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    products/primary-antibodies/gli1-antibody-ab217326.pdf

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Epigenetics and Nuclear Signaling Transcription Domain Families Zinc Finger
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Anti-Gli1 antibody (ab217326)

  • Datasheet
  • SDS
Reviews (2) Submit a question References (3)

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Western blot - Anti-Gli1 antibody (ab217326)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Gli1 antibody (ab217326)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Gli1 antibody (ab217326)
  • Western blot - Anti-Gli1 antibody (ab217326)

Key features and details

  • Rabbit polyclonal to Gli1
  • Suitable for: WB, IHC-P
  • Reacts with: Mouse, Human
  • Isotype: IgG

Get better batch-to-batch reproducibility with a recombinant antibody

Product image
Anti-Gli1 antibody [EPR4523] (ab134906)
  • Research with confidence – consistent and reproducible results with every batch
  • Long-term and scalable supply – powered by recombinant technology for fast production
  • Success from the first experiment – confirmed specificity through extensive validation
  • Ethical standards compliant – production is animal-free

Overview

  • Product name

    Anti-Gli1 antibody
    See all Gli1 primary antibodies
  • Description

    Rabbit polyclonal to Gli1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Recombinant fragment corresponding to Human Gli1 aa 750 to the C-terminus (C terminal).
    Database link: P08151

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control

    • WB: Recombinant human Gli1 protein; U87, MCF7, HeLa, SKOV, HT1080, COLO320 and HepG2 whole cell lysate (ab7900). IHC-P: Human intestinal cancer tissue.
  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.025% Sodium azide
    Constituents: 2.5% BSA, 0.1% Dibasic monohydrogen sodium phosphate, 0.45% Sodium chloride
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • Transcription
    • Domain Families
    • Zinc Finger
    • Neuroscience
    • Neurology process
    • Neural Signal Transduction
    • Stem Cells
    • Signaling Pathways
    • Hedgehog
    • Nuclear
    • Cancer
    • Oncoproteins/suppressors
    • Tumor suppressors
    • PTC & Wnt pathway
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Domain Families
    • Krueppel like factor

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Positive Controls

    • HeLa whole cell lysate (ab150035)
    • Hep G2 whole cell lysate (ab166833)
    • MCF7 whole cell lysate (ab29537)
    • HeLa whole cell lysate (ab29545)
  • Recombinant Protein

    • Recombinant Human Gli1 protein (ab152427)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab217326 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
Use a concentration of 0.1 - 0.5 µg/ml. Detects a band of approximately 118 kDa (predicted molecular weight: 118 kDa).

The detection limit for GLi1 is approximately 0.25 ng/lane under reducing conditions.

IHC-P
Use a concentration of 0.5 - 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Notes
WB
Use a concentration of 0.1 - 0.5 µg/ml. Detects a band of approximately 118 kDa (predicted molecular weight: 118 kDa).

The detection limit for GLi1 is approximately 0.25 ng/lane under reducing conditions.

IHC-P
Use a concentration of 0.5 - 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function

    Acts as a transcriptional activator. May regulate the transcription of specific genes during normal development. May play a role in craniofacial development and digital development, as well as development of the central nervous system and gastrointestinal tract. Mediates SHH signaling and thus cell proliferation and differentiation.
  • Tissue specificity

    Testis, myometrium and fallopian tube. Also expressed in the brain with highest expression in the cerebellum, optic nerve and olfactory tract.
  • Sequence similarities

    Belongs to the GLI C2H2-type zinc-finger protein family.
    Contains 5 C2H2-type zinc fingers.
  • Post-translational
    modifications

    Phosphorylated in vitro by ULK3.
  • Cellular localization

    Cytoplasm. Nucleus. Tethered in the cytoplasm by binding to SUFU. Activation and translocation to the nucleus is promoted by interaction with STK36. Phosphorylation by ULK3 may promote nuclear localization. Translocation to the nucleus is promoted by interaction with ZIC1.
  • Target information above from: UniProt accession P08151 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 2735 Human
    • Entrez Gene: 14632 Mouse
    • Omim: 165220 Human
    • SwissProt: P08151 Human
    • SwissProt: P47806 Mouse
    • Unigene: 632702 Human
    • Unigene: 391450 Mouse
    • Alternative names

      • Gli 1 antibody
      • GLI antibody
      • GLI family zinc finger 1 antibody
      • GLI Kruppel family member 1 antibody
      • gli1 antibody
      • GLI1_HUMAN antibody
      • Glioma associated oncogene 1 antibody
      • Glioma associated oncogene homolog 1 (zinc finger protein) antibody
      • Glioma associated oncogene homolog antibody
      • Glioma-associated oncogene antibody
      • Oncogene GLI antibody
      • Zfp 5 antibody
      • Zfp5 antibody
      • Zinc finger protein GLI 1 antibody
      • Zinc finger protein GLI1 antibody
      see all

    Images

    • Western blot - Anti-Gli1 antibody (ab217326)
      Western blot - Anti-Gli1 antibody (ab217326)
      All lanes : Anti-Gli1 antibody (ab217326) at 0.5 µg/ml

      Lane 1 : U87 whole cell lysate
      Lane 2 : MCF7 whole cell lysate
      Lane 3 : HeLa whole cell lysate
      Lane 4 : SKOV whole cell lysate
      Lane 5 : HT1080 whole cell lysate
      Lane 6 : COLO320 whole cell lysate
      Lane 7 : HepG2 whole cell lysate

      Lysates/proteins at 40 µg per lane.

      Predicted band size: 118 kDa

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Gli1 antibody (ab217326)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Gli1 antibody (ab217326)

      Immunohistochemical analysis of paraffin embedded mouse testis tissue staining Gli1 with ab217326 at 1µg/ml. DyLight®488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1/100 dilution. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution ) for 20 mins. DAPI was used as a nucelar counterstain (blue).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Gli1 antibody (ab217326)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Gli1 antibody (ab217326)

      Immunohistochemical analysis of paraffin-embedded human intestinal cancer tissue labeling Gil1 with ab217326 at 1 µg/mL.

    • Western blot - Anti-Gli1 antibody (ab217326)
      Western blot - Anti-Gli1 antibody (ab217326)
      Anti-Gli1 antibody (ab217326) at 0.5 µg/ml + recombinant human GLI1 protein, 5 ng

      Predicted band size: 118 kDa

    Protocols

    • Western blot protocols
    • Immunohistochemistry protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (3)

    Publishing research using ab217326? Please let us know so that we can cite the reference in this datasheet.

    ab217326 has been referenced in 3 publications.

    • Du W  et al. miR-367-3p downregulates Rab23 expression and inhibits Hedgehog signaling resulting in the inhibition of the proliferation, migration, and invasion of prostate cancer cells. Oncol Rep 46:N/A (2021). PubMed: 34278506
    • You X  et al. Galectin-1 promotes vasculogenic mimicry in gastric adenocarcinoma via the Hedgehog/GLI signaling pathway. Aging (Albany NY) 12:21837-21853 (2020). PubMed: 33170154
    • Zhao Z  et al. Degalactotigonin, a Natural Compound from Solanum nigrum L., Inhibits Growth and Metastasis of Osteosarcoma through GSK3ß Inactivation-Mediated Repression of the Hedgehog/Gli1 Pathway. Clin Cancer Res 24:130-144 (2018). PubMed: 28951519

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-2 of 2 Abreviews or Q&A

    Immunohistochemistry (Frozen sections) abreview for Anti-Gli1 antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Mouse Tissue sections (Whole Embryo (E12.5 LEFT VENTRICLE/CARDIAC))
    Permeabilization
    Yes - 0.3% Triton X-100
    Specification
    Whole Embryo (E12.5 LEFT VENTRICLE/CARDIAC)
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
    Fixative
    Paraformaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Lindsey Fitzsimons

    Verified customer

    Submitted Sep 27 2021

    Immunohistochemistry (PFA perfusion fixed frozen sections) abreview for Anti-Gli1 antibody

    Average
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (PFA perfusion fixed frozen sections)
    Sample
    Pig Tissue sections (Lung)
    Antigen retrieval step
    None
    Permeabilization
    Yes - Triton X-100
    Specification
    Lung
    Blocking step
    Invitrogen IHC/ICC Blocking Buffer as blocking agent for 1 hour(s) and 0 minute(s) · Temperature: 23°C
    Fixative
    Paraformaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Ross Lampe

    Verified customer

    Submitted Jan 18 2021

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