Recombinant Anti-Glucagon antibody [EP3070] (ab92517)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucagon antibody [EP3070] (ab92517)](https://www.abcam.com/ps/products/92/ab92517/Images/ab92517-318538-anti-glucagon-antibody-ep3070-immunohistochemistry.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucagon antibody [EP3070] (ab92517)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP3070] to Glucagon
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-Glucagon antibody [EP3070]
See all Glucagon primary antibodies -
Description
Rabbit monoclonal [EP3070] to Glucagon -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide within Human Glucagon aa 50-150 (N terminal). The exact sequence is proprietary.
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Positive control
- IHC-P: Murine, human, and rat pancreatic tissue. WB: Capan-1 cell lysate, Mouse and rat pancreas tissue lysate
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.5% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EP3070 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab92517 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | (1) |
Use a concentration of 0.3 µg/ml. Predicted molecular weight: 21 kDa.
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| IHC-P | (4) |
1/8000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
See IHC antigen retrieval protocols. For unpurified use at 1/1000 - 1/5000. |
| Notes |
|---|
|
WB
Use a concentration of 0.3 µg/ml. Predicted molecular weight: 21 kDa. |
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IHC-P
1/8000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. See IHC antigen retrieval protocols. For unpurified use at 1/1000 - 1/5000. |
Target
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Relevance
Glucagon is a hormone that is secreted by alpha cells in the pancreas. Glucagon antagonizes insulin by converting glycogen to glucose in the liver and increasing blood sugar levels. Glucagon-like peptide 1 (GLP1), Glucagon-like peptide 2 (GLP2), VIP (vasoactive intestinal peptide) and PACAP (pituitary adenylate cyclase activating polypeptide) are in the glucagons hormone family. GLP1 is a transmitter in the central nervous system that regulates feeding and drinking behavior. GLP2 stimulates intestinal epithelial growth. -
Cellular localization
Secreted -
Database links
- Entrez Gene: 2641 Human
- Entrez Gene: 14526 Mouse
- Entrez Gene: 24952 Rat
- Omim: 138030 Human
- SwissProt: P01275 Human
- SwissProt: P55095 Mouse
- SwissProt: P06883 Rat
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Alternative names
- GCG antibody
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucagon antibody [EP3070] (ab92517)Image from Oelze M et al., PLoS One. 2014;9(11):e112394. Fig 1.; doi: 10.1371/journal.pone.0112394. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/
Glucagon measurements in controls and diabetic rats 8 weeks after STZ injection and 7 weeks of SGLT2i (low and high dose) treatment. Representative immunohistochemical stainings of pancreatic tissue.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucagon antibody [EP3070] (ab92517)](https://www.abcam.com/ps/products/92/ab92517/Images/ab92517-314300-anti-glucagon-antibody-ep3070-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucagon antibody [EP3070] (ab92517)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Rat pancreas tissue sections labeling Glucagon with Purified ab92517 at 1:8000 dilution (0.038 µg/ml). Heat mediated antigen retrieval was performed using using citrate (pH 6.0)Goat Anti-Rabbit IgG H&L (HRP) ab97051was used as the secondary antibody.Negative control:PBS instead of the primary antibody.Hematoxylinwas used as a counterstain -
![Western blot - Anti-Glucagon antibody [EP3070] (ab92517)](https://www.abcam.com/ps/products/92/ab92517/Images/ab92517-314258-anti-glucagon-antibody-ep3070-western-blot.jpg)
Western blot - Anti-Glucagon antibody [EP3070] (ab92517)Anti-Glucagon antibody [EP3070] (ab92517) at 0.3 µg/ml (purified) + Capan-1 (Human pancreas adenocarcinoma epithelial cell) whole cell lysates at 15 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 21 kDaBlocking and diluting buffer: 5% NFDM/TBST.
The bands around 75 kDa are non-specific signals.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucagon antibody [EP3070] (ab92517)](https://www.abcam.com/ps/products/92/ab92517/Images/ab92517-314299-anti-glucagon-antibody-ep3070-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucagon antibody [EP3070] (ab92517)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Mouse pancreas tissue sections labeling Glucagon with Purified ab92517 at 1:8000 dilution (0.038 µg/ml). Heat mediated antigen retrieval was performed using using citrate (pH 6.0)Goat Anti-Rabbit IgG H&L (HRP) ab97051was used as the secondary antibody.Negative control:PBS instead of the primary antibody.Hematoxylinwas used as a counterstain -
![Western blot - Anti-Glucagon antibody [EP3070] (ab92517)](https://www.abcam.com/ps/products/92/ab92517/Images/ab92517-314259-anti-glucagon-antibody-ep3070-western-blot.jpg)
Western blot - Anti-Glucagon antibody [EP3070] (ab92517)Anti-Glucagon antibody [EP3070] (ab92517) at 0.3 µg/ml (purified) + Mouse pancreas lysates at 20 µg
Secondary
Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Predicted band size: 21 kDa
Observed band size: 21 kDaBlocking and diluting buffer: 5% NFDM/TBST.
The bands around 75 kDa are non-specific signals.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucagon antibody [EP3070] (ab92517)](https://www.abcam.com/ps/products/92/ab92517/Images/ab92517-314298-anti-glucagon-antibody-ep3070-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucagon antibody [EP3070] (ab92517)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human pancreas tissue sections labeling Glucagon with Purified ab92517 at 1:8000 dilution (0.038 µg/ml). Heat mediated antigen retrieval was performed using using citrate (pH 6.0)Goat Anti-Rabbit IgG H&L (HRP) ab97051was used as the secondary antibody.Negative control:PBS instead of the primary antibody.Hematoxylinwas used as a counterstain -
![Western blot - Anti-Glucagon antibody [EP3070] (ab92517)](https://www.abcam.com/ps/products/92/ab92517/Images/ab92517-314260-anti-glucagon-antibody-ep3070-western-blot.jpg)
Western blot - Anti-Glucagon antibody [EP3070] (ab92517)Anti-Glucagon antibody [EP3070] (ab92517) at 0.3 µg/ml (purified) + Rat pancreas lysates at 15 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 21 kDaBlocking and diluting buffer: 5% NFDM/TBST.
The bands around 75 kDa are non-specific signals.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucagon antibody [EP3070] (ab92517)](https://www.abcam.com/ps/products/92/ab92517/Images/ab92517-254192-anti-glucagon-antibody-ep3070-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucagon antibody [EP3070] (ab92517)Image is courtesy of an anonymous AbReview.Immunohistochemical analysis of PFA-fixed paraffin-embedded murine pancreatic tissue sections, labelling glucagon with unpurified ab92517 at a dilution of 1/2500 incubated for 18 hours at 4°C. Antigen retrival was performed through heat-mediated 10mM sodium citrate pH 6.0 incubation. Blocking was done with 5% serum for 1 hour at 25°C. The secondary was a donkey anti-rabbit polyclonal Alexa Fluor® 488 conjugate at 1/400. Counterstaining is with DAPI (blue) against nuclear DNA.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucagon antibody [EP3070] (ab92517)](https://www.abcam.com/ps/products/92/ab92517/Images/ab92517-107619-anti-glucagon-antibody-ep3070-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucagon antibody [EP3070] (ab92517)Unpurified ab92517, at 1/1000 dilution, staining Glucagon in paraffin embedded Human pancreas tissue by Immunohistochemistry.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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![Anti-Glucagon antibody [EP3070] (ab92517)](https://www.abcam.com/ps/products/92/ab92517/Images/ab92517-14-benefits-of-recombinant-antibodies.png)
Anti-Glucagon antibody [EP3070] (ab92517)
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (63)
ab92517 has been referenced in 63 publications.
- Benderradji H et al. Impaired Glucose Homeostasis in a Tau Knock-In Mouse Model. Front Mol Neurosci 15:841892 (2022). PubMed: 35250480
- Lei L et al. Morus alba L. (Sangzhi) Alkaloids Promote Insulin Secretion, Restore Diabetic β-Cell Function by Preventing Dedifferentiation and Apoptosis. Front Pharmacol 13:841981 (2022). PubMed: 35308210
- Cao H et al. Autotaxin signaling facilitates β cell dedifferentiation and dysfunction induced by Sirtuin 3 deficiency. Mol Metab 60:101493 (2022). PubMed: 35398277
- Wang MY et al. Glucagon blockade restores functional ß-cell mass in type 1 diabetic mice and enhances function of human islets. Proc Natl Acad Sci U S A 118:N/A (2021). PubMed: 33619103
- Kim GS et al. Optimal allogeneic islet dose for transplantation in insulin-dependent diabetic Macaca fascicularis monkeys. Sci Rep 11:8617 (2021). PubMed: 33883656