Anti-Glycophorin A + B antibody [HIR2] (ab15009)

We do not have a CD71 antibody that has been tested in ELISA or any other kind of capture assay, but the monoclonal antibody ab116015 is specific for an extracellular epitope and can at least identify CD71 on cells. Capturing reticulocytes with the magnetic beads that you plan on conjugating to the antibody has not been attempted, as far as we know. Please note that the formulation of the antibody contains 1% BSA, which may interfere with the conjugation.

Click here (or use the following: https://www.abcam.com/Transferrin-Receptor-antibody-DF-1513-Workshop-IV-ab116015.html).

We have a "clean-up" kit that will remove the BSA by purifying the antibody over a Protein A column, if you are interested.

Click here (or use the following: https://www.abcam.com/Antibody-Purification-Kit-Protein-A-ab102784.html).

Please let me know if you have any questions.

Thank you for your patience. We have not determined the light chain type of ab15009 in house, but I have found this clone in the literature listed as being IgG2b kappa. You could therefore use ab18421 or ab18469 as an isotype control. Please let me know if you need any additional information!

Thank you for contacting us.

We have 4, FITC conjugated anti Glycophorin A antibodies.

https://www.abcam.com/Glycophorin-A-antibody-YTH89-1-FITC-ab28082.html
https://www.abcam.com/Glycophorin-A-antibody-FITC-ab112201.html
https://www.abcam.com/Glycophorin-A-antibody-HIR2-FITC-Aminoterminal-end-ab52452.html
https://www.abcam.com/Glycophorin-A-antibody-CLB-AME-1-FITC-ab51531.html

3 Phycoerythrin conjugated abs

https://www.abcam.com/Glycophorin-A-antibody-CLB-AME-1-Phycoerythrin-ab51632.html
https://www.abcam.com/Glycophorin-A-antibody-YTH89-1-Phycoerythrin-ab33387.html
https://www.abcam.com/Glycophorin-A-B-antibody-HIR2-Phycoerythrin-ab26016.html

The price for these antibodies for 1 vial would be

ab28082; £283 per 100 microgram
ab51632: £227 per 500 micro litre
ab112201; £227 per 100 microgram
ab33387: £227 50 tests
ab52452: £227 per 100 tests
ab51531; £227 per 100 tests
ab26016: £227 per 100 tests

ab26016 the concentration is 0.01mg/ml

All other information is present on the datasheets. The product will be dispatched same day for next day delivery.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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https://www.abcam.com/abreviews

Thank you for contacting us.

Here is the paper I mentioned in our conversation. It discusses the technique you plan on using, magnetic bead isolation.

The isolation of reticulocyte-free human red blood cells. Exp Biol Med (Maywood). 2007 Dec;232(11):1470-6. PMID: 18040072

http://ebm.rsmjournals.com/content/232/11/1470.full

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Thank you for your reply.

Unfortunately we only have one anti-Glycophorin A antibody where we also have the blocking peptide available. This is goat polyclonalab40844 (blocking peptide is catalogue number ab45821) which is raised against a peptide taken from the C-terminal end of human Glycophorin A. This is not in the extracellular domain of Glycophorin A.

We have not tested this antibody in ICC yet and to our knowledge, neither has any of our customers. This does not mean that it will not work, simply that we have not tested it as yet. As the epitope recognised by theantibody is cytoplasmic, some permiabilisationwill be required.

If you would be interested intryingab40844for yourICC experiment, you would be eligible forthe testing discount scheme that we run. This would involve you purchasingab40844, testingit in ICC, then letting us knowof the results (regardless of if they are positive or negative) through an Abreview. You would then be entitledto a free primary antibody of your choicefrom our catalogue.More information on this scheme can be found from the following link:

https://www.abcam.com/collaboratordiscount

If you would like to participatein this scheme please do let me know as a discount code needs to be issued prior to purchase of ab40844.

Alternatively, as discussedab15009 hasbeenshown tobe compatible with ICC staining. If you would prefer to use this and perform an isotypecontrol to determine the specificity ofthe staining, ab18428could be used as theisotype control.

I hope thisinformation has beenof help. If you require any furtherinformation please donot hesitate to contact us again.

Thank you for getting back to me.

If you want to perform ICCto detectGlycophorin Ain human cells I would suggest ab15009 would be a good product to use for this.As you know, we do not know specifically if it detect the extracellular domain or not, but it has been used in house to perform ICC staining ofhuman cord blood cells (as presented on the datasheet of the antibody) as well as by Thorogate et al.:

Thorogate R et al. A novel fluorescence-based method in forensic science for the detection of blood in situ. Forensic Sci Int Genet 2:363-71 (2008). PubMed: 19083849

It may be that to obtain optimum staining with your specific samples you may require some optimisation of the protocol, including the permiabilisation and fixation used but the antibody is covered by the Abpromise guarentee for use with human samples with ICC. This means that if you find that it does not work, you would be entitled to a replacement antibody, refund or a credit note. More information on the Abpromise can be found from the following link:

https://www.abcam.com/abpromise

I hope this informationhas been of help.If you have any further questions please do not hesitate to contact us again.

Thank you for providing that information. I am sorry to ask further questions but it will put me in a better position to offer you advice in which of the antibodies we have which may be the most appropriate for you.

Do you want to detect the extracellular domain because that is the domain you are studying or because you think this will be the easiest to perform the ICC protocol with? Is your aim to detect the extracellular specifically, or just theglycophorin A protein using ICC?

With this additional information I will hopefully be able to assess which antibodies from our catalogue may be most suitable for your experiment.

I look forward to receiving your reply.

Thank you for contacting us and your interest in our products.

Unfortunately I am unable to provide you with any further information to that which is provided on the datasheet due to proprietary reasons. Additionally, we do not have the peptide available for sale.

If you wouldbe interested I could look intowhether we have asuitable alternative antibody where a blocking peptide is available. Inorder to do this could you please let me know what application andspecies you are looking to use with thisantibody.

I am sorry that I could not be of more help in this instance. If you have any further questions please do not hesitate to contact us again.