Recombinant Anti-glutathione S transferase Omega 1/p28 antibody [EPR8370(B)] - BSA and Azide free (ab248304)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR8370(B)] to glutathione S transferase Omega 1/p28 - BSA and Azide free
- Suitable for: IHC-P, WB
- Knockout validated
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-glutathione S transferase Omega 1/p28 antibody [EPR8370(B)] - BSA and Azide free
See all glutathione S transferase Omega 1/p28 primary antibodies -
Description
Rabbit monoclonal [EPR8370(B)] to glutathione S transferase Omega 1/p28 - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details
Unsuitable for: Flow Cyt or IP -
Species reactivity
Reacts with: Human
Predicted to work with: Rat -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: HEK293T, Jurkat, HepG2 and BxPC-3 cell lysates; Human fetal liver lysate. IHC-P: Human colon tissue.
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General notes
ab248304 is the carrier-free version of ab129106.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR8370(B) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Conjugation kits
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Isotype control
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KO cell lines
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KO cell lysates
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab248304 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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WB |
Use at an assay dependent concentration. Detects a band of approximately 31 kDa (predicted molecular weight: 28 kDa).
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Notes |
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IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
WB
Use at an assay dependent concentration. Detects a band of approximately 31 kDa (predicted molecular weight: 28 kDa). |
Target
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Function
Exhibits glutathione-dependent thiol transferase and dehydroascorbate reductase activities. Has S-(phenacyl)glutathione reductase activity. Has also glutathione S-transferase activity. Participates in the biotransformation of inorganic arsenic and reduces monomethylarsonic acid (MMA) and dimethylarsonic acid. -
Tissue specificity
Ubiquitous. Highest expression in liver, pancreas, skeletal muscle, spleen, thymus, colon, blood leukocyte and heart. Lowest expression in brain, placenta and lung. -
Sequence similarities
Belongs to the GST superfamily. Omega family.
Contains 1 GST C-terminal domain.
Contains 1 GST N-terminal domain. -
Cellular localization
Cytoplasm > cytosol. - Information by UniProt
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Database links
- Entrez Gene: 9446 Human
- Entrez Gene: 114846 Rat
- Omim: 605482 Human
- SwissProt: P78417 Human
- SwissProt: Q9Z339 Rat
- Unigene: 190028 Human
- Unigene: 25166 Rat
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Alternative names
- AA407097 antibody
- AI194287 antibody
- AU018802 antibody
see all
Images
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All lanes : Anti-glutathione S transferase Omega 1/p28 antibody [EPR8370(B)] (ab129106) at 1/1000 dilution
Lane 1 : Wild-type HEK293T cell lysate
Lane 2 : GSTO1 knockout HEK293T cell lysate
Lane 3 : Jurkat cell lysate
Lane 4 : BxPC-3 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution
Predicted band size: 28 kDa
Observed band size: 28 kDaThis data was developed using ab129106, the same antibody clone in a different buffer formulation.
Lanes 1-4: Merged signal (red and green). Green - ab129106 observed at 28 kDa. Red - loading control ab8245 observed at 36 kDa.
ab129106 Anti-glutathione S transferase Omega 1/p28 antibody [EPR8370(B)] was shown to specifically react with glutathione S transferase Omega 1/p28 in wild-type HEK293T cells. Loss of signal was observed when knockout cell line ab266277 (knockout cell lysate ab257982) was used. Wild-type and glutathione S transferase Omega 1/p28 knockout samples were subjected to SDS-PAGE. ab129106 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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All lanes : Anti-glutathione S transferase Omega 1/p28 antibody [EPR8370(B)] (ab129106) at 1/1000 dilution
Lane 1 : Human fetal liver lysate
Lane 2 : BxPC3 lysate
Lane 3 : HepG2 lysate
Lane 4 : HeLa lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-Rabbit HRP at 1/2000 dilution
Predicted band size: 28 kDaThis data was developed using ab129106, the same antibody clone in a different buffer formulation.
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This data was developed using ab129106, the same antibody clone in a different buffer formulation.ab129106, at 1/100 dilution, staining glutathione S transferase Omega 1/p28 in Paraffin-embedded Human colon tissue by Immunohistochemistry. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
Certificate of Compliance
References (0)
ab248304 has not yet been referenced specifically in any publications.