplification.

    ab290 was also applied to non-GFP-transfected NIH3T3 cells, which produced no positive staining, indicating specificity for GFP. Nuclear DNA was labelled with 1.43μM DAPI (blue).

  • Immunoprecipitation - Anti-GFP antibody (ab290)
    Immunoprecipitation - Anti-GFP antibody (ab290)This image is courtesy of an Abreview submitted by Vladimir Milenkovic

    ab290 immunoprecipitate in human HEK293 cells transfected with Annexin1-GFP. 25µg of cell lysate was incubated with the primary antibody and matrix (Protein G) in 1% TX-100, 10% glycerol, 1X PBS for 16 hours at 4°C. For Western blotting anti-rabbit HRP conjugated secondary antibody was used at a dilution at 1/5000.

    Lane 1: Lysate of HEK293 cells expressing Annexin1-GFP fusion protein.
    Lane 2: IP with anti-GFP.
    Lane 3: Not bound fraction.

    See Abreview

  • Immunocytochemistry - Anti-GFP antibody (ab290)
    Immunocytochemistry - Anti-GFP antibody (ab290)This image is courtesy of an anonymous Abreview

    ab290 staining GFP in U2OS cells expressing TRF2-GFP fusion protein by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with formaldehyde, permeabilized with NP40 and blocked with 3% BSA for 1 hour at 21°C. Samples were incubated with the primary antibody (1/1000 in PBS + 3% BSA) for 12 hours at 4°C. An Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at a dilution of 1/500 was used as the secondary antibody.

    Green - GFP.
    Blue - DAPI.

    See Abreview

  • Western blot - Anti-GFP antibody (ab290)
    Western blot - Anti-GFP antibody (ab290)
    Anti-GFP antibody (ab290) at 1/2500 dilution + Recombinant A. victoria GFP protein (ab84191) at 0.01 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Observed band size: 27 kDa why is the actual band size different from the predicted?


    Exposure time: 30 seconds


    Secondary antibody - goat anti-rabbit HRP preadsorbed (ab97080)

  • Western blot - Anti-GFP antibody (ab290)
    Western blot - Anti-GFP antibody (ab290)This image is courtesy of an anonymous Abreview
    All lanes : Anti-GFP antibody (ab290) at 1/5000 dilution

    Lane 1 : LNCaP whole cell lysate - pEGFP empty vector
    Lane 2 : LNCaP whole cell lysate - pEGFP-PKD1 transfected

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : HRP-conjugated goat anti-rabbit IgG at 1/10000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Exposure time: 10 seconds


    Blocked with 5% milk for 1 hour at 23°C.

    Incubated with the primary antibody for 16 hours at 4°C.

    See Abreview

  • Western blot - Anti-GFP antibody (ab290)
    Western blot - Anti-GFP antibody (ab290)This image is courtesy of an Abreview submitted by S Houtman
    All lanes : Anti-GFP antibody (ab290) at 1/5000 dilution

    Lane 1 : COS7 whole cell lysate - transfected with GFP-Eml4
    Lane 2 : COS7 whole cell lysate - transfected with GFP

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : HRP-conjugated pig anti-rabbit IgG at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Observed band size: 30 kDa why is the actual band size different from the predicted?


    Exposure time: 10 seconds


    Blocked with 5% milk for 1 hour at 20°C.

    Incubated with the primary antibody for 18 hours at 4°C in TBS containing 2% milk and 1% Tween.

    Predicted MW of Eml4 ~ 120 kDa.

    See Abreview

Protocols

  • Immunocytochemistry/ Immunofluorescence

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (2676)

Publishing research using ab290? Please let us know so that we can cite the reference in this datasheet.

ab290 has been referenced in 2676 publications.

  • Nilsson-Payant BE  et al. Reduced Nucleoprotein Availability Impairs Negative-Sense RNA Virus Replication and Promotes Host Recognition. J Virol 95:N/A (2021). PubMed: 33568513
  • Zahavi EE  et al. Combined kinesin-1 and kinesin-3 activity drives axonal trafficking of TrkB receptors in Rab6 carriers. Dev Cell 56:494-508.e7 (2021). PubMed: 33571451
  • Winkelsas AM  et al. Targeting the 5' untranslated region of SMN2 as a therapeutic strategy for spinal muscular atrophy. Mol Ther Nucleic Acids 23:731-742 (2021). PubMed: 33575118
  • Yoo H  et al. Down-regulation of habenular calcium-dependent secretion activator 2 induces despair-like behavior. Sci Rep 11:3700 (2021). PubMed: 33580180
  • Helmstadter KG  et al. CaMKII and PKA-dependent phosphorylation co-regulate nuclear localization of HDAC4 in adult cardiomyocytes. Basic Res Cardiol 116:11 (2021). PubMed: 33590335
View all Publications for this product

Customer reviews and Q&As

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1-10 of 268 Abreviews or Q&A

Western blot abreview for Anti-GFP antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Mouse Recombinant protein (HEK293T cells over expressing mouse 5-HT6R)
Loading amount
50 µg
Specification
HEK293T cells over expressing mouse 5-HT6R
Gel Running Conditions
Reduced Denaturing (8% Tris-Glycine gel)
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 22°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Mar 21 2013

Immunohistochemistry (PFA perfusion fixed frozen sections) abreview for Anti-GFP antibody - ChIP Grade

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Mouse Tissue sections (Brain)
Antigen retrieval step
None
Permeabilization
Yes - 0.2% Triton X
Specification
Brain
Blocking step
Serum as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 5% · Temperature: 22°C
Fixative
Paraformaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Mr. J. Y. Seo

Verified customer

Submitted Mar 27 2018

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-GFP antibody

Average
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Colon)
Specification
Colon
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 10 mM Citrate
Permeabilization
No
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Feb 12 2013

Immunocytochemistry/ Immunofluorescence abreview for Anti-GFP antibody - ChIP Grade

Excellent
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (U2oS)
Permeabilization
Yes - 0.1% Tween
Specification
U2oS
Blocking step
BSA as blocking agent for 45 minute(s) · Concentration: 5% · Temperature: 22°C
Fixative
Paraformaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Aug 06 2012

Western blot abreview for Anti-GFP antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Mouse Cell lysate - nuclear (mouse neurons)
Gel Running Conditions
Reduced Denaturing (4-12%)
Loading amount
40 µg
Specification
mouse neurons
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Jan 20 2022

Western blot abreview for Anti-GFP antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Mouse Cell lysate - nuclear (Cell line)
Gel Running Conditions
Reduced Denaturing
Loading amount
68 µg
Specification
Cell line
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Oct 12 2021

Western blot abreview for Anti-GFP antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Mouse Cell lysate - nuclear (cell line)
Gel Running Conditions
Reduced Denaturing (Gradient gel)
Loading amount
76 µg
Specification
cell line
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Sep 20 2021

Immunocytochemistry/ Immunofluorescence abreview for Anti-GFP antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HeLa)
Permeabilization
Yes - 0.1% Triton in PBS (5 mins)
Specification
HeLa
Blocking step
BSA as blocking agent for 15 minute(s) · Concentration: 1% · Temperature: 25°C
Fixative
Paraformaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Feb 19 2021

Western blot abreview for Anti-GFP antibody

Good
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Chinese hamster Cell lysate - whole cell (Chinese hamster ovary cells)
Gel Running Conditions
Reduced Denaturing (4-12% gel)
Loading amount
25 µg
Specification
Chinese hamster ovary cells
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 27°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Chansik Yoon

Verified customer

Submitted Feb 01 2021

Question

Does your antibody anti-GFP ab290 also recognize turbo-GFP?

Read More

Abcam community

Verified customer

Asked on Feb 19 2013

Answer

To our knowledge the detection of turbo-GFP has not been tested with ab290. Turbo-GFP is an improved variant of the green fluorescent protein CopGFP cloned from copepod Pontellina plumata , which is a different organism that the classical/original GFP which is from aquorea victoria jelly fish. The Aequorea victoria GFP (http://www.uniprot.org/uniprot/P42212) only has 26% homology with copepod GFP (http://www.uniprot.org/uniprot/Q6WV12), so we would not expect ab290 to recognize turbo-GPF or cop-GFP.

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Answered on Feb 19 2013

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