Anti-Glucagon antibody - N-terminal (ab189279)
Key features and details
- Rabbit polyclonal to Glucagon - N-terminal
- Suitable for: IHC-P, WB, Flow Cyt (Intra)
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
-
Product name
Anti-Glucagon antibody - N-terminal
See all Glucagon primary antibodies -
Description
Rabbit polyclonal to Glucagon - N-terminal -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WB, Flow Cyt (Intra)more details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Synthetic peptide within Human Glucagon aa 1-100 (N terminal) conjugated to keyhole limpet haemocyanin. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: P01275 -
Positive control
- IHC-P: Human pancreas, islets of Langerhans; Human hepatocarcinoma tissue. Flow Cyt (Intra): HepG2 cells. WB: Mouse bladder tissue lysate.
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.4
Preservative: 0.09% Sodium azide
Constituent: 99% PBS -
Concentration information loading... -
Purity
Ammonium Sulphate Precipitation -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
-
Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab189279 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P |
Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
|
|
| WB |
1/1000. Predicted molecular weight: 21 kDa.
|
|
| Flow Cyt (Intra) |
1/10 - 1/50.
ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody. |
| Notes |
|---|
|
IHC-P
Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
|
WB
1/1000. Predicted molecular weight: 21 kDa. |
|
Flow Cyt (Intra)
1/10 - 1/50. ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody. |
Target
-
Relevance
Glucagon is a hormone that is secreted by alpha cells in the pancreas. Glucagon antagonizes insulin by converting glycogen to glucose in the liver and increasing blood sugar levels. Glucagon-like peptide 1 (GLP1), Glucagon-like peptide 2 (GLP2), VIP (vasoactive intestinal peptide) and PACAP (pituitary adenylate cyclase activating polypeptide) are in the glucagons hormone family. GLP1 is a transmitter in the central nervous system that regulates feeding and drinking behavior. GLP2 stimulates intestinal epithelial growth. -
Cellular localization
Secreted -
Database links
- Entrez Gene: 2641 Human
- Entrez Gene: 14526 Mouse
- Omim: 138030 Human
- SwissProt: P01275 Human
- SwissProt: P55095 Mouse
-
Alternative names
- GCG antibody
Images
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucagon antibody - N-terminal (ab189279)
Immunohistochemical analysis of formalin-fixed, paraffin-embedded human pancreas, islets of Langerhans labeling Glucagon with ab189279 at 10 µg/ml.
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucagon antibody - N-terminal (ab189279)Immunohistochemical analysis of formalin-fixed, paraffin-embedded human hepatocarcinoma tissue labeling Glucagon with ab189279 at 10 µg/ml, followed by peroxidase-conjugated secondary antibody and DAB staining.
-
Flow Cytometry (Intracellular) - Anti-Glucagon antibody - N-terminal (ab189279)Intracellular flow cytometric analysis of HepG2 cells labeling Glucagon with ab189279 at 1/10 dilution (bottom histogram) compared to negative control cells (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
-
Western blot - Anti-Glucagon antibody - N-terminal (ab189279)Anti-Glucagon antibody - N-terminal (ab189279) at 1/1000 dilution + Mouse bladder tissue lysate at 35 µg
Predicted band size: 21 kDa
Protocols
Datasheets and documents
-
Datasheet download
References (1)
ab189279 has been referenced in 1 publication.
- Zhang S et al. Porcine pancreas mesenchymal cell characterization and functional differentiation into insulin-producing cells in vitro. Mol Med Rep 24:N/A (2021). PubMed: 34414446
