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RecombinantRabMAb

Recombinant Anti-Glucagon antibody [EPR3070-45] - BSA and Azide free (ab226035)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucagon antibody [EPR3070-45] - BSA and Azide free (ab226035)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucagon antibody [EPR3070-45] - BSA and Azide free (ab226035)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucagon antibody [EPR3070-45] - BSA and Azide free (ab226035)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucagon antibody [EPR3070-45] - BSA and Azide free (ab226035)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucagon antibody [EPR3070-45] - BSA and Azide free (ab226035)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucagon antibody [EPR3070-45] - BSA and Azide free (ab226035)
  • OI-RD Scanning - Anti-Glucagon antibody [EPR3070-45] - BSA and Azide free (ab226035)
  • Anti-Glucagon antibody [EPR3070-45] - BSA and Azide free (ab226035)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR3070-45] to Glucagon - BSA and Azide free
  • Suitable for: IHC-P, WB
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Unconjugated

Overview

  • Product name

    Anti-Glucagon antibody [EPR3070-45] - BSA and Azide free
    See all Glucagon primary antibodies

  • Description

    Rabbit monoclonal [EPR3070-45] to Glucagon - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-P, WBmore details
    Unsuitable for: Flow Cyt or ICC/IF

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IHC-P: Human pancreas tissue; skeletal muscle tissue; lung tissue; kidney tissue; brain tissue.

  • General notes

    ab226035 is the carrier-free version of ab108426.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab226035 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Antigen retrieval is recommended.
WB
Use at an assay dependent concentration. Predicted molecular weight: 21 kDa.
Notes
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Antigen retrieval is recommended.
WB
Use at an assay dependent concentration. Predicted molecular weight: 21 kDa.
Application notes
Is unsuitable for Flow Cyt or ICC/IF.

Target

  • Relevance

    Glucagon is a hormone that is secreted by alpha cells in the pancreas. Glucagon antagonizes insulin by converting glycogen to glucose in the liver and increasing blood sugar levels. Glucagon-like peptide 1 (GLP1), Glucagon-like peptide 2 (GLP2), VIP (vasoactive intestinal peptide) and PACAP (pituitary adenylate cyclase activating polypeptide) are in the glucagons hormone family. GLP1 is a transmitter in the central nervous system that regulates feeding and drinking behavior. GLP2 stimulates intestinal epithelial growth.

  • Cellular localization

    Secreted

  • Database links

    • Alternative names

      • GCG antibody

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucagon antibody [EPR3070-45] - BSA and Azide free (ab226035)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucagon antibody [EPR3070-45] - BSA and Azide free (ab226035)

      This IHC data was generated using the same anti-Glucagon antibody clone, EPR3070-45, in a different buffer formulation (cat# ab108426).

      Immunohistochemical staining of paraffin embedded human pancreas with purified ab108426 at a working dilution of 1/250. The secondary antibody used is HRP goat anti-rabbit IgG H&L (ab97051) at 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucagon antibody [EPR3070-45] - BSA and Azide free (ab226035)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucagon antibody [EPR3070-45] - BSA and Azide free (ab226035)

      This IHC data was generated using the same anti-Glucagon antibody clone, EPR3070-45, in a different buffer formulation (cat# ab108426).

      Unpurified ab108426, at 1/100 dilution, staining Glucagon in paraffin-embedded Human pancreas tissue by Immunohistochemistry.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucagon antibody [EPR3070-45] - BSA and Azide free (ab226035)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucagon antibody [EPR3070-45] - BSA and Azide free (ab226035)

      Unpurified ab108426 showing negative staining in Skeletal muscle tissue.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab108426).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucagon antibody [EPR3070-45] - BSA and Azide free (ab226035)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucagon antibody [EPR3070-45] - BSA and Azide free (ab226035)

      Unpurified ab108426 showing negative staining in Normal lung tissue.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab108426).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucagon antibody [EPR3070-45] - BSA and Azide free (ab226035)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucagon antibody [EPR3070-45] - BSA and Azide free (ab226035)

      Unpurified ab108426 showing negative staining in Normal kidney tissue.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab108426).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucagon antibody [EPR3070-45] - BSA and Azide free (ab226035)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucagon antibody [EPR3070-45] - BSA and Azide free (ab226035)

      Unpurified ab108426 showing negative staining in Normal brain tissue.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab108426).

    • OI-RD Scanning - Anti-Glucagon antibody [EPR3070-45] - BSA and Azide free (ab226035)
      OI-RD Scanning - Anti-Glucagon antibody [EPR3070-45] - BSA and Azide free (ab226035)
      Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab108426).

    • Anti-Glucagon antibody [EPR3070-45] - BSA and Azide free (ab226035)
      Anti-Glucagon antibody [EPR3070-45] - BSA and Azide free (ab226035)

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (0)

    Publishing research using ab226035? Please let us know so that we can cite the reference in this datasheet.

    ab226035 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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