Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online
RecombinantRabMAb

Recombinant Anti-Glycophorin A antibody [EPR8199] (ab134111)

Reviews (1) Submit a question References (1)
Western blot - Anti-Glycophorin A antibody [EPR8199] (ab134111)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glycophorin A antibody [EPR8199] (ab134111)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glycophorin A antibody [EPR8199] (ab134111)
  • OI-RD Scanning - Anti-Glycophorin A antibody [EPR8199] (ab134111)
  • Anti-Glycophorin A antibody [EPR8199] (ab134111)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR8199] to Glycophorin A
  • Suitable for: WB, IHC-P
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

Overview

  • Product name

    Anti-Glycophorin A antibody [EPR8199]
    See all Glycophorin A primary antibodies

  • Description

    Rabbit monoclonal [EPR8199] to Glycophorin A

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-Pmore details
    Unsuitable for: ICC/IF or IP

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • Human fetal liver lysate, Human lung tissue, Human spleen tissue.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab134111 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/1000 - 1/10000. Detects a band of approximately 38 kDa (predicted molecular weight: 16 kDa).
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Notes
WB
1/1000 - 1/10000. Detects a band of approximately 38 kDa (predicted molecular weight: 16 kDa).
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Application notes
Is unsuitable for ICC/IF or IP.

Target

  • Function

    Glycophorin A is the major intrinsic membrane protein of the erythrocyte. The N-terminal glycosylated segment, which lies outside the erythrocyte membrane, has MN blood group receptors. Appears to be important for the function of SLC4A1 and is required for high activity of SLC4A1. May be involved in translocation of SLC4A1 to the plasma membrane. Is a receptor for influenza virus. Is a receptor for Plasmodium falciparum erythrocyte-binding antigen 175 (EBA-175); binding of EBA-175 is dependent on sialic acid residues of the O-linked glycans. Appears to be a receptor for Hepatitis A virus (HAV).

  • Sequence similarities

    Belongs to the glycophorin A family.

  • Post-translational
    modifications

    The major O-linked glycan are NeuAc-alpha-(2-3)-Gal-beta-(1-3)-[NeuAc-alpha-(2-6)]-GalNacOH (about 78 %) and NeuAc-alpha-(2-3)-Gal-beta-(1-3)-GalNAcOH (17 %). Minor O-glycans (5 %) include NeuAc-alpha-(2-3)-Gal-beta-(1-3)-[NeuAc-alpha-(2-6)]-GalNAcOH NeuAc-alpha-(2-8)-NeuAc-alpha-(2-3)-Gal-beta-(1-3)-GalNAcOH. About 1% of all O-linked glycans carry blood group A, B and H determinants. They derive from a type-2 precursor core structure, Gal-beta-(1,3)-GlcNAc-beta-1-R, and the antigens are synthesized by addition of fucose (H antigen-specific) and then N-acetylgalactosamine (A antigen-specific) or galactose (B antigen-specific). Specifically O-linked-glycans are NeuAc-alpha-(2-3)-Gal-beta-(1-3)-GalNAcOH-(6-1)-GlcNAc-beta-(4-1)-[Fuc-alpha-(1-2)]-Gal-beta-(3-1)-GalNAc-alpha (about 1%, B antigen-specific) and NeuAc-alpha-(2-3)-Gal-beta-(1-3)-GalNAcOH-(6-1)-GlcNAc-beta-(4-1)-[Fuc-alpha-(1-2)]-Gal-beta (1 %, O antigen-, A antigen- and B antigen-specific).

  • Cellular localization

    Cell membrane. Appears to be colocalized with SLC4A1.
  • Information by UniProt

  • Database links

    • Alternative names

      • AI853584 antibody
      • Blood group--MN locus antibody
      • CD_antigen=CD235a antibody
      • CD235a antibody
      • GLPA_HUMAN antibody
      • Glycophorin A (MNS blood group) antibody
      • Glycophorin A antibody
      • Glycophorin A, included antibody
      • Glycophorin-A antibody
      • GlycophorinA antibody
      • GPA antibody
      • GPErik antibody
      • GpMiIII antibody
      • GPSAT antibody
      • GYPA antibody
      • GYPA, included antibody
      • HGpMiIII antibody
      • HGpMiV antibody
      • HGpMiX antibody
      • HGpMiXI antibody
      • HGpSta(C) antibody
      • MN antibody
      • MN sialoglycoprotein antibody
      • MNS antibody
      • PAS-2 antibody
      • PAS2 antibody
      • Sialoglycoprotein alpha antibody
      see all

    Images

    • Western blot - Anti-Glycophorin A antibody [EPR8199] (ab134111)
      Western blot - Anti-Glycophorin A antibody [EPR8199] (ab134111)
      Anti-Glycophorin A antibody [EPR8199] (ab134111) at 1/1000 dilution + Human fetal liver tissue lysate at 10 µg

      Secondary
      Goat anti-rabbit HRP conjugated antibody at 1/2000 dilution

      Predicted band size: 16 kDa
      Observed band size: 38 kDa why is the actual band size different from the predicted?

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glycophorin A antibody [EPR8199] (ab134111)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glycophorin A antibody [EPR8199] (ab134111)

      Immunohistochemical analysis of paraffin embedded Human lung tissue labelling Glycophorin A with ab134111 at 1/100.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glycophorin A antibody [EPR8199] (ab134111)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glycophorin A antibody [EPR8199] (ab134111)

      Immunohistochemical analysis of paraffin embedded Human spleen tissue labelling Glycophorin A with ab134111 at 1/100.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • OI-RD Scanning - Anti-Glycophorin A antibody [EPR8199] (ab134111)
      OI-RD Scanning - Anti-Glycophorin A antibody [EPR8199] (ab134111)
      Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD
    • Anti-Glycophorin A antibody [EPR8199] (ab134111)
      Anti-Glycophorin A antibody [EPR8199] (ab134111)

    References (1)

    Publishing research using ab134111? Please let us know so that we can cite the reference in this datasheet.

    ab134111 has been referenced in 1 publication.

    • Lapping-Carr G  et al. Circulating extracellular vesicles from patients with acute chest syndrome disrupt adherens junctions between endothelial cells. Pediatr Res 89:776-784 (2021). PubMed: 32454519

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review

    Filter by Application

    Filter by Species

    Filter by Ratings

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Glycophorin A antibody [EPR8199]

     Inconclusive
    Abreviews
    This product is known to not work in this application or species.
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (Erythrocytes)
    Permeabilization
    Yes - Saponin 0,3%
    Specification
    Erythrocytes
    Blocking step
    0,25%BSA, 2,5%Fetal bovine serum as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 0.25% · Temperature: 4°C
    Fixative
    Paraformaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Sep 20 2019

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com