Anti-Gli1 antibody (ab217326)
Key features and details
- Rabbit polyclonal to Gli1
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
- Long-term and scalable supply – powered by recombinant technology for fast production
- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
-
Product name
Anti-Gli1 antibody
See all Gli1 primary antibodies -
Description
Rabbit polyclonal to Gli1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Recombinant fragment corresponding to Human Gli1 aa 750 to the C-terminus (C terminal).
Database link: P08151 -
Positive control
- WB: Recombinant human Gli1 protein; U87, MCF7, HeLa, SKOV, HT1080, COLO320 and HepG2 whole cell lysate (ab7900). IHC-P: Human intestinal cancer tissue.
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.025% Sodium azide
Constituents: 2.5% BSA, 0.1% Dibasic monohydrogen sodium phosphate, 0.45% Sodium chloride -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
-
Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab217326 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
Use a concentration of 0.1 - 0.5 µg/ml. Detects a band of approximately 118 kDa (predicted molecular weight: 118 kDa).
The detection limit for GLi1 is approximately 0.25 ng/lane under reducing conditions. |
|
| IHC-P |
Use a concentration of 0.5 - 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
| Notes |
|---|
|
WB
Use a concentration of 0.1 - 0.5 µg/ml. Detects a band of approximately 118 kDa (predicted molecular weight: 118 kDa). The detection limit for GLi1 is approximately 0.25 ng/lane under reducing conditions. |
|
IHC-P
Use a concentration of 0.5 - 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
-
Function
Acts as a transcriptional activator. May regulate the transcription of specific genes during normal development. May play a role in craniofacial development and digital development, as well as development of the central nervous system and gastrointestinal tract. Mediates SHH signaling and thus cell proliferation and differentiation. -
Tissue specificity
Testis, myometrium and fallopian tube. Also expressed in the brain with highest expression in the cerebellum, optic nerve and olfactory tract. -
Sequence similarities
Belongs to the GLI C2H2-type zinc-finger protein family.
Contains 5 C2H2-type zinc fingers. -
Post-translational
modificationsPhosphorylated in vitro by ULK3. -
Cellular localization
Cytoplasm. Nucleus. Tethered in the cytoplasm by binding to SUFU. Activation and translocation to the nucleus is promoted by interaction with STK36. Phosphorylation by ULK3 may promote nuclear localization. Translocation to the nucleus is promoted by interaction with ZIC1. - Information by UniProt
-
Database links
- Entrez Gene: 2735 Human
- Entrez Gene: 14632 Mouse
- Omim: 165220 Human
- SwissProt: P08151 Human
- SwissProt: P47806 Mouse
- Unigene: 632702 Human
- Unigene: 391450 Mouse
-
Alternative names
- Gli 1 antibody
- GLI antibody
- GLI family zinc finger 1 antibody
see all
Images
-
Western blot - Anti-Gli1 antibody (ab217326)All lanes : Anti-Gli1 antibody (ab217326) at 0.5 µg/ml
Lane 1 : U87 whole cell lysate
Lane 2 : MCF7 whole cell lysate
Lane 3 : HeLa whole cell lysate
Lane 4 : SKOV whole cell lysate
Lane 5 : HT1080 whole cell lysate
Lane 6 : COLO320 whole cell lysate
Lane 7 : HepG2 whole cell lysate
Lysates/proteins at 40 µg per lane.
Predicted band size: 118 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Gli1 antibody (ab217326)Immunohistochemical analysis of paraffin embedded mouse testis tissue staining Gli1 with ab217326 at 1µg/ml. DyLight®488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1/100 dilution. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution ) for 20 mins. DAPI was used as a nucelar counterstain (blue).
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Gli1 antibody (ab217326)Immunohistochemical analysis of paraffin-embedded human intestinal cancer tissue labeling Gil1 with ab217326 at 1 µg/mL.
-
Western blot - Anti-Gli1 antibody (ab217326)Anti-Gli1 antibody (ab217326) at 0.5 µg/ml + recombinant human GLI1 protein, 5 ng
Predicted band size: 118 kDa
Protocols
Datasheets and documents
-
SDS download
-
Datasheet download
References (3)
ab217326 has been referenced in 3 publications.
- Du W et al. miR-367-3p downregulates Rab23 expression and inhibits Hedgehog signaling resulting in the inhibition of the proliferation, migration, and invasion of prostate cancer cells. Oncol Rep 46:N/A (2021). PubMed: 34278506
- You X et al. Galectin-1 promotes vasculogenic mimicry in gastric adenocarcinoma via the Hedgehog/GLI signaling pathway. Aging (Albany NY) 12:21837-21853 (2020). PubMed: 33170154
- Zhao Z et al. Degalactotigonin, a Natural Compound from Solanum nigrum L., Inhibits Growth and Metastasis of Osteosarcoma through GSK3ß Inactivation-Mediated Repression of the Hedgehog/Gli1 Pathway. Clin Cancer Res 24:130-144 (2018). PubMed: 28951519