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  1. Link

    glycophorin-a-antibody-epr8200-ab129024.pdf

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RecombinantRabMAb

Recombinant Anti-Glycophorin A antibody [EPR8200] (ab129024)

  • Datasheet
  • SDS
Reviews (1)Q&A (2)References (8)

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Flow Cytometry (Intracellular) - Anti-Glycophorin A antibody [EPR8200] (ab129024)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glycophorin A antibody [EPR8200] (ab129024)
  • Western blot - Anti-Glycophorin A antibody [EPR8200] (ab129024)
  • Western blot - Anti-Glycophorin A antibody [EPR8200] (ab129024)
  • Western blot - Anti-Glycophorin A antibody [EPR8200] (ab129024)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glycophorin A antibody [EPR8200] (ab129024)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glycophorin A antibody [EPR8200] (ab129024)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glycophorin A antibody [EPR8200] (ab129024)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glycophorin A antibody [EPR8200] (ab129024)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glycophorin A antibody [EPR8200] (ab129024)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glycophorin A antibody [EPR8200] (ab129024)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glycophorin A antibody [EPR8200] (ab129024)
  • OI-RD Scanning - Anti-Glycophorin A antibody [EPR8200] (ab129024)
  • Anti-Glycophorin A antibody [EPR8200] (ab129024)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR8200] to Glycophorin A
  • Suitable for: Flow Cyt (Intra), WB, IHC-P
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

APC Carrier Free HRP PE

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Recombinant Human Glycophorin A protein (ab114330)
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Secondary
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Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

View more associated products

Overview

  • Product name

    Anti-Glycophorin A antibody [EPR8200]
    See all Glycophorin A primary antibodies
  • Description

    Rabbit monoclonal [EPR8200] to Glycophorin A
  • Host species

    Rabbit
  • Tested applications

    Suitable for: Flow Cyt (Intra), WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human Glycophorin A aa 100 to the C-terminus. The exact sequence is proprietary.
    Database link: P02724

  • Positive control

    • Fetal liver lysate, Human lung tissue, Human spleen tissue.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Dissociation constant (KD)

    KD = 2.38 x 10 -10 M
    Learn more about KD
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 0.05% BSA, 40% Glycerol
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR8200
  • Isotype

    IgG
  • Research areas

    • Cardiovascular
    • Blood
    • Blood Cell Antigens
    • RBC Antigens
    • Cardiovascular
    • Blood
    • Blood Cell Antigens
    • RBC Antigens
    • Blood Group Antigens

Associated products

  • Alternative Versions

    • Anti-Glycophorin A antibody [EPR8200] - BSA and Azide free (ab218372)
    • APC Anti-Glycophorin A antibody [EPR8200] (ab221271)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab97051)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Recombinant Protein

    • Recombinant Human Glycophorin A protein (ab114330)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab129024 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt (Intra)
1/90.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

WB
1/1000. Detects a band of approximately 38 kDa (predicted molecular weight: 16 kDa).
IHC-P (1)
1/2500 - 1/5000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

See IHC antigen retrieval protocols.

For unpurified use at 1/100 - 1/250.

Notes
Flow Cyt (Intra)
1/90.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

WB
1/1000. Detects a band of approximately 38 kDa (predicted molecular weight: 16 kDa).
IHC-P
1/2500 - 1/5000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

See IHC antigen retrieval protocols.

For unpurified use at 1/100 - 1/250.

Target

  • Function

    Glycophorin A is the major intrinsic membrane protein of the erythrocyte. The N-terminal glycosylated segment, which lies outside the erythrocyte membrane, has MN blood group receptors. Appears to be important for the function of SLC4A1 and is required for high activity of SLC4A1. May be involved in translocation of SLC4A1 to the plasma membrane. Is a receptor for influenza virus. Is a receptor for Plasmodium falciparum erythrocyte-binding antigen 175 (EBA-175); binding of EBA-175 is dependent on sialic acid residues of the O-linked glycans. Appears to be a receptor for Hepatitis A virus (HAV).
  • Sequence similarities

    Belongs to the glycophorin A family.
  • Post-translational
    modifications

    The major O-linked glycan are NeuAc-alpha-(2-3)-Gal-beta-(1-3)-[NeuAc-alpha-(2-6)]-GalNacOH (about 78 %) and NeuAc-alpha-(2-3)-Gal-beta-(1-3)-GalNAcOH (17 %). Minor O-glycans (5 %) include NeuAc-alpha-(2-3)-Gal-beta-(1-3)-[NeuAc-alpha-(2-6)]-GalNAcOH NeuAc-alpha-(2-8)-NeuAc-alpha-(2-3)-Gal-beta-(1-3)-GalNAcOH. About 1% of all O-linked glycans carry blood group A, B and H determinants. They derive from a type-2 precursor core structure, Gal-beta-(1,3)-GlcNAc-beta-1-R, and the antigens are synthesized by addition of fucose (H antigen-specific) and then N-acetylgalactosamine (A antigen-specific) or galactose (B antigen-specific). Specifically O-linked-glycans are NeuAc-alpha-(2-3)-Gal-beta-(1-3)-GalNAcOH-(6-1)-GlcNAc-beta-(4-1)-[Fuc-alpha-(1-2)]-Gal-beta-(3-1)-GalNAc-alpha (about 1%, B antigen-specific) and NeuAc-alpha-(2-3)-Gal-beta-(1-3)-GalNAcOH-(6-1)-GlcNAc-beta-(4-1)-[Fuc-alpha-(1-2)]-Gal-beta (1 %, O antigen-, A antigen- and B antigen-specific).
  • Cellular localization

    Cell membrane. Appears to be colocalized with SLC4A1.
  • Target information above from: UniProt accession P02724 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 2993 Human
    • Omim: 111300 Human
    • SwissProt: P02724 Human
    • Unigene: 434973 Human
    • Alternative names

      • AI853584 antibody
      • Blood group--MN locus antibody
      • CD_antigen=CD235a antibody
      • CD235a antibody
      • GLPA_HUMAN antibody
      • Glycophorin A (MNS blood group) antibody
      • Glycophorin A antibody
      • Glycophorin A, included antibody
      • Glycophorin-A antibody
      • GlycophorinA antibody
      • GPA antibody
      • GPErik antibody
      • GpMiIII antibody
      • GPSAT antibody
      • GYPA antibody
      • GYPA, included antibody
      • HGpMiIII antibody
      • HGpMiV antibody
      • HGpMiX antibody
      • HGpMiXI antibody
      • HGpSta(C) antibody
      • MN antibody
      • MN sialoglycoprotein antibody
      • MNS antibody
      • PAS-2 antibody
      • PAS2 antibody
      • Sialoglycoprotein alpha antibody
      see all

    Images

    • Flow Cytometry (Intracellular) - Anti-Glycophorin A antibody [EPR8200] (ab129024)
      Flow Cytometry (Intracellular) - Anti-Glycophorin A antibody [EPR8200] (ab129024)

      Overlay histogram showing K562 cells stained with unpurified ab129024 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab129024, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. 

       

       

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glycophorin A antibody [EPR8200] (ab129024)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glycophorin A antibody [EPR8200] (ab129024)

      ab129024 staining Glycophorin A in Human placenta tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed and paraffin-embedded, antigen retrieval was by heat mediation in Tris/EDTA buffer pH9. Samples were incubated with primary antibody (1/2500). ab97051(1/500) HRP-conjugated goat anti-rabbit IgG(H&L) was used as the secondary antibody. Tissue counterstained with Hematoxylin. PBS was used in the negative control rather than the Primary antibody.

    • Western blot - Anti-Glycophorin A antibody [EPR8200] (ab129024)
      Western blot - Anti-Glycophorin A antibody [EPR8200] (ab129024)
      Anti-Glycophorin A antibody [EPR8200] (ab129024) at 1/1000 dilution + Human fetal kidney at 10 µg

      Secondary
      Goat Anti-Rabbit IgG, (H+L), HRP- conjugated at 1/1000 dilution

      Predicted band size: 16 kDa

    • Western blot - Anti-Glycophorin A antibody [EPR8200] (ab129024)
      Western blot - Anti-Glycophorin A antibody [EPR8200] (ab129024)
      Anti-Glycophorin A antibody [EPR8200] (ab129024) at 1/2000 dilution + Human fetal liver lysate at 20 µg

      Secondary
      Goat Anti-Rabbit IgG, (H+L), HRP- conjugated at 1/1000 dilution

      Predicted band size: 16 kDa

    • Western blot - Anti-Glycophorin A antibody [EPR8200] (ab129024)
      Western blot - Anti-Glycophorin A antibody [EPR8200] (ab129024)
      Anti-Glycophorin A antibody [EPR8200] (ab129024) at 1/1000 dilution (unpurified) + Fetal liver lysate at 10 µg

      Predicted band size: 16 kDa
      Observed band size: 38 kDa why is the actual band size different from the predicted?

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glycophorin A antibody [EPR8200] (ab129024)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glycophorin A antibody [EPR8200] (ab129024)

      ab129024, at 1/100 dilution staining Glycophorin A in formalin fixed paraffin embedded Human lung tissue by immunohistochemistry.

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glycophorin A antibody [EPR8200] (ab129024)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glycophorin A antibody [EPR8200] (ab129024)

      ab129024, unpurified, at 1/100 dilution staining Glycophorin A in formalin fixed paraffin embedded Human spleen tissue by immunohistochemistry.

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glycophorin A antibody [EPR8200] (ab129024)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glycophorin A antibody [EPR8200] (ab129024)

      ab129024, unpurified, showing positive staining in Thyroid gland erythrocytes tissue.

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glycophorin A antibody [EPR8200] (ab129024)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glycophorin A antibody [EPR8200] (ab129024)

      ab129024, unpurified, showing negative staining in Normal brain tissue.

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glycophorin A antibody [EPR8200] (ab129024)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glycophorin A antibody [EPR8200] (ab129024)

      ab129024, unpurified, showing positive staining in Normal colon erythrocytes tissue.

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glycophorin A antibody [EPR8200] (ab129024)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glycophorin A antibody [EPR8200] (ab129024)

      ab129024, unpurified, showing positive staining in Normal kidney erythrocytes tissue.

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glycophorin A antibody [EPR8200] (ab129024)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glycophorin A antibody [EPR8200] (ab129024)

      ab129024, unpurified, showing positive staining in Normal placenta erythrocytes tissue.

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    • OI-RD Scanning - Anti-Glycophorin A antibody [EPR8200] (ab129024)
      OI-RD Scanning - Anti-Glycophorin A antibody [EPR8200] (ab129024)
      Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD
    • Anti-Glycophorin A antibody [EPR8200] (ab129024)
      Anti-Glycophorin A antibody [EPR8200] (ab129024)

    Protocols

    • Flow cytometry protocols
    • Immunohistochemistry protocols
    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (8)

    Publishing research using ab129024? Please let us know so that we can cite the reference in this datasheet.

    ab129024 has been referenced in 8 publications.

    • Llorà-Batlle O  et al. Conditional expression of PfAP2-G for controlled massive sexual conversion in Plasmodium falciparum. Sci Adv 6:eaaz5057 (2020). PubMed: 32577509
    • Morvan M  et al. Relationship of Iron Deposition to Calcium Deposition in Human Aortic Valve Leaflets. J Am Coll Cardiol 73:1043-1054 (2019). PubMed: 30846099
    • Park H  et al. Histological features of intracranial thrombi in stroke patients with cancer. Ann Neurol 86:143-149 (2019). PubMed: 31025392
    • Sundarasetty B  et al. Human Effector Memory T Helper Cells Engage with Mouse Macrophages and Cause Graft-versus-Host-Like Pathology in Skin of Humanized Mice Used in a Nonclinical Immunization Study. Am J Pathol 187:1380-1398 (2017). PubMed: 28432872
    • Kim K  et al. Histopathological Findings of Intracranial Thrombi in Nonbacterial Thrombotic Endocarditis. J Stroke 19:367-369 (2017). PubMed: 29037008
    • Ijaz F  et al. Proper cytoskeletal architecture beneath the plasma membrane of red blood cells requires Ttll4. Mol Biol Cell 28:535-544 (2017). PubMed: 27974641
    • Htun NM  et al. Near-infrared autofluorescence induced by intraplaque hemorrhage and heme degradation as marker for high-risk atherosclerotic plaques. Nat Commun 8:75 (2017). PubMed: 28706202
    • Nomani L  et al. CAL2 Immunohistochemical Staining Accurately Identifies CALR Mutations in Myeloproliferative Neoplasms. Am J Clin Pathol 146:431-8 (2016). PubMed: 27686170

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-3 of 3 Abreviews or Q&A

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Blocking step
    BSA as blocking agent for 20 minute(s) · Concentration: 1% · Temperature: 25°C
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citrate buffer, pH6.0
    Sample
    Human Tissue sections (renal clear cell carcinoma)
    Specification
    renal clear cell carcinoma
    Permeabilization
    No
    Fixative
    Formaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted May 27 2013

    Question

    In regards to the staining of the human spleen, is this staining red blood cells or lymphocytes?

    Read More

    Abcam community

    Verified customer

    Asked on Jul 24 2013

    Answer

    This antibody is staining red blood cells in our IHC data on human spleen tissue.

    Read More

    Brendan Collins

    Abcam Scientific Support

    Answered on Jul 24 2013

    Question

    This antibody did not stain red blood cells in blood smears via ICC/IF and did not IP red blood cells when conjugated to protein G bound magnetic beads;

    Read More

    Abcam community

    Verified customer

    Asked on Aug 30 2012

    Answer

    I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement for one vial of ab106101.

    To check the status of the order please contact our Customer Service team and reference this number.

    Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know.

    I wish you the best of luck with your research.

    Read More

    Abcam Scientific Support

    Answered on Aug 30 2012

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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