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RecombinantRabMAb

Recombinant Anti-Glucagon antibody [EP3070] - BSA and Azide free (ab167078)

Submit a review Submit a question References (1)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucagon antibody [EP3070] - BSA and Azide free (ab167078)
  • Western blot - Anti-Glucagon antibody [EP3070] - BSA and Azide free (ab167078)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucagon antibody [EP3070] - BSA and Azide free (ab167078)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucagon antibody [EP3070] - BSA and Azide free (ab167078)
  • Western blot - Anti-Glucagon antibody [EP3070] - BSA and Azide free (ab167078)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucagon antibody [EP3070] - BSA and Azide free (ab167078)
  • Anti-Glucagon antibody [EP3070] - BSA and Azide free (ab167078)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP3070] to Glucagon - BSA and Azide free
  • Suitable for: WB, IHC-P
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Alexa Fluor® 568 Unconjugated

Overview

  • Product name

    Anti-Glucagon antibody [EP3070] - BSA and Azide free
    See all Glucagon primary antibodies

  • Description

    Rabbit monoclonal [EP3070] to Glucagon - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-Pmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide within Human Glucagon aa 50-150 (N terminal). The exact sequence is proprietary.
    Database link: P01275

  • Positive control

    • IHC-P: Mouse, rat and human pancreas tissue. WB: Capan-1 cells and mouse and rat tissue lysates.

  • General notes

    ab167078 is the carrier-free version of ab92517.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab167078 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
Use at an assay dependent concentration. Predicted molecular weight: 21 kDa.

Please refer to the original abID, ab92517, for information on recommended dilutions.
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Please refer to the original abID, ab92517, for information on recommended dilutions.
Notes
WB
Use at an assay dependent concentration. Predicted molecular weight: 21 kDa.

Please refer to the original abID, ab92517, for information on recommended dilutions.
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Please refer to the original abID, ab92517, for information on recommended dilutions.

Target

  • Relevance

    Glucagon is a hormone that is secreted by alpha cells in the pancreas. Glucagon antagonizes insulin by converting glycogen to glucose in the liver and increasing blood sugar levels. Glucagon-like peptide 1 (GLP1), Glucagon-like peptide 2 (GLP2), VIP (vasoactive intestinal peptide) and PACAP (pituitary adenylate cyclase activating polypeptide) are in the glucagons hormone family. GLP1 is a transmitter in the central nervous system that regulates feeding and drinking behavior. GLP2 stimulates intestinal epithelial growth.

  • Cellular localization

    Secreted

  • Database links

    • Alternative names

      • GCG antibody

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucagon antibody [EP3070] - BSA and Azide free (ab167078)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucagon antibody [EP3070] - BSA and Azide free (ab167078)

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human pancreas tissue sections labeling Glucagon with purified ab92517 at 1/8000 dilution (0.038 µg/ml). Heat mediated antigen retrieval was performed using using citrate (pH 6.0). Goat Anti-Rabbit IgG H&L (HRP) ab97051 was used as the secondary antibody.

      Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab92517).

    • Western blot - Anti-Glucagon antibody [EP3070] - BSA and Azide free (ab167078)
      Western blot - Anti-Glucagon antibody [EP3070] - BSA and Azide free (ab167078)
      Anti-Glucagon antibody [EP3070] - BSA and Azide free (ab167078) at 1/1000 dilution + Capan-1 ( Human pancreas adenocarcinoma epithelial cell) whole cell lysate at 15 µg

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 21 kDa



      The bands around 75 kDa are non-specific signals.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucagon antibody [EP3070] - BSA and Azide free (ab167078)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucagon antibody [EP3070] - BSA and Azide free (ab167078)

      Unpurified ab92517, at 1/1000 dilution, staining Glucagon in paraffin embedded human pancreas tissue by Immunohistochemistry.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab92517).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucagon antibody [EP3070] - BSA and Azide free (ab167078)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucagon antibody [EP3070] - BSA and Azide free (ab167078)

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse pancreas tissue sections labeling Glucagon with purified ab92517 at 1/8000 dilution (0.038 µg/ml). Heat mediated antigen retrieval was performed using using citrate (pH 6.0). Goat Anti-Rabbit IgG H&L (HRP) ab97051 was used as the secondary antibody.

      Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab92517).

    • Western blot - Anti-Glucagon antibody [EP3070] - BSA and Azide free (ab167078)
      Western blot - Anti-Glucagon antibody [EP3070] - BSA and Azide free (ab167078)
      All lanes : Anti-Glucagon antibody [EP3070] - BSA and Azide free (ab167078) at 1/5000 dilution

      Lane 1 : Mouse pancreas lysates
      Lane 2 : Rat pancreas lysates

      Lysates/proteins at 15 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 21 kDa



      The bands around 75 kDa are non-specific signals.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucagon antibody [EP3070] - BSA and Azide free (ab167078)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucagon antibody [EP3070] - BSA and Azide free (ab167078)

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat pancreas tissue sections labeling Glucagon with purified ab92517 at 1/8000 dilution (0.038 µg/ml). Heat mediated antigen retrieval was performed using using citrate (pH 6.0). Goat Anti-Rabbit IgG H&L (HRP) ab97051 was used as the secondary antibody.

      Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab92517).

    • Anti-Glucagon antibody [EP3070] - BSA and Azide free (ab167078)
      Anti-Glucagon antibody [EP3070] - BSA and Azide free (ab167078)

    References (1)

    Publishing research using ab167078? Please let us know so that we can cite the reference in this datasheet.

    ab167078 has been referenced in 1 publication.

    • Essaouiba A  et al. Microwell-based pancreas-on-chip model enhances genes expression and functionality of rat islets of Langerhans. Mol Cell Endocrinol N/A:110892 (2020). PubMed: 32531418

    Customer reviews and Q&As

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