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    products/primary-antibodies/gli1-antibody-epr4523-ab134906.pdf

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Epigenetics and Nuclear Signaling Transcription Domain Families Zinc Finger
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RecombinantRabMAb

Recombinant Anti-Gli1 antibody [EPR4523] (ab134906)

  • Datasheet
  • SDS
Reviews (2)Q&A (1)References (20)

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Western blot - Anti-Gli1 antibody [EPR4523] (ab134906)
  • Western blot - Anti-Gli1 antibody [EPR4523] (ab134906)
  • Anti-Gli1 antibody [EPR4523] (ab134906)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR4523] to Gli1
  • Suitable for: WB
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

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Protein
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Recombinant Human Gli1 protein (ab152427)
Secondary
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Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

View more associated products

Overview

  • Product name

    Anti-Gli1 antibody [EPR4523]
    See all Gli1 primary antibodies
  • Description

    Rabbit monoclonal [EPR4523] to Gli1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
    Unsuitable for: Flow Cyt,ICC/IF,IHC-P or IP
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human Gli1 aa 1-100. The exact sequence is proprietary.

  • Positive control

    • RMS13 and A673 cell lysates; Human brain and testis tissues; A673 cells.
  • General notes

    This antibody was developed as part of a collaboration with the lab of Martin Fernandez-Zapico at the Mayo Clinic.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20ºC.
  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 40% Glycerol, 0.05% BSA, 59% PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR4523
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • Transcription
    • Domain Families
    • Zinc Finger
    • Neuroscience
    • Neurology process
    • Neural Signal Transduction
    • Stem Cells
    • Signaling Pathways
    • Hedgehog
    • Nuclear
    • Cancer
    • Oncoproteins/suppressors
    • Tumor suppressors
    • PTC & Wnt pathway
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Domain Families
    • Krueppel like factor

Associated products

  • Alternative Versions

    • Anti-Gli1 antibody [EPR4523] - BSA and Azide free (ab240088)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Recombinant Protein

    • Recombinant Human Gli1 protein (ab152427)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab134906 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB (2)
1/100 - 1/1000. Predicted molecular weight: 118 kDa.
Notes
WB
1/100 - 1/1000. Predicted molecular weight: 118 kDa.
Application notes
Is unsuitable for Flow Cyt,ICC/IF,IHC-P or IP.

Target

  • Function

    Acts as a transcriptional activator. May regulate the transcription of specific genes during normal development. May play a role in craniofacial development and digital development, as well as development of the central nervous system and gastrointestinal tract. Mediates SHH signaling and thus cell proliferation and differentiation.
  • Tissue specificity

    Testis, myometrium and fallopian tube. Also expressed in the brain with highest expression in the cerebellum, optic nerve and olfactory tract.
  • Sequence similarities

    Belongs to the GLI C2H2-type zinc-finger protein family.
    Contains 5 C2H2-type zinc fingers.
  • Post-translational
    modifications

    Phosphorylated in vitro by ULK3.
  • Cellular localization

    Cytoplasm. Nucleus. Tethered in the cytoplasm by binding to SUFU. Activation and translocation to the nucleus is promoted by interaction with STK36. Phosphorylation by ULK3 may promote nuclear localization. Translocation to the nucleus is promoted by interaction with ZIC1.
  • Target information above from: UniProt accession P08151 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 2735 Human
    • Omim: 165220 Human
    • SwissProt: P08151 Human
    • Unigene: 632702 Human
    • Alternative names

      • Gli 1 antibody
      • GLI antibody
      • GLI family zinc finger 1 antibody
      • GLI Kruppel family member 1 antibody
      • gli1 antibody
      • GLI1_HUMAN antibody
      • Glioma associated oncogene 1 antibody
      • Glioma associated oncogene homolog 1 (zinc finger protein) antibody
      • Glioma associated oncogene homolog antibody
      • Glioma-associated oncogene antibody
      • Oncogene GLI antibody
      • Zfp 5 antibody
      • Zfp5 antibody
      • Zinc finger protein GLI 1 antibody
      • Zinc finger protein GLI1 antibody
      see all

    Images

    • Western blot - Anti-Gli1 antibody [EPR4523] (ab134906)
      Western blot - Anti-Gli1 antibody [EPR4523] (ab134906)
      Anti-Gli1 antibody [EPR4523] (ab134906) at 1/1000 dilution (purified) + RMS13 cell lysate at 10 µg

      Secondary
      HRP goat anti-rabbit (H+L) at 1/1000 dilution

      Predicted band size: 118 kDa
      Observed band size: 150 kDa why is the actual band size different from the predicted?



      Blocking buffer: 5% NFDM/TBST

      Dilution buffer: 5% NFDM/TBST

    • Western blot - Anti-Gli1 antibody [EPR4523] (ab134906)
      Western blot - Anti-Gli1 antibody [EPR4523] (ab134906)
      All lanes : Anti-Gli1 antibody [EPR4523] (ab134906) at 1/1000 dilution (unpurified)

      Lane 1 : RMS13 cell lysate
      Lane 2 : A673 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP-conjugated goat anti-rabbit IgG polyclonal at 1/2000 dilution

      Predicted band size: 118 kDa

    • Anti-Gli1 antibody [EPR4523] (ab134906)
      Anti-Gli1 antibody [EPR4523] (ab134906)

    Protocols

    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (20)

    Publishing research using ab134906? Please let us know so that we can cite the reference in this datasheet.

    ab134906 has been referenced in 20 publications.

    • Liu T  et al. MicroRNA-26a inhibits cell proliferation and invasion by targeting FAM98A in breast cancer. Oncol Lett 21:367 (2021). PubMed: 33747224
    • Xia Y  et al. MIRLET7BHG promotes hepatocellular carcinoma progression by activating hepatic stellate cells through exosomal SMO to trigger Hedgehog pathway. Cell Death Dis 12:326 (2021). PubMed: 33771969
    • Kim SH  et al. Identification of recurrent FHL2-GLI2 oncogenic fusion in sclerosing stromal tumors of the ovary. Nat Commun 11:44 (2020). PubMed: 31896750
    • Qiu S  et al. LncRNA EGOT decreases breast cancer cell viability and migration via inactivation of the Hedgehog pathway. FEBS Open Bio 10:817-826 (2020). PubMed: 32150666
    • Hua J  et al. Small Molecule-Based Strategy Promotes Nucleus Pulposus Specific Differentiation of Adipose-Derived Mesenchymal Stem Cells. Mol Cells 42:661-671 (2019). PubMed: 31564076
    View all Publications for this product

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-3 of 3 Abreviews or Q&A

    Western blot abreview for Anti-Gli1 antibody [EPR4523]

    Poor
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Cell lysate - whole cell (liver)
    Gel Running Conditions
    Reduced Denaturing
    Loading amount
    50 µg
    Specification
    liver
    Blocking step
    licor blocking solution as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 25°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted May 21 2019

    Western blot abreview for Anti-Gli1 antibody [EPR4523]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (Glioblastoma primary cells)
    Loading amount
    25 µg
    Specification
    Glioblastoma primary cells
    Gel Running Conditions
    Reduced Denaturing (gradient gel 5-15%)
    Blocking step
    Milk as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 20°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Nov 21 2012

    Question

    Dear tech,

    I would like to know whether ab134906 specifically recognizes Gli 1 but does not cross react with Gli 2 or Gli 3. Would you please check it?

    Thank you for your help!

    Read More

    Abcam community

    Verified customer

    Asked on Dec 05 2012

    Answer

    Thank you for contacting us.

    We have not tested whether ab134906 will cross-react with Gli 2 or Gli 3. However, based on the sequence homology between the immunogen and the protein Gli 2 or Gli 3, cross-reactivity is not likely. Though, It can only be confirmed in actual experiment.

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Read More

    Abcam Scientific Support

    Answered on Dec 05 2012

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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