Recombinant Anti-Glutamate Receptor 1 (AMPA subtype) antibody [EPR19522] - BSA and Azide free (ab250709)
![Western blot - Anti-Glutamate Receptor 1 (AMPA subtype) antibody [EPR19522] - BSA and Azide free (ab250709)](https://www.abcam.com/ps/products/250/ab250709/Images/ab250709-447174-anti-glutamate-receptor-1-ampa-subtype-antibody-epr19522-bsa-and-azide-free-western-blot.jpg "Western blot - Anti-Glutamate Receptor 1 (AMPA subtype) antibody [EPR19522] - BSA and Azide free (ab250709)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR19522] to Glutamate Receptor 1 (AMPA subtype) - BSA and Azide free
- Suitable for: IP, IHC-Fr, IHC-P, WB
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-Glutamate Receptor 1 (AMPA subtype) antibody [EPR19522] - BSA and Azide free
See all Glutamate Receptor 1 (AMPA subtype) primary antibodies -
Description
Rabbit monoclonal [EPR19522] to Glutamate Receptor 1 (AMPA subtype) - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: IP, IHC-Fr, IHC-P, WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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General notes
ab250709 is the carrier-free version of ab183797.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR19522 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab250709 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IP |
Use at an assay dependent concentration.
|
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| IHC-Fr |
Use at an assay dependent concentration.
Antigen retrieval: Heated citrate solution (10mM citrate pH 6.0 + 0.05% Tween-20). IHC is recommended for mouse and rat only. |
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| IHC-P |
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
IHC is recommended for mouse and rat only.
|
|
| WB |
Use at an assay dependent concentration. Detects a band of approximately 102 kDa (predicted molecular weight: 102 kDa).
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| Notes |
|---|
|
IP
Use at an assay dependent concentration. |
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IHC-Fr
Use at an assay dependent concentration. Antigen retrieval: Heated citrate solution (10mM citrate pH 6.0 + 0.05% Tween-20). IHC is recommended for mouse and rat only. |
|
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. IHC is recommended for mouse and rat only.
|
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WB
Use at an assay dependent concentration. Detects a band of approximately 102 kDa (predicted molecular weight: 102 kDa). |
Target
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Function
Ionotropic glutamate receptor. L-glutamate acts as an excitatory neurotransmitter at many synapses in the central nervous system. Binding of the excitatory neurotransmitter L-glutamate induces a conformation change, leading to the opening of the cation channel, and thereby converts the chemical signal to an electrical impulse. The receptor then desensitizes rapidly and enters a transient inactive state, characterized by the presence of bound agonist. -
Tissue specificity
Widely expressed in brain. -
Sequence similarities
Belongs to the glutamate-gated ion channel (TC 1.A.10.1) family. GRIA1 subfamily. -
Post-translational
modificationsPalmitoylated. Depalmitoylated upon glutamate stimulation. Cys-603 palmitoylation leads to Golgi retention and decreased cell surface expression. In contrast, Cys-829 palmitoylation does not affect cell surface expression but regulates stimulation-dependent endocytosis. -
Cellular localization
Cell membrane. Endoplasmic reticulum membrane. Cell junction > synapse > postsynaptic cell membrane. Interaction with CACNG2 promotes cell surface expression. - Information by UniProt
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Database links
- Entrez Gene: 2890 Human
- Entrez Gene: 14799 Mouse
- Entrez Gene: 50592 Rat
- Omim: 138248 Human
- SwissProt: P42261 Human
- SwissProt: P23818 Mouse
- SwissProt: P19490 Rat
- Unigene: 519693 Human
see all -
Alternative names
- GLUR 1 antibody
- GLUR A antibody
- AMPA 1 antibody
see all
Images
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Western blot - Anti-Glutamate Receptor 1 (AMPA subtype) antibody [EPR19522] - BSA and Azide free (ab250709)All lanes : Anti-Glutamate Receptor 1 (AMPA subtype) antibody [EPR19522] (ab183797) at 1/5000 dilution
Lane 1 : Mouse Glutamate Receptor 1 fragment recombinant protein
Lane 2 : Mouse Glutamate Receptor 2 fragment recombinant protein
Lysates/proteins at 0.01 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 102 kDaThis data was developed using ab183797, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
Exposure times: Lane 1: 2 seconds; Lane 2: 3 minutes.
Mouse Glutamate Receptor 1 fragment recombinant protein contains aa19-184 with a GST/His-Tag®. Mouse Glutamate Receptor 2 fragment recombinant protein contains aa25-288 with a His-Tag®.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glutamate Receptor 1 (AMPA subtype) antibody [EPR19522] - BSA and Azide free (ab250709)](https://www.abcam.com/ps/products/250/ab250709/Images/ab250709-8-anti-glutamate-receptor-1-ampa-subtype-antibody-epr19522-bsa-and-azide-free-immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glutamate Receptor 1 (AMPA subtype) antibody [EPR19522] - BSA and Azide free (ab250709)This data was developed using ab183797, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Mouse hippocampus tissue labeling Glutamate Receptor 1 (AMPA subtype) with ab183797 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasm staining on mouse hippocampus was observed [PMID: 15723058]. Counter stained with Hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab97051 at 1/500 dilution. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. -
![Immunohistochemistry (Frozen sections) - Anti-Glutamate Receptor 1 (AMPA subtype) antibody [EPR19522] - BSA and Azide free (ab250709)](https://www.abcam.com/ps/products/250/ab250709/Images/ab250709-3-anti-glutamate-receptor-1-ampa-subtype-antibody-epr19522-bsa-and-azide-free-immunohistochemistry-frozen-sections.jpg)
Immunohistochemistry (Frozen sections) - Anti-Glutamate Receptor 1 (AMPA subtype) antibody [EPR19522] - BSA and Azide free (ab250709)This data was developed using ab183797, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized frozen Mouse hippocampus tissue labeling Glutamate Receptor 1 (AMPA subtype) with ab183797 at 1/100 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Cytoplasm staining on mouse hippocampus was observed. The nuclear counterstain is DAPI (blue). Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab150077 secondary antibody at 1/1000 dilution. -
![Western blot - Anti-Glutamate Receptor 1 (AMPA subtype) antibody [EPR19522] - BSA and Azide free (ab250709)](https://www.abcam.com/ps/products/250/ab250709/Images/ab250709-447040-anti-glutamate-receptor-1-ampa-subtype-antibody-epr19522-bsa-and-azide-free-western-blot.jpg)
Western blot - Anti-Glutamate Receptor 1 (AMPA subtype) antibody [EPR19522] - BSA and Azide free (ab250709)All lanes : Anti-Glutamate Receptor 1 (AMPA subtype) antibody [EPR19522] (ab183797) at 1/1000 dilution
Lane 1 : Mouse brain lysate
Lane 2 : Rat liver lysate
Lane 3 : Mouse hippocampus lysate
Lane 4 : Mouse cerebellum lysate
Lane 5 : Rat brain lysate
Lane 6 : Rat cerebellum lysate
Lane 7 : Rat hippocampus lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 102 kDa
Observed band size: 102 kDaThis data was developed using ab183797, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
Exposure times: Lanes 1-2: 30 seconds; Lanes 3-7: 15 seconds.
Rodent Glutamate Receptor 1 is widely expressed in brain and represents the predominant excitatory neurotransmitter system but not in liver.
Negative control: Rat liver (PMID: 2480522).
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![Western blot - Anti-Glutamate Receptor 1 (AMPA subtype) antibody [EPR19522] - BSA and Azide free (ab250709)](https://www.abcam.com/ps/products/250/ab250709/Images/ab250709-446677-anti-glutamate-receptor-1-ampa-subtype-antibody-epr19522-bsa-and-azide-free-western-blot.jpg)
Western blot - Anti-Glutamate Receptor 1 (AMPA subtype) antibody [EPR19522] - BSA and Azide free (ab250709)All lanes : Anti-Glutamate Receptor 1 (AMPA subtype) antibody [EPR19522] (ab183797) at 1/1000 dilution
Lane 1 : Human cerebellum lysate
Lane 2 : Human muscle lysate
Lane 3 : Human fetal brain lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution
Predicted band size: 102 kDa
Observed band size: 102 kDa
Exposure time: 3 minutesThis data was developed using ab183797, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
Human Glutamate Receptor 1 is widely expressed in brain and represents the predominant excitatory neurotransmitter system but not in muscle.
Negative control: human muscle (PMID: 1652753).
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glutamate Receptor 1 (AMPA subtype) antibody [EPR19522] - BSA and Azide free (ab250709)](https://www.abcam.com/ps/products/250/ab250709/Images/ab250709-7-anti-glutamate-receptor-1-ampa-subtype-antibody-epr19522-bsa-and-azide-free-immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glutamate Receptor 1 (AMPA subtype) antibody [EPR19522] - BSA and Azide free (ab250709)This data was developed using ab183797, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Mouse liver tissue labeling Glutamate Receptor 1 (AMPA subtype) with ab183797 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. No staining on mouse liver is observed. Counter stained with Hematoxylin. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glutamate Receptor 1 (AMPA subtype) antibody [EPR19522] - BSA and Azide free (ab250709)](https://www.abcam.com/ps/products/250/ab250709/Images/ab250709-5-anti-glutamate-receptor-1-ampa-subtype-antibody-epr19522-bsa-and-azide-free-immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glutamate Receptor 1 (AMPA subtype) antibody [EPR19522] - BSA and Azide free (ab250709)This data was developed using ab183797, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Rat hippocampus tissue labeling Glutamate Receptor 1 (AMPA subtype) with ab183797 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasm staining on rat hippocampus is observed [PMID: 15723058]. Counter stained with Hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab97051 at 1/500 dilution. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glutamate Receptor 1 (AMPA subtype) antibody [EPR19522] - BSA and Azide free (ab250709)](https://www.abcam.com/ps/products/250/ab250709/Images/ab250709-4-anti-glutamate-receptor-1-ampa-subtype-antibody-epr19522-bsa-and-azide-free-immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glutamate Receptor 1 (AMPA subtype) antibody [EPR19522] - BSA and Azide free (ab250709)This data was developed using ab183797, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Rat stomach tissue labeling Glutamate Receptor 1 (AMPA subtype) with ab183797 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. No staining on rat stomach is observed. Counter stained with Hematoxylin. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. -
![Immunohistochemistry (Frozen sections) - Anti-Glutamate Receptor 1 (AMPA subtype) antibody [EPR19522] - BSA and Azide free (ab250709)](https://www.abcam.com/ps/products/250/ab250709/Images/ab250709-2-anti-glutamate-receptor-1-ampa-subtype-antibody-epr19522-bsa-and-azide-free-immunohistochemistry-frozen-sections.jpg)
Immunohistochemistry (Frozen sections) - Anti-Glutamate Receptor 1 (AMPA subtype) antibody [EPR19522] - BSA and Azide free (ab250709)This data was developed using ab183797, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized frozen Mouse hippocampus tissue labeling Glutamate Receptor 1 (AMPA subtype) with ab183797 at 1/100 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Cytoplasm staining on mouse hippocampus was observed. The nuclear counterstain is DAPI (blue). Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab150077 at 1/1000 dilution. -
![Immunoprecipitation - Anti-Glutamate Receptor 1 (AMPA subtype) antibody [EPR19522] - BSA and Azide free (ab250709)](https://www.abcam.com/ps/products/250/ab250709/Images/ab250709-1-anti-glutamate-receptor-1-ampa-subtype-antibody-epr19522-bsa-and-azide-free-immunoprecipitation.jpg)
Immunoprecipitation - Anti-Glutamate Receptor 1 (AMPA subtype) antibody [EPR19522] - BSA and Azide free (ab250709)This data was developed using ab183797, the same antibody clone in a different buffer formulation.Glutamate Receptor 1 (AMPA subtype) was immunoprecipitated from 0.35 mg of Mouse brain whole cell lysate with ab183797 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab183797 at 1/1000 dilution. VeriBlot for IP Detection Reaction (HRP) (ab131366), was used for detection at 1/10000 dilution. Lane 1: Mouse brain whole cell lysate, 10ug (Input). Lane 2: ab183797 IP in Mouse brain whole cell lysate. Lane 3: Rabbit IgG,monoclonal [EPR25A]-Isotype Control (ab172730) instead of ab183797 in Mouse brain whole cell lysate. Blocking and dilution buffer and concentration: 5% NFDM/TBST. Exposure time: 3 minutes. -
![Anti-Glutamate Receptor 1 (AMPA subtype) antibody [EPR19522] - BSA and Azide free (ab250709)](https://www.abcam.com/ps/products/250/ab250709/Images/ab250709-6-benefits-of-recombinant-antibodies.png)
Anti-Glutamate Receptor 1 (AMPA subtype) antibody [EPR19522] - BSA and Azide free (ab250709)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
Certificate of Compliance
References (0)
ab250709 has not yet been referenced specifically in any publications.
