Recombinant Anti-Glucagon antibody [EP3070] - BSA and Azide free (ab167078)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP3070] to Glucagon - BSA and Azide free
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-Glucagon antibody [EP3070] - BSA and Azide free
See all Glucagon primary antibodies -
Description
Rabbit monoclonal [EP3070] to Glucagon - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide within Human Glucagon aa 50-150 (N terminal). The exact sequence is proprietary.
Database link: P01275 -
Positive control
- IHC-P: Mouse, rat and human pancreas tissue. WB: Capan-1 cells and mouse and rat tissue lysates.
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General notes
ab167078 is the carrier-free version of ab92517.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
Constituent: PBS -
Carrier free
Yes -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EP3070 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
- Alexa Fluor® 647 Anti-Glucagon antibody [EP3070] (ab307337)
- Alexa Fluor® 488 Anti-Glucagon antibody [EP3070] (ab307340)
- Alexa Fluor® 594 Anti-Glucagon antibody [EP3070] (ab311840)
- Alexa Fluor® 568 Anti-Glucagon antibody [EP3070] (ab313123)
- Alexa Fluor® 555 Anti-Glucagon antibody [EP3070] (ab313316)
- Anti-Glucagon antibody [EP3070] (ab92517)
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab167078 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use at an assay dependent concentration. Predicted molecular weight: 21 kDa.
Please refer to the original abID, ab92517, for information on recommended dilutions. |
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IHC-P |
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Please refer to the original abID, ab92517, for information on recommended dilutions. |
Notes |
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WB
Use at an assay dependent concentration. Predicted molecular weight: 21 kDa. Please refer to the original abID, ab92517, for information on recommended dilutions. |
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Please refer to the original abID, ab92517, for information on recommended dilutions. |
Target
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Relevance
Glucagon is a hormone that is secreted by alpha cells in the pancreas. Glucagon antagonizes insulin by converting glycogen to glucose in the liver and increasing blood sugar levels. Glucagon-like peptide 1 (GLP1), Glucagon-like peptide 2 (GLP2), VIP (vasoactive intestinal peptide) and PACAP (pituitary adenylate cyclase activating polypeptide) are in the glucagons hormone family. GLP1 is a transmitter in the central nervous system that regulates feeding and drinking behavior. GLP2 stimulates intestinal epithelial growth. -
Cellular localization
Secreted -
Database links
- Entrez Gene: 2641 Human
- Entrez Gene: 14526 Mouse
- Entrez Gene: 24952 Rat
- Omim: 138030 Human
- SwissProt: P01275 Human
- SwissProt: P55095 Mouse
- SwissProt: P06883 Rat
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Alternative names
- GCG antibody
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucagon antibody [EP3070] - BSA and Azide free (ab167078)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human pancreas tissue sections labeling Glucagon with purified ab92517 at 1/8000 dilution (0.038 µg/ml). Heat mediated antigen retrieval was performed using using citrate (pH 6.0). Goat Anti-Rabbit IgG H&L (HRP) ab97051 was used as the secondary antibody.
Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab92517).
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Anti-Glucagon antibody [EP3070] - BSA and Azide free (ab167078) at 1/1000 dilution + Capan-1 ( Human pancreas adenocarcinoma epithelial cell) whole cell lysate at 15 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 21 kDaThe bands around 75 kDa are non-specific signals.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucagon antibody [EP3070] - BSA and Azide free (ab167078)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse pancreas tissue sections labeling Glucagon with purified ab92517 at 1/8000 dilution (0.038 µg/ml). Heat mediated antigen retrieval was performed using using citrate (pH 6.0). Goat Anti-Rabbit IgG H&L (HRP) ab97051 was used as the secondary antibody.
Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab92517).
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All lanes : Anti-Glucagon antibody [EP3070] - BSA and Azide free (ab167078) at 1/5000 dilution
Lane 1 : Mouse pancreas lysates
Lane 2 : Rat pancreas lysates
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 21 kDaThe bands around 75 kDa are non-specific signals.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucagon antibody [EP3070] - BSA and Azide free (ab167078)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat pancreas tissue sections labeling Glucagon with purified ab92517 at 1/8000 dilution (0.038 µg/ml). Heat mediated antigen retrieval was performed using using citrate (pH 6.0). Goat Anti-Rabbit IgG H&L (HRP) ab97051 was used as the secondary antibody.
Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab92517).
Protocols
Datasheets and documents
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Datasheet download
References (1)
ab167078 has been referenced in 1 publication.
- Essaouiba A et al. Microwell-based pancreas-on-chip model enhances genes expression and functionality of rat islets of Langerhans. Mol Cell Endocrinol N/A:110892 (2020). PubMed: 32531418