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RecombinantRabMAb

Recombinant Anti-Glutamate receptor 4 antibody [EPR24283-69] - BSA and Azide free (ab280206)

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Western blot - Anti-Metabotropic Glutamate Receptor 4/MGLUR4 antibody [EPR24283-69] - BSA and Azide free (ab280206)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Metabotropic Glutamate Receptor 4/MGLUR4 antibody [EPR24283-69] - BSA and Azide free (ab280206)
  • Western blot - Anti-Metabotropic Glutamate Receptor 4/MGLUR4 antibody [EPR24283-69] - BSA and Azide free (ab280206)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Metabotropic Glutamate Receptor 4/MGLUR4 antibody [EPR24283-69] - BSA and Azide free (ab280206)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Metabotropic Glutamate Receptor 4/MGLUR4 antibody [EPR24283-69] - BSA and Azide free (ab280206)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Metabotropic Glutamate Receptor 4/MGLUR4 antibody [EPR24283-69] - BSA and Azide free (ab280206)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR24283-69] to Glutamate receptor 4 - BSA and Azide free
  • Suitable for: WB, IHC-P
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Unconjugated

Overview

  • Product name

    Anti-Glutamate receptor 4 antibody [EPR24283-69] - BSA and Azide free
    See all Glutamate receptor 4 primary antibodies

  • Description

    Rabbit monoclonal [EPR24283-69] to Glutamate receptor 4 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-Pmore details
    Unsuitable for: Flow Cyt,ICC/IF,IHC-Fr or IP

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Rat cerebellum and brain; Mouse cerebellum and brain; Human cerebellum tissue lysates. IHC-P: Mouse cerebrum; Rat cerebrum; Human cerebrum.

  • General notes

    ab280206 is the carrier-free version of ab280199.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab280206 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
Use at an assay dependent concentration. Predicted molecular weight: 102 kDa.
IHC-P
Use at an assay dependent concentration.
Notes
WB
Use at an assay dependent concentration. Predicted molecular weight: 102 kDa.
IHC-P
Use at an assay dependent concentration.
Application notes
Is unsuitable for Flow Cyt,ICC/IF,IHC-Fr or IP.

Target

  • Function

    Receptor for glutamate that functions as ligand-gated ion channel in the central nervous system and plays an important role in excitatory synaptic transmission. L-glutamate acts as an excitatory neurotransmitter at many synapses in the central nervous system. Binding of the excitatory neurotransmitter L-glutamate induces a conformation change, leading to the opening of the cation channel, and thereby converts the chemical signal to an electrical impulse. The receptor then desensitizes rapidly and enters a transient inactive state, characterized by the presence of bound agonist. In the presence of CACNG4 or CACNG7 or CACNG8, shows resensitization which is characterized by a delayed accumulation of current flux upon continued application of glutamate.

  • Sequence similarities

    Belongs to the glutamate-gated ion channel (TC 1.A.10.1) family. GRIA4 subfamily.

  • Domain

    The M4 transmembrane segment mediates tetramerization and is required for cell surface expression.

  • Post-translational
    modifications

    Palmitoylated. Depalmitoylated upon glutamate stimulation. Cys-611 palmitoylation leads to Golgi retention and decreased cell surface expression. In contrast, Cys-837 palmitoylation does not affect cell surface expression but regulates stimulation-dependent endocytosis.
    Phosphorylated at Ser-862 by PRKCG; phosphorylation increases plasma membrane-associated GRI4 expression.

  • Cellular localization

    Cell membrane. Cell junction, synapse, postsynaptic cell membrane. Cell projection, dendrite. Interaction with CNIH2, CNIH3 and PRKCG promotes cell surface expression.
  • Information by UniProt

  • Database links

    • Alternative names

      • AMPA 4 antibody
      • AMPA-selective glutamate receptor 4 antibody
      • GluA4 antibody
      • GluR-4 antibody
      • GluR-D antibody
      • GluR4 antibody
      • Glutamate receptor 4 antibody
      • Glutamate receptor ionotropic antibody
      • Gria4 antibody
      • GRIA4_HUMAN antibody
      see all

    Images

    • Western blot - Anti-Metabotropic Glutamate Receptor 4/MGLUR4 antibody [EPR24283-69] - BSA and Azide free (ab280206)
      Western blot - Anti-Metabotropic Glutamate Receptor 4/MGLUR4 antibody [EPR24283-69] - BSA and Azide free (ab280206)
      All lanes : Anti-Glutamate receptor 4 antibody [EPR24283-69] (ab280199) at 1/1000 dilution

      Lane 1 : Human cerebellum tissue lysate at 20 µg
      Lane 2 : Human liver tissue lysate at 40 µg

      Secondary
      All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

      Predicted band size: 102 kDa
      Observed band size: 100 kDa why is the actual band size different from the predicted?



      This data was developed using ab280199, the same antibody clone in a different buffer formulation. 

      Blocking and diluting buffer and concentration: 5% NFDM/TBST

      Samples are non-boiled as boiling may cause protein aggregates.

      The expression pattern is consistent to previous reports (PMID:12657691, PMID:26985043, PMID:20549383)

      Negative control: human liver (PMID:1372042)

      Exposure time: 3.25 seconds

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Metabotropic Glutamate Receptor 4/MGLUR4 antibody [EPR24283-69] - BSA and Azide free (ab280206)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Metabotropic Glutamate Receptor 4/MGLUR4 antibody [EPR24283-69] - BSA and Azide free (ab280206)

      This data was developed using ab280199, the same antibody clone in a different buffer formulation.

      Immunohistochemical analysis of paraffin-embedded Human cerebrum tissue labeling Glutamate receptor 4 with ab280199 at 1/500 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on human cerebrum. The section was incubated with ab280199 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

      Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

      Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

    • Western blot - Anti-Metabotropic Glutamate Receptor 4/MGLUR4 antibody [EPR24283-69] - BSA and Azide free (ab280206)
      Western blot - Anti-Metabotropic Glutamate Receptor 4/MGLUR4 antibody [EPR24283-69] - BSA and Azide free (ab280206)
      All lanes : Anti-Glutamate receptor 4 antibody [EPR24283-69] (ab280199) at 1/1000 dilution

      Lane 1 : Rat cerebellum tissue lysate at 20 µg
      Lane 2 : Rat brain tissue lysate at 20 µg
      Lane 3 : Rat liver tissue lysate at 40 µg
      Lane 4 : Mouse cerebellum tissue lysate at 20 µg
      Lane 5 : Mouse brain tissue lysate at 20 µg
      Lane 6 : Mouse liver tissue lysate at 40 µg

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

      Predicted band size: 102 kDa
      Observed band size: 100 kDa why is the actual band size different from the predicted?



      This data was developed using ab280199, the same antibody clone in a different buffer formulation. 

      Blocking and diluting buffer and concentration: 5% NFDM/TBST

      Samples are non-boiled as boiling may cause protein aggregates.

      The expression pattern is consistent to previous reports (PMID:12657691, PMID:26985043, PMID:20549383)

      Negative control:  liver (PMID:1372042)

      Exposure time: 7.75 seconds

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Metabotropic Glutamate Receptor 4/MGLUR4 antibody [EPR24283-69] - BSA and Azide free (ab280206)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Metabotropic Glutamate Receptor 4/MGLUR4 antibody [EPR24283-69] - BSA and Azide free (ab280206)

      This data was developed using ab280199, the same antibody clone in a different buffer formulation.

      Immunohistochemical analysis of paraffin-embedded Mouse cerebrum tissue labeling Glutamate receptor 4 with ab280199 at 1/2000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on mouse cerebrum. (PMID: 20662939) The section was incubated with ab280199 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

      Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

      Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Metabotropic Glutamate Receptor 4/MGLUR4 antibody [EPR24283-69] - BSA and Azide free (ab280206)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Metabotropic Glutamate Receptor 4/MGLUR4 antibody [EPR24283-69] - BSA and Azide free (ab280206)

      This data was developed using ab280199, the same antibody clone in a different buffer formulation.

      Immunohistochemical analysis of paraffin-embedded Rat cerebrum tissue labeling Glutamate receptor 4 with ab280199 at 1/2000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on rat cerebrum. The section was incubated with ab280199 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

      Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

      Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Metabotropic Glutamate Receptor 4/MGLUR4 antibody [EPR24283-69] - BSA and Azide free (ab280206)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Metabotropic Glutamate Receptor 4/MGLUR4 antibody [EPR24283-69] - BSA and Azide free (ab280206)

      This data was developed using ab280199, the same antibody clone in a different buffer formulation.

      Immunohistochemical analysis of paraffin-embedded Mouse liver tissue labeling Glutamate receptor 4 with ab280199 at 1/2000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Negative control: No staining on mouse liver. (PMID: 1372042) The section was incubated with ab280199 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

      Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

      Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (0)

    Publishing research using ab280206? Please let us know so that we can cite the reference in this datasheet.

    ab280206 has not yet been referenced specifically in any publications.

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