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    products/primary-antibodies/glucagon-antibody-ab137817.pdf

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Signal Transduction Growth Factors/Hormones Hormones
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Anti-Glucagon antibody (ab137817)

  • Datasheet
  • SDS
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Western blot - Anti-Glucagon antibody (ab137817)

    Key features and details

    • Rabbit polyclonal to Glucagon
    • Suitable for: WB
    • Reacts with: Human
    • Isotype: IgG

    Get better batch-to-batch reproducibility with a recombinant antibody

    Product image
    Anti-Glucagon antibody [EPR3070-45] (ab108426)
    • Research with confidence – consistent and reproducible results with every batch
    • Long-term and scalable supply – powered by recombinant technology for fast production
    • Success from the first experiment – confirmed specificity through extensive validation
    • Ethical standards compliant – production is animal-free

    Overview

    • Product name

      Anti-Glucagon antibody
      See all Glucagon primary antibodies
    • Description

      Rabbit polyclonal to Glucagon
    • Host species

      Rabbit
    • Tested applications

      Suitable for: WBmore details
    • Species reactivity

      Reacts with: Human
      Predicted to work with: Mouse, Rat, Cow, Dog
    • Immunogen

      Recombinant fragment, corresponding to a region within full lenght amino acids 1-180 of Human Glucagon

    • Positive control

      • A431, H1299, HeLa and Raji cell lysates
    • General notes

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
    • Storage buffer

      pH: 7.00
      Preservative: 0.01% Thimerosal (merthiolate)
      Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine)
    • Concentration information loading...
    • Purity

      Immunogen affinity purified
    • Clonality

      Polyclonal
    • Isotype

      IgG
    • Research areas

      • Signal Transduction
      • Growth Factors/Hormones
      • Hormones
      • Stem Cells
      • Lineage Markers
      • Endoderm
      • Cancer
      • Tumor biomarkers
      • Hormones
      • Developmental Biology
      • Lineage specification
      • Endoderm
      • Cancer
      • Cancer Metabolism
      • Metabolic signaling pathway
      • Integration of energy metabolism
      • Metabolism
      • Pathways and Processes
      • Metabolic signaling pathways
      • Energy transfer pathways
      • Integration of energy

    Associated products

    • Compatible Secondaries

      • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
      • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Isotype control

      • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
    • Positive Controls

      • HeLa whole cell lysate (ab29545)
      • Raji whole cell lysate (ab30124)
      • A-431 whole cell lysate (ab7909)
    • Recombinant Protein

      • Recombinant Human Glucagon protein (ab109948)

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab137817 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    WB
    1/500 - 1/3000. Predicted molecular weight: 21 kDa.
    Notes
    WB
    1/500 - 1/3000. Predicted molecular weight: 21 kDa.

    Target

    • Relevance

      Glucagon is a hormone that is secreted by alpha cells in the pancreas. Glucagon antagonizes insulin by converting glycogen to glucose in the liver and increasing blood sugar levels. Glucagon-like peptide 1 (GLP1), Glucagon-like peptide 2 (GLP2), VIP (vasoactive intestinal peptide) and PACAP (pituitary adenylate cyclase activating polypeptide) are in the glucagons hormone family. GLP1 is a transmitter in the central nervous system that regulates feeding and drinking behavior. GLP2 stimulates intestinal epithelial growth.
    • Cellular localization

      Secreted
    • Database links

      • Entrez Gene: 280802 Cow
      • Entrez Gene: 403571 Dog
      • Entrez Gene: 2641 Human
      • Entrez Gene: 14526 Mouse
      • Entrez Gene: 24952 Rat
      • Omim: 138030 Human
      • SwissProt: P01272 Cow
      • SwissProt: P01275 Human
      • SwissProt: P55095 Mouse
      • SwissProt: P06883 Rat
      see all
    • Alternative names

      • GCG antibody

    Images

    • Western blot - Anti-Glucagon antibody (ab137817)
      Western blot - Anti-Glucagon antibody (ab137817)
      Anti-Glucagon antibody (ab137817) at 1/500 dilution + Raji cell lysate at 30 µg

      Predicted band size: 21 kDa



      12% SDS PAGE

    Protocols

    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (0)

    Publishing research using ab137817? Please let us know so that we can cite the reference in this datasheet.

    ab137817 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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