Recombinant Anti-Glutamate Receptor 1 (AMPA subtype) antibody [EPR19522] (ab183797)
![Western blot - Anti-Glutamate Receptor 1 (AMPA subtype) antibody [EPR19522] (ab183797)](https://www.abcam.com/ps/products/183/ab183797/Images/ab183797-263072-anti-glutamate-receptor-1-ampa-subtype-antibody-epr19522-western-blot.jpg "Western blot - Anti-Glutamate Receptor 1 (AMPA subtype) antibody [EPR19522] (ab183797)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR19522] to Glutamate Receptor 1 (AMPA subtype)
- Suitable for: WB, IHC-P, IP, IHC-Fr
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-Glutamate Receptor 1 (AMPA subtype) antibody [EPR19522]
See all Glutamate Receptor 1 (AMPA subtype) primary antibodies -
Description
Rabbit monoclonal [EPR19522] to Glutamate Receptor 1 (AMPA subtype) -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, IP, IHC-Frmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Mouse Glutamate Receptor 1 fragment recombinant protein; Mouse brain, hippocampus and cerebellum lysates; Rat brain, cerebellum and hippocampus lysates; Human cerebellum and fetal brain lysates. IHC-P: Mouse and rat hippocampus tissues. IHC-Fr: Mouse hippocampus tissue. IP: Mouse brain whole cell lysate.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR19522 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab183797 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/1000. Detects a band of approximately 102 kDa (predicted molecular weight: 102 kDa).
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| IHC-P |
1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
IHC is recommended for mouse and rat only. |
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| IP |
1/30.
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| IHC-Fr |
1/100.
Antigen retrieval: Heated citrate solution (10mM citrate PH 6.0 + 0.05% Tween-20) IHC is recommended for mouse and rat only |
| Notes |
|---|
|
WB
1/1000. Detects a band of approximately 102 kDa (predicted molecular weight: 102 kDa). |
|
IHC-P
1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. IHC is recommended for mouse and rat only. |
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IP
1/30. |
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IHC-Fr
1/100. Antigen retrieval: Heated citrate solution (10mM citrate PH 6.0 + 0.05% Tween-20) IHC is recommended for mouse and rat only |
Target
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Function
Ionotropic glutamate receptor. L-glutamate acts as an excitatory neurotransmitter at many synapses in the central nervous system. Binding of the excitatory neurotransmitter L-glutamate induces a conformation change, leading to the opening of the cation channel, and thereby converts the chemical signal to an electrical impulse. The receptor then desensitizes rapidly and enters a transient inactive state, characterized by the presence of bound agonist. -
Tissue specificity
Widely expressed in brain. -
Sequence similarities
Belongs to the glutamate-gated ion channel (TC 1.A.10.1) family. GRIA1 subfamily. -
Post-translational
modificationsPalmitoylated. Depalmitoylated upon glutamate stimulation. Cys-603 palmitoylation leads to Golgi retention and decreased cell surface expression. In contrast, Cys-829 palmitoylation does not affect cell surface expression but regulates stimulation-dependent endocytosis. -
Cellular localization
Cell membrane. Endoplasmic reticulum membrane. Cell junction > synapse > postsynaptic cell membrane. Interaction with CACNG2 promotes cell surface expression. - Information by UniProt
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Database links
- Entrez Gene: 2890 Human
- Entrez Gene: 14799 Mouse
- Entrez Gene: 50592 Rat
- Omim: 138248 Human
- SwissProt: P42261 Human
- SwissProt: P23818 Mouse
- SwissProt: P19490 Rat
- Unigene: 519693 Human
see all -
Alternative names
- GLUR 1 antibody
- GLUR A antibody
- AMPA 1 antibody
see all
Images
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Western blot - Anti-Glutamate Receptor 1 (AMPA subtype) antibody [EPR19522] (ab183797)All lanes : Anti-Glutamate Receptor 1 (AMPA subtype) antibody [EPR19522] (ab183797) at 1/5000 dilution
Lane 1 : Mouse Glutamate Receptor 1 fragment recombinant protein
Lane 2 : Mouse Glutamate Receptor 2 fragment recombinant protein
Lysates/proteins at 0.01 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 102 kDaBlocking/Dilution buffer: 5% NFDM/TBST.
Exposure times: Lane 1: 2 seconds; Lane 2: 3 minutes.
Mouse Glutamate Receptor 1 fragment recombinant protein contains aa19-184 with a GST/His-Tag®. Mouse Glutamate Receptor 2 fragment recombinant protein contains aa25-288 with a His-Tag®.
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![Western blot - Anti-Glutamate Receptor 1 (AMPA subtype) antibody [EPR19522] (ab183797)](https://www.abcam.com/ps/products/183/ab183797/Images/ab183797-263071-anti-glutamate-receptor-1-ampa-subtype-antibody-epr19522-western-blot.jpg)
Western blot - Anti-Glutamate Receptor 1 (AMPA subtype) antibody [EPR19522] (ab183797)All lanes : Anti-Glutamate Receptor 1 (AMPA subtype) antibody [EPR19522] (ab183797) at 1/1000 dilution
Lane 1 : Mouse brain lysate
Lane 2 : Rat liver lysate
Lane 3 : Mouse hippocampus lysate
Lane 4 : Mouse cerebellum lysate
Lane 5 : Rat brain lysate
Lane 6 : Rat cerebellum lysate
Lane 7 : Rat hippocampus lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 102 kDa
Observed band size: 102 kDaBlocking/Dilution buffer: 5% NFDM/TBST.
Exposure times: Lane 1 and 2: 30 seconds; Lane 3, 4, 5, 6 and 7: 15 seconds.
Rodent Glutamate Receptor 1 is widely expressed in brain and represents the predominant excitatory neurotransmitter system but not in liver.
Negative control: Rat liver (PMID: 2480522).
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![Western blot - Anti-Glutamate Receptor 1 (AMPA subtype) antibody [EPR19522] (ab183797)](https://www.abcam.com/ps/products/183/ab183797/Images/ab183797-263070-anti-glutamate-receptor-1-ampa-subtype-antibody-epr19522-western-blot.jpg)
Western blot - Anti-Glutamate Receptor 1 (AMPA subtype) antibody [EPR19522] (ab183797)All lanes : Anti-Glutamate Receptor 1 (AMPA subtype) antibody [EPR19522] (ab183797) at 1/1000 dilution
Lane 1 : Human cerebellum lysate
Lane 2 : Human muscle lysate
Lane 3 : Human fetal brain lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution
Predicted band size: 102 kDa
Observed band size: 102 kDa
Exposure time: 3 minutesBlocking/Dilution buffer: 5% NFDM/TBST.
Human Glutamate Receptor 1 is widely expressed in brain and represents the predominant excitatory neurotransmitter system but not in muscle.
Negative control: human muscle (PMID: 1652753).
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glutamate Receptor 1 (AMPA subtype) antibody [EPR19522] (ab183797)](https://www.abcam.com/ps/products/183/ab183797/Images/ab183797-263069-anti-glutamate-receptor-1-ampa-subtype-antibody-epr19522-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glutamate Receptor 1 (AMPA subtype) antibody [EPR19522] (ab183797)Immunohistochemical analysis of paraffin-embedded Mouse hippocampus tissue labeling Glutamate Receptor 1 (AMPA subtype) with ab183797 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasm staining on mouse hippocampus was observed [PMID: 15723058]. Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab97051 at 1/500 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glutamate Receptor 1 (AMPA subtype) antibody [EPR19522] (ab183797)](https://www.abcam.com/ps/products/183/ab183797/Images/ab183797-263067-anti-glutamate-receptor-1-ampa-subtype-antibody-epr19522-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glutamate Receptor 1 (AMPA subtype) antibody [EPR19522] (ab183797)Immunohistochemical analysis of paraffin-embedded Mouse liver tissue labeling Glutamate Receptor 1 (AMPA subtype) with ab183797 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. No staining on mouse liver is observed. Counter stained with Hematoxylin.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glutamate Receptor 1 (AMPA subtype) antibody [EPR19522] (ab183797)](https://www.abcam.com/ps/products/183/ab183797/Images/ab183797-263068-anti-glutamate-receptor-1-ampa-subtype-antibody-epr19522-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glutamate Receptor 1 (AMPA subtype) antibody [EPR19522] (ab183797)Immunohistochemical analysis of paraffin-embedded Rat hippocampus tissue labeling Glutamate Receptor 1 (AMPA subtype) with ab183797 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasm staining on rat hippocampus is observed [PMID: 15723058]. Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab97051 at 1/500 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glutamate Receptor 1 (AMPA subtype) antibody [EPR19522] (ab183797)](https://www.abcam.com/ps/products/183/ab183797/Images/ab183797-263066-anti-glutamate-receptor-1-ampa-subtype-antibody-epr19522-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glutamate Receptor 1 (AMPA subtype) antibody [EPR19522] (ab183797)Immunohistochemical analysis of paraffin-embedded Rat stomach tissue labeling Glutamate Receptor 1 (AMPA subtype) with ab183797 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. No staining on rat stomach is observed. Counter stained with Hematoxylin.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
-
![Immunohistochemistry (Frozen sections) - Anti-Glutamate Receptor 1 (AMPA subtype) antibody [EPR19522] (ab183797)](https://www.abcam.com/ps/products/183/ab183797/Images/ab183797-263065-anti-glutamate-receptor-1-ampa-subtype-antibody-epr19522-immunohistochemistry.jpg)
Immunohistochemistry (Frozen sections) - Anti-Glutamate Receptor 1 (AMPA subtype) antibody [EPR19522] (ab183797)Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized frozen Mouse hippocampus tissue labeling Glutamate Receptor 1 (AMPA subtype) with ab183797 at 1/100 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Cytoplasm staining on mouse hippocampus was observed. The nuclear counterstain is DAPI (blue).
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab150077 secondary antibody at 1/1000 dilution.
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![Immunohistochemistry (Frozen sections) - Anti-Glutamate Receptor 1 (AMPA subtype) antibody [EPR19522] (ab183797)](https://www.abcam.com/ps/products/183/ab183797/Images/ab183797-263064-anti-glutamate-receptor-1-ampa-subtype-antibody-epr19522-immunohistochemistry.jpg)
Immunohistochemistry (Frozen sections) - Anti-Glutamate Receptor 1 (AMPA subtype) antibody [EPR19522] (ab183797)Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized frozen Mouse hippocampus tissue labeling Glutamate Receptor 1 (AMPA subtype) with ab183797 at 1/100 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Cytoplasm staining on mouse hippocampus was observed. The nuclear counterstain is DAPI (blue).
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab150077 at 1/1000 dilution.
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![Immunoprecipitation - Anti-Glutamate Receptor 1 (AMPA subtype) antibody [EPR19522] (ab183797)](https://www.abcam.com/ps/products/183/ab183797/Images/ab183797-263063-anti-glutamate-receptor-1-ampa-subtype-antibody-epr19522-immunoprecipitation.jpg)
Immunoprecipitation - Anti-Glutamate Receptor 1 (AMPA subtype) antibody [EPR19522] (ab183797)Glutamate Receptor 1 (AMPA subtype) was immunoprecipitated from 0.35 mg of Mouse brain whole cell lysate with ab183797 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab183797 at 1/1000 dilution. VeriBlot for IP Detection Reaction (HRP) (ab131366), was used for detection at 1/10000 dilution.
Lane 1: Mouse brain whole cell lysate, 10ug (Input).
Lane 2: ab183797 IP in Mouse brain whole cell lysate.
Lane 3: Rabbit IgG,monoclonal [EPR25A]-Isotype Control (ab172730) instead of ab183797 in Mouse brain whole cell lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 3 minutes.
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![Anti-Glutamate Receptor 1 (AMPA subtype) antibody [EPR19522] (ab183797)](https://www.abcam.com/ps/products/183/ab183797/Images/ab183797-11-benefits-of-recombinant-antibodies.png)
Anti-Glutamate Receptor 1 (AMPA subtype) antibody [EPR19522] (ab183797)
Protocols
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (11)
ab183797 has been referenced in 11 publications.
- Zhang D et al. Photobiomodulation Therapy Ameliorates Glutamatergic Dysfunction in Mice with Chronic Unpredictable Mild Stress-Induced Depression. Oxid Med Cell Longev 2021:6678276 (2021). PubMed: 33859781
- Fu R et al. P2X4 receptor in the dorsal horn contributes to BDNF/TrkB and AMPA receptor activation in the pathogenesis of remifentanil-induced postoperative hyperalgesia in rats. Neurosci Lett 750:135773 (2021). PubMed: 33639220
- Li Y et al. A Role for Transmembrane Protein 16C/Slack Impairment in Excitatory Nociceptive Synaptic Plasticity in the Pathogenesis of Remifentanil-induced Hyperalgesia in Rats. Neurosci Bull 37:669-683 (2021). PubMed: 33779892
- Song X et al. ?-opioid receptor agonist, U50488H, inhibits pyroptosis through NLRP3 via the Ca2+/CaMKII/CREB signaling pathway and improves synaptic plasticity in APP/PS1 mice. Mol Med Rep 24:N/A (2021). PubMed: 34036389
- Batool S et al. Spatiotemporal Patterns of Menin Localization in Developing Murine Brain: Co-Expression with the Elements of Cholinergic Synaptic Machinery. Cells 10:N/A (2021). PubMed: 34065662