Recombinant Anti-Gli1 antibody [EPR4523] (ab134906)
![Western blot - Anti-Gli1 antibody [EPR4523] (ab134906)](https://www.abcam.com/ps/products/134/ab134906/Images/ab134906-239393-anti-gli1-antibody-epr4523-western-blot.jpg "Western blot - Anti-Gli1 antibody [EPR4523] (ab134906)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR4523] to Gli1
- Suitable for: WB
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-Gli1 antibody [EPR4523]
See all Gli1 primary antibodies -
Description
Rabbit monoclonal [EPR4523] to Gli1 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details
Unsuitable for: Flow Cyt,ICC/IF,IHC-P or IP -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human Gli1 aa 1-100. The exact sequence is proprietary.
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Positive control
- RMS13 and A673 cell lysates; Human brain and testis tissues; A673 cells.
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General notes
This antibody was developed as part of a collaboration with the lab of Martin Fernandez-Zapico at the Mayo Clinic.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20ºC. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol, 0.05% BSA, 59% PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR4523 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab134906 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | (2) |
1/100 - 1/1000. Predicted molecular weight: 118 kDa.
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| Notes |
|---|
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WB
1/100 - 1/1000. Predicted molecular weight: 118 kDa. |
Target
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Function
Acts as a transcriptional activator. May regulate the transcription of specific genes during normal development. May play a role in craniofacial development and digital development, as well as development of the central nervous system and gastrointestinal tract. Mediates SHH signaling and thus cell proliferation and differentiation. -
Tissue specificity
Testis, myometrium and fallopian tube. Also expressed in the brain with highest expression in the cerebellum, optic nerve and olfactory tract. -
Sequence similarities
Belongs to the GLI C2H2-type zinc-finger protein family.
Contains 5 C2H2-type zinc fingers. -
Post-translational
modificationsPhosphorylated in vitro by ULK3. -
Cellular localization
Cytoplasm. Nucleus. Tethered in the cytoplasm by binding to SUFU. Activation and translocation to the nucleus is promoted by interaction with STK36. Phosphorylation by ULK3 may promote nuclear localization. Translocation to the nucleus is promoted by interaction with ZIC1. - Information by UniProt
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Database links
- Entrez Gene: 2735 Human
- Omim: 165220 Human
- SwissProt: P08151 Human
- Unigene: 632702 Human
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Alternative names
- Gli 1 antibody
- GLI antibody
- GLI family zinc finger 1 antibody
see all
Images
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Western blot - Anti-Gli1 antibody [EPR4523] (ab134906)Anti-Gli1 antibody [EPR4523] (ab134906) at 1/1000 dilution (purified) + RMS13 cell lysate at 10 µg
Secondary
HRP goat anti-rabbit (H+L) at 1/1000 dilution
Predicted band size: 118 kDa
Observed band size: 150 kDa why is the actual band size different from the predicted?Blocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST
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![Western blot - Anti-Gli1 antibody [EPR4523] (ab134906)](https://www.abcam.com/ps/products/134/ab134906/Images/ab134906-149030-anti-gli1-antibody-epr4523-western-blot.jpg)
Western blot - Anti-Gli1 antibody [EPR4523] (ab134906)All lanes : Anti-Gli1 antibody [EPR4523] (ab134906) at 1/1000 dilution (unpurified)
Lane 1 : RMS13 cell lysate
Lane 2 : A673 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP-conjugated goat anti-rabbit IgG polyclonal at 1/2000 dilution
Predicted band size: 118 kDa -
![Anti-Gli1 antibody [EPR4523] (ab134906)](https://www.abcam.com/ps/products/134/ab134906/Images/ab134906-7-benefits-of-recombinant-antibodies.png)
Anti-Gli1 antibody [EPR4523] (ab134906)
Datasheets and documents
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SDS download
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Datasheet download
References (30)
ab134906 has been referenced in 30 publications.
- Kotulova J et al. 2‑Cl‑IB‑MECA regulates the proliferative and drug resistance pathways, and facilitates chemosensitivity in pancreatic and liver cancer cell lines. Int J Mol Med 49:N/A (2022). PubMed: 35039871
- Cui X et al. Collagen type IV alpha 1 (COL4A1) silence hampers the invasion, migration and epithelial-mesenchymal transition (EMT) of gastric cancer cells through blocking Hedgehog signaling pathway. Bioengineered 13:8972-8981 (2022). PubMed: 35297303
- Zhu Y et al. METTL3-mediated m6A modification of STEAP2 mRNA inhibits papillary thyroid cancer progress by blocking the Hedgehog signaling pathway and epithelial-to-mesenchymal transition. Cell Death Dis 13:358 (2022). PubMed: 35436987
- Zhao J et al. CAFs-derived SCUBE1 promotes malignancy and stemness through the Shh/Gli1 pathway in hepatocellular carcinoma. J Transl Med 20:520 (2022). PubMed: 36348351
- Zhang S et al. The PROTAC selectively degrading Bcl-xL represents a novel Hedgehog pathway inhibitor with capacity of combating resistance to Smoothened inhibitors while sparing bone growth. Theranostics 12:7476-7490 (2022). PubMed: 36438482