Recombinant Anti-GLP-1R antibody [EPR21819] (ab218532)
![Western blot - Anti-GLP-1R antibody [EPR21819] (ab218532)](https://www.abcam.com/ps/products/218/ab218532/Images/ab218532-478986-AntiGLP1R-antibody-Western-Blot.png "Western blot - Anti-GLP-1R antibody [EPR21819] (ab218532)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR21819] to GLP-1R
- Suitable for: IHC-Fr, WB, IHC-P
- Reacts with: Mouse, Rat
Related conjugates and formulations
Overview
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Product name
Anti-GLP-1R antibody [EPR21819]
See all GLP-1R primary antibodies -
Description
Rabbit monoclonal [EPR21819] to GLP-1R -
Host species
Rabbit -
Specificity
Rat species is recommended based on IHC-P / IHC-Fr results, we do not guarantee WB for rat.
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Tested applications
Suitable for: IHC-Fr, WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Rat -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Beta-TC-6 whole cell lysate. IHC-P: Mouse and rat pancreas tissue. IHC-Fr: Mouse and rat pancreas tissue.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR21819 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab218532 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-Fr |
1/500.
Heat-mediated antigen retrieval using Tris-EDTA buffer (pH9.0) (ab94681) |
|
| WB |
1/1000. Predicted molecular weight: 53 kDa.
Rat species is recommended based on IHC-P/ IHC-Fr results, we do not guarantee WB for rat. |
|
| IHC-P | (6) |
1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
(Use at 1:1000 for rat species) Performed heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). |
| Notes |
|---|
|
IHC-Fr
1/500. Heat-mediated antigen retrieval using Tris-EDTA buffer (pH9.0) (ab94681) |
|
WB
1/1000. Predicted molecular weight: 53 kDa. Rat species is recommended based on IHC-P/ IHC-Fr results, we do not guarantee WB for rat. |
|
IHC-P
1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. (Use at 1:1000 for rat species) Performed heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). |
Target
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Function
This is a receptor for glucagon-like peptide 1. The activity of this receptor is mediated by G proteins which activate adenylyl cyclase. -
Sequence similarities
Belongs to the G-protein coupled receptor 2 family. -
Cellular localization
Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 14652 Mouse
- Entrez Gene: 25051 Rat
- SwissProt: O35659 Mouse
- SwissProt: P32301 Rat
- Unigene: 390969 Mouse
- Unigene: 11408 Rat
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Alternative names
- GLP 1 R antibody
- GLP 1 receptor antibody
- GLP 1R antibody
see all
Images
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Western blot - Anti-GLP-1R antibody [EPR21819] (ab218532)All lanes : Anti-GLP-1R antibody [EPR21819] (ab218532) at 1/1000 dilution
Lane 1 : Beta-TC-6 (Mouse pancreas insulinoma beta cell) whole cell lysate boiled. with NFDM/TBST
Lane 2 : Beta-TC-6 (Mouse pancreas insulinoma beta cell) whole cell lysate unboiled. with NFDM/TBST
Lysates/proteins at 20 µg per lane.
Blocking peptides at 5 % per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated.)
Predicted band size: 53 kDa
Observed band size: 46.66 kDa why is the actual band size different from the predicted?
Exposure time: 20 seconds -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GLP-1R antibody [EPR21819] (ab218532)](https://www.abcam.com/ps/products/218/ab218532/Images/ab218532-311715-anti-glp1r-antibody-epr21819-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GLP-1R antibody [EPR21819] (ab218532)Immunohistochemical analysis of paraffin-embedded rat pancreas tissue stained for GLP-1R using ab218532 at 1/1000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Cytoplasmic and membranous staining in rat pancreatic islet cells (PMID: 24467746) is observed. Counterstained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
Performed heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
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![Immunohistochemistry (Frozen sections) - Anti-GLP-1R antibody [EPR21819] (ab218532)](https://www.abcam.com/ps/products/218/ab218532/Images/ab218532-311713-anti-glp1r-antibody-epr21819-immunohistochemistry.jpg)
Immunohistochemistry (Frozen sections) - Anti-GLP-1R antibody [EPR21819] (ab218532)4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized rat pancreas tissue stained for GLP-1R (green) using ab218532 at 1/500 dilution, followed by AlexaFluor®488 Goat anti-Rabbit secondary antibody at 1/1000 dilution. Positive cytoplasmic and membranous staining in rat pancreatic islet cells (PMID: 18541709; 24695667) is observed. Nuclear counterstained with DAPI.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is AlexaFluor®488 Goat anti-Rabbit secondary antibody at 1/1000 dilution.
Heat-mediated antigen retrieval using Tris-EDTA buffer (pH9.0) (ab94681).
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GLP-1R antibody [EPR21819] (ab218532)](https://www.abcam.com/ps/products/218/ab218532/Images/ab218532-311714-anti-glp1r-antibody-epr21819-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GLP-1R antibody [EPR21819] (ab218532)Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue stained for GLP-1R using ab218532 at 1/2000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Positive staining of mouse pancreatic islet cells (PMID: 24467746) is observed. Counterstained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
Performed heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
-
![Immunohistochemistry (Frozen sections) - Anti-GLP-1R antibody [EPR21819] (ab218532)](https://www.abcam.com/ps/products/218/ab218532/Images/ab218532-311712-anti-glp1r-antibody-epr21819-immunohistochemistry.jpg)
Immunohistochemistry (Frozen sections) - Anti-GLP-1R antibody [EPR21819] (ab218532)4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized mouse pancreas tissue stained for GLP-1R (green) using ab218532 at 1/500 dilution, followed by AlexaFluor®488 Goat anti-Rabbit secondary antibody at 1/1000 dilution. Positive cytoplasmic and membranous staining in mouse pancreatic islet cells (PMID: 18541709; 24695667) is observed. Nuclear counterstained with DAPI.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is AlexaFluor®488 Goat anti-Rabbit secondary antibody at 1/1000 dilution.
Heat-mediated antigen retrieval using Tris-EDTA buffer (pH9.0) (ab94681).
-
![Anti-GLP-1R antibody [EPR21819] (ab218532)](https://www.abcam.com/ps/products/218/ab218532/Images/ab218532-6-benefits-of-recombinant-antibodies.png)
Anti-GLP-1R antibody [EPR21819] (ab218532)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (14)
ab218532 has been referenced in 14 publications.
- Kjær JN et al. Wellbeing, psychiatric morbidity and psychological distress amongst medical students in Denmark. Int J Soc Psychiatry N/A:207640221074916 (2022). PubMed: 35100882
- Pauza AG et al. GLP1R Attenuates Sympathetic Response to High Glucose via Carotid Body Inhibition. Circ Res 130:694-707 (2022). PubMed: 35100822
- Qian Z et al. Activation of glucagon-like peptide-1 receptor in microglia attenuates neuroinflammation-induced glial scarring via rescuing Arf and Rho GAP adapter protein 3 expressions after nerve injury. Int J Biol Sci 18:1328-1346 (2022). PubMed: 35280691
- Dai Q et al. miR-29c-5p knockdown reduces inflammation and blood-brain barrier disruption by upregulating LRP6. Open Med (Wars) 17:353-364 (2022). PubMed: 35799601
- Bjørnholm KD et al. Decreased expression of the GLP-1 receptor after segmental artery injury in mice. J Endocrinol 248:289-301 (2021). PubMed: 33449915