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    products/primary-antibodies/glycophorin-cgpc-antibody-epr4116-ab108925.pdf

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RecombinantRabMAb

Recombinant Anti-Glycophorin C/GPC antibody [EPR4116] (ab108925)

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Western blot - Anti-Glycophorin C/GPC antibody [EPR4116] (ab108925)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glycophorin C/GPC antibody [EPR4116] (ab108925)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glycophorin C/GPC antibody [EPR4116] (ab108925)
  • Flow Cytometry (Intracellular) - Anti-Glycophorin C/GPC antibody [EPR4116] (ab108925)
  • Immunoprecipitation - Anti-Glycophorin C/GPC antibody [EPR4116] (ab108925)
  • Anti-Glycophorin C/GPC antibody [EPR4116] (ab108925)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR4116] to Glycophorin C/GPC
  • Suitable for: WB, IP, IHC-P, Flow Cyt (Intra)
  • Reacts with: Human

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Overview

  • Product name

    Anti-Glycophorin C/GPC antibody [EPR4116]
    See all Glycophorin C/GPC primary antibodies
  • Description

    Rabbit monoclonal [EPR4116] to Glycophorin C/GPC
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IP, IHC-P, Flow Cyt (Intra)more details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • Flow Cyt: K562 cell lysate; IP: TF-1 lysate.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20ºC.
  • Storage buffer

    pH: 7.20
    Preservative: 0.05% Sodium azide
    Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR4116
  • Isotype

    IgG
  • Research areas

    • Cardiovascular
    • Blood
    • Blood Cell Antigens
    • RBC Antigens
    • Cardiovascular
    • Blood
    • Other
    • Cardiovascular
    • Blood
    • Blood Cell Antigens
    • RBC Antigens
    • Blood Group Antigens

Associated products

  • Alternative Versions

    • Anti-Glycophorin C/GPC antibody [EPR4116] - BSA and Azide free (ab247712)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab108925 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/1000 - 1/10000. Predicted molecular weight: 14 kDa.
IP
1/10 - 1/100.
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Flow Cyt (Intra)
1/10000.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

Notes
WB
1/1000 - 1/10000. Predicted molecular weight: 14 kDa.
IP
1/10 - 1/100.
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Flow Cyt (Intra)
1/10000.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

Target

  • Function

    This protein is a minor sialoglycoprotein in human erythrocyte membranes. The blood group Gerbich antigens and receptors for Plasmodium falciparum merozoites are most likely located within the extracellular domain. Glycophorin-C plays an important role in regulating the stability of red cells.
  • Tissue specificity

    Glycophorin-C is expressed in erythrocytes. Glycophorin-D is ubiquitous.
  • Sequence similarities

    Belongs to the glycophorin-C family.
  • Cellular localization

    Cell membrane. Linked to the membrane via band 4.1.
  • Target information above from: UniProt accession P04921 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 2995 Human
    • Omim: 110750 Human
    • SwissProt: P04921 Human
    • Unigene: 59138 Human
    • Alternative names

      • CD236 antibody
      • CD236R antibody
      • GE antibody
      • GLPC antibody
      • GLPC_HUMAN antibody
      • Glycoconnectin antibody
      • Glycophorin C (Gerbich blood group) antibody
      • Glycophorin D antibody
      • Glycophorin-C antibody
      • Glycophorin-D antibody
      • Glycoprotein beta antibody
      • GPC antibody
      • GPD antibody
      • Gypc antibody
      • GYPD antibody
      • MGC117309 antibody
      • MGC126191 antibody
      • MGC126192 antibody
      • PAS 2 antibody
      • PAS 2' antibody
      • PAS-2'' antibody
      • Sialoglycoprotein D antibody
      see all

    Images

    • Western blot - Anti-Glycophorin C/GPC antibody [EPR4116] (ab108925)
      Western blot - Anti-Glycophorin C/GPC antibody [EPR4116] (ab108925)
      All lanes : Anti-Glycophorin C/GPC antibody [EPR4116] (ab108925) at 1/1000 dilution (Purified)

      Lane 1 : TF-1 (Human Erythroleukemia erythroblast) whole cell lysate
      Lane 2 : K-562 (Human chronic myelogenous leukemia lymphoblast) whole cell lysate
      Lane 3 : Human red blood lysate

      Secondary
      All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

      Predicted band size: 14 kDa

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glycophorin C/GPC antibody [EPR4116] (ab108925)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glycophorin C/GPC antibody [EPR4116] (ab108925)

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human placenta tissue sections labeling Glycophorin C/GPC with Purified ab108925 at 1:1500 dilution (0.06 µg/ml). Heat mediated antigen retrieval was performed using Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0) . Tissue was counterstained with Hematoxylin. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) secondary antibody was used at 1:0 dilution. PBS instead of the primary antibody was used as the negative control. 

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glycophorin C/GPC antibody [EPR4116] (ab108925)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glycophorin C/GPC antibody [EPR4116] (ab108925)

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human spleen tissue sections labeling Glycophorin C/GPC with Purified ab108925 at 1:1500 dilution (0.06 µg/ml). Heat mediated antigen retrieval was performed using Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0) . Tissue was counterstained with Hematoxylin. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) secondary antibody was used at 1:0 dilution. PBS instead of the primary antibody was used as the negative control.

    • Flow Cytometry (Intracellular) - Anti-Glycophorin C/GPC antibody [EPR4116] (ab108925)
      Flow Cytometry (Intracellular) - Anti-Glycophorin C/GPC antibody [EPR4116] (ab108925)

      Flow Cytometry analysis of A549 (Human lung carcinoma epithelial cell, Left) / K-562 (Human chronic myelogenous leukemia lymphoblast, Right) cells labeling Glycophorin C/GPC with purified ab108925 at 1/10000 dilution (0.1 µg/mL) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).
      Negative control: A549.

    • Immunoprecipitation - Anti-Glycophorin C/GPC antibody [EPR4116] (ab108925)
      Immunoprecipitation - Anti-Glycophorin C/GPC antibody [EPR4116] (ab108925)

      Glycophorin C/GPC was immunoprecipitated from 0.35 mg TF-1 (Human Erythroleukemia erythroblast) whole cell lysate 10 µg with 108925 at 1/50 dilution (2µg). VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.

      Lane 1: TF-1 (Human Erythroleukemia erythroblast) whole cell lysate 10 µg

      Lane 2: ab108925 IP in TF-1 whole cell lysate

      Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab108925 in TF-1 whole cell lysate

      Blocking and dilution buffer and concentration: 5% NFDM/TBST.

      Lower band could be N-Deglycosylated GPC. (PMID: 16461900)

    • Anti-Glycophorin C/GPC antibody [EPR4116] (ab108925)
      Anti-Glycophorin C/GPC antibody [EPR4116] (ab108925)

    Protocols

    • Flow cytometry protocols
    • Immunoprecipitation protocols
    • Immunohistochemistry protocols
    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (0)

    Publishing research using ab108925? Please let us know so that we can cite the reference in this datasheet.

    ab108925 has not yet been referenced specifically in any publications.

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