Anti-Glucagon antibody [K79bB10] (ab10988)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucagon antibody [K79bB10] (ab10988)](https://www.abcam.com/ps/products/10/ab10988/Images/ab10988-394088-anti-glucagon-antibody-k79bb10-immunohistochemistry-pancreas-rat.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucagon antibody [K79bB10] (ab10988)")
Key features and details
- Mouse monoclonal [K79bB10] to Glucagon
- Suitable for: IHC-P
- Reacts with: Rat
- Isotype: IgG1
Overview
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Product name
Anti-Glucagon antibody [K79bB10]
See all Glucagon primary antibodies -
Description
Mouse monoclonal [K79bB10] to Glucagon -
Host species
Mouse -
Tested applications
Suitable for: IHC-Pmore details -
Species reactivity
Reacts with: Rat
Predicted to work with: Rabbit, Guinea pig, Cat, Dog, Human, Pig, Common marmoset, Syrian hamster
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Immunogen
Full length protein corresponding to Pig Glucagon. Polymerized porcine glucagon.
Database link: P01275 -
Positive control
- Pancreatic glucagon for RIA. IHC-P: Mouse and Rat pancreas tissues.
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General notes
Production of this antibody has been changed on 8th July 2016. The following lots are from ascites and are still in stock as of 8th July 2016: GR260160 and GR268340. Lot numbers higher than GR268340 will be purified from tissue culture supernatant. Please note that the dilutions may need to be adjusted accordingly.
If slight turbidity occurs upon prolonged storage, clarify by centrifugation before use.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.40
Preservative: 0.097% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Proprietary Purification -
Purification notes
Purified from Tissue culture supernatant. -
Clonality
Monoclonal -
Clone number
K79bB10 -
Isotype
IgG1 -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab10988 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | (10) |
Use at an assay dependent concentration.
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| Notes |
|---|
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IHC-P
Use at an assay dependent concentration. |
Target
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Relevance
Glucagon is a hormone that is secreted by alpha cells in the pancreas. Glucagon antagonizes insulin by converting glycogen to glucose in the liver and increasing blood sugar levels. Glucagon-like peptide 1 (GLP1), Glucagon-like peptide 2 (GLP2), VIP (vasoactive intestinal peptide) and PACAP (pituitary adenylate cyclase activating polypeptide) are in the glucagons hormone family. GLP1 is a transmitter in the central nervous system that regulates feeding and drinking behavior. GLP2 stimulates intestinal epithelial growth. -
Cellular localization
Secreted -
Database links
- Entrez Gene: 403571 Dog
- Entrez Gene: 2641 Human
- Entrez Gene: 397595 Pig
- Entrez Gene: 24952 Rat
- Omim: 138030 Human
- SwissProt: P01275 Human
- SwissProt: P01274 Pig
- SwissProt: P68274 Rabbit
see all -
Alternative names
- GCG antibody
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucagon antibody [K79bB10] (ab10988)
Formali-fixed, paraffin-embedded rat pancreas tissue stained for Glucagon using ab10988 at 10 µg/ml in immunohistochemical analysis.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucagon antibody [K79bB10] (ab10988)](https://www.abcam.com/ps/products/10/ab10988/Images/ab10988-18695-anti-glucagon-antibody-k79bb10-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucagon antibody [K79bB10] (ab10988)Image courtesy of Thomas Schoeppe by Abreview.ab10988 staining Glucagon in rat pancreatic tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).
Tissue was fixed in paraformaldehyde, blocked with 2% serum for 1 hour at 25°C, then incubated with ab10988 at a 1/200 dilution for 1 hour at 25°C. The secondary used was a donkey anti-mouse IgG (H+L) conjugated to Texas Red, used at a 1/250 dilution. Nuclei counterstained with DAPI, green is staining for insulin.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (140)
ab10988 has been referenced in 140 publications.
- Han MS et al. A feed-forward regulatory loop in adipose tissue promotes signaling by the hepatokine FGF21. Genes Dev 35:133-146 (2021). PubMed: 33334822
- Ni Q et al. Proper mTORC1 Activity Is Required for Glucose Sensing and Early Adaptation in Human Pancreatic ß Cells. J Clin Endocrinol Metab 106:e562-e572 (2021). PubMed: 33120423
- Werneck-de-Castro JP et al. The RNA-binding protein LARP1 is dispensable for pancreatic ß-cell function and mass. Sci Rep 11:2079 (2021). PubMed: 33483593
- Soltani A et al. Treatment of diabetic mice by microfluidic system-assisted transplantation of stem cells-derived insulin-producing cells transduced with miRNA. Life Sci 274:119338 (2021). PubMed: 33716064
- Ramirez-Hernandez G et al. Global Deletion of the Prolactin Receptor Aggravates Streptozotocin-Induced Diabetes in Mice. Front Endocrinol (Lausanne) 12:619696 (2021). PubMed: 33746901