Recombinant Anti-Glutamate receptor 4 antibody [EPR24283-69] (ab280199)
![Western blot - Anti-Metabotropic Glutamate Receptor 4/MGLUR4 antibody [EPR24283-69] (ab280199)](https://www.abcam.com/ps/products/280/ab280199/Images/ab280199-454311-anti-glutamate-receptor-4antibody-epr2428369-western-blot-lysate1.jpg "Western blot - Anti-Metabotropic Glutamate Receptor 4/MGLUR4 antibody [EPR24283-69] (ab280199)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR24283-69] to Glutamate receptor 4
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-Glutamate receptor 4 antibody [EPR24283-69]
See all Glutamate receptor 4 primary antibodies -
Description
Rabbit monoclonal [EPR24283-69] to Glutamate receptor 4 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details
Unsuitable for: Flow Cyt,ICC/IF,IHC-Fr or IP -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Rat cerebellum and brain; Mouse cerebellum and brain; Human cerebellum tissue lysates. IHC-P: Mouse cerebrum; Rat cerebrum; Human cerebrum.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 59.94% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR24283-69 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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HRP detection kit
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab280199 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/1000. Predicted molecular weight: 102 kDa.
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| IHC-P |
1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
|
| Notes |
|---|
|
WB
1/1000. Predicted molecular weight: 102 kDa. |
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IHC-P
1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Target
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Function
Receptor for glutamate that functions as ligand-gated ion channel in the central nervous system and plays an important role in excitatory synaptic transmission. L-glutamate acts as an excitatory neurotransmitter at many synapses in the central nervous system. Binding of the excitatory neurotransmitter L-glutamate induces a conformation change, leading to the opening of the cation channel, and thereby converts the chemical signal to an electrical impulse. The receptor then desensitizes rapidly and enters a transient inactive state, characterized by the presence of bound agonist. In the presence of CACNG4 or CACNG7 or CACNG8, shows resensitization which is characterized by a delayed accumulation of current flux upon continued application of glutamate. -
Sequence similarities
Belongs to the glutamate-gated ion channel (TC 1.A.10.1) family. GRIA4 subfamily. -
Domain
The M4 transmembrane segment mediates tetramerization and is required for cell surface expression. -
Post-translational
modificationsPalmitoylated. Depalmitoylated upon glutamate stimulation. Cys-611 palmitoylation leads to Golgi retention and decreased cell surface expression. In contrast, Cys-837 palmitoylation does not affect cell surface expression but regulates stimulation-dependent endocytosis.
Phosphorylated at Ser-862 by PRKCG; phosphorylation increases plasma membrane-associated GRI4 expression. -
Cellular localization
Cell membrane. Cell junction, synapse, postsynaptic cell membrane. Cell projection, dendrite. Interaction with CNIH2, CNIH3 and PRKCG promotes cell surface expression. - Information by UniProt
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Database links
- Entrez Gene: 2893 Human
- SwissProt: P48058 Human
- Unigene: 503743 Human
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Alternative names
- AMPA 4 antibody
- AMPA-selective glutamate receptor 4 antibody
- GluA4 antibody
see all
Images
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Western blot - Anti-Metabotropic Glutamate Receptor 4/MGLUR4 antibody [EPR24283-69] (ab280199)All lanes : Anti-Glutamate receptor 4 antibody [EPR24283-69] (ab280199) at 1/1000 dilution
Lane 1 : Human cerebellum tissue lysate at 20 µg
Lane 2 : Human liver tissue lysate at 40 µg
Secondary
All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Predicted band size: 102 kDa
Observed band size: 100 kDa why is the actual band size different from the predicted?Blocking and diluting buffer and concentration: 5% NFDM/TBST
Samples are non-boiled as boiling may cause protein aggregates.
The expression pattern is consistent to previous reports (PMID:12657691, PMID:26985043, PMID:20549383)
Negative control: human liver (PMID:1372042)
Exposure time: 3.25 seconds
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Metabotropic Glutamate Receptor 4/MGLUR4 antibody [EPR24283-69] (ab280199)](https://www.abcam.com/ps/products/280/ab280199/Images/ab280199-3-anti-glutamate-receptor-4antibody-epr2428369-immunohistochemistry-human-cerebrum.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Metabotropic Glutamate Receptor 4/MGLUR4 antibody [EPR24283-69] (ab280199)Immunohistochemical analysis of paraffin-embedded Human cerebrum tissue labeling Glutamate receptor 4 with ab280199 at 1/500 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on human cerebrum. The section was incubated with ab280199 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins
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![Western blot - Anti-Metabotropic Glutamate Receptor 4/MGLUR4 antibody [EPR24283-69] (ab280199)](https://www.abcam.com/ps/products/280/ab280199/Images/ab280199-454310-anti-glutamate-receptor-4antibody-epr2428369-western-blot-lysate.jpg)
Western blot - Anti-Metabotropic Glutamate Receptor 4/MGLUR4 antibody [EPR24283-69] (ab280199)All lanes : Anti-Glutamate receptor 4 antibody [EPR24283-69] (ab280199) at 1/1000 dilution
Lane 1 : Rat cerebellum tissue lysate at 20 µg
Lane 2 : Rat brain tissue lysate at 20 µg
Lane 3 : Rat liver tissue lysate at 40 µg
Lane 4 : Mouse cerebellum tissue lysate at 20 µg
Lane 5 : Mouse brain tissue lysate at 20 µg
Lane 6 : Mouse liver tissue lysate at 40 µg
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution
Predicted band size: 102 kDa
Observed band size: 100 kDa why is the actual band size different from the predicted?Blocking and diluting buffer and concentration: 5% NFDM/TBST
Samples are non-boiled as boiling may cause protein aggregates.
The expression pattern is consistent to previous reports (PMID:12657691, PMID:26985043, PMID:20549383)
Negative control: liver (PMID:1372042)
Exposure time: 7.75 seconds
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Metabotropic Glutamate Receptor 4/MGLUR4 antibody [EPR24283-69] (ab280199)](https://www.abcam.com/ps/products/280/ab280199/Images/ab280199-1-anti-glutamate-receptor-4antibody-epr2428369-immunohistochemistry-mouse-cerebrum.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Metabotropic Glutamate Receptor 4/MGLUR4 antibody [EPR24283-69] (ab280199)Immunohistochemical analysis of paraffin-embedded Mouse cerebrum tissue labeling Glutamate receptor 4 with ab280199 at 1/2000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on mouse cerebrum. (PMID: 20662939) The section was incubated with ab280199 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Metabotropic Glutamate Receptor 4/MGLUR4 antibody [EPR24283-69] (ab280199)](https://www.abcam.com/ps/products/280/ab280199/Images/ab280199-2-anti-glutamate-receptor-4antibody-epr2428369-immunohistochemistry-rat-cerebrum.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Metabotropic Glutamate Receptor 4/MGLUR4 antibody [EPR24283-69] (ab280199)Immunohistochemical analysis of paraffin-embedded Rat cerebrum tissue labeling Glutamate receptor 4 with ab280199 at 1/2000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on rat cerebrum. The section was incubated with ab280199 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Metabotropic Glutamate Receptor 4/MGLUR4 antibody [EPR24283-69] (ab280199)](https://www.abcam.com/ps/products/280/ab280199/Images/ab280199-4-anti-glutamate-receptor-4antibody-epr2428369-immunohistochemistry-mouse-liver.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Metabotropic Glutamate Receptor 4/MGLUR4 antibody [EPR24283-69] (ab280199)Immunohistochemical analysis of paraffin-embedded Mouse liver tissue labeling Glutamate receptor 4 with ab280199 at 1/2000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Negative control: No staining on mouse liver. (PMID: 1372042) The section was incubated with ab280199 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (0)
ab280199 has not yet been referenced specifically in any publications.