Recombinant Anti-GIRK2 antibody [EPR23841-83] (ab259909)
![Immunohistochemistry (Frozen sections) - Anti-GIRK2 antibody [EPR23841-83] (ab259909)](https://www.abcam.com/ps/products/259/ab259909/Images/ab259909-489819-anti-girk2-antibody-epr23841-83-immunohistochemistry-frozen-normal-substantia-nigra-human.jpg "Immunohistochemistry (Frozen sections) - Anti-GIRK2 antibody [EPR23841-83] (ab259909)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR23841-83] to GIRK2
- Suitable for: WB, IP, IHC-P, IHC-Fr
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-GIRK2 antibody [EPR23841-83]
See all GIRK2 primary antibodies -
Description
Rabbit monoclonal [EPR23841-83] to GIRK2 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IP, IHC-P, IHC-Frmore details
Unsuitable for: Flow Cyt (Intra) -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Mouse, rat and human brain lysate. IHC-P: Human cerebrum and insulinoma tissue. Mouse and rat cerebellum. IHC-Fr: Mouse and rat cerebral cortex, cerebellum and hippocampus tissue, Frozen human normal substantia nigra tissue. IP: Mouse brain tissue lysate.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59.94% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR23841-83 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab259909 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/1000. Predicted molecular weight: 48 kDa.
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| IP |
1/30.
|
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| IHC-P |
1/200. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
|
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| IHC-Fr |
Use a concentration of 0.05 µg/ml.
Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20) |
| Notes |
|---|
|
WB
1/1000. Predicted molecular weight: 48 kDa. |
|
IP
1/30. |
|
IHC-P
1/200. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
|
IHC-Fr
Use a concentration of 0.05 µg/ml. Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20) |
Target
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Function
This potassium channel may be involved in the regulation of insulin secretion by glucose and/or neurotransmitters acting through G-protein-coupled receptors. Inward rectifier potassium channels are characterized by a greater tendency to allow potassium to flow into the cell rather than out of it. Their voltage dependence is regulated by the concentration of extracellular potassium; as external potassium is raised, the voltage range of the channel opening shifts to more positive voltages. The inward rectification is mainly due to the blockage of outward current by internal magnesium. -
Tissue specificity
Most abundant in cerebellum, and to a lesser degree in islets and exocrine pancreas. -
Involvement in disease
Keppen-Lubinsky syndrome -
Sequence similarities
Belongs to the inward rectifier-type potassium channel (TC 1.A.2.1) family. KCNJ6 subfamily. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 3763 Human
- Entrez Gene: 16522 Mouse
- Entrez Gene: 25743 Rat
- Omim: 600877 Human
- SwissProt: P48051 Human
- SwissProt: P48542 Mouse
- SwissProt: P48550 Rat
- Unigene: 626242 Human
see all -
Alternative names
- inwardly rectifying subfamily J member 6 antibody
- BIR1 antibody
- G protein activated inward rectifier potassium channel 2 antibody
see all
Images
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Immunohistochemistry (Frozen sections) - Anti-GIRK2 antibody [EPR23841-83] (ab259909)
IHC image of GIRK2 staining in a section of frozen human normal substantia nigra performed on a Leica Biosystems BOND® RX instrument using the standard protocol. The section was fixed in 10% paraformaldehyde (10 min) prior to staining. The section was incubated with ab259909, 0.05 μg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre.
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![Western blot - Anti-GIRK2 antibody [EPR23841-83] (ab259909)](https://www.abcam.com/ps/products/259/ab259909/Images/ab259909-459590-anti-girk2-antibody-epr2384183-western-blot-lysate.jpg)
Western blot - Anti-GIRK2 antibody [EPR23841-83] (ab259909)All lanes : Anti-GIRK2 antibody [EPR23841-83] (ab259909) at 1/1000 dilution
Lane 1 : Mouse brain tissue lysate at 20 µg
Lane 2 : Mouse liver tissue lysate at 40 µg
Lane 3 : Rat brain tissue lysate at 20 µg
Lane 4 : Rat liver tissue lysate at 40 µg
Secondary
All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Predicted band size: 48 kDaBlocking and diluting buffer and concentration: 5% NFDM/TBST
Samples are non-boiled as boiling may cause protein aggregates.
Negative control: liver (PMID: 8929423).
Exposure time: 5.5 secs.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - (ab259909)Immunohistochemical analysis of paraffin-embedded human cerebrum tissue labelling GIRK2 with ab259909 at 1/200 (2.345 µg/ml) dilution followed by ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). The section was incubated with ab259909 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Cytoplasmic staining on human cerebrum (PMID: 8642402).
Secondary antibody only control: Secondary antibody is ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
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Immunohistochemistry (Frozen sections) - (ab259909)Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen mouse cerebral cortex tissue labeling GIRK2 with ab259909 at 1/100 (4.69 µg/ml) dilution followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution (Green). The nuclear counterstain was DAPI (Blue).
Cytoplasmic staining on mouse cerebral cortex (PMID: 8929423) is observed.
Secondary antibody control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.
Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).
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Immunoprecipitation - (ab259909)GIRK2 was immunoprecipitated from 0.35 mg mouse brain tissue lysate 10 µg with ab259909 at 1/30 dilution (2 µg in 0.35 mg lysates). Western blot was performed on the immunoprecipitate using ab259909 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution.
Lane 1: Mouse brain tissue lysate 10 µg.
Lane 2: ab259909 IP in mouse brain tissue lysate.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab259909 in mouse brain tissue lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 23 seconds.
Input sample is non-boiled as boiling may cause protein aggregates.The expression of truncated GIRK2 proteins observed around 40KD is consistent with what has been described in the literature (PMID:28951616).
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![Western blot - Anti-GIRK2 antibody [EPR23841-83] (ab259909)](https://www.abcam.com/ps/products/259/ab259909/Images/ab259909-459591-anti-girk2-antibody-epr2384183-western-blot-lysate1.jpg)
Western blot - Anti-GIRK2 antibody [EPR23841-83] (ab259909)All lanes : Anti-GIRK2 antibody [EPR23841-83] (ab259909) at 1/1000 dilution
Lane 1 : Human brain tissue lysate at 20 µg
Lane 2 : Human liver tissue lysate at 40 µg
Secondary
All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Predicted band size: 48 kDa
Observed band size: 48,40,35 kDa why is the actual band size different from the predicted?Blocking and diluting buffer and concentration: 5% NFDM/TBST
Molecular weight around 40kDa, cleaved forms. (PMID:28951616).
Samples are non-boiled as boiling may cause protein aggregates.
Negative control: liver (PMID: 8929423).
Exposure time: 5.5 seconds.
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - (ab259909)Immunohistochemical analysis of paraffin-embedded human insulinoma tissue labelling GIRK2 with ab259909 at 1/200 (2.345 µg/ml) dilution followed by ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). The section was incubated with ab259909 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Cytoplasmic staining on human insulinoma (PMID: 7592809).
Secondary antibody only control: Secondary antibody is ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - (ab259909)Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue labelling GIRK2 with ab259909 at 1/200 (2.345 µg/ml) dilution followed by ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). The section was incubated with ab259909 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Postive staining predominantly on granular layer of mouse cerebellum (PMID: 8642402, PMID: 9023358 ).
Secondary antibody only control: Secondary antibody is ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - (ab259909)Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue labelling GIRK2 with ab259909 at 1/200 (2.345 µg/ml) dilution followed by ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). The section was incubated with ab259909 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Postive staining predominantly on granular layer of rat cerebellum (PMID: 8642402, PMID: 9023358).
Secondary antibody only control: Secondary antibody is ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - (ab259909)Immunohistochemical analysis of paraffin-embedded human liver tissue labelling GIRK2 with ab259909 at 1/200 (2.345 µg/ml) dilution followed by ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). The section was incubated with ab259909 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Negative control: No staining on human liver (PMID: 7592809).
Secondary antibody only control: Secondary antibody is ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
-
![Immunohistochemistry (Frozen sections) - Anti-GIRK2 antibody [EPR23841-83] (ab259909)](https://www.abcam.com/ps/products/259/ab259909/Images/ab259909-489820-anti-girk2-antibody-epr23841-83-immunohistochemistry-frozen-liver-human.jpg)
Immunohistochemistry (Frozen sections) - Anti-GIRK2 antibody [EPR23841-83] (ab259909)Negative tissue control image: IHC image of GIRK2 staining in a section of frozen human normal liver performed on a Leica Biosystems BOND® RX instrument using the standard protocol. The section was fixed in 10% paraformaldehyde (10 min) prior to staining. The section was incubated with ab259909, 0.05 μg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre.
-
Immunohistochemistry (Frozen sections) - (ab259909)Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen mouse cerebellum tissue labeling GIRK2 with ab259909 at 1/100 (4.69 µg/ml) dilution followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution (Green). The nuclear counterstain was DAPI (Blue).
Cytoplasmic staining on mouse cerebellum (PMID: 8929423) is observed.
Secondary antibody control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.
Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).
-
Immunohistochemistry (Frozen sections) - (ab259909)Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen mouse hippocampus tissue labeling GIRK2 with ab259909 at 1/100 (4.69 µg/ml) dilution followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution (Green). The nuclear counterstain was DAPI (Blue).
Cytoplasmic staining on mouse hippocampus (PMID: 8929423) is observed.
Secondary antibody control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.
Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).
-
Immunohistochemistry (Frozen sections) - (ab259909)Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen mouse liver tissue labeling GIRK2 with ab259909 at 1/100 (4.69 µg/ml) dilution followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution (Green). The nuclear counterstain was DAPI (Blue).
Negative control: No staining on mouse liver (PMID: 7592809) is observed.
Secondary antibody control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)at 1/1000 dilution.
Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).
-
Immunohistochemistry (Frozen sections) - (ab259909)Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen rat cerebral cortex tissue labeling GIRK2 with ab259909 at 1/100 (4.69 µg/ml) dilution followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution (Green). The nuclear counterstain was DAPI (Blue).
Cytoplasmic staining on rat cerebral cortex (PMID: 8929423) is observed.
Secondary antibody control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.
Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).
-
Immunohistochemistry (Frozen sections) - (ab259909)Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen rat cerebellum tissue labeling GIRK2 with ab259909 at 1/100 (4.69 µg/ml) dilution followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution (Green). The nuclear counterstain was DAPI (Blue).
Cytoplasmic staining on rat cerebellum (PMID: 8929423) is observed.
Secondary antibody control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.
Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).
-
Immunohistochemistry (Frozen sections) - (ab259909)Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen rat hippocampus tissue labeling GIRK2 with ab259909 at 1/100 (4.69 µg/ml) dilution followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution (Green). The nuclear counterstain was DAPI (Blue).
Cytoplasmic staining on rat hippocampus (PMID: 8929423) is observed.
Secondary antibody control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.
Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).
-
Immunohistochemistry (Frozen sections) - (ab259909)Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen rat liver tissue labeling GIRK2 with ab259909 at 1/100 (4.69 µg/ml) dilution followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution (Green). The nuclear counterstain was DAPI (Blue).
Negative control: No staining on rat liver (PMID: 7592809) is observed.
Secondary antibody control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)at 1/1000 dilution.
Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).
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(ab259909)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (0)
ab259909 has not yet been referenced specifically in any publications.